RESUMEN
A collection of 237,954 sugarcane ESTs was examined in search of signal transduction genes. Over 3500 components involved in several aspects of signal transduction, transcription, development, cell cycle, stress responses and pathogen interaction were compiled into the Sugarcane Signal Transduction (SUCAST) Catalogue. Sequence comparisons and protein domain analysis revealed 477 receptors, 510 protein kinases, 107 protein phosphatases, 75 small GTPases, 17 G-proteins, 114 calcium and inositol metabolism proteins, and over 600 transcription factors. The elements were distributed into 29 main categories subdivided into 409 sub-categories. Genes with no matches in the public databases and of unknown function were also catalogued. A cDNA microarray was constructed to profile individual variation of plants cultivated in the field and transcript abundance in six plant organs (flowers, roots, leaves, lateral buds, and 1st and 4th internodes). From 1280 distinct elements analyzed, 217 (17%) presented differential expression in two biological samples of at least one of the tissues tested. A total of 153 genes (12%) presented highly similar expression levels in all tissues. A virtual profile matrix was constructed and the expression profiles were validated by real-time PCR. The expression data presented can aid in assigning function for the sugarcane genes and be useful for promoter characterization of this and other economically important grasses.
Asunto(s)
Perfilación de la Expresión Génica , Saccharum/genética , Transducción de Señal/genética , Transcripción Genética , Biología Computacional , Etiquetas de Secuencia Expresada , Análisis por Micromatrices , Reacción en Cadena de la Polimerasa , Reproducibilidad de los Resultados , Especificidad de la EspecieRESUMEN
A point mutation in the thi1 gene, involved in the synthesis of thiamin, has been identified in a tz-201 mutant line of Arabidopsis thaliana. The mutation occurs in a conserved protein domain and prevents the mutant plants from synthesizing thiamin. Complementation assays in yeast thi4 mutant confirm that this mutation hinders thiamin synthesis and, thus, is responsible for the tz phenotype. Northern blot analyses indicate that, in contrast to the yeast homologue, thi1 expression is not influenced by the presence of thiamin; however, reduced transcription of the gene is observed in roots and dark grown plants.