Antioxidant effects of LEDT in dystrophic muscle cells: involvement of PGC-1α and UCP-3 pathways.

PURPOSE: Reactive oxygen species and mitochondrial dysfunction play a crucial role in the pathophysiology of Duchenne muscular dystrophy (DMD). The light-emitting diode therapy (LEDT) showed beneficial effects on the dystrophic muscles. However, the mechanisms of this therapy influence the molecular pathways in the dystrophic muscles, particularly related to antioxidant effects, which still needs to be elucidated. The current study provides muscle cell-specific insights into the effect of LEDT, 48 h post-irradiation, on oxidative stress and mitochondrial parameters in the dystrophic primary muscle cells in culture. METHODS: Dystrophic primary muscle cells were submitted to LEDT, at multiple wavelengths (420 nm, 470 nm, 660 nm and 850 nm), 0.5 J dose, and evaluated after 48 h based on oxidative stress markers, antioxidant enzymatic system and biogenesis, and functional mitochondrial parameters. RESULTS: The mdx muscle cells treated with LEDT showed a significant reduction of H2O2 production and 4-HNE, catalase, SOD-2, and GR levels. Upregulation of UCP3 was observed with all wavelengths while upregulation of PGC-1α and a slight upregulation of electron transport chain complexes III and V was only observed following 850 nm LEDT. In addition, the mitochondrial membrane potential and mitochondrial mass mostly tended to be increased following LEDT, while parameters like O2·- production tended to be decreased. CONCLUSION: The data shown here highlight the potential of LEDT as a therapeutic agent for DMD through its antioxidant action by modulating PGC-1α and UCP3 levels.

Influence of Pulsed Arc Parameters on the Tig Welding Process for the Stainless Steel Duplex UNS S31803.

The influence of parameters involved in the pulsed electric arc, used as an energy source in the tungsten inert gas (TIG)-mediated welding of Duplex UNS S31803 stainless steel, to attend the manufacture of flexible pipes for the extraction of oil and gas is presented. A fundamental part in the manufacturing process of flexible pipelines is the welding of these strips so that corresponding TIG welds will be subjected to the same process and work conditions. Therefore, it is necessary to maintain the same properties in the welded regions. Covering the effects of each parameter of the pulsed electric arc such as peak and base current as welds, cyclic ratio, and pulsation frequency is a desirable endeavor. The final objective is the mitigation of problems that have a great impact on production, such as weld breakage during the conformation of the strip in the process and test failures. With this, tensile, bending, and ferrite percentage tests were performed on 12 samples that qualified as satisfactory in the visual aspect. A minimum tensile strength of 734.57 MPa and a maximum of 775.77 MPa were obtained where all values found are above the tensile strength limit of the base material of 620 MPa. With the completion of the study, it is possible to understand not only the response of the process to each parameter but also the tendency when changing them. Moreover, it is possible to explore the possibility of guiding the changes to achieve results about the visual aspect and the mechanical properties of the welded material.

Low-Level Photobiomodulation Therapy Modulates H2O2 Production, TRPC-6, and PGC-1α Levels in the Dystrophic Muscle.

Objective: This study evaluated photobiomodulation therapy (PBMT) effects on the factors involved in mitochondrial biogenesis, on the mitochondrial respiratory complexes, and on the transient receptor potential canonical channels (such as TRPC-1 and TRPC-6) in in vitro (mdx muscle cells) and in vivo studies (gastrocnemius muscle) from mdx mice, the dystrophin-deficient model of Duchenne muscular dystrophy (DMD). Background: There is no successful treatment for DMD, therefore demanding search for new therapies that can improve the muscle role, the quality of life, and the survival of dystrophic patients. Methods: The dystrophic primary muscle cells received PBMT at 0.6 J and 5 J, and the dystrophic gastrocnemius muscle received PBMT at 0.6 J. Results: The dystrophic muscle cells treated with PBMT (0.6 J and 5 J) showed no cytotoxicity and significantly lower levels in hydrogen peroxide (H2O2) production. We also demonstrated, for the first time, the capacity of PBMT, at a low dose (0.6 J), in reducing the TRPC-6 content and in raising the peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) content in the dystrophic gastrocnemius muscle. Conclusions: PBMT modulates H2O2 production, TRPC-6, and PGC-1α content in the dystrophic muscle. These results suggest that laser therapy could act as an auxiliary therapy in the treatment of dystrophic patients.

Antioxidant effects of bis-indole alkaloid indigo and related signaling pathways in the experimental model of Duchenne muscular dystrophy.

