Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions.

KEY MESSAGE: Inheritance of the presence/absence of seeds in Annona squamosa is mediated by a single fully recessive gene and is caused by a deletion of the INNER NO OUTER (INO) locus. For some fruits, seedless varieties are desirable for consumption and processing. In the sugar apple tree (Annona squamosa L.), the seedless trait in the Thai seedless (Ts) and Brazilian seedless (Bs) accessions was associated with defective ovules and an apparent deletion of the INNER NO OUTER (INO) ovule development gene locus. Segregation analysis of F2 and backcross descendants of crosses of Bs to fertile wild-type varieties in this species with a multi-year generation time showed that seedlessness was recessive and controlled by a single locus. Comparison of whole genome sequence of a wild-type plant and a third accession, Hawaiian seedless (Hs), identified a 16 kilobase deletion including INO in this line. Ts and Bs lines were shown to have an identical deletion, indicating a common origin from a single deletion event. Analysis of microsatellite markers could not preclude the possibility that all three seedless accessions are vegetatively propagated clones. The sequence of the deletion site enabled a codominant assay for the wild-type and mutant genes allowing observation of complete cosegregation of the seedless/defective ovule phenotype with the INO deletion, showing maximal separation of less than 3.5 cM. The observed deletion is the only significant difference between the wild-type and Hs line over 587 kilobases, likely encompassing much more than 3.5 cM, showing that the deletion is the cause of seedless trait. The codominant markers and obtained progenies will be useful for introgression of the seedless trait into elite sugar apple lines and into other Annonas through interspecific crossings.

Growth and production of 'Prata Anã Gorutuba' banana under different planting densities

Increased planting density, different types of thinning management, and the use of beneficial microorganisms have been adopted as strategic tools in the effort to increase crop yields in a sustainable manner. This investigation evaluated the growth and production of ‘Prata Anã Gorutuba banana ( Musa spp., AAB) under different plant densities during two production cycles in the semiarid region of Minas Gerais, Brazil. Micropropagated plantlets were also inoculated with endophytic bacteria and cultivated as part of two mat management strategies. Planting density varied between 1,680 and 3,920 plant ha–1. During the experiment, water was less than optimal because of rationing due to drought in the region. Under field conditions, there was no difference between bacterially inoculated and non-inoculated plants. The dense planting system increased the crop cycle. The highest-density planting treatment (3,920 plant ha–1) resulted in the highest yield in the first cycle, but no net income was observed. However, the high-density planting treatments resulted in the highest yields and gross incomes in the second production cycle. Management with thinning of plants up to 10-months-old and thinning up to harvest associated with high planting density increased the length of the production cycles. Although the water restriction experienced in the first and second crop cycles, the vegetative and productive traits of ‘Prata Anã Gorutuba banana did not compromise the objectives of this study.(AU)

Growth and production of 'Prata Anã Gorutuba' banana under different planting densities

Increased planting density, different types of thinning management, and the use of beneficial microorganisms have been adopted as strategic tools in the effort to increase crop yields in a sustainable manner. This investigation evaluated the growth and production of ‘Prata Anã Gorutuba’ banana ( Musa spp., AAB) under different plant densities during two production cycles in the semiarid region of Minas Gerais, Brazil. Micropropagated plantlets were also inoculated with endophytic bacteria and cultivated as part of two mat management strategies. Planting density varied between 1,680 and 3,920 plant ha–1. During the experiment, water was less than optimal because of rationing due to drought in the region. Under field conditions, there was no difference between bacterially inoculated and non-inoculated plants. The dense planting system increased the crop cycle. The highest-density planting treatment (3,920 plant ha–1) resulted in the highest yield in the first cycle, but no net income was observed. However, the high-density planting treatments resulted in the highest yields and gross incomes in the second production cycle. Management with thinning of plants up to 10-months-old and thinning up to harvest associated with high planting density increased the length of the production cycles. Although the water restriction experienced in the first and second crop cycles, the vegetative and productive traits of ‘Prata Anã Gorutuba’ banana did not compromise the objectives of this study.

