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OBJECTIVE: Carotid body tumors (CBTs) are benign but challenging. This study compared outcomes of 3 techniques of the surgical treatment of CBTs. STUDY DESIGN: This retrospective observational study was conducted from April 2013 to March 2019. The 38 patients enrolled in the study had primary tumors, including 1 with bilateral tumors and another with adrenal gland pheochromocytoma. We collected data on age, sex, size of tumor, Shamblin classification, treatment, blood loss, operative time, hospital stay, complications, and recurrence. Statistical analyses were performed using IBM SPSS Statistics version 20 software. RESULTS: Twenty-four patients were male, and 12 were female, and they ranged in age from 11 to 71 years. Cases were assigned to Shamblin groups I (n = 6), II (n = 19), and III (n = 14). Tumor size ranged from 2.0 × 2.0 cm to 5.0 × 6.0 cm. Eleven CBTs underwent blunt dissection (BD), 20 underwent BD plus resection of external carotid artery division plus vessels of encapsulation with allograft dermal matrix (BD + RECA + VE), and 8 tumors underwent surgical resection of tumors plus common carotid artery-internal carotid artery artificial vascular reconstruction (SR +C-IAVR). No perioperative death or stroke occurred. There was a significant difference between Shamblin groups I, II, and III in terms of the size of the tumor, type of treatment used, blood loss, operative time, hospital stay, and complications. Six patients had mandibular branch facial nerve transient paresis; 7 patients had hypoglossal nerve dysfunction; 3 patients had Horner syndrome; and dysphasia occurred in 2 patients. The patients were seen in follow-up for 16 to 45 months, and 1 recurrence was observed. CONCLUSIONS: Three surgical techniques-BD, BD + RECA + VE, and SR + C-IAVR-are safe and feasible for the treatment of CBTs according to Shamblin classifications.
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Tumor del Cuerpo Carotídeo , Adolescente , Adulto , Anciano , Tumor del Cuerpo Carotídeo/cirugía , Niño , Femenino , Humanos , Masculino , Persona de Mediana Edad , Recurrencia Local de Neoplasia , Estudios Retrospectivos , Resultado del Tratamiento , Procedimientos Quirúrgicos Vasculares , Adulto JovenRESUMEN
Recent studies have demonstrated that mesenchymal stem cells (MSC) exhibit a tropism to tumors and form the tumor stroma. In addition, we found that MSC can secrete different types of factors. However, the involvement of MSC-derived factors in human tongue squamous cell carcinoma (TSCC) growth has not been clearly addressed. The CCN family includes multifunctional signaling molecules that affect the initiation and development events of various tumors. In our study, we report that CCN2/connective tissue growth factor (CTGF) was the most highly induced among the CCN family members in MSC that were co-cultured with TSCC cells. To evaluate the relationship between CCN2 and TSCC growth, we downregulated MSC-derived CCN2 expression with shRNA targeting CCN2 and found that MSC-secreted CCN2 promotes TSCC cell proliferation, migration and invasion. We also confirmed that MSC-derived CCN2 partially accelerated tumor growth in vitro. Taken together, these results suggest that MSC-derived CCN2 contributes to the promotion of proliferation, migration and invasion of TSCC cells and may be a possible therapy target in the future.
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Carcinoma de Células Escamosas/genética , Movimiento Celular/genética , Proliferación Celular/genética , Factor de Crecimiento del Tejido Conjuntivo/genética , Células Madre Mesenquimatosas/metabolismo , Invasividad Neoplásica/genética , Neoplasias de la Lengua/genética , Carcinoma de Células Escamosas/patología , Línea Celular Tumoral , Regulación hacia Abajo/genética , Regulación Neoplásica de la Expresión Génica/genética , Humanos , Invasividad Neoplásica/patología , Neoplasias de la Lengua/patologíaRESUMEN
Objective @#To explore the clinical effect of personalized titanium mesh combined with free flap in the repair of maxillary defect.@*Methods@#36 cases of maxillary defect patients as the research object were selected in our hospital during May of 2010 to May of 2016. 36 defect cases were repaired with personalized titanium mesh combined with free flap, and summarize the treatment programs to explore the value of clinical application.@*Results @#By the end of follow-up, all of the flap survived, tumor recurrence rate was 5.56% with paitient satisfaction was 100%; Diplopia and dysphagia occurred in no cases; Masticatory function declined accompanied with a longer chewing time but language communication was not affected.@*Conclusion@# The application of personalized titanium mesh and free flap repair methods in maxillary defect is significant, which effectively improve the quality of life of patients whereas still have difficulties in late denture at the same time.
