A genome-wide association study reveals candidate genes for the supernumerary nipple phenotype in sheep (Ovis aries).

Genome-wide association studies (GWASs) have been widely applied in livestock to identify genes associated with traits of economic interest. Here, we conducted the first GWAS of the supernumerary nipple phenotype in Wadi sheep, a native Chinese sheep breed, based on Ovine Infinium HD SNP BeadChip genotypes in a total of 144 ewes (75 cases with four teats, including two normal and two supernumerary teats, and 69 control cases with two teats). We detected 63 significant SNPs at the chromosome-wise threshold. Additionally, one candidate region (chr1: 170.723-170.734 Mb) was identified by haplotype-based association tests, with one SNP (rs413490006) surrounding functional genes BBX and CD47 on chromosome 1 being commonly identified as significant by the two mentioned analyses. Moreover, Gene Ontology enrichment for the significant SNPs identified by the GWAS analysis was functionally clustered into the categories of receptor activity and synaptic membrane. In addition, pathway mapping revealed four promising pathways (Wnt, oxytocin, MAPK and axon guidance) involved in the development of the supernumerary nipple phenotype. Our results provide novel and important insights into the genetic mechanisms underlying the phenotype of supernumerary nipples in mammals, including humans. These findings may be useful for future breeding and genetics in sheep and other livestock.

Clinical risk factors for depressive symptoms in patients with epilepsy.

BACKGROUND AND AIMS: To investigate the relationships between demographic data, seizure-related factors, anti-epileptic drugs (AEDs) taking, and depressive symptoms in patients with epilepsy (PWE), determining the major clinical risk factors of depression. METHODS: Patients with epilepsy who visited our epilepsy clinic from 2010 to 2012 were included. The clinical data were collected, and Hamilton Depression Rating Scale (HAMD), National Hospital Seizure Severity Scale (NHS3) and Pittsburgh Sleep Quality Index (PSQI) were evaluated. RESULTS: A total of 116 PWE were recruited. They were divided into three groups. Age, duration of epilepsy, percentages of patients with partial seizures, history of status epilepticus (SE), using topiramate (TPM) or clonazepam (CZP), and using greater than or equal to 2 types of AEDs were all significantly higher in patients with moderate depressive symptoms than patients without depression. HAMD scores were positively correlated with age, duration of epilepsy, and the number of AEDs taking, respectively. PSQI scores were positively correlated with HAMD scores in patients with depressive symptoms. Age greater than 35 years, females, having partial seizures, history of SE, and using TPM were independent predictors of depressive symptoms in PWE by regression analysis. CONCLUSIONS: Age greater than 35 years, females, having partial seizures, history of SE, and using TPM might become risk factors for depressive symptoms in PWE.

The fate of Cu, Zn, Pb and Cd during the pyrolysis of sewage sludge at different temperatures.

In the present study, a sequential extraction procedure, recommended by the Community Bureau of Reference (BCR), was used for the fractionation of the heavy metals Cu, Zn, Pb and Cd in sewage sludge and its residues produced after pyrolysis at different temperatures from 250 to 700 degrees C. The results show that, in the sludge sample, the sum of the percentages of the reducible and oxidizable fractions for all metals except Cu was very high (65.4% for Cd, 85.7% for Pb, 78.7% for Zn), whereas the sum of the percentages of the oxidizable and residual fractions for Cu was very high (88.8%). The same result could be attained in the residues. Statistical analysis shows that at low temperatures the variation in pyrolysis temperature did not effectively contribute to the distribution of metal speciation in the residues. Meanwhile a modified Toxicity Characteristic Leaching Procedure (TCLP) was employed to determine the leachability of these four metals. The result indicates that the TCLP concentration of Cu, Zn, Pb and Cd dropped sharply after the temperature reached 350 degrees C, 550 degrees C, 500 degrees C and 400 degrees C respectively, which means pyrolysis can enhance the stability of these four metals when the temperature is high enough.