Atemoya fruit development and cytological aspects of GA3-induced growth and parthenocarpy.

The exogenous application of GA3 to atemoya tree flowers induces parthenocarpy, and in association with artificial pollination, it increases the fruit size. Morphological, anatomical, ultrastructural, and chemical aspects were evaluated during development of (1) fruit produced by artificial pollination (AP), (2) fruit from AP followed by the application of 250 ppm GA3, and (3) parthenocarpic fruit induced by the application of 1000 ppm GA3. Fruit growth showed a sigmoidal pattern, with development occurring in three phases: (I) cell division, (II) cell differentiation, and (III) maturation. Phase I presented cells with large nuclear volumes and a large population of organelles, phase II presented cells with a reduction in cytoplasm and an increase in vacuole volume, and phase III presented cells with an increase in plastids with reserve compounds. The application of GA3, in association with pollination, precedes cytological events and delays when applied exclusively. GA3 promotes the growth of pollinated fruits by stimulating cell division and expansion, which occur in association with reduced seed production, and the GA3 induces parthenocarpy by maintaining division and stimulating cell expansion. The absence of seeds accounts for the smaller size of the parthenocarpic fruits, and the lower accumulation of calcium accounts for less firm fruit.

Gibberellic acid combined with hand pollination increases 'Red' and 'Lessard Thai' sugar apple fruit quality and produced parthenocarpic 'Gefner' atemoya fruits / Ácido giberélico associado com a polinização manual incrementa a qualidade dos frutos em anonáceas e produz frutos partenocárpicos em atemoieira 'Gefner'

ABSTRACT: The present research aimed to evaluate the effect of applying gibberellic acid (GA3) with hand (HP) or natural pollination (NP) on fruit set and the fruit quality of 'Red' and 'Lessard Thai' sugar apple and 'Gefner' atemoya fruits.This study was performed in an experimental orchard located in Homestead, Florida, USA. The experimental design included randomized blocks, with eight treatments, four replicates and four plants per plot. Treatments included: (1) HP; (2) HP + 10 mg L-1 GA3; (3) HP + 100 mg L-1 GA3; (4) HP + 1,000 mg L-1 GA3; (5) NP; (6) NP + 10 mg L-1 GA3; (7) NP + 100 mg L-1 GA3 and (8) NP + 1,000 mg L-1 GA3. The HP plus 1,000 mg L-1 GA3 promoted fruit setting above 90% over the 14 weeks for all genotypes evaluated. Significant increments for length and total fruit weight were observed. 'Red' sugar apple and atemoya had a reduced number of seeds per fruit. The NP plus GA3 (1,000 mg L-1) was effective in producing high quality seedless 'Gefner' atemoya fruits. This investigationdemonstratedthat GA3 plus hand pollination produced high quality sugar apple and atemoya seeded fruits and in association with natural pollination promoted seedless 'Gefner'atemoya fruits.

RESUMO: O presente trabalho teve como objetivo avaliar o efeito da aplicação de ácido giberélico (GA3) associado com e sem polinização artificial, no pegamento e na qualidade de frutos das pinheiras 'Red' e 'Lessard Thai' e da atemoieira'Gefner'. O experimento foi realizado em pomar localizado em Homestead, EUA. O delineamento experimental foi em blocos casualizados, com oito tratamentos, quatro repetições e quatro plantas por parcela. Os tratamentos utilizados neste experimento foram: 1) Polinização Artificial (PA); 2) PA + 10 mg L-1 GA3; 3) PA + 100 mg L-1 GA3; 4) PA + 1000 mg L-1 GA3; 5) Polinização natural (PN); 6) PN + 10 mg L-1 GA3; 7) PN + 100 mg L-1 GA3; e 8) PN + 1000 mg L-1 GA3. A PA combinada com a concentração de 1000 mg L-1 de GA3 promoveu o estabelecimento de frutos acima de 90% durante as 14 semanas de avaliação para todos os genótipos avaliados. Incrementos significativos para o comprimento e peso dos frutos também foram observados a variedade de pinha "Red" e a atemoia, em que apresentaram redução no número total de sementes.A PN em associação com GA3 (1000 mg L-1) foi eficaz na produção de frutos sem sementes de alta qualidade em atemóia 'Gefner. Estes dados mostram que o GA3, combinado com a polinização manual, produziu frutos com semente de alta qualidade e, em associação com a polinização natural, promoveu frutos sem sementes em atemóia 'Gefner'.