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Objective@#To understand the pathogenesis of bisphosphonate-related osteonecrosis of the jaws (BRONJ) and to investigate its differential diagnosis, clinical manifestations, treatment and prevention.@*Methods@#By analyzing the clinical data of 4 patients with BRONJ in the retrospective study with reviewing related literatures in the world to make a summary of it@*Results@#Cases of 4 patients mainly presented recurring pain, discharging of pus and disposure and necrosis of the bone. 3 patients received surgical and antibiotics treatments, one of them had local infection which was under control by oral antibiotic. The other 2 patients had no infection and recurrence.@*Conclusion@#BRONJ is caused by jaw necrosis due to bisphosphonate inhibition of osteoclast function. For the reason that none of the treatments is unified and satisfied, we should focus on the risk factors in prevention. Appropriate surgery treatment could be well controlled the process of the BRONJ which should be popularization in our study.
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PURPOSE: To evaluate the clinical effect of facial artery island flap for immediate reconstruction of oral and maxillofacial defects. METHODS: From March 2007 to August 2015, the clinical data of 96 patients with facial artery island flap for reconstruction of oral and maxillofacial defects were recorded and analyzed, including 46 patients undergoing anterograde facial artery flap and 50 patients undergoing reversed facial artery flap. The short-term and long-term clinical effect, including receipt site, flap survival, facial appearance and function, and postoperative complications were investigatedï¼ RESULTS: The overall success rate for facial artery island flap was 96.9%(93/96), 95.7% for anterograde flap (44/46), and 98.0% for reversed flap (49/50). All patients were followed up for six months to 6 years. The facial appearance and flap texture were satisfied. The speech and swallowing function was ideal. The scar was concealed. One patient had cervical recurrence, three had contralateral cervical lymph node metastasis. CONCLUSIONS: The facial artery island flap of submandibular area can be used to repair maxillofacial defects of medium and small sizes. The operation is simple. The reversed facial artery flap is suitable to repair upper one-third of maxillofacial defect, the survival rate is high and cosmetic outcome is ideal.
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Neoplasias de la Boca/cirugía , Procedimientos de Cirugía Plástica , Colgajos Quirúrgicos , Arterias , Humanos , Cuello , Recurrencia Local de Neoplasia , Complicaciones Posoperatorias , Cicatrización de HeridasRESUMEN
BACKGROUND: Metastasis to long distance organs is the main reason leading to morality of tongue squamous cell carcinoma (TSCC); however, the molecular mechanisms are still unknown. High mobility group AT-hook 2 (HMGA2) is highly expressed in multiple metastatic carcinomas, in which it contributes to cancer progression, metastasis and poor prognosis by upregulating Snail expression and inducing epithelial mesenchymal transition (EMT). This study focuses on investigating the role and mechanism of regulation of HMGA2 in the metastasis of TSCC. METHODS: HMGA2 mRNA and protein expression were examined in TSCC specimens by quantitative real-time polymerase chain reaction, western blotting and immunohistochemistry (IHC). Western blotting, IHC and immunofluorescence were also used to measure the expression and localization of EMT marker E-Cadherin and Vimentin both in TSCC cells and tissues. Knockdown assay was performed in vitro in TSCC cell lines using small interfering RNAs and the functional assay was carried out to determine the role of HMGA2 in TSCC cell migration and invasion. RESULTS: TSCC mRNA and protein expression were significantly up-regulated in tumor tissues when compared to adjacent non-tumor tissues, and the overexpression of HMGA2 was closely correlated with lymph nodes metastasis. Clinicopathological analysis indicated that HMGA2 expression was associated with clinical stage (P = 0.001), lymph node metastasis (P = 0.000), histological differentiation (P = 0.002) and survival (P = 0.000). Silencing the HMGA2 expression in Cal27 and UM1 resulted in the inhibition of cell migration and invasion, meanwhile down-regulation of HMGA2 impaired the phenotype of EMT in TSCC cell lines and tissues. The Multivariate survival analysis indicates that HMGA2 can be an independent prognosis biomarker in TSCC. CONCLUSION: Our findings demonstrate that HMGA2 promotes TSCC invasion and metastasis; additionally, HMGA2 is an independent prognostic factor which implied that HMGA2 can be a biomarker both for prognosis and therapeutic target of TSCC.
