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1.
Cell Tissue Bank ; 24(1): 265-272, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36042071

RESUMEN

The demand for skin tissue allografts to treat burns and other types of injuries increases each year to the extent that categories of donors formerly deemed "unsuitable", such as victims of suicide by polytrauma or poisoning, are now considered. Patients who died by ingestion of/exposure to toxic substances can be accepted as tissue donors after assessment of graft safety to rule out any risks of transferring toxic substances to the recipient. A cadaveric skin donation was obtained from a 57-year-old woman who died from intoxication after ingesting colchicine tablets (0.2 mg/kg). To determine the safety of cadaveric skin allografts, high-performance liquid chromatography-mass spectrometry (LC-MS/MS) was used to identify and quantify colchicine in procured skin. Results revealed that colchicine concentrations were lower than the instrument limit of detection (LOD) of 0.5 ng/mg both in epidermis and dermis. Cell viability assessed through the MTT ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]) test was within standard limits. Thanks to accurate tests performed, which are routinely applied also in clinical diagnostics and forensic toxicology, it was possible to ascertain the safety and suitability of skin tissue for donation.


Asunto(s)
Suicidio , Espectrometría de Masas en Tándem , Femenino , Humanos , Persona de Mediana Edad , Cromatografía Liquida , Colchicina , Muerte , Cadáver
2.
Orphanet J Rare Dis ; 15(1): 228, 2020 08 31.
Artículo en Inglés | MEDLINE | ID: mdl-32867855

RESUMEN

During the COVID-19 outbreak, the European Reference Network on Rare Bone Diseases (ERN BOND) coordination team and Italian rare bone diseases healthcare professionals created the "COVID-19 Helpline for Rare Bone Diseases" in an attempt to provide high-quality information and expertise on rare bone diseases remotely to patients and healthcare professionals. The present position statement describes the key characteristics of the Helpline initiative, along with the main aspects and topics that recurrently emerged as central for rare bone diseases patients and professionals. The main topics highlighted are general recommendations, pulmonary complications, drug treatment, trauma, pregnancy, children and elderly people, and patient associations role. The successful experience of the "COVID-19 Helpline for Rare Bone Diseases" launched in Italy could serve as a primer of gold-standard remote care for rare bone diseases for the other European countries and globally. Furthermore, similar COVID-19 helplines could be considered and applied for other rare diseases in order to implement remote patients' care.


Asunto(s)
Betacoronavirus , Enfermedades Óseas/complicaciones , Infecciones por Coronavirus/complicaciones , Neumonía Viral/complicaciones , Enfermedades Raras/complicaciones , Consulta Remota/normas , Anciano , Algoritmos , Enfermedades Óseas/terapia , COVID-19 , Niño , Infecciones por Coronavirus/prevención & control , Infecciones por Coronavirus/terapia , Femenino , Humanos , Pandemias/prevención & control , Neumonía Viral/prevención & control , Neumonía Viral/terapia , Embarazo , Enfermedades Raras/terapia , SARS-CoV-2 , Heridas y Lesiones
6.
Cell Tissue Bank ; 17(2): 241-53, 2016 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-26939692

RESUMEN

Skin allografts from cadaver donors are an important resource for treating extensive burns, slow-healing wounds and chronic ulcers. A high level of cell viability of cryopreserved allografts is often required, especially in burn surgery, in Italy. Thus, we aimed to determine which conditions enable procurement of highly viable skin in our Regional Skin Bank of Siena. For this purpose, we assessed cell viability of cryopreserved skin allografts procured between 2011 and 2013 from 127 consecutive skin donors, before and after freezing (at day 15, 180, and 365). For each skin donor, we collected data concerning clinical history (age, sex, smoking, phototype, dyslipidemia, diabetes, cause of death), donation process (multi-tissue or multi-organ) and timing of skin procurement (assessment of intervals such as death-harvesting, harvesting-banking, death-banking). All these variables were analysed in the whole case study (127 donors) and in different groups (e.g. multi-organ donors, non refrigerated multi-tissue donors, refrigerated multi-tissue donors) for correlations with cell viability. Our results indicated that cryopreserved skin allografts with higher cell viability were obtained from female, non smoker, heartbeating donors died of cerebral haemorrhage, and were harvested within 2 h of aortic clamping and banked within 12 h of harvesting (13-14 h from clamping). Age, cause of death and dyslipidaemia or diabetes did not appear to influence cell viability. To maintain acceptable cell viability, our skin bank needs to reduce the time interval between harvesting and banking, especially for refrigerated donors.