Gibberellic acid combined with hand pollination increases 'Red' and 'Lessard Thai' sugar apple fruit quality and produced parthenocarpic 'Gefner' atemoya fruits / Ácido giberélico associado com a polinização manual incrementa a qualidade dos frutos em anonáceas e produz frutos partenocárpicos em atemoieira 'Gefner'

The present research aimed to evaluate the effect of applying gibberellic acid (GA3) with hand (HP) or natural pollination (NP) on fruit set and the fruit quality of ‘Red and ‘Lessard Thai sugar apple and ‘Gefner atemoya fruits.This study was performed in an experimental orchard located in Homestead, Florida, USA. The experimental design included randomized blocks, with eight treatments, four replicates and four plants per plot. Treatments included: (1) HP; (2) HP + 10 mg L-1 GA3; (3) HP + 100 mg L-1 GA3; (4) HP + 1,000 mg L-1 GA3; (5) NP; (6) NP + 10 mg L-1 GA3; (7) NP + 100 mg L-1 GA3 and (8) NP + 1,000 mg L-1 GA3. The HP plus 1,000 mg L-1 GA3 promoted fruit setting above 90% over the 14 weeks for all genotypes evaluated. Significant increments for length and total fruit weight were observed. ‘Red sugar apple and atemoya had a reduced number of seeds per fruit. The NP plus GA3 (1,000 mg L-1) was effective in producing high quality seedless ‘Gefner atemoya fruits. This investigation demonstrated that GA3 plus hand pollination produced high quality sugar apple and atemoya seeded fruits and in association with natural pollination promoted seedless ‘Gefneratemoya fruits.(AU)

O presente trabalho teve como objetivo avaliar o efeito da aplicação de ácido giberélico (GA3) associado com e sem polinização artificial, no pegamento e na qualidade de frutos das pinheiras ‘Red e ‘Lessard Thai e da atemoieira‘Gefner. O experimento foi realizado em pomar localizado em Homestead, EUA. O delineamento experimental foi em blocos casualizados, com oito tratamentos, quatro repetições e quatro plantas por parcela. Os tratamentos utilizados neste experimento foram: 1) Polinização Artificial (PA); 2) PA + 10 mg L-1 GA3; 3) PA + 100 mg L-1 GA3; 4) PA + 1000 mg L-1 GA3; 5) Polinização natural (PN); 6) PN + 10 mg L-1 GA3; 7) PN + 100 mg L-1 GA3; e 8) PN + 1000 mg L-1 GA3. A PA combinada com a concentração de 1000 mg L-1 de GA3 promoveu o estabelecimento de frutos acima de 90% durante as 14 semanas de avaliação para todos os genótipos avaliados. Incrementos significativos para o comprimento e peso dos frutos também foram observados a variedade de pinha “Red” e a atemoia, em que apresentaram redução no número total de sementes.A PN em associação com GA3 (1000 mg L-1) foi eficaz na produção de frutos sem sementes de alta qualidade em atemóia ‘Gefner. Estes dados mostram que o GA3, combinado com a polinização manual, produziu frutos com semente de alta qualidade e, em associação com a polinização natural, promoveu frutos sem sementes em atemóia ‘Gefner.(AU)

Floral induction management in 'Palmer' mango using uniconazole / Manejo da indução floral da mangueira 'Palmer' com uso de uniconazole

