RESUMEN
Differences in toxicity and carcinogenicity of the nephrotoxic compound aristolochic acid between rodents and humans suggest a species-dependent mechanism of action. The goal of this study was to investigate constitutive differences in the susceptibility of renal cortex cells originating from human, rat and porcine origin in vitro. Effects of 24 and 48 h AA exposure on cell number and MTT reduction were studied. Furthermore, using the effective concentrations causing 20 and 50% reduction (cell number), cell cycle, 3H-thymidine incorporation and DNA damage analyses were conducted. AA cytotoxicity was observed in all cell types in a time- and concentration dependent manner with species-specific differences, with porcine cells being the most sensitive. AA had a comparable effect on the cell cycle in primary human and porcine cells and the rat NRK-52E cell line following 48 h exposure, also corroborated by the reduced 3H-thymidine incorporation in NRK-52E cells. In addition, DNA unwinding, suggestive of enhanced DNA damage, was observed in primary porcine cells. These results provide an initial insight into the sensitivity and suitability of different in vitro-systems and suggest that primary porcine renal cortex cells could be a valuable in vitro-system to study AA toxicity.
Asunto(s)
Ácidos Aristolóquicos/toxicidad , Carcinógenos/toxicidad , Túbulos Renales Proximales/efectos de los fármacos , Porcinos , Animales , Ciclo Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , ADN/efectos de los fármacos , ADN/metabolismo , Relación Dosis-Respuesta a Droga , Células Epiteliales/efectos de los fármacos , Células Epiteliales/metabolismo , Células Epiteliales/patología , Formazáns/metabolismo , Humanos , Túbulos Renales Proximales/metabolismo , Túbulos Renales Proximales/patología , Valor Predictivo de las Pruebas , Ratas , Especificidad de la Especie , Sales de Tetrazolio/metabolismo , Timidina/metabolismo , TritioRESUMEN
Transrectal ultrasound-guided prostate biopsy is the standard technique to detect prostate cancer histologically. Based on a few case reports, it is known that prostate biopsy can cause a seeding of tumor cells. We report the case of a 64-year-old man who 2 years after radical prostatectomy for prostate cancer underwent proctocolectomy because of ulcerative pancolitis. In the lamina submucosa of the distal rectum a micrometastasis of the prostate cancer was found. A possible reason for this metastasis is an implantation of prostate cancer cells due to the transrectal biopsy of the prostate.
Asunto(s)
Adenocarcinoma/secundario , Biopsia con Aguja/efectos adversos , Colitis Ulcerosa/patología , Mucosa Intestinal/patología , Siembra Neoplásica , Complicaciones Posoperatorias/patología , Prostatectomía , Neoplasias de la Próstata/patología , Neoplasias del Recto/secundario , Vesículas Seminales/cirugía , Adenocarcinoma/patología , Transformación Celular Neoplásica/patología , Humanos , Hallazgos Incidentales , Masculino , Persona de Mediana Edad , Proctocolectomía Restauradora , Próstata/patología , Neoplasias del Recto/patología , Recto/patología , Vesículas Seminales/patologíaRESUMEN
In general, renal artery aneurysms occur very rarely. This study reports on a 9-cm renal artery aneurysm which was erroneously thought to be a renal cyst, and which, as a result of depression of the renal parenchym and the ureteropelvic junction, led to arterial hypertension and consecutive, reduced function hydronephrosis.
Asunto(s)
Aneurisma/complicaciones , Hidronefrosis/etiología , Hipertensión Renovascular/etiología , Arteria Renal , Aneurisma/diagnóstico por imagen , Angiografía de Substracción Digital , Errores Diagnósticos , Femenino , Humanos , Hidronefrosis/diagnóstico por imagen , Enfermedades Renales Quísticas/diagnóstico por imagen , Persona de Mediana Edad , Arteria Renal/diagnóstico por imagen , UltrasonografíaAsunto(s)
Carcinoma de Células Escamosas/cirugía , Neoplasias Renales/cirugía , Pelvis Renal , Adulto , Anciano , Carcinoma de Células Escamosas/patología , Humanos , Neoplasias Renales/patología , Pelvis Renal/patología , Pelvis Renal/cirugía , Masculino , Estadificación de Neoplasias , NefrectomíaAsunto(s)
Carcinoma in Situ/patología , Lesiones Precancerosas/patología , Neoplasias de la Vejiga Urinaria/patología , Biopsia , Cistitis/patología , Citofotometría , ADN de Neoplasias/metabolismo , Epitelio/patología , Humanos , Hiperplasia , Estadificación de Neoplasias , Vejiga Urinaria/patologíaRESUMEN
Report on a new, simple equipment for reproducible transrectal ultrasonographic examination of seminal vesicles and prostate. By means of this equipment it is possible to determine the exact extension of interesting areas, e.g. the size of the prostate and seminal vesicles.