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Histological analysis is widely used to evaluate injured tendons; however, it has the limitation of being semi-quantitative. Hence, we developed a quantification method to objectively evaluate the fibrous structure of tendons, exhibiting the optical property of birefringence, using polarization-sensitive optical coherence tomography (PS-OCT). We used a partial-rupture rat model in which the middle 0.75 cm of the Achilles tendon was cut with a blade. Rats were sacrificed at 2, 4 or 6 weeks after the injury, and PS-OCT and histological analyzes were performed. The PS-OCT phase retardation images and score well represented the structural changes of the injured tendon according to the wound healing state. Therefore, the proposed novel quantification method using PS-OCT can be used to evaluate the fibrous structural status of tendons.
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Tendón Calcáneo , Tomografía de Coherencia Óptica , Ratas , Animales , Birrefringencia , Tomografía de Coherencia Óptica/métodos , Tendón Calcáneo/diagnóstico por imagen , Cicatrización de HeridasRESUMEN
Swept-source optical coherence tomography (SS-OCT) is an attractive high-speed imaging technique for retinal angiography. However, conventional swept lasers vary the cavity length of the laser mechanically to tune the output wavelength. This causes sweep-timing jitter and hence low phase stability in OCT angiography. Here, we improve an earlier phase-stabilized, akinetic, SS-OCT angiography (OCTA) method by introducing coherent averaging. We develop an active mode-locking (AML) laser as a high phase-stable akinetic swept source for the OCTA system. The phase stability of the improved system was analyzed, and the effects of coherent averaging were validated using a retina phantom. The effectiveness of the coherent averaging method was further confirmed by comparing coherently and conventionally averaged en face images of human retinal vasculature for their contrast-to-noise ratio, signal-to-noise ratio, and vasculature connectivity. The contrast-to-noise ratio was approximately 1.3 times larger when applying the coherent averaging method in the human retinal experiment. Our coherent averaging method with the high phase-stability AML laser source for OCTA provides a valuable tool for studying healthy and diseased retinas.
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Medios de Contraste , Angiografía con Fluoresceína/métodos , Vasos Retinianos/diagnóstico por imagen , Tomografía de Coherencia Óptica/métodos , Humanos , Fantasmas de Imagen , Reproducibilidad de los ResultadosRESUMEN
Herein we propose a method to mitigate a position mismatch problem for a spectral-domain polarization-sensitive optical coherence tomography (SD-PS-OCT) system that uses a single line-scan detection scheme. A single detector-based PS-OCT detects two orthogonal polarization components as two adjacent A-scan signals in turns. Thus, two adjacent A-scan signals are not scattered at a fixed point in time (position mismatch problem), resulting in uncorrelated signals between them. To achieve sequential detection of simultaneously scattered light, a buffering single-mode fiber was connected to one of the two ports coming out of the optical switch, provided a proper time delay. A single-mode optical fiber of 2.69 km in length was used to buffer, and its length was determined by a frame rate of the spectrometer used as a detector. With the proposed SD-PS-OCT scheme, the PS-OCT system with a simple configuration, and the minimized position mismatch problem between two polarization components can be set.
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The hippocampus is associated with memory and navigation, and the rodent hippocampus provides a useful model system for studying neurophysiology such as neural plasticity. Vascular changes at this site are closely related to brain diseases, such as Alzheimer's disease, dementia, and epilepsy. Vascular imaging around the hippocampus in mice may help to further elucidate the mechanisms underlying these diseases. Optical coherence tomography angiography (OCTA) is an emerging technology that can provide label-free blood flow information. As the hippocampus is a deep structure in the mouse brain, direct in vivo visualisation of the vascular network using OCTA and other microscopic imaging modalities has been challenging. Imaging of blood vessels in the hippocampus has been performed using multiphoton microscopy; however, labelling with fluorescence probes is necessary when using this technique. Here, we report the use of label-free and noninvasive microvascular imaging in the hippocampal formation of mice using a 1.7-µm swept-source OCT system. The imaging results demonstrate that the proposed system can visualise blood flow at different locations of the hippocampus corresponding with deep brain areas.