Indigo is a bis-indolic alkaloid that has antioxidant and anti-inflammatory effects reported in literature and is a promissory compound for treating chronic inflammatory diseases. This fact prompted to investigate the effects of this alkaloid in the experimental model of Duchenne muscular dystrophy. The main aim of this study was to evaluate the potential role of the indigo on oxidative stress and related signaling pathways in primary skeletal muscle cell cultures and in the diaphragm muscle from mdx mice. The MTT and Neutral Red assays showed no indigo dose-dependent toxicities in mdx muscle cells at concentrations analyzed (3.12, 6.25, 12.50, and 25.00 µg/mL). Antioxidant effect of indigo, in mdx muscle cells and diaphragm muscle, was demonstrated by reduction in 4-HNE content, H2O2 levels, DHE reaction, and lipofuscin granules. A significant decrease in the inflammatory process was identified by a reduction on TNF and NF-κB levels, on inflammatory area, and on macrophage infiltration in the dystrophic sample, after indigo treatment. Upregulation of PGC-1α and SIRT1 in dystrophic muscle cells treated with indigo was also observed. These results suggest the potential of indigo as a therapeutic agent for muscular dystrophy, through their action anti-inflammatory, antioxidant, and modulator of SIRT1/PGC-1α pathway.

A Review of the Use of Natural Fibers in Cement Composites: Concepts, Applications and Brazilian History.

The use of natural lignocellulosic fibers has become popular all over the world, as they are abundant, low-cost materials that favor a series of technological properties when used in cementitious composites. Due to its climate and geographic characteristics, Brazil has an abundant variety of natural fibers that have great potential for use in civil construction. The objective of this work is to present the main concepts about lignocellulosic fibers in cementitious composites, highlighting the innovation and advances in this topic in relation to countries such as Brazil, which has a worldwide prominence in the production of natural fibers. For this, some common characteristics of lignocellulosic fibers will be observed, such as their source, their proportion of natural polymers (biological structure of the fiber), their density and other mechanical characteristics. This information is compared with the mechanical characteristics of synthetic fibers to analyze the performance of composites reinforced with both types of fibers. Despite being inferior in tensile and flexural strength, composites made from vegetable fibers have an advantage in relation to their low density. The interface between the fiber and the composite matrix is what will define the final characteristics of the composite material. Due to this, different fibers (reinforcement materials) were analyzed in the literature in order to observe their characteristics in cementitious composites. Finally, the different surface treatments through which the fibers undergo will determine the fiber-matrix interface and the final characteristics of the cementitious composite.

Cross-talk between TRPC-1, mTOR, PGC-1α and PPARδ in the dystrophic muscle cells treated with tempol.

Background Ca2+ dysregulation and oxidative damage appear to have a central role in Duchenne muscular dystrophy (DMD) progression. The current study provides muscle cell-specific insights into the effect of Tempol on the TRPC 1 channel; on the positive and negative regulators of muscle cell differentiation; on the antioxidant enzymatic system; on the activators of mitochondrial biogenesis; and on the inflammatory process in the dystrophic primary muscle cells in culture. METHODS: Mdx myotubes were treated with Tempol (5 mM) for 24 h. Untreated mdx myotubes and C57BL/10 myotubes were used as controls. RESULTS: The Trypan Blue, MTT and Live/Dead Cell assays showed that Tempol (5 mM) presented no cytotoxic effect on the dystrophic muscle cells. The Tempol treated-mdx muscle cells showed significantly lower levels in the fluorescence intensity of intracellular calcium; TRPC-1 channel; MyoD; H2O2 and O2•- production; 4-HNE levels; SOD2, CAT and GPx levels; and TNF levels. On the other hand, SOD, CAT and GR mRNA relative expression were significantly higher in Tempol treated-mdx muscle cells. In addition, higher levels of Myogenin, MHC-Slow, mTOR, PGC-1α and PPARδ were also observed in Tempol treated-mdx muscle cells. CONCLUSION: Our findings demonstrated that Tempol decreased intracellular calcium and oxidative stress in primary dystrophic muscle cells, promoting a cross-talk between TRPC-1, mTOR, PGC-1α and PPARδ.

Multiple LEDT wavelengths modulate the Akt signaling pathways and attenuate pathological events in mdx dystrophic muscle cells.

This study is aimed at investigating the effects of LEDT, at multiple wavelengths, on intracellular calcium concentration; on transient receptor potential canonical channels; on calcium-binding protein; on myogenic factors; on myosin heavy chains; on Akt signaling pathway; on inflammatory markers; and on the angiogenic-inducing factor in dystrophic muscle cell culture experimental model. Dystrophic primary muscle cells were submitted to LEDT, at multiple wavelengths (420 nm, 470 nm, 660 nm, and 850 nm), and evaluated after 48 h for cytotoxic effects and intracellular calcium content. TRPC-1, TRPC-6, Calsequestrin, MyoD, Myogenin, MHC-slow, MHC-fast, p-AKT, p-mTOR, p-FoxO1, Myostatin, NF-κB, TNF-α, and VEGF levels were evaluated in dystrophic primary muscle cells by western blotting. The LEDT, at multiple wavelengths, treated-mdx muscle cells showed no cytotoxic effect and significant lower levels in [Ca2 +]i. The mdx muscle cells treated with LEDT showed a significant reduction of TRPC-1, NF-κB, TNF-α and MyoD levels and a significant increase of Myogenin, MHC-slow, p-AKT, p-mTOR, p-FoxO1 levels, and VEGF levels. Our findings suggest that different LEDT wavelengths modulate the Akt-signaling pathways and attenuate pathological events in dystrophic muscle cells, and a combined multiwavelength irradiation protocol may even provide a potentially therapeutic strategy for muscular dystrophies.

