Can toothbrushing reduce the intraoral viral load of SARS-CoV-2? A pilot study with a dentifrice containing an antimicrobial phthalocyanine derivative.

The aim of this study was to assess whether toothbrushing with a dentifrice containing an antimicrobial phthalocyanine derivative (APD) can reduce the intraoral viral load of SARS-CoV-2. Twenty COVID-19-positive dentate patients aged ≥18 years were selected instructed to brush their teeth for 2 min with a dentifrice containing APD. Self-collected samples of unstimulated saliva were carried out three times: T0 (baseline), T5 (5 min after toothbrushing), and T30 (30 min after toothbrushing). The analysis of viral RNA was performed by RT-qPCR for detection of three viral genes (ORF1ab, N and S genes). Results were statistically tested using Friedman's test and pairwise comparison with Bonferroni corrections, with a significance level of 5%. There was an increase in the cycle threshold (Ct) value from T0 to T5 in 13 patients (72.2%), and from T0 to T30 in 14 patients (77.8%). In two patients (11.1%) no SARS-CoV-2 was detected at T5 and five patients (27.8%) at T30. The Ct values were statistically significantly higher (p=0.020) at T30 in comparison to T0 and T5. This pilot study suggests that toothbrushing with a dentifrice containing APD could reduce the SARS-CoV-2 viral load in the oral cavity. However, further studies are needed to confirm this possible beneficial effect against SARS-CoV-2.

USE OF MOUTHWASH AND DENTIFRICE CONTAINING AN ANTIMICROBIAL PHTHALOCYANINE DERIVATIVE FOR THE REDUCTION OF CLINICAL SYMPTOMS OF COVID-19: A RANDOMIZED TRIPLE-BLIND CLINICAL TRIAL.

PURPOSE: This clinical trial aimed to evaluate the use of mouthwash and dentifrice containing an antimicrobial phthalocyanine derivative (APD) to reduce the clinical symptoms in patients with COVID-19. METHODS: This randomized, triple-blind clinical trial enrolled 134 patients aged 18 years or older who underwent COVID-19 testing through the use of nasopharyngeal swab RT-qPCR in a reference center for the diagnosis of COVID-19, had no clinical contraindications to mouthwash and gargle, and had access to cell phones with communication applications. According to the use of a mouthwash and dentifrice containing antimicrobial phthalocyanine derivatives (APD), patients were randomly assigned (1:1) to the APD or non-APD (control) group. All participants were instructed to floss twice a day, brush teeth for 2 minutes 3 times a day, and gargle/rinse (5 mL) for 1 min/3 times a day for 7 days. An online questionnaire was sent to collect data on the clinical symptoms of COVID-19 3 times: T0 (baseline before using the oral hygiene products), T3 (3 days after), and T7 (7 days after). The investigators, patients, and outcome assessors were blinded to group assignment. The Mann-Whitney, Chi-Square, Fisher's exact, and Cochran's tests were used according to the nature of the variables studied, with the level of significance set at P < .05. RESULTS: No statistically significant difference was found in the prevalence of symptoms between groups at baseline. A statistically significant reduction in clinical symptoms was found in the control group (fatigue, shortness of breath, hoarse voice, sore throat, nasal congestion, and chest pain) and APD group (cough, fatigue, shortness of breath, hyposmia/anosmia, dysgeusia, hoarse voice, sore throat, nasal congestion, chest pain, diarrhea, and irritability/confusion) during the follow-up period. There were statistically significant differences, with a higher prevalence of symptoms in the control group at T3 and T7. Dysgeusia, sore throat, and irritability/confusion were less prevalent in the APD group at T3, and shortness of breath, hyposmia/anosmia, dysgeusia, hoarse voice, sore throat, diarrhea, and irritability/confusion were more prevalent in the control group at T7. CONCLUSIONS: Based on this methodology, the results demonstrated that the regular use of mouthwash and dentifrice-containing APD had a positive impact on the clinical symptoms, as reported by patients with COVID-19.

A single session of antimicrobial photodynamic therapy does not influence the alveolar repair process in rats.