Asunto(s)
Criopreservación/métodos , Piel/citología , Bancos de Tejidos , Donantes de Tejidos , Supervivencia Tisular , Adolescente , Adulto , Anciano , Aloinjertos , Causas de Muerte , Supervivencia Celular , Femenino , Humanos , Italia , Masculino , Persona de Mediana Edad , Trasplante de Piel , Factores de Tiempo , Recolección de Tejidos y Órganos , Adulto Joven
7.
Transplant Proc ; 42(6): 2244-6, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-20692454

RESUMEN

Recently several types of skin equivalents have been developed, consisting of differentiated keratinocytes cultured on various dermal substitutes. Different models of reconstructed human skin have been proposed, such as human and animal de-epidermized dermis, inert filters, collagen matrices, lyophilized collagen membranes populated with fibroblasts, and other models populated with melanocytes and/or Langerhans cells. These skin equivalents mimic native skin in vivo. They have provided information about dermal-epidermal interactions, cell-cell, and cell-matrix interactions; responses of dermal and epithelial cells to biological signals and pharmacological agents; as well as effects of drugs and growth factors on wound healing. Human allodermis from tissue banks has been used for clinical purposes, namely, as support for autologous keratinocyte cultures and as a potentially ideal scaffold for dermal replacement. This bioproduct is considered to be the most suitable clinical carrier for autologous fibroblasts and keratinocytes, as well as an useful experimental model to study angiogenesis and to stimulate vascularization in reconstructed human skin. Because it is human-derived, it is in our opinion the safest of all available types of skin equivalent. Having epidermal and dermal structures, it can be used in one-stage grafting procedures for wound closure.


Asunto(s)
Dermis/citología , Piel/citología , Células Madre/citología , Adipocitos/citología , Antígenos CD34/análisis , Diferenciación Celular , Colágeno/análisis , Medios de Cultivo , Dermis/fisiología , Células Epidérmicas , Epidermis/fisiología , Sangre Fetal/citología , Fibroblastos/citología , Humanos , Células de Langerhans/citología , Melanocitos/citología , Mesodermo/citología , Piel Artificial , Células Madre/fisiología , Donantes de Tejidos
8.
Burns ; 36(3): 347-51, 2010 May.
Artículo en Inglés | MEDLINE | ID: mdl-19616385

RESUMEN

The Siena Skin Bank, established in 2000, processes skin from more than 130 cadaveric donors per year (about 400,000 cm(2)) and distributes it for transplants to treat burns and other types of skin loss. More than 1,500,000 cm(2) of homologous skin has been transplanted to date. At the Siena Skin Bank we conducted a retrospective study of our data to assess microbial contamination of skin specimens from 723 donors banked in the period 2000-2007. Our aim was to determine factors deleterious for skin quality, to optimize skin banking procedures and to reduce corrective actions. The factors analyzed were: type of donor (multi-organ, multi-tissue, live or cadaver), cause of death, time elapsing between death and procurement, different procurement centres and operator experience. Of the 723 donors considered, 26.55% (192/723) were positive for microbes, 22.68% (164) for bacteria and 5.39% (39) for mycetes. Of these 192 positives, 82 (42.70%) required corrective actions. The data obtained showed that the only variables significantly affecting microbial contamination of tissue were type of donor (live or cadaver) and type of processing (cryo- or glycerol preservation).