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Transición Epitelial-Mesenquimal/genética , Proteína HMGA2/genética , Neoplasias de la Lengua/genética , Neoplasias de la Lengua/patología , Carcinogénesis/genética , Carcinogénesis/patología , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patología , Línea Celular Tumoral , Movimiento Celular/genética , Regulación hacia Abajo , Femenino , Regulación Neoplásica de la Expresión Génica , Técnicas de Silenciamiento del Gen , Proteína HMGA2/metabolismo , Humanos , Estimación de Kaplan-Meier , Masculino , Persona de Mediana Edad , Análisis Multivariante , Invasividad Neoplásica , Fenotipo , Pronóstico , Factores de Riesgo , Factores de Transcripción de la Familia Snail , Factores de Transcripción/metabolismo , Regulación hacia Arriba/genéticaRESUMEN
BACKGROUND: The reconstruction of bilateral osteoradionecrosis (ORN) of mandibular defects using a single free bone flap is rarely performed because extensively radiated neck tissue with severe fibrosis is usually unsuitable for vascularized reconstruction. METHODS: Thirty-one patients with nasopharyngeal carcinoma (NPC) underwent bilateral reconstruction of advanced ORN in the mandible using a single fibular osteocutaneous flap. Clinical factors associated with the operation were assessed, including classification of mandible defects, types of recipient vessels, perioperative complications, and postoperative outcomes. RESULTS: All of the fibular osteocutaneous flaps survived completely, with the exception of 1 inner skin paddle that presented partial necrosis in a reconstruction of through-and-through defects. All patients experienced an improvement in cosmetic results 6 months after the reconstruction, whereas 23 patients experienced improved mouth opening compared to the preoperative condition. CONCLUSION: Advanced bilateral ORN in patients with NPC could be synchronously reconstructed with a single fibular osteocutaneous flap. © 2015 Wiley Periodicals, Inc. Head Neck 38: E-E, 2016.
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Carcinoma/cirugía , Peroné/trasplante , Mandíbula/cirugía , Neoplasias Nasofaríngeas/cirugía , Osteorradionecrosis/cirugía , Procedimientos de Cirugía Plástica , Adolescente , Adulto , Femenino , Colgajos Tisulares Libres/trasplante , Humanos , Masculino , Persona de Mediana Edad , Carcinoma Nasofaríngeo , Estudios Prospectivos , Adulto JovenRESUMEN
BACKGROUND: Selective neck dissection (SND) in clinical N0 (cN0) cases of oral squamous cell carcinoma (SCC) has been performed by surgeons using a retroauricular or modified facelift approach with robotic or endoscopic assistance. However, these procedures provide cosmetic satisfaction at the cost of possible maximal invasiveness. In this prospective study, we introduced and evaluated the feasibility as well as surgical invasiveness and cosmetic outcome of endoscopically-assisted SND via a small submandibular approach. METHODS: Forty-four patients with cT1-2N0 oral SCC (OSCC) were randomly divided into two groups of endoscopically-assisted SND and conventional SND. Perioperative and postoperative outcomes of patients were evaluated, including the length of the incision, operating time for neck dissection, estimated blood loss during the operation, amount and duration of drainage, total hospitalization period, total number of lymph nodes retrieved, satisfaction scores based on the cosmetic results, perioperative local complications, shoulder syndrome, and follow-up information. RESULTS: The mean operation time in the endoscopically-assisted group (126.04 ± 12.67 min) was longer than that in the conventional group (75.67 ± 16.67 min). However, the mean length of the incision was 4.33 ± 0.76 cm in the endoscopically-assisted SND group, and the amount and duration of drainage, total hospital stay, postoperative shoulder pain score, and cosmetic outcomes were superior in the endoscopically-assisted SND group. Additionally, the retrieved lymph nodes and complications were comparable. CONCLUSIONS: Endoscopically-assisted SND via a small submandibular approach had a longer operation time than the conventional approach. However, endoscopically-assisted SND was feasible and reliable while providing minimal invasiveness and satisfactory appearance.
