Hair cortisol and dehydroepiandrosterone sulfate concentrations in healthy beef calves from birth to 6 months of age.

Cortisol (C) and dehydroepiandrosterone (DHEA) are recognized as the main fetal steroids, and they are likely to influence fetal development and have long-term effects on newborn hypothalamic-pituitary-adrenal axis (HPA) function. DHEA is often measured as its sulfates and expressed as DHEA-S. Hair analysis represents a promising methodological approach for the non-invasive measurement of steroids, allowing for a retrospective analysis of the total exposure to steroids over time, and avoiding the influence of acute events or circadian fluctuations. Hair cortisol and DHEA concentrations have been investigated in cows, but no studies have been performed on calves. The object of this study was to evaluate hair cortisol (HC) and hair DHEA-S (HDHEA-S) concentrations in beef calves from birth to six months of age. Hair samples of 12 beef calves (seven males, five females) were firstly collected at birth (T1) and then every three weeks up to six months of age (T2-T10), collecting only the re-grown hair. HC and HDHEA-S were analyzed by radioimmunoassay (RIA). Calves sex, weight and APGAR score were registered immediately after birth. Statistical analysis revealed that both HC and HDHEA-S were influenced by sampling time (P < 0.001). HC concentrations were higher at T1 compared to all subsequent samplings (T2-T10, P < 0.01); HC concentrations were higher at T2 compared to T4-T10 (P < 0.01), while no further changes were detected from T3 onward. Higher HDHEA-S concentrations were registered at T1, T2 and T3 compared to all the other samplings (P < 0.01). No correlation was found between hair concentrations of both steroids and calf sex or birthweight. APGAR score was negatively correlated only with HC at birth (P < 0.05). These data demonstrate that C and DHEA-S are quantifiable in the hair of calves and are influenced by their age. The higher HC detected at birth (T1) probably reflects the high serum C concentrations present late in pregnancy and increased by the fetal HPA axis, by which parturition is initiated in cows. The highest HDHEA-S at birth (T1) in calves indicates that the largest amounts of DHEA and its sulfates are produced during fetal development. Moreover, the findings of higher HC at three weeks after birth and of higher HDHEA-S until six weeks after birth, suggest that C and DHEA secretion continues also beyond birth, and that these steroids could be involved in the events occurring during the challenging first weeks of age in the calf.

Comparison of AlphaLISA and RIA assays for measurement of wool cortisol concentrations.

Radioimmunoassay (RIA) methods have always represented a technique of choice for the determination of steroids in biological samples. The Amplified Luminescent Proximity Homogenous Assay-Linked Immunosorbent Assay (AlphaLISA) is now emerging as the new-generation immunoassay technology that does not require washing/separation steps. The aim of this study was to adapt the Perkin-Elmer's AlphaLISA kit for wool cortisol and compare it with a RIA wool cortisol assay. Wool from lambs, 35 at birth (A0) and 54 at two months old (A2), was collected and each extract was evaluated for wool cortisol concentrations (HCC) both by RIA and AlphaLISA immunoassay. The two methods showed good precision, sensitivity and specificity for determining HCC. Both methods were able to detect significant differences between the high and the low HCC assessed in lambs at A0 and A2 (P < 0.01). The HCC assessed with RIA were significantly higher than those assessed with AlphaLISA (P < 0.01). Moreover, the correlation between HCC measured using the AlphaLISA and RIA methods was strong (r = 0.878). The regression analyses show a constant and not proportional error. This could be due to the diversity in the dosage steps and to the diversity of the molecules used in the two methods. Results support the validity of using AlphaLISA as an alternative method to RIA for the quantification of cortisol in sheep wool and considering the performances showed it has a great potential to be further applied as an excellent tool to evaluate HCC in samples derived from animal species.

Luteal activity and effect of dietary energy restriction on follicular development in lactating cows.

The aim of this research has been to evaluate the presence of anomalies in the ovarian cycle activity during postpartum and to verify whether 72-hr dietary fasting during the dominance phase, the phase before ovulation, might modify the ovarian follicle population. The presence of anomalies in ovarian cycle activity has been evaluated in 30 Italian Friesian cows starting from 20 days postpartum until 211 days of lactation. Long oestrus and brief dioestrus or scarce luteal activity have been the main anomalies found through measuring progesterone concentrations in the whey. Until 100 days of lactation, the BCS values of the problematic animals have been significantly lower than those in animals with normal ovarian activity. After 100 days of lactation, the ovarian anomalies continued to appear despite the fact that all the animals have reached comparable BCS values. Starting from the results of this trial, the effect of 72-hr dietary fasting on dominant follicles has been studied in six cows. Ultrasonography revealed that the diameter of the follicles at 71 days postpartum has been significantly lower than at 181 days. A 72-hr dietary restriction at 101 and 211 days postpartum did not affect the size of the dominant follicle. However, at 101 days postpartum, half of the animals presented follicular cysts. The effect of fasting differed if the animal has been in early postpartum or 211 days of lactation. Further researches are necessary to understand how different metabolic conditions can modify the follicular population but on the other hand the study shows the utility for farmers and field veterinarians of monitoring the resumption of the ovarian cycle postpartum through the whey progesterone concentrations.