Prevalência da síndrome da fragilidade e perfil clínico e sociodemográfico dos idosos institucionalizados de Pindamonhangaba/SP / Prevalence of frailty syndrome and clinical and sociodemographic profile of institutionalized elderly in Pindamonhangaba/SP

A Síndrome da Fragilidade (SF) é a resposta do organismo do idoso frente ao aumento da vulnerabilidade a estressores que redundam em diminuição das reservas fisiológicas e desregulação de múltiplos sistemas. Aspectos sociais e emocionais podem potencializar essa vulnerabilidade, sendo a institucionalização do idoso um agente potencializador desse quadro. Objetivo: Estimar a prevalência da SF e caracterizar o perfil clínico e epidemiológico dos idosos institucionalizados de Pindamonhangaba/SP. Métodos: Trata-se de um estudo observacional transversal descritivo. Para tanto, foram incluídos 31 idosos de ambos os sexos, residentes em duas Instituições de Longa Permanência para Idosos (ILPI) do município de Pindamonhangaba/SP. A SF avalia: perda de peso não intencional, autorrelato de exaustão ou fadiga, diminuição da força muscular pela força de preensão palmar, lentidão na marcha e baixo nível de atividade física. Resultados: A prevalência da SF em idosos institucionalizados foi de 67,4%. Considerando os cinco itens que compõe a SF, a perda de peso não intencional esteve presente em 61,9% dos idosos, o autorrelato de exaustão ou fadiga em 66,6%, o baixo nível de atividade física em 66,6%, a lentidão na marcha em 71,4% e a diminuição da força de preensão palmar em 95,2%. A maior prevalência de idosos frágeis esteve entre os homens (41,9%), e as morbidades mais comuns foram: depressão (32,3%), hipertensão arterial sistêmica (38,7%), sintomas musculoesqueléticos (67,7%), alterações no equilíbrio (74,2%) e características sugestivas de alterações cognitivas (83,9%). Conclusão: A prevalência da SF entre os idosos do estudo foi alta com predomínio no sexo masculino. Destacou-se no perfil clínico, também, a alta prevalência, as características sugestivas de alterações cognitivas, a alteração do equilíbrio e risco de quedas e o sobrepeso ou obesidade. (AU)

Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice.

The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7-11-weeks old) mdx animals. The mdx mice were divided into three groups: mdxSed, sedentary controls (n = 28); mdxEx, exercise-trained animals (n = 28); and mdxEx+T, exercise-trained animals with the Tempol treatment (n = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-κB and increased the PGC1-α and PPARδ levels in the exercise-trained-mdx mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD.

Accidental falls in middle-aged women.

OBJECTIVE: To estimate the prevalence of accidental falls in women and to identify possible associations of sociodemographic, clinical and lifestyle variables with falls, in 2007 and 2014. METHODS: Two cross-sectional studies were performed, in 2007 and 2014, within the Projeto de Saúde de Pindamonhangaba (PROSAPIN - Pindamonhangaba Health Project), with women aged between 35 to 75 years. Probabilistic samples were selected among women living in the municipality and participating in the Health Family Strategy. Data collection included: face-to-face interview, anthropometric examination and blood test. The outcome variable "have you fallen in the last six months?" was raised during the interview. The prevalence of falls in 2007 and 2014 were estimated by score with a 95% confidence interval (95%CI). Multiple logistic regression models were constructed to identify the association of independent variables with the occurrence of falls for each year based on the odds ratio (OR). We used the Stata 14.0 software for statistical analysis. RESULTS: The prevalence of accidental falls were: 17.6% (95%CI 14.9-20.5) in 2007 and 17.2% (95%CI 14.8-19.8) in 2014. In 2007, factors associated with falls were: aged 50-64 years (OR = 1.81; 95%CI 1.17-2.80), high school (OR = 1.76; 95%CI 1.06-2.93), hyperuricemia (OR = 3.74; 95%CI 2.17-6.44), depression (OR = 2.07; 95%CI 1.31-3.27), poor sleep (OR = 1.78; 95%CI 1.12-2.82) and daytime sleepiness (OR = 1.86; 95%CI 1.16-2.99). In 2014, they were: aged 50-64 years (OR = 1.64; 95%CI 1.04-2.58), hyperuricemia (OR = 1.91; 95%CI 1.07-3.43) and depression (OR = 1.56; 95%CI 1.02-2.38), plus metabolic syndrome (OR = 1.60; 95%CI 1.03-2.47) and musculoskeletal pain (OR = 1.81; 95%CI 1.03-3.18). CONCLUSIONS: Falls occur significantly in women aged 50 years or over, indicating that they are not restricted to older adults and that there is a need to initiate preventive measures earlier. Both studies showed similar magnitudes of occurrence of accidental falls and reinforced their multifactorial nature. In addition, hyperuricemia may be a potential new factor associated with falls.

Tempol improves redox status in mdx dystrophic diaphragm muscle.