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Carcinoma de Células Escamosas/cirugía , Endoscopía , Procedimientos Quirúrgicos Mínimamente Invasivos , Neoplasias de la Boca/cirugía , Disección del Cuello/métodos , Glándula Submandibular/cirugía , Carcinoma de Células Escamosas/patología , Estudios de Factibilidad , Estudios de Seguimiento , Humanos , Neoplasias de la Boca/patología , Estadificación de Neoplasias , Tempo Operativo , Procedimientos Quirúrgicos Orales , Complicaciones Posoperatorias , Pronóstico , Estudios ProspectivosRESUMEN
This retrospective clinical study assessed the reliability of the reverse facial artery flap to reconstruct the medium-sized defects in the middle facial region following cancer ablation.Fifteen medium-sized defects were repaired with reversed facial artery flap following cancer surgery. The ages of the patients ranged from 2 to 69 years; 9 were male and 6 were female. The primary lesions included palate (5 cases), maxillary gingival (6 cases), cheek or buccal mucosa (3 case), and upper lip (1 case). The size of the skin paddle varied from 4.0 cm × 6.0 cm to 5.0 cm × 10.0 cm. Direct closure was achieved at all donor sites. Fourteen of the 15 flaps survived. No donor-site problems occurred. Two patients appeared to have temporary injury of facial nerve after operation. The follow-up period ranged from 8 to 36 months; 1 patient died as a result of local recurrence and 1 patient developed cervical recurrence.Consequently, it has been demonstrated that the reversed facial artery flap had reliable blood supply and can reliably and conveniently be used for reconstruction of the medium-sized defects, especially in the middle third of oral and maxillofacial region.
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Neoplasias Faciales/cirugía , Microcirugia/métodos , Procedimientos de Cirugía Plástica/métodos , Colgajos Quirúrgicos/irrigación sanguínea , Colgajos Quirúrgicos/cirugía , Adolescente , Adulto , Anciano , Arterias/cirugía , Mejilla/cirugía , Niño , Preescolar , Femenino , Neoplasias Gingivales/cirugía , Humanos , Neoplasias de los Labios/cirugía , Masculino , Neoplasias Maxilares/cirugía , Persona de Mediana Edad , Neoplasias Palatinas/cirugía , Adulto JovenRESUMEN
OBJECTIVE: To evaluate the outcome and indication of the reconstruction of oral and maxillofacial postoperative defects by submental artery island myocutaneous flaps. METHODS: Sixty eight cases with the reconstruction of oral and maxillofacial defects by submental artery island myocutaneous flaps from January 2006 to May 2010 were analysed retrospectively. Primary lesions included carcinomas originating from tongue (28 cases), palate (13 cases), mouth floor (9 cases), gingiva (4 cases), buccal mucosa (6 cases), lip (3 cases), and other malignant or benign tumors (5 cases). The ages ranged from 25 to 84 years (mean 58 years); 47 males and 21 females. The sizes of skin paddle varied from a minimum of 4 cm × 4 cm to a maximum of 15 cm × 10 cm. RESULTS: Of the 68 flaps, 62 were survival, 4 had partial necrosis but healed with treatments, and 2 failed due to complete necrosis. Appearance and functions of recipient sites were satisfactory. The followed-up time was 3 - 24 months, local recurrence occurred in 5 cases and cervical lymph node metastases were found in 15 patients. CONCLUSION: Submental island flap is reliable for the reconstruction of postoperative defects in early oral cancer without regional lymph node metastasis or in benign tumor.
