RESUMEN
The method of rocket immunoelectrophoresis permits the detection of all antigenic admixtures in tick-borne encephalitis (TBE) vaccine. Human serum albumin constitutes the main part of protein admixtures in the preparation. Purification by microfiltration is an effective stage of the technological process of obtaining purified TBE vaccine.
Asunto(s)
Contaminación de Medicamentos , Encefalitis Transmitida por Garrapatas/inmunología , Proteínas/aislamiento & purificación , Vacunas Virales/aislamiento & purificación , Animales , Centrifugación , Embrión de Pollo , Filtración/métodos , Humanos , Sueros Inmunes/aislamiento & purificación , Inmunoelectroforesis , Ratones , Ratones Endogámicos BALB C , Filtros Microporos , Proteínas/análisis , Proteínas/inmunología , Conejos , Albúmina Sérica/análisis , Albúmina Sérica/aislamiento & purificación , Vacunas Virales/análisis , Vacunas Virales/inmunología , Vacunas Virales/normasRESUMEN
The 10466 nucleotide long sequence of the cDNA copy of the tick-borne encephalitis strain 205 viral genome has been determined. It includes the 5'-nontranslating region, the genes for structural as well as nonstructural proteins and the first 93 nucleotides of 3'-nontranslating region. The difference in amino acid sequences of structural and nonstructural proteins of strains 205. Sofjin and Neudoerfl of the tick-borne encephalitis virus and the nucleotide changes in 5'- and 3'-nontranslating of these strains are discussed.
Asunto(s)
Virus de la Encefalitis Transmitidos por Garrapatas/genética , Secuencia de Aminoácidos , Secuencia de Bases , ADN/genética , Escherichia coli/genética , Genes Bacterianos , Genes Virales , Datos de Secuencia Molecular , Mapeo Restrictivo , Homología de Secuencia de Ácido NucleicoRESUMEN
The nucleotide sequences of the cDNAs of the genes for the structural proteins C, preM, M and E of the tick-borne encephalitis viral strain 205 have been determined. The complete nucleotide sequence of the 5'-end nonstructural region of the viral genome has been studied for the first time. The difference in the amino acids sequences of the structural proteins from different strains (205, Sofiin and Neidorf) of the virus is discussed.
Asunto(s)
ADN Viral/genética , ADN/genética , Virus de la Encefalitis Transmitidos por Garrapatas/genética , Proteínas Estructurales Virales/genética , Secuencia de Aminoácidos , Secuencia de Bases , Datos de Secuencia MolecularAsunto(s)
Encefalitis Transmitida por Garrapatas/inmunología , Vacunas Virales/inmunología , Animales , Antígenos Virales/inmunología , Embrión de Pollo , Virus de la Encefalitis Transmitidos por Garrapatas/inmunología , Virus de la Encefalitis Transmitidos por Garrapatas/aislamiento & purificación , Pruebas de Hemaglutinación , RatonesRESUMEN
The split preparation, obtained by the treatment of purified and concentrated tick-borne encephalitis virus with 0.5% Tween-80 and ether and inactivated with formalin at a minimal concentration of 1:10,000, did not contain the active virus. After the removal of the detergent, the preparation retained its antigenic and immunogenic properties.
