Cadmium accumulation and metallothionein synthesis in freshwater bivalves (Pyganodon grandis): relative influence of the metal exposure gradient versus limnological variability.

The relative influence of limnological confounding factors on cadmium (Cd) bioaccumulation and metallothionein (MT) synthesis was quantified in natural populations of freshwater bivalves (Pyganodon grandis) living in lakes along a Cd concentration gradient. During the ice-free period, we measured 15 environmental variables in the water compartment and determined total concentrations of Cd and MT in the gills of bivalves at 37 littoral stations in 20 lakes distributed across the mining area of Rouyn-Noranda in northwestern Quebec. A multiple linear regression model including pH (+), dissolved Ca concentrations (-) and free Cd2+ concentrations at the sediment-water interface (+) explained 74% of the variability in Cd concentrations in the bivalve gills. Dissolved Ca (-) and free Cd2+ (+) together explained 62% of the variation in MT concentrations in the bivalve gills. Partial linear regression analyses indicated that the limnological factors' pure and shared effects together accounted for 48 and 45% of the total variation in Cd and MT concentrations in the gills, respectively. A lake selection procedure that could be applied in monitoring programs is proposed to minimise the relative influence of these confounding variables.

Periphyton as an indicator of water quality in the St Lawrence River (Québec, Canada).

The performance of various algal indices to document improvements in water quality across a low nutrient concentration gradient was assessed during 2 years in the St Lawrence River (Quebec, Canada). Water-quality variables and periphyton samples were collected on navigational buoys near Montreal during the spring, summer and fall of 1994 and 1995. Exposure to urban wastewater varied widely within the sector surrounding the island of Montreal, with some areas upstream receiving no direct effluents and areas further downstream receiving treated and untreated wastewater. Faecal coliform concentrations provided a good tracer of effluents and were significantly correlated to nutrient concentrations (r = 0.33-0.72, p < 0.001) and water transparency (r = 0.70, p < 0.001). Despite a strong gradient in faecal coliform concentration (< 2 to > 20 000 UFC/100 ml), algal biomass and diversity did not reflect differences between sites with varying levels of urban wastewater. Taxonomic composition of periphyton communities, particularly the presence of the cyanophyte Plectonema notatum Schmidle, was related (r = 0.48, p = 0.004) to exposure to urban effluents. Variables describing seasonal changes (temperature, Julian day, river discharge, conductivity, NO2-NO3) explained a large fraction of total variance (38-52% of total variance) and thus exerted the predominant influence on algal biomass and species composition in the St Lawrence River. Variables describing the presence of effluents explained 1-22% of the variance in compositional data. Subtle changes in periphyton species composition were the only response to different levels of exposure to urban wastewater in the Montreal area, which represented relatively small differences in comparison to natural seasonal variability.

Macroinvertebrate community as a biological indicator of ecological and toxicological factors in Lake Saint-François (Québec).

To assess the potential of the macroinvertebrate community for monitoring variation in the environmental quality of large rivers, the response of littoral macrobenthos in Lake Saint-François, a fluvial lake of the St Lawrence River (Québec) are described. First, the composition of total macroinvertebrate communities and important taxonomic groups as well as the biotic ICI-SL index in 16 littoral stations varying in sedimentology, water chemistry and contamination are described to define indicator species groups and environmental quality ranks. Thereafter, the relative contribution of ecological and toxicological factors in explaining the variation observed in macroinvertebrate assemblages and biotic index were quantified using partial canonical correspondence analysis. Cluster analyses based on taxonomic composition separated five groups of stations where macroinvertebrate assemblages varied in density, composition and tolerance to pollution. The ICI-SL biotic index varied from 7.2 to 27.2 with a mean value of 19 +/- 6. The ICI-SL values determined for the macroinvertebrate communities in Lake Saint-François did not reflect an important deterioration in environmental quality, and there was some agreement between the environmental quality ranking of the stations expressed either by the ICI-SL index or the community cluster analysis. Water conductivity and phosphorus concentration, followed by macrophyte types (Chara, Ceratophyllum) and sediment grain size, were the most significant ecological variables to explain variation in macroinvertebrate communities and derived ICI-SL index in Lake Saint-François. Among the toxicological factors, metals in water (Fe, Cr, Pb, Mn, Zn) and sediment (Mn, Pb, Se), as well as the composite indices of metal and organic contamination (water CI, sediment CI, sediment total PAHs) were the most important factors. The contamination factors selected in our models represented contaminant sorption processes rather than direct toxicological effects. The lack of clear relationships between contaminants and macroinvertebrate variables reflected the relative low level of contamination in the stations sampled in Lake Saint-François. There were some interactions between toxicological and ecological variables that should be considered in the planning of sampling and interpretation of biomonitoring studies. However, the large amount of unexplained variance (49.2-86.6%) in the CCA models underlined the limitations of the use of the indices of macroinvertebrate community structure that were assessed in this study for biomonitoring purposes in the absence of a contrasting pollution gradient.

Epiphyte size and taxonomy as biological indicators of ecological and toxicological factors in Lake Saint-François (Québec).

To assess the potential of the epiphyton community for monitoring spatial and temporal variations in the environmental quality of freshwater ecosystems, this paper describes the response of epiphytons to ecological and toxicological factors in Lake Saint-François, a fluvial lake of the St. Lawrence River (Québec). First, community descriptions and biotic indices based on size structure and on taxonomic composition were compared to test if size could usefully describe epiphyton response to environmental factors. Secondly, the relative contribution of ecological and toxicological variables in explaining the variation observed in epiphyton was quantified. A size-based approach classified the sites similarly to taxonomic analysis, but a larger portion of variance in size could be explained indicating a stronger relationship with environmental factors. Among both taxonomy and size-based biotic indices, the slope of the normalized size spectra performed best in terms of total explained variance. There were strong interactions between toxicological and ecological variables. These interactions should be considered in the planning and interpretation of biomonitoring studies. In such surveys, samples should be collected at sites with similar ecological characteristics: for example, the same macrophyte type and sediment texture. Interpretation of the data should consider the possible covariance of toxicological and ecological variables.

Short-term variations in specific biovolumes of different bacterial forms in aquatic ecosystems.

Short-term and spatial fluctuations in specific biovolumes (volume x cell(-1)) of different morphological categories of planktonic bacteria were estimated microscopically. Samples were taken from two lakes occurring in two different climatic systems: Lake Aydat (France) and Lake Cromwell (Canada). The study was done in summer, using 24-hour cycles of sampling.Due to their large size, the specific volume of filamentous bacteria constituted, on average, the major part (>70%) of the total specific volume of all bacterial forms considered. Greatest variations in specific biovolumes were recorded for filamentous bacteria (coefficients of variation ranged from 16 to 109%). These variations were more pronounced in the oxygenated and microaerophilic strata (DOC ≈1.5 mg liter(-1)). Fluctuations in cell volume were high (coefficients of variation =12-80%) for coccal bacteria, whereas no marked fluctuations were found for the rod and vibrio bacteria (coefficients of variation =4-10%).Evidence of diel patterns of cell volume of filamentous bacteria is provided. These cells displayed their maximum size during the day until early night, indicating cell division was occurring at night. Homogeneous circadian patterns were not provided by specific volume variations of coccal, rod, and vibrio bacteria.Statistical relationships between bacterial specific biovolumes and the biotic and abiotic parameters considered are discussed.