RESUMEN
OBJECTIVES: We investigated effects of chronic exposure (2 months) to ambient levels of particulate matter (PM) on development of protease-induced emphysema and pulmonary remodeling in mice. METHODS: Balb/c mice received nasal drop of either papain or normal saline and were kept in two exposure chambers situated in an area with high traffic density. One of them received ambient air and the other had filters for PM. RESULTS: mean concentration of PM10 was 2.68 +/- 0.38 and 33.86 +/- 2.09 microg/m3, respectively, in the filtered and ambient air chambers (p < 0.001). After 2 months of exposure, lungs from papain-treated mice kept in the chamber with ambient air presented greater values of mean linear intercept, an increase in density of collagen fibers in alveolar septa and in expression of 8-isoprostane (p = 0.002, p < 0.05 and p = 0.002, respectively, compared to papain-treated mice kept in the chamber with filtered air). We did not observe significant differences between these two groups in density of macrophages and in amount of cells expressing matrix metalloproteinase-12. There were no significant differences in saline-treated mice kept in the two chambers. CONCLUSIONS: We conclude that exposure to urban levels of PM worsens protease-induced emphysema and increases pulmonary remodeling. We suggest that an increase in oxidative stress induced by PM exposure influences this response. These pulmonary effects of PM were observed only in mice with emphysema.
Asunto(s)
Contaminantes Atmosféricos/toxicidad , Enfisema/patología , Emisiones de Vehículos , Animales , Inmunohistoquímica , Exposición por Inhalación , Macrófagos/efectos de los fármacos , Metaloproteinasa 12 de la Matriz/metabolismo , Ratones , Alveolos Pulmonares/efectos de los fármacos , Alveolos Pulmonares/enzimología , Alveolos Pulmonares/metabolismoRESUMEN
Cutaneous manifestations in disseminated strongyloidiasis are infrequent but should raise the suspicion for its diagnosis. We retrospectively evaluated the charts of six patients with cancer and a proven diagnosis of disseminated strongyloidiasis. All patients had received prophylaxis with albendazole before starting antineoplastic therapy, which included high-dose steroids. They presented with septic shock, acute respiratory failure and characteristic purpuric periumbilical skin lesions. Strongyloides larvae were identified in tracheal aspirates (n=5), gastric aspirates (n=4), lung (n=2) and skin biopsies (n=2). All patients died despite antihelminthic therapy and intensive care support.
Asunto(s)
Adulto , Anciano , Animales , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neoplasias/parasitología , Neoplasias/patología , Púrpura/patología , Enfermedades Cutáneas Parasitarias/patología , Strongyloides stercoralis/aislamiento & purificación , Estrongiloidiasis/patología , Antihelmínticos/uso terapéutico , Biopsia , Resultado Fatal , Huésped Inmunocomprometido , Neoplasias/inmunología , Púrpura/inmunología , Púrpura/parasitología , Enfermedades Cutáneas Parasitarias/complicaciones , Piel/parasitología , Piel/patología , Estrongiloidiasis/complicaciones , Estrongiloidiasis/tratamiento farmacológicoRESUMEN
Cutaneous manifestations in disseminated strongyloidiasis are infrequent but should raise the suspicion for its diagnosis. We retrospectively evaluated the charts of six patients with cancer and a proven diagnosis of disseminated strongyloidiasis. All patients had received prophylaxis with albendazole before starting antineoplastic therapy, which included high-dose steroids. They presented with septic shock, acute respiratory failure and characteristic purpuric periumbilical skin lesions. Strongyloides larvae were identified in tracheal aspirates (n=5), gastric aspirates (n=4), lung (n=2) and skin biopsies (n=2). All patients died despite antihelminthic therapy and intensive care support.