Asunto(s)
Trasplante de Piel/normas , Piel/microbiología , Bancos de Tejidos/normas , Adolescente , Adulto , Anciano , Arthrodermataceae/aislamiento & purificación , Bacterias/aislamiento & purificación , Femenino , Humanos , Masculino , Persona de Mediana Edad , Donantes de Tejidos , Conservación de Tejido/métodos , Recolección de Tejidos y Órganos/métodos , Adulto Joven
9.
J Dermatolog Treat ; 15(3): 179-81, 2004 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-15204151

RESUMEN

BACKGROUND: Ingrowing toenails are a common problem causing significant morbidity. Many of the procedures used for treating this painful condition cause considerable discomfort and have high failure rates. In recent years, phenol cauterization of the nail bed has been used increasingly. METHODS: A retrospective study was carried out of all patients treated by this method in the authors' clinic during the period January 1996 to December 2001. In this study, phenol cauterization is the standard form of treatment and 948 phenol cauterizations were carried out in 764 patients. RESULTS: There was no significant morbidity and the procedure was tolerated well by all patients. Overall recurrence was 4.3% (33 patients) after 18 months. All symptomatic recurrences were successfully treated by repeating phenol cauterization. CONCLUSIONS: Phenol cauterization compares favourably with other surgical ablation techniques for ingrowing toenails and may be the ablation procedure of choice of an ingrowing toenail.


Asunto(s)
Cauterización/métodos , Uñas Encarnadas/epidemiología , Uñas Encarnadas/cirugía , Fenol/administración & dosificación , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Italia/epidemiología , Masculino , Registros Médicos , Persona de Mediana Edad , Uñas Encarnadas/patología , Recurrencia , Estudios Retrospectivos , Resultado del Tratamiento
10.
J Wound Care ; 13(5): 202-4, 2004 May.
Artículo en Inglés | MEDLINE | ID: mdl-15160576

RESUMEN

OBJECTIVE: Hyaluronan, a component of the extracellular matrix, plays a significant role in several aspects of tissue repair and the wound healing process. METHOD: In this Italian study Hyalofill-F, a partial benzyl ester derivative of hyaluronan, used in combination with compression bandaging, was compared with the well-established therapy in Italy of non-adherent gauze plus compression therapy in the treatment of chronic venous leg ulcers. RESULTS: Hyalofill-F plus compression bandaging performed significantly better than non-adherent gauze plus compression bandage in all of the clinically relevant efficacy parameters. Mean reduction in ulcer area in the hyaluronan-derivative group was 8.1 cm2 after eight weeks of treatment, compared with 0.4 cm2 in the comparator group. The resulting difference of 7.7 cm2 between the two groups was statistically significant (p = 0.0019). Furthermore, statistically significant results in favour of the hyaluronan-derivative group were obtained in the following: speed of epithelialisation; leveling of the margins; degree of maceration; pain intensity and frequency. CONCLUSION: Hyalofill-F plus compression bandaging resulted in an earlier and greater decrease in ulcer area compared with non-adherent gauze plus compression bandaging, therapy supporting its use in the treatment of chronic venous ulcers.


Asunto(s)
Adyuvantes Inmunológicos/uso terapéutico , Ácido Hialurónico/análogos & derivados , Ácido Hialurónico/uso terapéutico , Úlcera Varicosa/tratamiento farmacológico , Vendajes , Humanos , Modelos Lineales
11.
Artículo en Inglés | MEDLINE | ID: mdl-16335623

RESUMEN

Biocompatibility of metals for dental use was tested using a three-dimensional model consisting of oral keratinocytes cultured on de-epidermised sub-mucosa. The toxicity of orthodontic metallic wire and soldering material was assessed through parameters such as the morphology and growth rate of the keratinocytes, as well as by classical histology, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The sharp composition of metallic wires and soldering materials was assessed by Auger Electron Spectroscopy (AES). The results of our experiment showed that the new model revealed inhibition of keratinocyte growth and stratification near soldering material, whereas mucosal cells were able to grow and layer out on dental wire. It is concluded that this experimental model, which simulates the oral environment, is useful for studying the effects of materials for dental use for its sensibility and reproducibility. Moreover it can provide morpho-functional information which cannot be achieved by traditional methods.