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Angiografía Cerebral/métodos , Hipocampo , Tomografía de Coherencia Óptica/métodos , Animales , Velocidad del Flujo Sanguíneo , Hipocampo/irrigación sanguínea , Hipocampo/diagnóstico por imagen , Hipocampo/crecimiento & desarrollo , Masculino , RatonesRESUMEN
We demonstrate simultaneous imaging of multiple fluorophores using wide-field epi-fluorescence microscopy with a monochrome camera. The intensities of the three lasers are modulated by a sinusoidal waveform in order to excite each fluorophore with the same modulation frequency and a different time-delay. Then, the modulated fluorescence emissions are simultaneously detected by a camera operating at four times the excitation frequency. We show that two different fluorescence beads having crosstalk can be clearly separated using digital processing based on the phase information. In addition, multiple organelles within multi-stained single cells are shown with the phase mapping method, demonstrating an improved dynamic range and contrast compared to the conventional fluorescence image. These findings suggest that wide-field epi-fluorescence microscopy with four-bucket detection could be utilized for high-contrast multicolor imaging applications such as drug delivery and fluorescence in situ hybridization.
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Thermoreflectance microscopy is essential in understanding the unpredictable local heating generation that occurs during microelectronic device operation. However, temperature measurements of multi-layered semiconductor devices represent a challenge because the thermoreflectance coefficient is quite small and is dramatically changed by the optical interference inside transparent layers of the device. Therefore, we propose a spectroscopic thermoreflectance microscopy system using a systematic approach for improving the quantitative temperature measurement of multi-layered semiconductor devices. We demonstrate the quantitative measurement of the temperature profile for physical defects on thin-film polycrystalline silicon resistors via thermoreflectance coefficient calibration and effective coefficient κ estimation.
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In breast-conserving surgery, an optical wire is a useful surgical guiding tool to optically locate small lesions within the breast tissue. However, the use of a long silica glass fiber as the optical wire can be burdensome to patients because of its stiffness and nonbiocompatibility. We investigate the use of a biocompatible fiber for light localization in tissue. A surgical suture with a diameter of 400 µm and a few centimeters long is employed as the biocompatible optical waveguide to transport the visible laser light to the inner tissue site. Optical location is confirmed with glow ball-like red laser illumination at the tip of the suture embedded within a fresh chicken breast tissue. Effective optical power coupling to the suture is made by using a double-cladding fiber coupler. From this preliminary result, we realize practical light localization with biopolymer waveguides.
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Fibras Ópticas , Imagen Óptica/instrumentación , Cirugía Asistida por Computador/instrumentación , Suturas , Animales , Pollos , Diseño de Equipo , Modelos Biológicos , Músculo Esquelético/cirugía , Imagen Óptica/métodos , Cirugía Asistida por Computador/métodosRESUMEN
We present a single-channel detection-based polarization-sensitive full-field optical coherence tomography (PS-FF-OCT) for simultaneous acquisition of high-resolution OCT and linear retardance images. A linearly polarized sub-10-fs laser was used as a broadband light source for the OCT system. A bi-stable polarization switching device, composed of a ferroelectric liquid crystal cell and an analyzer, was employed to get the horizontal and the vertical polarization components of a full-field interference signal. The time-switched two perpendicular interference signals were sequentially recorded by a single charge-coupled device camera, then processed to extract en-face functional images of a biological sample. The rat tail tendon was imaged ex vivo to confirm the imaging feasibility of the proposed system, which showed axial and transverse resolutions of 2 and 1.3 µm, respectively.
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Procesamiento de Imagen Asistido por Computador/métodos , Tomografía de Coherencia Óptica/métodos , Animales , Modelos Biológicos , Ratas , Procesamiento de Señales Asistido por Computador , Tendones/anatomía & histología , Tendones/químicaRESUMEN
We propose a label-free depth-resolved tomographic scheme for imaging a single live cell in fluid. This approach utilizes a modified time-domain full-field optical coherence tomography (FF-OCT) system combined with an optical tweezer technique. The optical trap for holding a moving specimen is made by tightly focusing a 1064 nm Q-switching pulsed laser beam with a 1.0 NA microscope objective in the sample arm of the FF-OCT part. By cosharing the probe for both systems, the optical actions of trapping and cellular resolution tomographic imaging could be achieved simultaneously. Feasibility of the combined system is demonstrated by imaging micron-sized polystyrene beads and a living suspension cell in medium.