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Carcinoma de Células Escamosas/cirugía , Neoplasias de la Boca/cirugía , Procedimientos de Cirugía Plástica/métodos , Colgajos Quirúrgicos , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Trasplante de PielRESUMEN
OBJECTIVE: The purpose of this clinical study was to explore the optimal method of reconstruct mandible defects individually and immediately. STUDY DESIGN: Three-dimensional model simulation technique and vascularized fibular osteomyocutaneous flap were used to repair 15 cases of mandible defects, which were caused by ameloblastoma. A three-dimensional computed tomography (CT) images were converted to a virtual model using CAD software and the 3-dimensional (3D) simulated resin models of skeleton and ï¬bula were used to design the osteotomies, bone segment replacement and titanium mesh shaping preoperatively. RESULTS: Fibula ï¬aps were alive and no complication occurred. The patients were satisï¬ed with the results both esthetically and functionally. CONCLUSIONS: This preliminarily clinical study and case demonstrated that CAD/CAM-assisted technique with surgical treatment offers an individual anatomical reconstruction of the mandible in ameloblastoma patients. The procedures guarantee intraoperatively an exact placement of the preformed mesh even for precise reconstruction of extensive mandible defects.
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Diseño Asistido por Computadora , Reconstrucción Mandibular/métodos , Procedimientos de Cirugía Plástica/métodos , Adulto , Ameloblastoma/cirugía , Trasplante Óseo/métodos , Simulación por Computador , Estética , Femenino , Peroné/cirugía , Supervivencia de Injerto , Humanos , Imagenología Tridimensional/métodos , Masculino , Mandíbula/cirugía , Neoplasias Mandibulares/cirugía , Persona de Mediana Edad , Modelos Anatómicos , Músculo Esquelético/trasplante , Osteotomía/métodos , Planificación de Atención al Paciente , Satisfacción del Paciente , Trasplante de Piel/métodos , Colgajos Quirúrgicos/trasplante , Mallas Quirúrgicas , Tomografía Computarizada por Rayos X/métodos , Interfaz Usuario-Computador , Adulto JovenAsunto(s)
Diseño Asistido por Computadora , Mandíbula/cirugía , Procedimientos de Cirugía Plástica , Colgajos Quirúrgicos/irrigación sanguínea , Adulto , Anciano , Ameloblastoma , Femenino , Peroné , Humanos , Imagenología Tridimensional , Masculino , Mandíbula/diagnóstico por imagen , Enfermedades Mandibulares/cirugía , Neoplasias Mandibulares/cirugía , Persona de Mediana Edad , Osteorradionecrosis/cirugía , Prótesis e Implantes , Radiografía , Cirugía Asistida por Computador , Mallas Quirúrgicas , Titanio , Adulto JovenRESUMEN
INTRODUCTION: The effect of an initial radical cure and the post-operative quality of life in young patients with stage one or two tongue cancer seems to be more important than in old patients. The aim of this study was to achieve both optimal surgical effectiveness and improved post-operative quality of life for young patients with stage one or two tongue cancer. MATERIALS AND METHODS: 7 patients under 40 years of age with T(1)-T(2)N(0)M(0) squamous cell carcinoma of tongue underwent aesthetic and functional radical surgery in which reconstruction of the defect in the oral cavity and conservation of the main functional organs in neck with good scar aesthetics in the face and neck were performed. The outcomes were assessed clinically. RESULTS: No tumour recurrence was found in the tongue, floor of mouth or neck at the end of follow-up (at least 37 months). The appearance of the tongue and oral function was retained; function in face, neck and shoulder was maintained; a satisfactory cosmetic appearance of the face and neck was achieved. CONCLUSIONS: Aesthetic and functional radical surgery could ensure radical cure and unchanged, or improved post-operative, quality of life in young patients with stage one or two tongue cancer.