Asunto(s)
Aleaciones Dentales/toxicidad , Soldadura Dental/efectos adversos , Queratinocitos/efectos de los fármacos , Alambres para Ortodoncia/efectos adversos , Técnicas de Cultivo de Célula , Células Cultivadas , Fibroblastos/efectos de los fármacos , Humanos , Ensayo de Materiales , Filtros Microporos , Microscopía Electrónica , Mucosa Bucal , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Análisis Espectral/métodos
12.
J Eur Acad Dermatol Venereol ; 17(6): 680-3, 2003 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-14761136

RESUMEN

BACKGROUND: Cutaneous photoageing is a complex biological process affecting all layers of the skin. Skin damage resulting from intrinsic ageing and extrinsic photoageing may trigger skin cancer. In patients with advanced photoageing and/or diffuse actinic damage, local therapy is often inadequate and the possibility of combined therapy needs to be assessed. SUBJECTS: Here we report three cases of patients over 75 years of age with advanced diffuse epithelial skin damage of photoexposed areas consisting of several superficial actinic keratoses, ipermelanotic lesions and multiple skin cancers. METHODS: Neoplastic lesions and damaged skin were removed by superficial erbium laser ablation and the epidermis reconstructed with autologous epidermal sheets expanded in vitro from healthy cells obtained from unexposed areas of the body. RESULTS: Our initial studies show that this procedure is very effective in the short term for treating and preventing the UV-induced skin cancer and precancerous lesions, and also suggest good long-term control of the disease with very interesting aesthetic results.


Asunto(s)
Terapia por Láser/métodos , Envejecimiento de la Piel/patología , Trasplante de Piel/métodos , Luz Solar/efectos adversos , Rayos Ultravioleta/efectos adversos , Factores de Edad , Anciano , Anciano de 80 o más Años , Terapia Combinada , Epitelio/patología , Epitelio/efectos de la radiación , Femenino , Estudios de Seguimiento , Humanos , Masculino , Medición de Riesgo , Muestreo , Índice de Severidad de la Enfermedad , Neoplasias Cutáneas/patología , Neoplasias Cutáneas/prevención & control , Neoplasias Cutáneas/cirugía
13.
Pediatr Dermatol ; 18(5): 422-5, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11737690

RESUMEN

Rothmund-Thomson syndrome (RTS) is a genetic disease characterized by developmental abnormalities and poikilodermatous skin changes that appear in infancy. An association with myelodysplastic syndromes is rarely reported in RTS, even though impairment of immune function and recurrent infections are described in the literature. A case of Thomson-type RTS in a 14-year-old girl with trilinear myelodysplasia is presented. The patient was kept under hematologic surveillance for myelodysplastic syndrome. Bone marrow transplantation was considered unnecessary at present.


Asunto(s)
Síndromes Mielodisplásicos/complicaciones , Síndrome Rothmund-Thomson/complicaciones , Adolescente , Femenino , Humanos
14.
Hum Mutat ; 18(1): 85, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11439001

RESUMEN

Pseudoxanthoma elasticum (PXE) is a mendelian disorder characterized by calcification of elastic fibers in skin, arteries, and retina. It results in dermal lesions, arterial insufficiency and retinal hemorrhages, leading to macular degeneration. PXE is transmitted either as an autosomal dominant or recessive trait and several sporadic cases have been observed. Mutations in the ABCC6 gene have been identified very recently in patients. Here, we report on a large Italian family affected by pseudoxanthoma elasticum for which linkage analysis had pointed to a region encompassing markers D16S3069-D16S405-D16S3103; hemizygosity of marker D16S405 allowed us to detect a submicroscopic deletion of at least 900 kb involving ABCC6, ABCC1, and MYH11. Mutation analysis on the other allele of the family, as well as on two additional sporadic cases, revealed nonsense (Y227X, R518X, R1164X) and frame-shift (c.960delC) mutations in ABCC6 (MRP6) further confirming the role of this multi-drug resistance gene in the etiology of pseudoxanthoma elasticum. Furthermore, clinical re-examination of members of the family harboring the deletion led to the detection of additional features, potentially caused by the deletion of the MYH11 gene. In the course of the analysis five nonpathogenic variants were found in ABCC6: 1233T>C, 1245G>A, 1838 T>G (V614A), 1890C>G, and 3506+83C>A. Hum Mutat 18:85, 2001.