ABSTRACT: The objective of this study was to evaluate the effects of different doses of uniconazole (UCZ) on 'Palmer' mango vegetative and reproductive response and on fruit physical, chemical, and productive characteristics during the off-season in northern Minas Gerais, Brazil. The experiment followed a randomized block design. UCZ was tested at the following doses applied to leaves and expressed in g of UCZ active ingredient (a.i.) per tree: 0.0, 1.0, and 1.0+1.0 (2.0) after 30 days; 1.0+1.0+1.0 (3.0) subdivided into 30-day intervals; and 1.0+1.0+2.0 (4.0) subdivided into 30-day intervals. All of the UCZ treatments reduced branch elongation in the 'Palmer' mango trees, leading to a mean reduction of 81.6% compared to the control. However, the UCZ application of 1.0+1.0+2.0g a.i. per tree subdivided into 30-day intervals was efficient in promoting flowering during the off-season, enabling a 167% mean increase in the number of fruit per tree and a 9.78t ha-1 mean increase in productivity.

RESUMO: Este trabalho teve como objetivo avaliar a eficiência da aplicação de diferentes doses de uniconazole (UCZ) em mangueira 'Palmer' no comportamento vegetativo, reprodutivo e nas características físicas, químicas e produtivas dos frutos durante o período de entressafra na região Norte de Minas Gerais. O delineamento experimental foi em blocos ao acaso, testando-se as doses 0,0; 1,0; 1,0+1,0 (2,0) após 30 dias; 1,0+1,0+1,0 (3,0) parcelado a cada 30 dias; 1,0+1,0+2,0 (4,0) parcelado a cada 30 dias, em g, do ingrediente ativo (i.a.) de UCZ por planta, aplicado via foliar. Verificou-se que todos os tratamentos com uso de uniconazole reduziram o alongamento dos ramos da mangueira 'Palmer', quando comparada com a testemunha, apresentando uma redução média de 81,6%, no entanto, apenas a aplicação de 1,0+1,0+2,0g i.a. UCZ/planta parcelado a cada 30 dias foi eficiente em promover a floração no período da entressafra, possibilitando um incremento médio de 167% no número de frutos por planta e de 9,78t ha-1 na produtividade.

Floral induction management in 'Palmer' mango using uniconazole / Manejo da indução floral da mangueira 'Palmer' com uso de uniconazole

The objective of this study was to evaluate the effects of different doses of uniconazole (UCZ) on 'Palmer' mango vegetative and reproductive response and on fruit physical, chemical, and productive characteristics during the off-season in northern Minas Gerais, Brazil. The experiment followed a randomized block design. UCZ was tested at the following doses applied to leaves and expressed in g of UCZ active ingredient (a.i.) per tree: 0.0, 1.0, and 1.0+1.0 (2.0) after 30 days; 1.0+1.0+1.0 (3.0) subdivided into 30-day intervals; and 1.0+1.0+2.0 (4.0) subdivided into 30-day intervals. All of the UCZ treatments reduced branch elongation in the 'Palmer' mango trees, leading to a mean reduction of 81.6% compared to the control. However, the UCZ application of 1.0+1.0+2.0g a.i. per tree subdivided into 30-day intervals was efficient in promoting flowering during the off-season, enabling a 167% mean increase in the number of fruit per tree and a 9.78t ha-1 mean increase in productivity.(AU)

Este trabalho teve como objetivo avaliar a eficiência da aplicação de diferentes doses de uniconazole (UCZ) em mangueira 'Palmer' no comportamento vegetativo, reprodutivo e nas características físicas, químicas e produtivas dos frutos durante o período de entressafra na região Norte de Minas Gerais. O delineamento experimental foi em blocos ao acaso, testando-se as doses 0,0; 1,0; 1,0+1,0 (2,0) após 30 dias; 1,0+1,0+1,0 (3,0) parcelado a cada 30 dias; 1,0+1,0+2,0 (4,0) parcelado a cada 30 dias, em g, do ingrediente ativo (i.a.) de UCZ por planta, aplicado via foliar. Verificou-se que todos os tratamentos com uso de uniconazole reduziram o alongamento dos ramos da mangueira 'Palmer', quando comparada com a testemunha, apresentando uma redução média de 81,6%, no entanto, apenas a aplicação de 1,0+1,0+2,0g i.a. UCZ/planta parcelado a cada 30 dias foi eficiente em promover a floração no período da entressafra, possibilitando um incremento médio de 167% no número de frutos por planta e de 9,78t ha-1 na produtividade.(AU)