Oxidative stress is a critical element in relationship to the pathophysiology of Duchenne muscular dystrophy (DMD). In the mice the diaphragm (DIA) is most resembles the dystrophic human pathology. In this study we have evaluated the consequences of a synthetic antioxidant (tempol) on oxidative stress parameters in the DIA muscle of mdx mice. The mdx mice were separated into two groups: mdx, the control group receiving intraperitoneal (i.p.) injections of saline solution (100 µL), and mdxT, the treated group receiving i.p. injections of tempol (100 mg/kg). The tempol-treated group showed reduced oxidative stress markers, decreasing the dihydroethidium reaction (DHE) area; autofluorescent lipofuscin granules; and 4-hydroxynonenal (4-HNE)-protein adduct levels. DIA muscle of mdx mice. At the same time, the manganese-superoxide dismutase 2 (SOD2) levels were increased in the tempol-treated group. In addition, the tempol-treated group showed reduced levels of glutathione-disulphide reductase (GSR), glutathione peroxidase 1 (GPx1) and catalase (CAT) in immunoblots. The tempol-treated group has also shown lower relative gene expression of SOD1, CAT and GPx than the non-treated group. Our data demonstrated that tempol treatment reduced oxidant parameters and increased anti-oxidant SOD2 levels in the DIA muscle of mdx mice, which may contribute to the normalization of the redox homeostasis of dystrophic muscles.

Accidental falls in middle-aged women / Quedas acidentais em mulheres de meia-idade

ABSTRACT OBJECTIVE To estimate the prevalence of accidental falls in women and to identify possible associations of sociodemographic, clinical and lifestyle variables with falls, in 2007 and 2014. METHODS Two cross-sectional studies were performed, in 2007 and 2014, within the Projeto de Saúde de Pindamonhangaba (PROSAPIN - Pindamonhangaba Health Project), with women aged between 35 to 75 years. Probabilistic samples were selected among women living in the municipality and participating in the Health Family Strategy. Data collection included: face-to-face interview, anthropometric examination and blood test. The outcome variable "have you fallen in the last six months?" was raised during the interview. The prevalence of falls in 2007 and 2014 were estimated by score with a 95% confidence interval (95%CI). Multiple logistic regression models were constructed to identify the association of independent variables with the occurrence of falls for each year based on the odds ratio (OR). We used the Stata 14.0 software for statistical analysis. RESULTS The prevalence of accidental falls were: 17.6% (95%CI 14.9-20.5) in 2007 and 17.2% (95%CI 14.8-19.8) in 2014. In 2007, factors associated with falls were: aged 50-64 years (OR = 1.81; 95%CI 1.17-2.80), high school (OR = 1.76; 95%CI 1.06-2.93), hyperuricemia (OR = 3.74; 95%CI 2.17-6.44), depression (OR = 2.07; 95%CI 1.31-3.27), poor sleep (OR = 1.78; 95%CI 1.12-2.82) and daytime sleepiness (OR = 1.86; 95%CI 1.16-2.99). In 2014, they were: aged 50-64 years (OR = 1.64; 95%CI 1.04-2.58), hyperuricemia (OR = 1.91; 95%CI 1.07-3.43) and depression (OR = 1.56; 95%CI 1.02-2.38), plus metabolic syndrome (OR = 1.60; 95%CI 1.03-2.47) and musculoskeletal pain (OR = 1.81; 95%CI 1.03-3.18). CONCLUSIONS Falls occur significantly in women aged 50 years or over, indicating that they are not restricted to older adults and that there is a need to initiate preventive measures earlier. Both studies showed similar magnitudes of occurrence of accidental falls and reinforced their multifactorial nature. In addition, hyperuricemia may be a potential new factor associated with falls.

RESUMO OBJETIVO Estimar a prevalência de quedas acidentais em mulheres e identificar possíveis associações de variáveis sociodemográficas, clínicas e de hábitos de vida com as quedas, em 2007 e 2014. MÉTODOS Foram realizados dois estudos transversais, em 2007 e 2014, dentro do Projeto de Saúde de Pindamonhangaba (PROSAPIN), com mulheres com idades variando de 35 a 75 anos. As amostras probabilísticas foram selecionadas dentre as mulheres residentes no município e participantes da Estratégia Saúde da Família. A coleta de dados incluiu: entrevista face a face, exame antropométrico e exame sanguíneo. A variável de desfecho "Sofreu queda nos últimos seis meses?" foi levantada durante a entrevista. Foram estimadas as prevalências de quedas em 2007 e 2014 por ponto e intervalo de confiança de 95% (IC95%). Modelos de regressão logística múltipla foram construídos para identificar a associação das variáveis independentes e a ocorrência de quedas para cada ano a partir da odds ratio (OR). Utilizou-se o software Stata 14.0 para análise estatística. RESULTADOS As prevalências de quedas acidentais foram: 17,6% (IC95% 14,9-20,5) em 2007 e 17,2% (IC95% 14,8-19,8) em 2014. Em 2007 os fatores associados a quedas foram: idade de 50-64 anos (OR = 1,81; IC95% 1,17-2,80), ensino médio (OR = 1,76; IC95% 1,06-2,93), hiperuricemia (OR = 3,74; IC95% 2,17-6,44), depressão (OR = 2,07; IC95% 1,31-3,27), sono ruim (OR = 1,78; IC95% 1,12-2,82) e sonolência diurna (OR = 1,86; IC95% 1,16-2,99). Em 2014 permaneceram: idade de 50-64 anos (OR = 1,64; IC95% 1,04-2,58), hiperuricemia (OR = 1,91; IC95% 1,07-3,43) e depressão (OR = 1,56; IC95% 1,02-2,38), acrescidos da síndrome metabólica (OR = 1,60; IC95% 1,03-2,47) e da dor musculoesquelética (OR = 1,81; IC95% 1,03-3,18). CONCLUSÕES As quedas ocorrem de maneira importante em mulheres a partir dos 50 anos, indicando que não são restritas a idosos e que há necessidade de iniciar medidas preventivas mais precocemente. Os dois estudos mostraram magnitudes semelhantes de ocorrência de quedas acidentais e reforçaram sua multifatorialidade. Além disso, a hiperuricemia pode ser um potencial novo fator associado a quedas.