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Carcinoma de Células Escamosas/cirugía , Procedimientos Quirúrgicos Orales/métodos , Procedimientos de Cirugía Plástica/métodos , Colgajos Quirúrgicos , Neoplasias de la Lengua/cirugía , Adulto , Carcinoma de Células Escamosas/patología , Estética , Femenino , Peroné/cirugía , Humanos , Masculino , Disección del Cuello/métodos , Estadificación de Neoplasias , Calidad de Vida , Recuperación de la Función , Trasplante de Piel , Neoplasias de la Lengua/patologíaRESUMEN
PURPOSE: To study the changes and factors affecting the quality of life (QOL) of patients with tongue cancer 1 year after primary surgery. PATIENTS AND METHODS: A total of 289 consecutive patients with tongue cancer who had undergone primary surgery from 2003 to 2008 at our hospital were recruited. Patient QOL was evaluated using the University of Washington Quality of Life Questionnaire, version 4. Statistical analysis was conducted using a paired-samples t test and multiple stepwise linear regression with Statistical Package for Social Sciences, version 11.5 (SPSS, Chicago, IL). RESULTS: At 1 year after surgery, the appearance, activity, speech, swallowing, shoulder function, salivary, and taste domain scores were significantly lower than the preoperative scores (P < .05). However, the pain, anxiety, and mood scores were significantly better 1 year after surgery (P < .05). The overall QOL had increased greatly 1 year after surgery but did not reach the pretreatment level. Multiple stepwise linear regression analysis showed that the main factors affecting QOL were radiotherapy, advanced clinical stage (P < .05), socioeconomic status, and patient age. Radiotherapy, advanced clinical stage (P < .05), socioeconomic status, and age (P < .05) were independently associated with QOL. CONCLUSIONS: The patients with tongue cancer who have been diagnosed and treated early might have a better QOL. A greater socioeconomic status can also improve the QOL of patients with tongue cancer after primary surgery.
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Calidad de Vida , Neoplasias de la Lengua/psicología , Factores de Edad , Anciano , Análisis de Varianza , China , Femenino , Humanos , Modelos Lineales , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Periodo Posoperatorio , Radioterapia Adyuvante/efectos adversos , Recuperación de la Función , Clase Social , Estadísticas no Paramétricas , Encuestas y Cuestionarios , Neoplasias de la Lengua/radioterapia , Neoplasias de la Lengua/cirugíaRESUMEN
Survivin is a member of the inhibitor of apoptosis protein (IAP) family; it is overexpressed in most cancer tissues and induces resistance to chemotherapy. In this study, we investigated whether a short hairpin RNA (shRNA) targeting survivin can induce apoptosis and enhance chemosensitivity to cisplatin in squamous cell carcinoma of the tongue. Results showed that chemosensitivity to cisplatin was surviving dependent in three cell lines (Tca8113, Bca885, and MCF7); higher survivin mRNA expression levels were associated with lower sensitivity to cisplatin. A plasmid-containing survivin shRNA was constructed and transfected into cell line Tca8113. Survivin shRNA inhibited expression of survivin mRNA and protein (63% and 65% inhibition, respectively), significantly inhibited cell proliferation, and enhanced chemosensitivity to cisplatin (p < 0.05). Apoptosis and caspase-3 activity were induced when cells were treated with survivin shRNA and/or cisplatin. Survivin shRNA induced caspase-3-dependent apoptosis and enhanced chemosensitivity to cisplatin in these tongue squamous cell carcinoma cell lines.
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Antineoplásicos/farmacología , Carcinoma de Células Escamosas/tratamiento farmacológico , Caspasa 3/fisiología , Cisplatino/farmacología , Proteínas Asociadas a Microtúbulos/antagonistas & inhibidores , ARN Interferente Pequeño/genética , Neoplasias de la Lengua/tratamiento farmacológico , Apoptosis , Carcinoma de Células Escamosas/patología , Línea Celular Tumoral , Humanos , Proteínas Inhibidoras de la Apoptosis , Proteínas Asociadas a Microtúbulos/genética , Survivin , Neoplasias de la Lengua/patologíaRESUMEN
BACKGROUND: The mitotic Aurora-A kinase exerts crucial functions in maintaining mitotic fidelity. As a bona fide oncoprotein, Aurora-A aberrant overexpression leads to oncogenic transformation. Yet, the mechanisms by which Aurora-A enhances cancer cell survival remain to be elucidated. RESULTS: Here, we found that Aurora-A overexpression was closely correlated with clinic stage and lymph node metastasis in tongue carcinoma. Aurora-A inhibitory VX-680 suppressed proliferation, induced apoptosis and markedly reduced migration in cancer cells. We further showed that insulin-like growth factor-1, a PI3K physiological activator, reversed VX-680-decreased cell survival and motility. Conversely, wortmannin, a PI3K inhibitor, combined with VX-680 showed a synergistic effect on inducing apoptosis and suppressing migration. In addition, Aurora-A inhibition suppressed Akt activation, and VX-680-induced apoptosis was attenuated by Myr-Akt overexpression, revealing a cross-talk between Aurora-A and PI3K pathway interacting at Akt activation. Significantly, we showed that suppression of Aurora-A decreased phosphorylated Akt and was associated with increased IkappaBalpha expression. By contrast, Aurora-A overexpression upregulated Akt activity and downregulated IkappaBalpha, these changes were accompanied by nuclear translocation of nuclear factor-kappaB and increased expression of its target gene Bcl-xL. Lastly, Aurora-A overexpression induced IkappaBalpha reduction was abrogated by suppression of Akt either chemically or genetically. CONCLUSION: Taken together, our data established that Aurora-A, via activating Akt, stimulated nuclear factor-kappaB signaling pathway to promote cancer cell survival, and promised a novel combined chemotherapy targeting both Aurora-A and PI3K in cancer treatment.
