RESUMEN
No specific treatment has been developed for severe fever with thrombocytopenia syndrome (SFTS). However, the prognosis can improve with early plasma exchange. Therefore, rapid and accurate detection of SFTS virus is important for diagnosis and prognosis. Direct real-time reverse transcription polymerase chain reaction (RT-PCR) testing is easier and more time-efficient than conventional real-time RT-PCR. Our study compared direct real-time RT-PCR efficiency without the RNA extraction and purification of conventional real-time RT-PCR. Samples were collected from 18 patients with SFTS and five without SFTS. A strong correlation (r = 0.774, 95% CI: 0.652-0.857, P <0.01) was found between conventional and direct real-time RT-PCR assays. Direct real-time RT-PCR showed 84.4% sensitivity and 92.0% specificity for viral detection. Direct real-time RT-PCR is an effective diagnostic tool for patients with acute phase SFTS, but further optimization is required for viral detection.
Asunto(s)
Phlebovirus , ARN Viral , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sensibilidad y Especificidad , Síndrome de Trombocitopenia Febril Grave , Humanos , Síndrome de Trombocitopenia Febril Grave/diagnóstico , Síndrome de Trombocitopenia Febril Grave/virología , Phlebovirus/genética , Phlebovirus/aislamiento & purificación , ARN Viral/aislamiento & purificación , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Masculino , Femenino , Persona de Mediana Edad , Anciano , AdultoRESUMEN
The chemical properties of thin (â¼0.6 nm) gold oxide layers prepared by an oxygen-dc glow discharge from gold films at room temperature in various solvents and solutions were studied using a surface plasmon resonance (SPR)-based optical waveguide sensing system and X-ray photoelectron spectroscopy (XPS). New insights into the stability and reactivity of gold oxides under these conditions were obtained. The O 1s XPS spectra show three oxygen species, comprising components I, II, and III in the gold oxides, and components I and II are present in this order from the top surface of the oxide (component III). The gold oxide is stable in various solvents (water, methanol, ethanol, acetone, acetonitrile, diethyl ether, chloroform, hydrocarbons) for 10 - 30 min at room temperature. The gold oxide decomposes in aqueous 10-2 M solutions of acetaldehyde, hydrochloric acid, and sodium hydroxide during these periods. Gold oxide decomposition in these solutions was monitored by SPR and the decomposition rates were obtained. Decomposition of the gold oxides was confirmed and their surfaces were characterized after decomposition by XPS.