Coenzyme Q10 supplementation acts as antioxidant on dystrophic muscle cells.

Increased oxidative stress is a frequent feature in Duchenne muscular dystrophy (DMD). High reactive oxygen species (ROS) levels, associated with altered enzyme antioxidant activity, have been reported in dystrophic patients and mdx mice, an experimental model of DMD. In this study, we investigated the effects of coenzyme Q10 (CoQ10) on oxidative stress marker levels and calcium concentration in primary cultures of dystrophic muscle cells from mdx mice. Primary cultures of skeletal muscle cells from C57BL/10 and mdx mice were treated with coenzyme Q10 (5 µM) for 24 h. The untreated mdx and C57BL/10 muscle cells were used as controls. The MTT and live/dead cell assays showed that CoQ10 presented no cytotoxic effect on normal and dystrophic muscle cells. Intracellular calcium concentration, H2O2 production, 4-HNE, and SOD-2 levels were higher in mdx muscle cells. No significant difference in the catalase, GPx, and Gr levels was found between experimental groups. This study demonstrated that CoQ10 treatment was able to reduce levels of oxidative stress markers, such as H2O2, acting as an antioxidant, as well as decreasing abnormal intracellular calcium influx in dystrophic muscles cells. This study demonstrated that CoQ10 treatment was able to reduce levels of oxidative stress markers, such as H2O2, acting as an antioxidant, as well as decreasing abnormal intracellular calcium influx in dystrophic muscles cells. Our findings also suggest that the decrease of oxidative stress reduces the need for upregulation of antioxidant pathways, such as SOD and GSH.

Detraining and Anabolic-Androgenic Steroid Discontinuation Change Calcaneal Tendon Morphology.

Several side effects of anabolic-androgenic steroid (AAS) administration associated with training are reported in the biomechanical properties of the calcaneal tendon (CT) of rats. Thus, the aim of the present study is to evaluate the effects of the detraining and discontinuation of AAS administration on the CT morphology of rats submitted to exercise in water. Animals were divided into two groups (20/group): (1) Immediately after training (IA), and (2) Six weeks of detraining and AAS discontinuation (6W). The IA group included four subgroups: Sedentary (S), Trained (T), Sedentary with AAS administration (SAAS), and trained with AAS administration (TAAS). The 6W group included four subgroups: Sedentary (6W-S), six weeks of detrained (6W-T), six weeks of sedentary with AAS discontinuation (6W-SAAS), and six weeks of detrained with AAS discontinuation (6W-TAAS). Data show significant reduction in adipose cells volume density (Vv%) in the distal CT in 6W-TAAS group, indicating that training can exert a positive effect on the tendon. The 6W-SAAS group exhibited increased adipose cells Vv% in the distal region, compared with the W6-S and W6-T groups. A decrease in tendon proper cells Vv% and in peritendinous sheath cells Vv% of proximal and distal regions was also observed. In 6W-TAAS group showed increase in adipose cells, blood vessels, peritendinous sheath cells, and tendon proper cells Vv% in the distal region of the CT. The vertical jumps in water were not able to protect CT regions from the negative effects of AAS discontinuation for six weeks. However, after detraining and AAS discontinuation, many protective factors of the mechanical load in the long-term could be observed.

Significados, para os familiares, de conviver com um idoso com sequelas de Acidente Vascular Cerebreal (AVC) / Meanings to family members living with an elderly affected by stroke sequelae / Significados para los familiares de convivir con un anciano con secuelas de Accidente Cerebrovascular (AVC)

O objetivo deste estudo é identificar os significados para os familiares de conviver com idosos com sequelas de AVC. Os participantes do estudo foram os familiares que conviviam com idoso do gênero masculino e feminino. A amostra foi constituída por 15 membros familiares. Os instrumentos utilizados para a coleta de dados foram questionário referente à caracterização pessoal e familiar dos participantes e o roteiro de entrevista semiestruturada. Para extração das ideias principais, foi utilizada a Análise de Conteúdo de Bardin. As categorias emergentes do tema 'Significado de conviver' foram 'Experiência dolorosa', 'Mudança de hábito e de vida', 'Uma situação muito difícil', 'Ser resiliente' e 'Dificuldade da pessoa em aceitar a doença'. Conclui-se que conviver com idosos com sequelas de AVC assume caráter com implicações físico-psicológicas e familiares.(AU)