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Regulación hacia Abajo/genética , Proteínas I-kappa B/metabolismo , Factor I del Crecimiento Similar a la Insulina/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Neoplasias de la Lengua/patología , Apoptosis/efectos de los fármacos , Aurora Quinasas , Carcinoma de Células Escamosas/enzimología , Carcinoma de Células Escamosas/patología , Línea Celular Tumoral , Movimiento Celular/efectos de los fármacos , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Regulación hacia Abajo/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Humanos , Metástasis Linfática/patología , Inhibidor NF-kappaB alfa , Estadificación de Neoplasias , Fosforilación/efectos de los fármacos , Piperazinas/farmacología , Unión Proteica/efectos de los fármacos , Proteínas Serina-Treonina Quinasas/antagonistas & inhibidores , Subunidades de Proteína/metabolismo , Transporte de Proteínas/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Neoplasias de la Lengua/enzimología , Factor de Transcripción ReIA/metabolismoRESUMEN
OBJECTIVE: To examine the expression of Ezrin in human salivary gland adenoid cystic carcinoma and investigate the effects of Ezrin gene silence on cell proliferation, apoptosis and invasion of adenoid cystic carcinoma (ACC)-M. METHODS: The expression of Ezrin was detected by immunohistochemistry in normal salivary gland tissue (n=15), pleomorphic adenoma (n=40) and salivary gland adenoid cystic carcinoma (n=43). The Ezrin Stealth RNAi Duplex, containing Stealth RNAi Negative Control Duplex were constructed and transfected into ACC-M cells by Lipofectamine 2000. The expression levels of Ezrin were detected by RT-PCR and immunohistochemistry. The cell cycle and apoptosis rate were analyzed by flow cytometry (FCM). The cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) and cell invasion by Transwell test. RESULTS: The positive rate of Ezrin expression in ACC was significantly higher than that in normal salivary gland tissue and pleomorphic adenoma (P<0.05). After transfection of Ezrin Stealth RNAi Duplex, the mRNA and protein expression of Ezrin were down-regulated, the cell proliferation activity was inhibited, the G0-G1 Phase cells were increased, and the apoptosis rate of Ezrin Stealth RNAi Duplex group was higher than that in control groups and cell invasion ability was decreased. CONCLUSIONS: Over expression of Ezrin in human salivary gland adenoid cystic carcinoma may promote genesis, development and metastasis of tumors. Ezrin Stealth RNAi Duplex could efficiently down-regulate the expression of Ezrin gene, and partly inhibited proliferation of ACC-M cells, induce apoptosis and decrease invasion ability of these cells in vitro.