Asunto(s)
Proteínas Asociadas a Resistencia a Múltiples Medicamentos/genética , Cadenas Pesadas de Miosina/genética , Mutación Puntual/genética , Seudoxantoma Elástico/genética , Eliminación de Secuencia/genética , Miosinas del Músculo Liso/genética , Adulto , Anciano , Alelos , Mapeo Cromosómico , Análisis Mutacional de ADN , Exones/genética , Femenino , Marcadores Genéticos/genética , Haplotipos/genética , Humanos , Italia , Masculino , Linaje , Seudoxantoma Elástico/patología
15.
J Am Acad Dermatol ; 44(5): 862-4, 2001 May.
Artículo en Inglés | MEDLINE | ID: mdl-11312438

RESUMEN

We report the case of a patient in whom Sweet's syndrome developed during pneumonia caused by Chlamydia pneumoniae. Increased expression of helper T-cell type 1 cytokine secretion pattern in peripheral blood has recently been observed in patients with this syndrome, and chlamydia infection is known to primarily activate a helper T-cell type 1 immunologic response.


Asunto(s)
Infecciones por Chlamydia/diagnóstico , Neumonía Bacteriana/diagnóstico , Síndrome de Sweet/diagnóstico , Infecciones por Chlamydia/complicaciones , Infecciones por Chlamydia/diagnóstico por imagen , Chlamydophila pneumoniae/aislamiento & purificación , Diagnóstico Diferencial , Femenino , Humanos , Persona de Mediana Edad , Neumonía Bacteriana/complicaciones , Neumonía Bacteriana/diagnóstico por imagen , Radiografía , Síndrome de Sweet/complicaciones , Síndrome de Sweet/patología
16.
Clin Exp Dermatol ; 26(1): 53-5, 2001 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-11260181

RESUMEN

Multiple minute digitate hyperkeratosis (MMDH) is a skin disease of unknown aetiology characterized clinically by multiple minute asymptomatic keratotic lesions with spiky horny projections. The disorder has been classified into early (congenital) and late (acquired) onset forms, the latter occurring as a presenting sign of concomitant inflammatory, metabolic or malignant disease. Here we report two cases of late onset MMDH without any associated pathology. These cases emphasize that some cases of late-onset MMDH may be idiopathic.


Asunto(s)
Queratosis/patología , Anciano , Anciano de 80 o más Años , Emolientes/uso terapéutico , Femenino , Humanos , Queratolíticos/uso terapéutico , Queratosis/tratamiento farmacológico , Persona de Mediana Edad , Resultado del Tratamiento , Negativa del Paciente al Tratamiento
17.
J Dermatolog Treat ; 12(1): 25-8, 2001 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-12171684

RESUMEN

We report the case of a road accident victim who sustained fracture of the frontal bone and extensive skin loss. Immediate repair of the skin breach was necessary to protect the set bone fragments. Intraoperative skin expansion provided an immediate gain in tissue and avoided the waiting period required with traditional expansion methods. Besides an excellent immediate functional result, acceptable aesthetic results, confirmed 6 months later, were also obtained.


Asunto(s)
Cuero Cabelludo/cirugía , Fracturas Craneales/cirugía , Expansión de Tejido/métodos , Accidentes de Tránsito , Humanos , Cuidados Intraoperatorios , Cuero Cabelludo/lesiones , Fracturas Craneales/etiología , Colgajos Quirúrgicos , Dispositivos de Expansión Tisular
20.
Biomaterials ; 20(18): 1689-94, 1999 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-10503969

RESUMEN

Here, a three-dimensional model based on fragments of human de-epidermized dermis (DED) is prepared in order to study the performance of a microperforated, hyaluronan-based membrane as a carrier of cultured epidermal cells. Hyaluronic acid is, in fact, considered to be an optimal biomaterial allowing proliferation of both keratinocytes and melanocytes, and it is already used for clinical aims. The carrier with subconfluent human epidermal cultures is positioned onto the DED and kept in culture until a new epidermis is formed. This model system allowed to study the migration and growth of human epidermal cells from the carrier, resembling 'in vivo' re-epithelization.


Asunto(s)
Adyuvantes Inmunológicos/farmacología , Ingeniería Biomédica/métodos , Epidermis/crecimiento & desarrollo , Ácido Hialurónico/farmacología , Membranas Artificiales , Materiales Biocompatibles/farmacología , Diferenciación Celular , Células Cultivadas , Humanos , Modelos Biológicos
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