Aplicação em pré-colheita de cloreto de cálcio no controle do despencamento natural dos frutos de bananeira 'FHIA-18' / Calcium chloride in preharvest application to control the natural fruit dropping of 'FHIA-18' banana tree

RESUMO:O objetivo deste trabalho foi avaliar o efeito da aplicação de CaCl2 em pré-colheita, em diferentes doses e épocas no controle do despencamento natural de frutos da bananeira 'FHIA-18'. O delineamento experimental foi o de blocos casualizados, em esquema fatorial 3x3+1 testemunha, sendo testadas as doses de 2, 4 e 6% de CaCl2 aos 60, 90 e 120 dias após a emissão do cacho, com cinco repetições e uma planta por parcela. Observou-se redução linear da resistência ao despencamento e do tempo de maturação do fruto com o acréscimo das doses de CaCl2. Os frutos apresentaram maior firmeza com três aplicações de CaCl2. Os teores de cálcio da casca e da polpa não foram alterados significativamente com as doses e épocas de aplicações do CaCl2, indicando que o cálcio não é absorvido efetivamente pelo fruto. A aplicação de CaCl2na pré-colheita não reduz o despencamento dos frutos da bananeira 'FHIA-18'.

ABSTRACT:The objective of this research was to evaluate the effect of CaCl2 application in preharvest and in different doses and times in order to control the natural fruit dropping of banana 'FHIA-18'. The experimental design was in a randomized blocks, in a factorial scheme 3x3+1 control, with three doses of CaCl2 (2, 4 and 6%), three times of application (60, 90 and 120 days) after the bunch emission, with five replicates and one plant per parcel. A linear decrease of dropping resistance and fruit time maturation with increasing doses of CaCl2 was observed. The fruits showed to be more consistent with three applications of CaCl2. The calcium content of the peel and pulp were not significantly changed with the doses and applications of CaCl2, indicating that calcium is not absorbed effectively by the fruits. The application of CaCl2 in preharvest do not reduce the natural fruit dropping in banana 'FHIA-18'.

Aplicação em pré-colheita de cloreto de cálcio no controle do despencamento natural dos frutos de bananeira FHIA-18 / Calcium chloride in preharvest application to control the natural fruit dropping of FHIA-18 banana tree

O objetivo deste trabalho foi avaliar o efeito da aplicação de CaCl2 em pré-colheita, em diferentes doses e épocas no controle do despencamento natural de frutos da bananeira 'FHIA-18'. O delineamento experimental foi o de blocos casualizados, em esquema fatorial 3x3+1 testemunha, sendo testadas as doses de 2, 4 e 6% de CaCl2 aos 60, 90 e 120 dias após a emissão do cacho, com cinco repetições e uma planta por parcela. Observou-se redução linear da resistência ao despencamento e do tempo de maturação do fruto com o acréscimo das doses de CaCl2. Os frutos apresentaram maior firmeza com três aplicações de CaCl2. Os teores de cálcio da casca e da polpa não foram alterados significativamente com as doses e épocas de aplicações do CaCl2, indicando que o cálcio não é absorvido efetivamente pelo fruto. A aplicação de CaCl2 na pré-colheita não reduz o despencamento dos frutos da bananeira 'FHIA-18'.(AU)

The objective of this research was to evaluate the effect of CaCl2 application in preharvest and in different doses and times in order to control the natural fruit dropping of banana 'FHIA-18'. The experimental design was in a randomized blocks, in a factorial scheme 3x3+1 control, with three doses of CaCl2 (2, 4 and 6%), three times of application (60, 90 and 120 days) after the bunch emission, with five replicates and one plant per parcel. A linear decrease of dropping resistance and fruit time maturation with increasing doses of CaCl2 was observed. The fruits showed to be more consistent with three applications of CaCl2. The calcium content of the peel and pulp were not significantly changed with the doses and applications of CaCl2, indicating that calcium is not absorbed effectively by the fruits. The application of CaCl2 in preharvest do not reduce the natural fruit dropping in banana 'FHIA-18'.(AU)

Analysis of the abilities of endophytic bacteria associated with banana tree roots to promote plant growth.

