RESUMEN
Here we show that interference with the integrity of the transepithelial permeability barrier of mouse mammary epithelial cells by treatment with synthetic peptides, homologous to the second extracellular domain of occludin, decreased the amount of occludin protein present at tight junctions and led to the formation of multilayered, unpolarized cell clusters. In addition, transcription of the adherens junction protein beta-catenin was induced. Following accumulation of soluble beta-catenin protein, transcription by beta-catenin/TCF/LEF was increased, as revealed by transcriptional assays following transient transfection of the reporter construct. Furthermore, treatment with occludin-II peptides up-regulated RNA levels of the known beta-catenin/TCF/LEF downstream target gene c-myc. The data presented imply a functional cross-talk between tight and adherens junctions that possibly contributes to the stepwise transformation during oncogenesis.
Asunto(s)
Proteínas del Citoesqueleto/metabolismo , Proteínas de Unión al ADN/metabolismo , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Uniones Estrechas , Transactivadores , Factores de Transcripción/metabolismo , Transcripción Genética , Animales , Northern Blotting , Cadherinas/biosíntesis , Células Cultivadas , Proteínas del Citoesqueleto/biosíntesis , Proteínas del Citoesqueleto/genética , Proteínas de Unión al ADN/genética , Epitelio/metabolismo , Femenino , Genes myc/genética , Luciferasas/metabolismo , Factor de Unión 1 al Potenciador Linfoide , Glándulas Mamarias Animales/metabolismo , Proteínas de la Membrana/biosíntesis , Ratones , Microscopía Confocal , Microscopía Fluorescente , Ocludina , Péptidos/farmacología , ARN/metabolismo , ARN Mensajero/metabolismo , Factores de Transcripción/genética , Transfección , Regulación hacia Arriba , beta CateninaRESUMEN
In epithelial cells, endocytosed transferrin and its receptor, which cycle basolaterally, have been shown to transit through recycling endosomes which can also be accessed by markers internalized from the apical surface. In this work, we have used an in vitro assay to follow transfer of an endocytosed marker from apical or basolateral early endosomes to recycling endosomes labeled with transferrin. We show that calmodulin (CaM) function is necessary for transfer and identified myr4, a member of the unconventional myosin superfamily known to use CaM as a light chain, as a possible target protein for CaM. Since myr4 is believed to act as an actin-based mechanoenzyme, we tested the role of polymerized actin in the assay. Our data show that conditions which either prevent actin polymerization or induce the breakdown of existing filaments strongly inhibit interactions between recycling endosomes and either set of early endosomes. Altogether, our data indicate that trafficking at early steps of the endocytic pathway in Madin-Darby Canine Kidney cells depends on the actin-based mechanoenzyme myr4, its light chain CaM, and polymerized actin.
Asunto(s)
Calmodulina/metabolismo , Endosomas/metabolismo , Miosina Tipo I , Miosinas/metabolismo , Actinas/metabolismo , Secuencia de Aminoácidos , Animales , Anticuerpos , Línea Celular , Polaridad Celular , Perros , Endocitosis/fisiología , Técnicas In Vitro , Fusión de Membrana/fisiología , Datos de Secuencia Molecular , Miosinas/genética , Miosinas/inmunología , Transferrina/metabolismoRESUMEN
We used flow cytometry to sort and analyze apical and basolateral endocytic vesicles from filter-grown Madin-Darby canine kidney (MDCK) cells after membrane internalization of the lipophilic fluorescent probe trimethylamino-diphenylhexatriene. Western blot analysis of sorted fractions showed enrichment of the early endosomal markers transferrin receptor and the small GTPase Rab5. Two-dimensional gel analysis indicated that the apical and basolateral early endosomes differed significantly in their protein composition. We found nine polypeptides to be specifically enriched in apical or basolateral endocytic vesicles. An apical protein identified by microsequencing was the adaptor molecule syntenin. This protein contains two PDZ domains (PSD-95, Dlg, and ZO-1 homology) that bind syndecan and ephrin-B2 cytoplasmic domains. In MDCK cells, transiently overexpressed Myc-tagged syntenin localized to both plasma membrane domains and to an intracellular vesicular compartment. Syntenin positive vesicles colocalized with internalized transferrin in the perinuclear region. In addition, syntenin colocalized in the apical supranuclear region with Rab5 and Rab11; the latter is a marker for the apical recycling endosomes in MDCK cells.
Asunto(s)
Proteínas Portadoras/metabolismo , Endocitosis , Péptidos y Proteínas de Señalización Intracelular , Riñón/metabolismo , Proteínas de la Membrana , Secuencia de Aminoácidos , Animales , Western Blotting , Línea Celular , Separación Celular , Difenilhexatrieno/análogos & derivados , Perros , Citometría de Flujo , Colorantes Fluorescentes , Riñón/citología , Orgánulos/metabolismo , SinteninasRESUMEN
CD44, the major cell surface receptor for hyaluronic acid (HA), was shown to localize to detergent-resistant cholesterol-rich microdomains, called lipid rafts, in fibroblasts and blood cells. Here, we have investigated the molecular environment of CD44 within the plane of the basolateral membrane of polarized mammary epithelial cells. We show that CD44 partitions into lipid rafts that contain annexin II at their cytoplasmic face. Both CD44 and annexin II were released from these lipid rafts by sequestration of plasma membrane cholesterol. Partition of annexin II and CD44 to the same type of lipid rafts was demonstrated by cross-linking experiments in living cells. First, when CD44 was clustered at the cell surface by anti-CD44 antibodies, annexin II was recruited into the cytoplasmic leaflet of CD44 clusters. Second, the formation of intracellular, submembranous annexin II-p11 aggregates caused by expression of a trans-dominant mutant of annexin II resulted in coclustering of CD44. Moreover, a frequent redirection of actin bundles to these clusters was observed. These basolateral CD44/annexin II-lipid raft complexes were stabilized by addition of GTPgammaS or phalloidin in a semipermeabilized and cholesterol-depleted cell system. The low lateral mobility of CD44 in the plasma membrane, as assessed with fluorescent recovery after photobleaching (FRAP), was dependent on the presence of plasma membrane cholesterol and an intact actin cytoskeleton. Disruption of the actin cytoskeleton dramatically increased the fraction of CD44 which could be recovered from the light detergent-insoluble membrane fraction. Taken together, our data indicate that in mammary epithelial cells the vast majority of CD44 interacts with annexin II in lipid rafts in a cholesterol-dependent manner. These CD44-containing lipid microdomains interact with the underlying actin cytoskeleton.