Induction of a tomato peroxidase gene in vascular tissue.

Expression of a tomato peroxidase gene that is constitutively expressed only in roots was induced in stems and leaves as a result of mechanical wounding. However, wound-induction of TPX1 transcript accumulation in leaves was limited to the mid-rib. No TPX1 transcript was detected in the lamina of the leaf after wounding. Peroxidase isozyme studies indicated the presence of a unique basic isoform in stems after wounding.

Characterization and in situ localization of a salt-induced tomato peroxidase mRNA.

NaCl treatment of tomato plants in hydroponic culture at concentrations as low as 50 mM resulted in enhanced accumulation of transcripts of TPX1, a full-length cDNA clone that we had isolated from a library of NaCl-treated tomato plants using a peroxidase-specific oligonucleotide probe. Although the overall amino acid sequence identity of TPX1 to other peroxidase genes was less than 45%, there was a very high degree of identity in all of the conserved domains. The deduced amino acid sequence included the presence of a N-terminal signal peptide but not the C-terminal extension present in peroxidases targeted to the vacuole. The mature protein has a theoretical pI value of 7.5. Transcripts that hybridized to TPX1 were detected only in the roots with higher levels of mRNA in epidermal and subepidermal cell layers. Isoelectric focusing of root extracts showed two major bands of peroxidase activity at pI 5.9 and 6.2. Both activities increased with salt treatment. Southern analysis indicated the presence of only a single TPX1 gene in tomato.

[The city of Puebla in the regional context. I:ndustrialization and marginality]. / La ciudad de Puebla en el contexto regional. Industrializacion y marginalidad.

PIP: The city of Puebla concentrates 25.6% of the total population of the state of Puebla, 30% of its employed population, 42% of its secondary sector workers, and 52% of its tertiary sector workers. Puebla has historically been an intermediate point between Mexico City and the states of Veracruz, Chiapas, Tabasco, and Oaxaca. Its geographic location and importance as a pioneer in the country's textile industry and later its industrial diversification have allowed Puebla to be functionally complementary to Mexico City. Puebla's regional influence and territorial integration of neighboring areas are increasing. State governments of Puebla over the past 30 years have promoted these tendencies as a means of fortifying Puebla's economic and political position. Puebla's municipal boundaries have been expanding since 1960 to accommodate industrial parks and an industrial corridor along the Mexico City-Puebla highway. The boundaries have moved outward to incorporate urbanized neighboring settlements. Intraregional development has benefited the municipio of Puebla more than its neighbors. Puebla is the only municipio in the region considered to exhibit a very low degree of marginality. In the past 20 years, the 14 municipios in the region have nearly doubled their population, from 728,974 to 1,445,447 inhabitants. The municipio of Puebla has maintained a relative weight of about 73% of the regional total. Current trends suggest that Puebla's relative demographic importance will decline within the region. The ongoing diversification of economic activities should tend to reduce intraregional disparities.^ieng

Angiodisplasia pancreatico-duodenal asociada a carcinoide del pancreas. / Pancreatic-duodenal angiodysplasia associated with carcinoid of the pancreas

Una mujer de 41 anos de edad se presento al hospital con sangrado gastro-intestinal cronico y anemia severa. Por arteriografia se demostro una malformacion arterio-venosa (angiodisplasia) de la segunda porcion del duodeno y la cabeza del pancreas con llenado prematuro de la vena porta. En la intervencion quirurgica realizada se encontraron vasos anormales originados en la arteria pancreaticoduodenal y ademas en la cabeza del pancreas se encontro un tumor de 3 x 3 cm. Se realizo pancreatoduodenectomia. Histologicamente se confirmo la presencia de un carcinoide maligno del pancreas con metastasis regionales y multiples vasos anormales