A total of 40 endophytic bacterial isolates obtained from banana tree roots were characterized for their biotechnological potential for promoting banana tree growth. All isolates had at least one positive feature. Twenty isolates were likely diazotrophs and formed pellicles in nitrogen-free culture medium, and 67% of these isolates belonged to the genus Bacillus sp. The isolates EB-04, EB-169, EB-64, and EB-144 had N fixation abilities as measured by the Kjeldahl method and by an acetylene reduction activity assay. Among the 40 isolates, 37.5% were capable of solubilizing inorganic phosphate and the isolates EB-47 and EB-64 showed the highest solubilization capacity. The isolate EB-53 (Lysinibacillus sp.) had a high solubilization index, whereas 73% of the isolates had low solubilization indices. The synthesis of indole-3-acetic acid (IAA) in the presence of L-tryptophan was detected in 40% of the isolates. The isolate EB-40 (Bacillus sp.) produced the highest amount of IAA (47.88 µg/ml) in medium supplemented with L-tryptophan and was able to synthesize IAA in the absence of L-tryptophan. The isolates EB-126 (Bacillus subtilis) and EB-47 (Bacillus sp.) were able to simultaneously fix nitrogen, solubilize phosphate and produce IAA in vitro. The results of this study demonstrated that the isolates analyzed here had diverse abilities and all have the potential to be used as growth-promoting microbial inoculants for banana trees.

Crescimento, produção e qualidade de frutos de atemoieira ‘Gefner’ submetida a diferentes intensidades de poda / Growth, yield and fruit quality of atemoya trees ‘Gefner’ under different pruning intensities

Incrementos na produtividade e na qualidade dos frutos de atemoieira, principalmente no período de entressafra, são prioridades dos produtores. O presente trabalho teve como objetivo avaliar o crescimento e a produção da atemoieira 'Gefner' submetida a diferentes intensidades de poda. O delineamento experimental foi o de blocos casualizados com cinco tratamentos, quatro repetições e uma planta por parcela. Os tratamentos utilizados foram ramos podados com 10, 20, 30, 40 e 50cm de comprimento. Foram avaliados o número de flores, comprimento e diâmetro dos ramos brotados, diâmetro da copa e altura da planta, número de frutos na pré-colheita e colheita, peso total de frutos, peso médio de frutos, diâmetro dos frutos, comprimento dos frutos, peso da casca dos frutos, peso de polpa e semente dos frutos, teor de sólidos solúveis, acidez titulável e pH. As características avaliadas foram submetidas à análise de variância, tendo os efeitos dos diferentes comprimentos dos ramos podados testados e ajustados em equações de regressão. Não foram observados efeitos significativos sobre as características altura de plantas, diâmetro da copa, peso total de frutos, diâmetro dos frutos, comprimento dos frutos, peso da casca dos frutos, peso de polpa e semente dos frutos, teor de sólidos solúveis, acidez titulável e pH. Entretanto, foi observada redução linear do número de flores, comprimento e diâmetro dos ramos brotados, à medida que aumentou a intensidade de poda.

Improvements in yield and fruit quality in atemoya, mainly during the period of out of season are priorities for growers. The purpose of this study was to evaluate the growth and yield of atemoya 'Gefner' under different pruning intensities. The experimental design was randomized blocks, with five treatments, four replications and one plant per block. The treatments were pruned to 10, 20, 30, 40 and 50cm in length. Number of flowers, length and diameter of branches sprouted, crown diameter, plant height, number of fruits in pre-harvest and harvest periods, total weight of fruits, fruit weight, fruit diameter, fruit length, peel, pulp and seed weight, soluble solids, titratable acidity and pH were evaluated. The characteristics were submitted to analysis of variance, and the effects of different lengths of branches prunedtested and adjusted in the regression equation. No significant influence were observed fordiameter of the crown, plant height, number of fruit in pre-harvest and harvest periods, totalweight of fruits, fruit weight, fruit diameter, fruit length, peel, pulp and seed weight, solublesolids, titratable acidity and pH. However, as the increased pruning intensities showed a linear reduction in the number of flowers, length and diameter of branches sprouted in atemoya tree 'Gefner'.