Study of progesterone and cortisol concentrations in the Italian Friesian claw.

The present research was conducted to study progesterone and cortisol concentrations in the claw of cattle and to verify whether the cattle claw could be considered an efficient matrix to provide retrospective information regarding progesterone and cortisol concentrations related to pregnancy and peripartum periods. These 2 steroids are involved in hoof growth. The study was performed on 32 calves and 24 pregnant milking cows of the Holstein breed, which were clinically healthy and lacking any claw disorders. Samples of at least 0.5cm in thickness were taken from the sole. Progesterone and cortisol concentrations were determined by RIA. The cortisol concentration in the horny shoe of calves from 0 to 30 d of age was significantly higher than the concentration at 31 to 60 and 61 to 120 d of age. Conversely, the progesterone concentration showed no statistically significant difference in relation to age. The horn progesterone concentrations recorded in the milking dairy cows at 7 mo of pregnancy showed high variability in the horizontal sections of the sole (the individual coefficient of variation ranged between 0.09 and 1.11). In 6 cows, genuine extreme values (genuine outliers) of the progesterone level were found. Moreover, significant differences existed among the progesterone concentrations of the sole's transverse sections. We detected a significant positive correlation between the weight of the horn samples after freeze-drying and their weight after hydration. The cortisol and progesterone levels in soaked horn samples were found to be significantly lower than in the same dry samples. These results show that cortisol and progesterone can be measured in the cattle claw horn. The claws of mature dairy cows could not be used as a matrix to provide a retrospective measure of cumulative hormone secretion, whereas the analysis of the calves' claw horns showed retrospective hormonal information similar to hair samples.

Short communication: hair cortisol concentrations in Holstein-Friesian and crossbreed F1 heifers.

The aim of this study was to evaluate the hypothalamic-pituitary-adrenal (HPA) axis activity of Holstein-Friesian and crossbreed F1 heifers by analysis of the cortisol concentrations in hair samples. Cortisol, the primary hormone of the HPA axis, is the biological endpoint for the investigation of the HPA response. The study was conducted on 290 prepubertal heifers; 142 heifers were pure Holstein-Friesian and 148 were crossbreed F1 heifers obtained from the 3-way rotational system with Swedish Red and Montbéliarde breeds. Extraction was performed on the hair using methanol, and cortisol concentrations were determined by a radioimmunoassay method. Cortisol concentrations measured in regrown hair of crossbreed F1 heifers were significantly lower than those in hair of Holstein-Friesian heifers. This result helps us to better understand the differences in HPA activity and allostatic load between Holstein-Friesian and crossbreed F1 heifers and allows us to better assess the adaptability of these animals to the environment and the importance of crossbreed traits for profitability in dairy farming.

Case series: clinical findings and oral rehabilitation of patients with amelogenesis imperfecta.

BACKGROUND: Children with amelogenesis imperfecta (AI) experience many oral difficulties including sensitivity and aesthetics. The methods of treating AI children are limited and therefore a program of care was evaluated in order to assess the clinical efficacy of providing preventive and restorative treatments. CASE REPORTS: A non-randomised convenience sample of 12 patients with AI was evaluated. A comprehensive patient history was recorded, followed by a clinical and radiographic assessment of oral health. In 8/12 patients a hypoplastic form of AI was diagnosed, in 2/12 cases hypomaturation and in 2/12 cases hypocalcified form were noted. Chief complaints were mainly related to unsatisfactory aesthetics and dental sensitivity. In 8 patients there was active dental caries. Most of the patients had gingivitis and showed fair oral hygiene. The presence of non-enamel dental anomalies was recorded in 9 patients. TREATMENT: All patients received meticulous preventive care. Initial treatment depended on AI type and oral health of the patient. During the transition period, both conventional and resin modified glassionomer cements, as well as composite resin materials, were used to restore posterior teeth. Direct composite resin restorations were used to improve the appearance of anterior teeth. In 4 patients a long-lasting interdisciplinary approach including orthodontics, metal-ceramic crowns and fixed partial dentures, and direct composite restorations was required. FOLLOW-UP: Follow-up periods varied between 2-11 years. All children have been regularly recalled at 3 monthly intervals. Caries prevalence has remained low during the follow-up postoperative period and patients have reported satisfaction with the treatment they have received. CONCLUSION: AI is associated with multiple non-enamel anomalies and requires a complex treatment. Treatment planning is related to the age of the patient, the type and severity of the disorder, and the oral health of the patient. Early diagnosis, preventive care and timely treatment are of foremost importance to improve oral health in children with AI.

Clinical evaluation of glass-ionomer tunnel restorations in primary molars: 36 months results.

