Polyhydroxybutyrate (PHB) in nanoparticulate form improves physical and biological performance of scaffolds.

Polyhydroxyalkanoates (PHAs) are natural polyesters produced by microorganisms as a source of intracellular energy reserves. Due to their desirable material characteristics, these polymers have been thoroughly investigated for tissue engineering and drug delivery applications. A tissue engineering scaffold serves as a substitute of the native extracellular matrix (ECM) and plays a crucial role in tissue regeneration by providing temporary support for cells during natural ECM formation. In this study, porous, biodegradable scaffolds were prepared using native polyhydroxybutyrate (PHB) and PHB in nanoparticulate form using salt leaching method, to investigate the differences in the physicochemical properties such as crystallinity, hydrophobicity, surface morphology, roughness, and surface area and biological properties of the prepared scaffolds. As per the BET analysis, PHB nanoparticles-based (PHBN) scaffolds presented a significant difference in the surface area as compare to PHB scaffolds. PHBN scaffolds showed decreased crystallinity and improved mechanical strength as compared to PHB scaffolds. Thermogravimetry analysis shows delayed degradation of PHBN scaffolds. An examination of Vero cell lines' cell viability and adhesion over time revealed enhanced performance of PHBN scaffolds. Our research suggests that scaffold made of PHB nanoparticles could serve as a superior material for tissue engineering applications than its native form.

Scaffolds the backbone of tissue engineering: Advancements in use of polyhydroxyalkanoates (PHA).

Our body is built to heal from inside out naturally but wide-ranging medical conditions necessitate the need for artificial assistance, and therefore, something that can assist the body to heal wounds and damaged tissues quickly and efficiently is of utmost importance. Tissue engineering technology helps to regenerate new tissue to replace the diseased or injured one. The technology uses biodegradable porous three-dimensional scaffolds for mimicking the structure and functions of the natural extracellular matrix. The material and design of scaffolds are critical areas of biomaterial research. Biomaterial-based three-dimensional structures have been the most promising material to serve as scaffolds for seeding cells, both in vivo and in vitro. One such material is polyhydroxyalkanoates (PHAs) which are thermoplastic biopolyesters that are highly suitable for this purpose due to their enhanced biocompatibility, biodegradability, thermo-processability, diverse mechanical properties, non-toxicity and natural origin. Moreover, they have tremendous possibilities of customization through biological physical and chemical modification as well as blending with other materials. They are being used for several tissue engineering applications such as bone graft substitute, cardiovascular patches, stents, for nerve repair and in implantology as valves and sutures. The present review overviews usage of a multitude of PHA-based biomaterials for a wide range of tissue engineering applications, based on their properties suitable for the specific applications.

Survival of two avian respiratory viruses on porous and nonporous surfaces.

The transmission of pathogens from infected to susceptible hosts may occur through contaminated fomites and inanimate objects. This type of transmission depends on the ability of the pathogens to survive in the environment. In this report, we describe the survivability of two avian respiratory viruses, e.g., avian metapneumovirus and avian influenza virus on 12 different porous and nonporous surfaces. The viruses survived on some of the surfaces for up to 6 days postcontamination but not after 9 days. Both viruses survived longer on nonporous surfaces than on porous ones. One of the reasons for poor survival on porous surfaces could be inefficient elution of virus from these surfaces. These results should be helpful in determining how long the premises should be left vacant after an outbreak of these viruses has occurred in poultry houses.