Reducing the negative impact of accidents associated with the release of dangerous substances to environment.

Background: The article is concerned with an evaluation of the current state of emergency readiness of industrial companies in the event of dangerous substance leakage and with a presentation of textile sorbents used for the purposes of capturing an escaped substance. Methods: A part of the article is concerned with the experimental designation of sorption capacity of hydrophobic, chemical, and universal sorption mats for chosen polar (water and alcohol) and non-polar (oil and gasoline) liquids. Experiments were realized according to Standard Test Method for Sorbent Performance of Adsorbents for use on Crude Oil and Related Spills, American Society for Testing and Materials (ASTM F726-17), type I. and Test methods for non-woven fabrics, European Union International Organization for Standardization (EN ISO 9073-6:2004). The aim of the article is an experimental designation of sorption capacity of textile sorption mats using two different methods, a comparison of the acquired results and a comparison of the acquired data with the data given by the manufacturer. Results: Textile sorbents, which can, owing to their sorption ability, allow the elimination or mitigation of a negative impact of a possible accident in the company connected with an escape of a liquid dangerous substance were tested and compared with the established values. Based on the obtained results it is possible to state that sorption capacities of the chemical and universal mat for the substrate water are equal and consistent with the data given by the manufacturer. Textile sorption mats also have a comparable sorption capacity. The sorption capacity on the substrate gasoline is the same in all textile sorbents. The adsorption capacity per unit mass all type's sorbents was similar for non-polar liquids (gasoline was values from 6.41 to 6.57 and oil was values from 9.54 to 10.24). Conclusion: The acquired results confirmed the universality of textile sorption mats for gasoline. Sorption capacities of the chemical and universal mat for the substrate water are equal and match the data given by the manufacturer. Textile sorption mats have a maximum sorption output up to 60 s, afterwards the sorption capacity values remain unchanged.

Citizens' Preparedness to Deal with Emergencies as an Important Component of Civil Protection.

The main purpose of this paper is to point out a new approach in evaluating the preparedness of the population of a selected city for civil protection and its response to emergencies. Using new approaches, it evaluates a subjective questionnaire survey in combination with the objective state thanks to a mathematical approach and its subsequent verification on a specific example. The proposed approaches are then verified by experimental surveys in the selected city. The result is a highly adaptable tool that can be set up and adapted to different situations and different types of questionnaires to address the preparedness and safety of the population for emergencies. Thanks to this tool, it is possible to evaluate the subjective opinions of the population and thus gain insight into the assessment of the city's preparedness for emergencies. Subsequently, we can set the prevention and preparedness of the population in the city on the basis of the obtained outputs, which potentially has a fundamental influence on the response after the occurrence of an emergency. Improving preparedness in the area of civil protection shall not only be reflected in the response and minimization of the consequences of the emergency, but also in the emotional security of the population.

Clostridium difficile ribotype 176 - A predictor for high mortality and risk of nosocomial spread?

PURPOSE: The objective of this survey was to determine the incidence of Clostridium difficile infections (CDI) at the Department of Infectious Diseases, Bulovka Hospital, and to evaluate clinical and epidemiological data on CDI patients together with a detailed molecular characterisation of C. difficile isolates. The patient outcomes were correlated to causative C. difficile PCR-ribotype. METHODS: The twelve-month study (2013) comprised patients two years of age and older with CDI. CDI severity was estimated using ESCMID criteria and ATLAS scoring. C. difficile isolates were further characterized using ribotyping, Multiple-Locus Variable Tandem-Repeats analysis (MLVA) and investigation of antibiotic-resistance determinants (gyrA, gyrB, rpoB, ermB). RESULTS: A total of 619 diarrhoeal stools were investigated. Seventy-two stool samples were GDH and toxin A/B positive, and 39 samples were GDH positive only and subsequently toxigenic C. difficile was cultured. In total, 111 C. difficile isolates were characterized, of which 64 (57.7%) belonged to PCR-ribotype 176. MLVA analysis of PCR-ribotype 176 isolates revealed 11 clonal complexes. Seventy-two isolates (64.9%) showed amino acid substitution Thr82Ile in the GyrA, and sixty-two isolates (55.9%) showed amino acid substitutions Arg505Lys together with His502Asn, or Asp492Glu together with Arg505Lys in the RpoB. Twelve isolates (10.8%) were ermB positive. Severe CDI according to the ESCMID criteria was recorded in forty-two patients (37.8%), and sixteen patients (14.4%) had ATLAS score ≥ 6. Twenty-nine patients (26.1%) had recurrent CDI and twenty-four patients (21.6%) died during the study period. CONCLUSIONS: A higher rate of severe CDI, recurrences and mortality in association with PCR-ribotype 176 infections were observed. The high incidence of PCR-ribotype 176 in the study, and the presence of clonal relatedness between PCR-ribotype 176 isolates, indicate its higher capacity to spread in a hospital setting, which in turn highlights the need to implement strict epidemic measures when PCR-ribotype 176 occurs.