BACKGROUND: The purpose of this study was to clinically examine the efficacy of tunnel preparations in primary molars restored with glass-ionomer cement during a 36-month observation period. METHODS: A total of 233 tunnel restorations were placed in primary molars of 203 patients aged 6-10 years. Restorations were evaluated after 12, 24 and 36 months using the modified USPHS criteria for secondary caries, marginal discolouration, marginal adaptation and anatomic form. The Alpha+Bravo score (except for caries) and absence of clinical signs of endodontic complications was considered as a clinical success. RESULTS: At the end of the 36-month experimental period, the cumulative survival rate of tunnel restorations in primary molars was 72 per cent. The main reasons for failure were endodontic complications (46 per cent of the failures), secondary caries (31 per cent of the failures) and marginal ridge fractures (19 per cent of the failures). CONCLUSIONS: The tunnel preparation filled with reinforced restorative glass-ionomer cement is a suitable treatment for minimal proximal caries lesions in primary molars.

In vitro effectiveness of a chemo-mechanical method for caries removal.

AIM: The aim of the study was to assess the efficacy of chemo-mechanical method for caries removal based on histological analysis and scanning electron microscopy of dentine samples. METHODS: Forty-five freshly extracted human deciduous and permanent teeth with dentinal caries were used in this in vitro study. The teeth were allocated to two different technique groups. Chemo- mechanical caries removal using Carisolv was performed in 30 teeth and in 15 teeth caries was removed using conventional rotary instruments. The caries status of dentine was judged according to clinical criteria (probing and visual inspection). After caries removal had been completed, teeth were sectioned through the prepared cavities and the two halves of each tooth were processed for light microscopy and scanning electron microscopy. Light microscope was used to examine the specimens for the presence of bacteria in dentine tubules. Scanning electron microscope was used to determine surface characteristics of the cavity floor after caries removal. RESULTS: All examined dentine surfaces were clinically caries free. Regarding the presence of bacteria, 53% of chemo- mechanically prepared teeth and 87% of conventionally prepared teeth were bacteria-free (p>0.05). Efficacy of both methods was confirmed using scanning electron microscope, but different dentine topography was recorded after chemo-mechanical and conventional caries removal. CONCLUSION: Based on histological and scanning electron microscope analyses it could be concluded that this chemo-mechanical method is efficient in the removal of carious dentine.

Physiological profiles of male and female taekwon-do (ITF) black belts.

Baseline physiological and kinanthropometric data were collected for 11 male and 12 female elite taekwon-do athletes from the Czech national team for evaluation of anthropometry, aerobic and anaerobic capacities, strength, visual reaction time, pulmonary function, flexibility and explosive power of the lower limbs (vertical jump). Both male and female taekwon-do black belts demonstrated low adiposity (8.2 and 15.4% fat, BMI 21.9 and 22.0 kg m(-2), respectively), normal reactivity and pulmonary function, above average muscular strength, PWC-170 (3.4 vs 2.7 W kg(-1)) and aerobic power (54 vs 42 ml min(-1) kg(-1)), and a high flexibility (37 and 38 cm) and anaerobic performance (peak power output from a 30 s Wingate test=14.7 and 10.1 W kg(-1); anaerobic capacity=334 and 242 J kg(-1), in males and females, respectively). In male athletes, competitive performance was significantly related to maximum power output and upper limb reaction time only, whereas in females, performance was related to maximum power output and ventilatory threshold level. These variables accounted for 66 and 67% of the performance rank in males and females, respectively. Time-motion analysis of competition taekwon-do fighting (two times 2 min) revealed 3-5 s bouts of maximum exercise alternating with low-intensity periods. This elicits high heart rates (100% HRmax) and lactate responses (11.4 mmol l(-1) = 81% LAmax), which agrees well with the physiological characteristics of taekwon-do black belts measured in laboratory exercise tests.

The incidence and outcome of penetrating and blunt trauma in central Bosnia: the Nova Bila Hospital for War Wounded.

OBJECTIVES: Since February 1992, the civil war in the former Yugoslavia has left over 140,000 people dead. This study describes the injuries and outcome of patients treated at a provisional war hospital in Bosnia and compares mortality rates with war hospitals from prior armed conflicts. DESIGN: This is a retrospective review of 1,703 trauma patients treated between March 1993 and October 1993 at the Nova Bila War Hospital. RESULTS: In Bosnia, the overall mortality was 10.3% (5.0% dead on arrival and 5.3% inhospital deaths). Head injuries, seen in 19.2% of patients, had a mortality of 23.8%. The odds of sustaining a gunshot wound were 2.8 times greater in Bosnia than in Vietnam (p < 0.05). The odds of sustaining a head injury were 1.1 to 1.6 times greater in Bosnia than in Lebanon and Afghanistan (p < 0.05). The overall mortality odds in Bosnia were 3.1 times greater than in Vietnam (p < 0.001), but were equal to those in Lebanon and Afghanistan. CONCLUSIONS: Despite limited personnel and supplies, the inhospital mortality rate was comparable with those found in other war hospitals. The Nova Bila Hospital represents a unique response to the great medical need brought about by the continued fighting in the former Yugoslavia.