Avaliação fitotecnica de bananeiras tipo terra sob irrigação em condições semi-áridas / Agronomical evaluation of bananas under irrigation and semi-arid conditions

Conduziu-se este trabalho, com o objetivo de avaliar fitotecnicamente as características vegetativas e de rendimento de cinco cultivares de bananeira tipo Terra, em primeiro ciclo de produção sob irrigação nas condições semiáridas da região de Guanambi - BA. Utilizaram-se descritores fenotípicos relevantes para a identificação e seleção de indivíduos com características superiores, visando a disponibilizar aos produtores dos perímetros de irrigação uma alternativa para a diversificação da bananicultura. Avaliaram-se as seguintes características: número de dias do plantio ao florescimento e à colheita, altura da planta, perímetro do pseudocaule, número de folhas vivas nas épocas do florescimento e colheita, o intervalo florescimento-colheita, peso do cacho, da ráquis e das pencas, número de pencas e de frutos, comprimento e diâmetro do fruto. O experimento foi delineado em blocos casualizados, com cinco tratamentos, constituídos pelas cultivares AAB: Terra, Terra-Maranhão, Terrinha e D'Angola e pelo híbrido AAAB: FHIA-21 com cinco repetições. Cada parcela foi constituída por 20 plantas (quatro fileiras de cinco plantas), com seis plantas úteis no espaçamento de 3,0 m x 3,0 m. Destacaram-se as cultivares avaliadas a Terra e a Terra Maranhão como as mais produtivas, porém, com maior altura e mais tardias em ciclo. O híbrido FHIA-21 e as cultivares Terrinha e D'Angola, apresentaram-se com maior número de folhas vivas à época da colheita. A cultivar D'Angola apresentou a menor produtividade dentre as cultivares avaliadas.

The goal of this work was to make a agronomical evaluation of the vegetative characteristics and income of five cultivars of banana type Plantain, in the first production cycle under irrigation in the semi-arid conditions of the region of the city of Guanambi -Bahia State. Relevant phenotypic descriptors were used for the identification and selection of individuals with superior characteristics, so as to make available to the producers of the perimeters of irrigation an alternative for the diversification of the banana crops. The evaluated genotypes AAB were the varieties Terra, Terra-Maranhão, Terrinha and D'Angola and the hybrid AAAB: FHIA-21. The following characteristics were analyzed: number of days from the sowing to the flowering and to the harvest, height of the plant, perimeter of the pseudostem, number of alive leaves at flowering and harvest, gap flowering-harvest, weight of the bunch, of the peduncle and of the hands, number of hands and fruits per bunch, length and diameter of the fruit. The procedure employed was randomized blocks, with five treatments, consisting of the cultivars AAB: Terra, Terra-Maranhão, Terrinha and D'Angola and the hybrid AAAB: FHIA-21, and five repetitions. Each plot was comprised by 20 plants: four rows of five plants, with six useful plants in the spacing of 3,0 m x 3,0 m. Terra and Terra Maranhão were the most productive varieties, however, with larger load and late cycles. The hybrid FHIA-21 and the varieties Terrinha and D'Angola showed a larger number of living leaves in the harvest. The variety D'Angola presented the smallest productivity between the evaluated cultivars.