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Pinzas Ópticas , Tomografía de Coherencia Óptica/métodos , Línea Celular Tumoral , Supervivencia Celular , Estudios de Factibilidad , Humanos , Poliestirenos/química , Agua/químicaRESUMEN
Fiber optic interferometers to sense various physical parameters including temperature, strain, pressure, and refractive index have been widely investigated. They can be categorized into four types: Fabry-Perot, Mach-Zehnder, Michelson, and Sagnac. In this paper, each type of interferometric sensor is reviewed in terms of operating principles, fabrication methods, and application fields. Some specific examples of recently reported interferometeric sensor technologies are presented in detail to show their large potential in practical applications. Some of the simple to fabricate but exceedingly effective Fabry-Perot interferometers, implemented in both extrinsic and intrinsic structures, are discussed. Also, a wide variety of Mach-Zehnder and Michelson interferometric sensors based on photonic crystal fibers are introduced along with their remarkable sensing performances. Finally, the simultaneous multi-parameter sensing capability of a pair of long period fiber grating (LPG) is presented in two types of structures; one is the Mach-Zehnder interferometer formed in a double cladding fiber and the other is the highly sensitive Sagnac interferometer cascaded with an LPG pair.
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We have experimentally implemented a multiplexible but compact fiber sensor system suitable for multipoint sensing of hydrogen gas leakage. By making dual cavities along an optical fiber and coating a palladium film only at the end of the fiber tip, the measurement errors induced by the optical source power fluctuation and the mechanical perturbation in the lead fiber could be compensated. By adjusting the length of the dual-cavity, the capability of multiplexing several hydrogen sensors could be achieved. The experiment results showed that the response speed of the sensor was increasing with temperature, but at a low temperature the response amplitude became large.
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A wavelength-division-multiplexing (WDM) coupler has been made with a bending-insensitive holey optical fiber (HOF) by using the fused biconical tapered (FBT) method. The transmission band of the proposed HOF WDM coupler could be easily tuned by adjusting the pulling length during the FBT process. Interestingly, it was observed that the air-hole structure of the HOF should be maintained to have the property of a WDM coupler. As the air holes collapse, the HOF WDM exhibits high-pass-filter-like properties. The cross-sectional scanning electron microscope images of the implemented HOF WDM coupler are presented along with the light intensity distribution measured at the coupling region of the coupler. The proposed HOF couplers may also find applications in optical coarse WDM systems and optical fiber sensors.
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We propose and demonstrate a cross-talk free simultaneous measurement system for temperature and external refractive index (ERI) implemented by dual-cavity Fabry-Perot (FP) fiber interferometer. The sensing probe consists of two cascaded FP cavities formed with a short piece of multimode fiber (MMF) and a micro-air-gap made of hollow core fiber (HOF). The fabricated sensor head was ultra-compact; the total length of the sensing part was less than 600 mum. Since the reflection spectrum of the composite FP structures is given by the superposition of each cavity spectrum, the spectrum measured in the wavelength domain was analyzed in the Fourier or spatial frequency domain. The experimental results showed that temperature could be determined independently from the spatial frequency shift without being affected by the ERI, while the ERI could be also measured solely by monitoring the intensity variation in the spatial frequency spectrum. The ERI and the temperature sensitivities were approximately 16 dB/RIU for the 1.33-1.45 index range, and 8.9 nm/ degrees C at low temperature and 14.6 nm/ degrees C at high temperature, respectively. In addition, it is also demonstrated that the proposed dual-cavity FP sensor has potential for compensating any power fluctuation that might happen in the input light source.
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Artefactos , Tecnología de Fibra Óptica/instrumentación , Dispositivos Ópticos , Refractometría/instrumentación , Termografía/instrumentación , Transductores , Diseño de Equipo , Análisis de Falla de Equipo , Miniaturización , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Integración de SistemasRESUMEN
A miniature Fabry-Perot (FP) interferometric fiber-optic sensor suitable for high-temperature sensing is proposed and demonstrated. The sensor head consists of two FP cavities formed by fusion splicing a short hollow-core fiber and a piece of single-mode fiber at a photonic crystal fiber in series. The reflection spectra of an implemented sensor are measured at several temperatures and analyzed in the spatial frequency domain. The experiment shows that the thermal-optic effect of the cavity material is much more appreciable than its thermal expansion. The temperature measurements up to 1000 degrees C with a step of 50 degrees C confirm that it could be applicable as a high-temperature sensor.
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We present an all-fiber interferometer fabricated with a single piece of an endless single-mode photonic crystal fiber (PCF) by an electric arc discharge. By forming a long period grating (LPG) at a point and collapsing the air holes at another point along the PCF, the simple but effective interferometer could be implemented. The LPG made a strong wavelength selective mode coupling between the core and cladding modes in the interesting wavelength range, while the air-hole collapse induced wavelength independent mode couplings. By cascading them, we could implement the all-fiber interferometer. As a potential application of the proposed all PCF interferometer, strain sensing is experimentally demonstrated.