The objective of this study is to identify the meanings for relatives who live with an elderly with stroke sequelae. The study participants were family members cohabiting with elderly men and women with stroke sequelae. The sample consisted of 15 family members. Data was gathered using a questionnaire on personal and family characteristics of the participants and semi-structured interviews. We used the Bardin Content Analysis in order to extract the main ideas. The emerging categories for the theme 'meaning of living together' were 'a painful experience', 'habits and life changes', 'a very difficult situation', 'to be resilient' and 'the difficulty of the individual in accepting the disease'. We conclude that living with elderly individuals with stroke sequelae has physical, psychological and familial implications.(AU)

El objetivo de este estudio es identificar los significados para los familiares de convivir con una persona anciana de la familia con secuelas de AVC. Los participantes del estudio fueron los familiares que convivían con un anciano con secuelas de AVC del genero masculino y femenino. La muestra estaba formada por 15 miembros de la familia. Los instrumentos utilizados para la colecta de datos fueron: cuestionario referente a la caracterización personal y familiar de los participantes y el guión de entrevista semi-estructurada. Para la extracción de las ideas principales se utilizó el Análisis de Contenido de Bardin. As categorías emergentes del tema 'Significado de convivir' fueron: 'Experiencia dolorosa', 'Cambio de hábito y de vida, 'Una situación muy difícil', 'Ser resiliente' y 'Dificultad de la persona para aceptar la enfermedad. Se concluye que convivir con ancianos con secuelas de AVC asume carácter con implicaciones físicas, psicológicas y familiares.(AU)

Jovens universitários da área da saúde são vulneráveis ao HIV / Undergraduate students of health area are vulnerable to HIV / Estudiantes universitarios del área de salud son vulnerables al VIH

Objetivos: O objetivo do presente trabalho foi analisar a vulnerabilidade ao HIV de jovens estudantes universitários. Métodos: Trata-se de pesquisa descritiva tipo levantamento realizada com estudantes universitários de ambos os sexos (n=243), com idade entre 15 a 24 anos (19,23±1,54). Aplicamos o questionário: "Eu preciso fazer o teste do HIV/AIDS?", adicionando questões sobre idade, sexo e relação sexual mais frequente. Resultados: Na amostra, 73,20% eram do sexo feminino (n=177). A relação sexual mais frequente foi heterossexual (66,70%), seguida pelo homossexual (7,70%) e bissexual (1,20%). Cerca de 23,60% não iniciaram a vida sexual. Encontramos 93,80% da amostra com média ou muita vulnerabilidade ao HIV, sendo 39,10% muito vulnerável, sem diferença significativa (p=0,800) entre sexos. Foi encontrada relação (p=0,000) entre o aumento da idade e maior vulnerabilidade, sendo que 57,9% dos homossexuais e 100% dos bissexuais são muito vulneráveis. Conclusão: A totalidade da população de jovens universitários estudada é vulnerável ao HIV. Embora a amostra tenha acesso ao conhecimento, não o utiliza na prática, sugerindo uma percepção de invulnerabilidade deste grupo.

Objective: The aim of the present study was to analyze the vulnerability to HIV of young undergraduate students. Methods: This was a descriptive survey carried out with undergraduate students of both sexes (n =243), aged between 15 and 24 years (19.23 ± 1.54). We applied the questionnaire: "Do I need to be tested for HIV/AIDS?", adding questions about age, sex and more frequent sexual intercourse. Results: In the sample, 73.2% were female (n=177). The most frequent sexual intercourse was heterosexual (66.7%), followed by homosexual (7.7%) and bisexual (1.2%). About 23.6% did not initiate sex life. We found 93.8% of the sample with medium or highly vulnerable to HIV, being 39.1% with high vulnerability, with no significant difference (p=0.8) between sexes. There was a relationship (p=0) between increasing age and greater vulnerability, with 57.9% of homosexuals and 100% of bisexuals with high vulnerability. Conclusion: Although the sample has access to knowledge, it does not use it in practice, suggesting a perception of invulnerability of this group.

Objetivos: El objetivo del presente trabajo fue analizar la vulnerabilidad al VIH de los estudiantes universitarios jóvenes. Métodos: Se trata de una encuesta descriptiva realizada con estudiantes de ambos sexos (n=243), con edades comprendidas entre 15 y 24 años (19,23 ± 1,54). Hemos aplicado el cuestionario: "¿Tengo que hacerme la prueba del VIH/SIDA?", Añadiendo preguntas sobre la edad, el sexo y las relaciones sexuales más frecuentes. Resultados: En la muestra, el 73,20% eran mujeres (n = 177). Las relaciones sexuales más frecuentes fueron heterosexuales (66,70%), seguidas de homosexuales (7,70%) y bisexuales (1,20%). Alrededor del 23,60% no inició la vida sexual. Encontramos 93,80% de la muestra con una alta o media vulnerabilidad al VIH, siendo 39,10% con alta vulnerabilidad, sin diferencias significativas (p=0,800) entre los sexos. Hubo una relación (p=0,000) entre el aumento de la edad y mayor vulnerabilidad, con el 57,90% de los homosexuales y el 100% de los bisexuales con alta vulnerabilidad. Conclusión: Aunque la muestra tiene acceso al conocimiento, no lo utiliza en la práctica, sugiriendo una percepción de invulnerabilidad de este grupo.