Viabilidade dos grãos de pólen de flores de pinheira (Annona squamosa) em diferentes horários / Viability of the sugar apple (Annona squamosa) pollen grains at different hours of the day

No manejo do cultivo da pinha (Annona squamosa), a polinização artificial é uma prática preconizada para, obter maior pegamento dos frutos bem como uniformização do formato dos mesmos. Nesse sentido, conduziu-se este trabalho, com o objetivo de avaliar a viabilidade dos grãos de pólen de flores de pinheira em diferentes horários de coleta. O pólen foi obtido a partir de flores no estádio funcionalmente estaminada. Foram avaliados oito horários de coleta de pólen: zero hora, 1 hora, 2 horas, 3 horas, 4 horas, 5 horas, 6 horas e 7 horas da manhã. Foi utilizado meio de cultura padrão para germinação de pólen, com concentração de 10% de sacarose. As flores foram coletadas nos horários estabelecidos e os grãos de pólen foram retirados das anteras com auxílio de um pincel número 2 e em seguida inoculados em placas de Petri contendo o meio de cultura. O delineamento experimental foi inteiramente casualizado com quatro repetições, sendo cada parcela experimental constituída por duas placas Petri. Foram contados 100 grãos de pólen por placa. Após 6 horas de inoculação, os grãos de pólen foram visualizados sob lupa. Foram considerados germinados os grãos de pólen que possuíam tubo polínico com tamanho igual ou superior ao diâmetro do próprio pólen. Não foram observadas diferenças significativas entre o horário de coleta dos grãos de pólen. A percentagem média da germinação variou de 46,75% a 53,62% dos grãos de pólen germinados.

In the management of the sugar apple (Annona squamosa) crop, the artificial pollination is a preconized practice to obtain higher establishment of the fruits as well as their standardization. This study was carried out to evaluate the viability of sugar apple pollen grains at different collecting times. The pollens were obtained from the flowers at the functional staminate stage. The pollen grains were collected every hour, starting from 00:00am and ending at 07:00am, totalizing 8 collections. The standard culture medium with 10 percent sucrose was used for the germination of the pollen. The flowers were collected at the scheduled hours and the pollen grains were taken from the anthers, by using a brush # 2. They were then inoculated on Petri dishes containing the culture medium. The completely randomized experimental design was used with four replicates, and each plot was constituted by two Petri dishes. One hundred pollen grains were counted in each Petri dish. After six hours of inoculation, the pollen grains were visualized under magnifying glass. Those pollen grains containing the pollinic tube with the same or higher size than their own pollen diameter were considered germinated. No significant differences were found among the different hours of the pollen grain collections, and the germination percentage ranged from 46.75% to 53.62%.

Estabelecimento in vitro de explantes de três cultivares de bananeira / Explants establishment in vitro of three banana cultivars

O objetivo do presente trabalho foi avaliar dois protocolos de desinfestação na micropropagação em três cultivares de bananeira. Utilizaram-se mudas das cultivares de bananeira Prata Anã, FHIA 18 e SH3640. Os protocolos 1 e 2 de desinfestação (Des. 1 e Des. 2) foram realizados utilizando-se os seguintes produtos: solução fungicida de Derosol, álcool comercial, solução de hipoclorito de sódio e de hipoclorito de cálcio e tween 20, apresentando variações na concentração dos produtos em cada um dos protocolos. As avaliações para a porcentagem de contaminação foram realizadas diariamente por meio do número total de tubos contaminados e não contaminados. O delineamento foi em blocos casualizados, com quatro repetições, em um sistema fatorial. As contaminações que ocorreram foram causadas exclusivamente por bactérias. Independentemente do tratamento utilizado, os índices de contaminação foram superiores a 29 por cento para as três cultivares testadas. Estes resultados indicam a necessidade de adequar novas metodologias de assepsias de explantes de bananeira.

The purpose of this work was to evaluate two protocols of disinfestations in the micropropagation on young plants of three banana cultivars: Prata-Anã, FHIA 18 and SH3640. Protocols 1 and 2 of disinfestations (Dis. 1 and Dis. 2) were carried out using the products: fungicidal solution of Derosol, commercial alcohol, solution of sodium hypo chlorite and calcium hypo chlorite and tween 20, presenting variations in their concentration in every one of the protocols. The evaluations for contamination rate were taken daily by means of the total number of contaminated and non-contaminated tubes. A random block design in a factorial scheme with four repetitions was used. Exclusively bacteria had caused all contamination. Independently of the used treatment the contamination levels had been up to 29 percent for all the three cultivars tested. These results indicate the necessity of adjusting the new methodologies of asepses of banana explants.