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1.
Zhonghua Fu Chan Ke Za Zhi ; 56(6): 425-433, 2021 Jun 25.
Artículo en Chino | MEDLINE | ID: mdl-34154318

RESUMEN

Objective: To investigate the differential expression of long non-coding RNA (lncRNA) in placental tissues of women with preeclampsia (PE) and the effect of MIR210HG on the biological function of HTR8/SVneo cells. Methods: A total of 39 cases of PE women (PE group) and 39 cases of normal pregnant women (CTL group) admitted to the Affiliated Hospital of Qingdao University from July 2018 to July 2019 were collected. (1) Transcriptome sequencing (RNA-seq) was used to analyze the differentially expressed lncRNAs in the placental tissues of the two groups. (2) The expression level of MIR210HG, one of the differentially expressed lncRNAs, in the placental tissues of the two groups was detected by real-time quantitative PCR. And the correlations between the expression level of MIR210HG and systolic blood pressure, diastolic blood pressure and neonatal birth weight were analyzed. (3) The constructed small interfering RNA and negative control (NC) RNA were transfected into the HTR8/SVneo cells. The cells were divided into MIR210HG knockdown (KD) group and NC group. The effects of living cell counting (CCK-8) and transwell assay on the proliferation and migration of HTR8/SVneo cells were detected. (4) RNA interacting with MIR210HG was predicted using the Encyclopedia of RNA Interactomes (ENCORI) database. Gene Ontology (GO) functional annotation, Kyoto Encyclopedia of Gene and Genomes (KEGG) and BioCarta pathway enrichment analysis were performed. Results: (1) A total of 26 significantly differentially expressed lncRNAs were found by RNA-seq, among which 21 lncRNAs were up-regulated and 5 lncRNAs were down-regulated. (2) The relative expression level of MIR210HG in the PE group was significantly higher than that in the CTL group (9.30±1.90 and 1.10±0.20, respectively; t=4.425, P<0.01). The relative expression level of MIR210HG had positive linear correlation with systolic blood pressure (r2=0.234, P<0.05) and diastolic blood pressure (r2=0.190, P<0.05), but had a negative linear correlation with newborn birth weight (r2=0.157, P<0.05). (3) Compared with the NC group, the proliferation and migration ability of HTR8/SVneo cells in the KD group were increased (all P<0.05). (4) A total of 38 RNAs that might interact with MIR210HG were predicted by ENCORI database. GO functional annotation analysis showed that MIR210HG might be involved in the functions of 27 pathways, including the regulation of production of molecular mediator of immune response, etc; KEGG pathway analysis showed that MIR210HG might be involved in the function of 8 pathways including allograft rejection, etc; Biocarta pathway analysis showed that MIR210HG may be involved in the functions of 8 pathways, including the eukaryotic initiation factor (eIF) pathway, etc. Conclusion: The expression of MIR210HG is up-regulated in the placental tissue of PE women, and MIR210HG might be a regulator of the biological behavior of trophoblast cells.


Asunto(s)
Placenta , Preeclampsia , ARN Largo no Codificante , Femenino , Humanos , Recién Nacido , Preeclampsia/genética , Embarazo , ARN Largo no Codificante/genética , ARN Interferente Pequeño , Trofoblastos
2.
Zhonghua Jie He He Hu Xi Za Zhi ; 15(4): 214-6, 254, 1992 Aug.
Artículo en Chino | MEDLINE | ID: mdl-1339159

RESUMEN

The levels of free radicals in the whole blood of the patients with cor pulmonale were determined using the method of electron spin resonance (ESR). The concentrations of serum SOD were determined using RIA. The concentrations of the MDA, vitamin E, selenium and the activities of the SeGSHPx, CAT of the patients were also measured using biochemical methods. The results showed that the level of free radical in the whole blood and serum MDA, SOD increase if compared with that of control group. The activities of SeGSH-Px, CAT and the concentration of vitamin E in serum of the patients are in lower level. These indicate that there is impairment in free radical metabolism of the patients.


Asunto(s)
Enfermedad Cardiopulmonar/metabolismo , Anciano , Catalasa/sangre , Espectroscopía de Resonancia por Spin del Electrón , Femenino , Radicales Libres , Glutatión Peroxidasa/sangre , Humanos , Peroxidación de Lípido , Masculino , Malondialdehído/sangre , Persona de Mediana Edad , Enfermedad Cardiopulmonar/enzimología , Superóxido Dismutasa/sangre
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