Meanings attributed by family members to the act of caring for elderly people with stroke sequels / Significados para os familiares em cuidar de um idoso sequelado de AVC

The objective of this study was to identify meanings that family members attribute to the act of caring for elderly individuals with stroke sequels. The participants were family members that were taking care of a senior with stroke sequels, both male and female. The sample was composed of 15 family members. The instruments used for data collection were: a questionnaire referring to the participants' personal and family profile, in addition to a semi-structured interview script. For extraction of main ideas, Bardin's content analysis was used. The categories that emerged from the 'Meaning of caring' theme were: 'A lot of love and support', 'Hard because of the person's dependence', 'Caring is not hard', 'Responsibility' and 'Role change'. The conclusion is that caring for an elderly person with stroke sequels involves a variety of aspects and situations.

O objetivo deste estudo foi identificar os significados para os familiares em cuidar de um idoso sequelado de AVC. Os participantes do estudo foram os familiares que cuidavam de um idoso sequelado de AVC, tanto do gênero masculino quanto do feminino. A amostra foi constituída por 15 membros familiares. Os instrumentos utilizados para a coleta de dados foram: questionário referente à caracterização pessoal e familiar dos participantes e o roteiro de entrevista semiestruturada. Para extração das ideias principais, foi utilizada a Análise de Conteúdo de Bardin. As categorias emergentes do tema 'Significado de cuidar' foram: 'Muito amor e apoio', 'Difícil pela dependência da pessoa', 'Não é difícil cuidar', 'Responsabilidade' e 'Inversão de papéis'. Conclui-se que cuidar de um idoso sequelado de AVC assume diversos aspectos e situações.

Prevalence and factors associated with urinary incontinence in climacteric / Prevalência e fatores associados à incontinência urinária no climatério

SUMMARY Objective: To estimate the prevalence and identify associated factors to urinary incontinence (UI) in climacteric women. Method: In a cross-sectional study with a stratified random sample, 1,200 women aged between 35 and 72 years were studied, enrolled in the Family Health Strategy in the city of Pindamonhangaba, São Paulo. Urinary incontinence was investigated using the International Consultation of Incontinence Questionnaire - Short Form, while associated factors were assessed based on a self-reported questionnaire with socio-demographic, obstetric and gynecological history, morbidities and drug use. The prevalence of urinary incontinence was estimated with a 95% confidence interval (95CI) and the associated factors were identified through multiple logistic regression model performed using Stata software, version 11.0. Results: Women had a mean age of 51.9 years, most were in menopause (59.4%), married (87.5%), Catholic (48.9%), and declared themselves black or brown (47.2%). The mean age of menopause of women with UI was 47.3 years. The prevalence of UI was 20.4% (95CI: 17.8-23.1%). The factors associated with UI were urinary loss during pregnancy (p=0.000) and after delivery (p=0.000), genital prolapse (p=0.000), stress (p=0.001), depression (p=0.002), and obesity (p=0.006). Conclusion: The prevalence of UI was lower but similar to that found in most similar studies. Factors associated with the genesis of UI were urinary loss during pregnancy and after delivery, genital prolapse and obesity.

RESUMO Objetivo: estimar a prevalência e identificar os possíveis fatores associados à incontinência urinária (IU) em mulheres no climatério. Método: em estudo analítico transversal com amostra aleatória estratificada, foram estudadas 1.200 mulheres, entre 35 e 72 anos, cadastradas na Estratégia de Saúde da Família do município de Pindamonhangaba, SP. A IU foi investigada por meio do International Consultation of Incontinence Questionnaire – Short Form e os fatores associados, por meio de questionário autorreferido, contendo informações sociodemográficas, história ginecológica e obstétrica, morbidades e uso de medicamentos. Estimou-se a prevalência da incontinência urinária com intervalo de confiança de 95% (IC95%), e os fatores associados foram identificados por meio de um modelo de regressão logística múltipla realizada no Programa Stata, versão 11.0. Resultados: as mulheres apresentavam média etária de 51,9 anos, estavam na menopausa (59,4%), eram casadas (87,5%), católicas (48,9%) e declararam-se negras ou pardas (47,2%). A média de idade da menopausa das mulheres com IU foi de 47,3 anos. A prevalência de IU foi de 20,4% (IC95%: 17,8-23,1). Os fatores associados à IU foram perda urinária na gestação (p=0,000) e no pós-parto (p=0,000), prolapso genital (p=0,000), estresse (p=0,001), depressão (p=0,002) e obesidade (p=0,006). Conclusão: a prevalência de IU foi inferior, mas semelhante à encontrada na maioria dos estudos análogos. Os fatores associados à gênese da IU foram perda urinária na gestação e no pós-parto, prolapso genital e obesidade.