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Carcinoma Adenoide Quístico/genética , Carcinoma Adenoide Quístico/patología , Proteínas del Citoesqueleto/genética , Neoplasias de las Glándulas Salivales/genética , Neoplasias de las Glándulas Salivales/patología , Apoptosis , Línea Celular Tumoral , Proliferación Celular , Expresión Génica , Humanos , Invasividad Neoplásica , Metástasis de la NeoplasiaRESUMEN
OBJECTIVE: To explore the application of MS-CT and 3D reconstruction in diagnosis and treatment of vascular malformations in head and neck. METHODS: 20 cases with vascular malformations in head and neck underwent MS-CT and 3-D reconstruction. Then the treatment was determined based on the results of MSCT scanning. The postoperative results were evaluated. RESULTS: The images of MS-CT showed the edge of vascular malformations partially or completely in 16 cases of venous malformations. The lesion's anatomic site and 3-D position was obtained. The 3-D images also showed the overexpanded supply arteries in 4 case of arteriovenous malformations. 2 case of venous malformations in lip underwent resection and healed completely. 12 cases of venous malformations in buccal and floor of mouth were treated with compartmentalized sclerotherapy with partial lesion involution. 2 case of venous malformations in mouth floor were treated with operation followed by sclerotherpy with partial lesion involution. 4 cases of arteriovenous malformations were treated with Superselective Artery Embolization with partial lesion involution. CONCLUSION: MS-CT and 3D reconstruction can play an important role in diagnosis and treatment of vascular malformations in head and neck.
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Imagenología Tridimensional/métodos , Malformaciones Vasculares/diagnóstico por imagen , Malformaciones Vasculares/cirugía , Adolescente , Adulto , Niño , Preescolar , Femenino , Cabeza/irrigación sanguínea , Humanos , Masculino , Cuello/irrigación sanguínea , Tomografía Computarizada Espiral , Adulto JovenRESUMEN
OBJECTIVE: To explore the effect of the patients' articulator function after reconstruction of hemi-tongue defect with forearm flap (FAP) or prime close (PC). METHODS: 36 patients who underwent hemiglossectomy were investigated after radical surgery for TSCC. 20 cases were reconstructed with FAP flaps and 16 with primary closure. The patients' articulator functions were evaluated by articulation tests. VS-9700 was used to analyze the speech character when they pronounce /ji/. RESULTS: 1) The speech articulation of patients who underwent hemi-tongue reconstruction with FAF was better than that of patients with PC, and there was significant difference between them (P < 0.05). 2) The first formant (F1) of /i/ of the PC group was lower than that of the control group (P < 0.05). But the second formant (F2) of the PC group was higher than that of the control group (P < 0.05). The first formant (F1) of /i/ of the FAF group was lower than that of the control group (P < 0.05), but there were no significant differences between FAF group and control group in F2 of /i/ (P > 0.05). CONCLUSIONS: Articulator function can be well achieved by forearm flaps reconstruction to hemi-tongue defect patients.
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Neoplasias de la Lengua/fisiopatología , Neoplasias de la Lengua/cirugía , Calidad de la Voz , Adulto , Anciano , Femenino , Antebrazo/cirugía , Humanos , Masculino , Persona de Mediana Edad , Procedimientos de Cirugía Plástica/métodos , Trasplante de Piel , Colgajos Quirúrgicos , Técnicas de SuturaRESUMEN
OBJECTIVE: To evaluate the oncologic efficacy, surgical safety, postoperative beauty, and morbidity of an ideal procedure of modified radical surgery in tongue cancer at early stages. METHODS: Six patients with early stage squamous cell carcinoma of tongue underwent a procedure of modified radical surgery, in which an inconspicuous incision was used, the external jugular vein, sternocleidomastoid muscle, spino-accessory nerve, and greater auricular nerve were preserved, half of glosso and mouth floor were excised, and the defect was repaired instantly. Recurrence of glosso, mouth floor and neck, regional edema, shoulder dysfunction, auricle sensibility, oral cavity function and beautiful outlook on the face and neck were evaluated clinically. RESULTS: Compared with classic radical neck dissection, this procedure of modified radical surgery showed an inconspicuous incision and pretty appearance, minor edema on face and neck, better shoulder function, and sensation of auricular skin. No recurred to the glosso, mouth floor, and neck was found during follow-up. The patients showed better oncological safety, pretty appearance of tongue, and better oral function of speech, swallow and mastication. CONCLUSION: This ideal procedure of modified radical surgery in tongue cancer at early stages lessens or avoids destruction of face and neck, shoulder malfunction, numbness of auricular skin, and oral dysfunction of speech, swallow and mastication without impairment on the oncologic safety of the radical surgery, and improves the quality of life of the patients.