Tratamento de cicatrizes hipertróficas com laser fracionado ablativo e drug delivery de triancinolona / Treatment of hypertrophic scars with factional ablative laser and triamcinolone drug delivery

Introdução: As cicatrizes são muito prevalentes na população mundial e podem ter impacto psicológico importante na vida do paciente. Já existem métodos clínicos e cirúrgicos convencionais para seu tratamento. Entretanto, a recidiva e os efeitos colaterais apresentados impulsionam a busca por novas modalidades terapêuticas. Objetivo: Avaliar a melhora clínica de pacientes com cicatrizes hipertróficas tratados com laser fracionado ablativo associado a drug delivery de triancinolona. Material e métodos: Foram selecionadas 18 pacientes com cicatrizes para serem tratadas, por oito semanas, com quatro sessões quinzenais de laser fracionado ablativo associado a drug delivery de triancinolona 20mg/ml. Antes e após o tratamento, o grupo foi avaliado por três examinadores por meio da evolução fotográfica, considerando-se os parâmetros aparência, discromia e grau de hipertrofia. Resultados: Completaram o estudo 15 pacientes. Destas, 35,53% apresentaram pouca ou nenhuma melhora; 37,80%, melhora moderada a intermediária; 8,89%, melhora importante e 17,80%, melhora próxima à resolução. Conclusões: A associação proposta é uma opção terapêutica promissora para o tratamento de cicatrizes hipertróficas por ser de rápida realização e cicatrização tecidual, com melhores resultados em relação às terapêuticas aplicadas isoladamente, com menores efeitos colaterais e com mais tolerabilidade pelo paciente, além de ser facilmente reproduzível em consultório.

Introduction: Scars are very prevalent in the world's population and can have a significant psychological impact in the patient's life. There are already conventional clinical and surgical methods for their treatment. However, recurrence and existing side effects drive the search for new therapeutic modalities. Objective: To evaluate the clinical improvement of patients with hypertrophic scarring treated with fractional ablative laser associated to triamcinolone drug delivery. Material and methods: Eighteen patients with scars to be treated were selected during eight weeks, with four fortnightly sessions of fractional ablative laser associated to 20mg/ml triamcinolone drug delivery. Before and after the treatment, the group was evaluated by three examiners, through the photographic evolution , considering the parameters appearance, dyschromia and degree of hypertrophy. Results: Fifteen patients completed the study. Of these, 35.53% had no or little improvement; 37.80%, moderate to intermediate improvement; 8.89%, expressive improvement and 17.80%, improvement near resolution. Conclusions: The proposed association is a promising therapeutic option for the treatment of hypertrophic scars because it is quick to perform and to heal, with less side effects and better tolerated by the patient when compared to the therapies used alone, besides being easily replicable in the office.

Population structure and genetic diversity of maize inbreds derived from tropical hybrids.

The objectives of this study were to identify the population structure and to assess the genetic diversity of maize inbreds. We genotyped 81 microsatellite loci of 90 maize inbreds that were derived from tropical hybrids and populations. The population structure analysis was based on a Bayesian approach. Each subpopulation was characterized for the effective number of alleles, gene diversity, and number of private alleles. We also performed an analysis of molecular variance and computed a measure of population differentiation (FST). The genetic distances were computed from the similarity index of Lynch and the dissimilarity measures proposed by Smouse and Peakall. The cluster analyses were based on the unweighted pair-group method using arithmetic averages and Tocher method. The clustering efficiency was assessed by the error rate of the discriminant analysis. We also performed a principal coordinates analysis. The population structure analysis revealed three tropical heterotic pools, which have been used by worldwide and Brazilian maize seed companies. The degree of genetic differentiation and of intra- and inter-population genetic diversity for these tropical heterotic pools are comparable to that observed for temperate and subtropical heterotic pools. The higher allelic frequency variation within each tropical heterotic pool and the high genetic diversity between the inbreds were evidence of heterotic groups within the main tropical heterotic pools.