Prevalence and factors associated with urinary incontinence in climacteric.

OBJECTIVE: To estimate the prevalence and identify associated factors to urinary incontinence (UI) in climacteric women. METHOD: In a cross-sectional study with a stratified random sample, 1,200 women aged between 35 and 72 years were studied, enrolled in the Family Health Strategy in the city of Pindamonhangaba, São Paulo. Urinary incontinence was investigated using the International Consultation of Incontinence Questionnaire - Short Form, while associated factors were assessed based on a self-reported questionnaire with socio-demographic, obstetric and gynecological history, morbidities and drug use. The prevalence of urinary incontinence was estimated with a 95% confidence interval (95CI) and the associated factors were identified through multiple logistic regression model performed using Stata software, version 11.0. RESULTS: Women had a mean age of 51.9 years, most were in menopause (59.4%), married (87.5%), Catholic (48.9%), and declared themselves black or brown (47.2%). The mean age of menopause of women with UI was 47.3 years. The prevalence of UI was 20.4% (95CI: 17.8-23.1%). The factors associated with UI were urinary loss during pregnancy (p=0.000) and after delivery (p=0.000), genital prolapse (p=0.000), stress (p=0.001), depression (p=0.002), and obesity (p=0.006). CONCLUSION: The prevalence of UI was lower but similar to that found in most similar studies. Factors associated with the genesis of UI were urinary loss during pregnancy and after delivery, genital prolapse and obesity.

Regulação e ativação das células satélites durante a regeneração muscular / Regulation and activation of satellite cells during musculoskeletal regeneration

Os avanços da biologia molecular, biologia celular e genética tem melhorado muito a nossa compreensão da biologia do músculo esquelético, demonstrando que as características biológicas das células satélites (CSs) são fundamentais no processo de regeneração muscular. O potencial terapêutico destas células vem sendo discutido pela literatura, por isso o entendimento do metabolismo e a regulação das células precursoras das fibras musculares merece atenção. Assim, o objetivo desta revisão narrativa é apresentar aspectos relacionados aos fatores de regulação das células satélites durante o processo de regeneração do músculo esquelético. A regeneração do músculo esquelético é um processo orquestrado que envolve a ativação e diferenciação das CSs. As interações celulares ocorrem com os diversos fatores miogênicos, de crescimento e hormonais. Entre eles destacam-se o HGF (fator de crescimento hepatocitário), IGF (fator de crescimento semelhante a insulina), MRFs (fatores regulatórios miogênicos), IL-6 (interleucina-6), VEGF (fator de crescimento vascular endotelial), Myf5 (fator miogênico 5), MRf4 (fator miogênico 4), MyoD (fator miogênico 1), androgênios e outros hormônios como a insulina. Todos estes fatores são dinâmicos e podem levar a uma maior compreensão da participação dessas células nos processos de regeneração tecidual. A interação com essas células promove ações positivas e negativas, levando a ativação ou inibição de suas atividades. O processo inflamatório e os fatores de crescimento atuam positivamente na diferenciação e proliferação celular. Entretanto, anti-inflamatórios, glicocorticoides e hormônios tireoidianos (T3) em excesso podem alterar o ciclo dessas células essenciais para o remodelamento tecidual. O entendimento de suas relações com as alterações fenotípicas e a sinalização que induzem modificações musculares é importante para o planejamento de estratégias de reabilitação e futuras terapias celulares.(AU)

The advance of molecular biology, cell biology and genetics has greatly improved our understanding of skeletal muscle biology, demonstrating that the biological characteristics of satellite cells (SCs) are essential in muscle regeneration. The therapeutic potential of these cells has been discussed in the literature, so the understanding of metabolism and regulation of precursor cells of the muscle fibers requires attention. The aim of this narrative review is to show aspects related to the regulatory factors of satellite cells during the process of skeletal muscle regeneration. Regeneration of skeletal muscle is an orchestrated process involving the activation and differentiation of SCs. The cellular interactions occur with various myogenic factors, growth factors and hormones. Stand out from HGF (Hepatocyte growth factor), IGF (insulin-like growth factor), MRFs (myogenic regulatory factors), IL-6 (interleukin-6), VEGF (vascular endothelial growth factor), Myf5 (myogenic factor 5), MRF4 (myogenic factor 4), MyoD (myogenic factor 1), androgens and other hormones such as insulin. All these factors are dynamic and can lead to a greater understanding of the role of cells in tissue regeneration processes. The interaction with these cells promotes positive and negative actions, leading to activation or inhibition of its activities. The inflammation and growth factors act positively on cell differentiation and proliferation. However, antiinflammatory, glucocorticoid and excess thyroid hormone (T3) may change the cycle of these cells essential to tissue remodeling. Understanding their relationship with the phenotypic changes and the signs inducing muscle changes is important for planning rehabilitation strategies and future cell therapies.(AU)