The aim of this study was to use microscopic and molecular techniques to evaluate the effects of a single session of antimicrobial photodynamic therapy (aPDT) on the alveolar repair process after tooth extraction in rats. The study sample included 84 rats divided into four groups, as follows: a) Control - untreated socket; b) Laser - socket treated using photobiomodulation; c) TBO - socket treated with topic application of the photosensitizer agent, toluidine blue O (TBO); and d) aPDT - socket treated with TBO and laser irradiation. An additional rat was used for thermal mapping during socket irradiation. The animals were euthanatized at 6, 15, and 28 days after unilateral extraction of the upper incisor. Quantitative and qualitative analyses of the connective and bone tissues, blood clot, blood vessel, and inflammatory infiltrate were performed, and real-time polymerase chain reaction was used to study the expression of genes (collagen type I, osteocalcin, alkaline phosphatase [ALP], runt-related transcription factor 2 [RUNX2], and vascular endothelial growth factor [VEGF]) involved in the bone healing process. No statistically significant differences in microscopic and molecular outcomes were observed between the groups (p > 0.05). A positive correlation was seen to exist between blood clot and VEGF (p = 0.000), and a negative correlation was observed between bone tissue and ALP (p = 0.028) and blood vessel and VEGF (p = 0.018). A single session of aPDT in the dental extraction site did not influence the alveolar repair process in rats.

A single session of antimicrobial photodynamic therapy does not influence the alveolar repair process in rats

Abstract: The aim of this study was to use microscopic and molecular techniques to evaluate the effects of a single session of antimicrobial photodynamic therapy (aPDT) on the alveolar repair process after tooth extraction in rats. The study sample included 84 rats divided into four groups, as follows: a) Control - untreated socket; b) Laser - socket treated using photobiomodulation; c) TBO - socket treated with topic application of the photosensitizer agent, toluidine blue O (TBO); and d) aPDT - socket treated with TBO and laser irradiation. An additional rat was used for thermal mapping during socket irradiation. The animals were euthanatized at 6, 15, and 28 days after unilateral extraction of the upper incisor. Quantitative and qualitative analyses of the connective and bone tissues, blood clot, blood vessel, and inflammatory infiltrate were performed, and real-time polymerase chain reaction was used to study the expression of genes (collagen type I, osteocalcin, alkaline phosphatase [ALP], runt-related transcription factor 2 [RUNX2], and vascular endothelial growth factor [VEGF]) involved in the bone healing process. No statistically significant differences in microscopic and molecular outcomes were observed between the groups (p > 0.05). A positive correlation was seen to exist between blood clot and VEGF (p = 0.000), and a negative correlation was observed between bone tissue and ALP (p = 0.028) and blood vessel and VEGF (p = 0.018). A single session of aPDT in the dental extraction site did not influence the alveolar repair process in rats.

Reliability and accuracy of automatic segmentation of mandibular 3D models on linear measurements.

OBJECTIVE: Evaluate if automatic segmentation of mandibular three-dimensional (3D) models is reliable and accurate. MATERIALS AND METHODS: Eight dry mandibles with eight silica markers were scanned in the i-CAT Classic device (Imaging Sciences International). Automatic segmentation was performed using nine standard preset thresholds in the Dolphin software (Dolphin Imaging & Management Solutions). Three observers individually made twice eight linear measurements on the mandibular 3D models. Another observer made physical measurements, twice as well, on the dry mandibles. Reliability and accuracy were evaluated with intraclass correlation coefficients (ICCs), Dahlberg's formula, Bland-Altman analyses, and changing bias with regression analyses. RESULTS: Inter-observer and intra-observer ICCs and Dahlberg's error were ≥ 0.75 and ≤ 1.0 mm, respectively, for all measurements. Inter-observer agreement between mandibular 3D models and physical measurements ranged from -0.37 to 0.91 mm. CONCLUSIONS: Linear measurements made on mandibular 3D models obtained using standard preset thresholds are reliable and accurate. However, additional studies are necessary to confirm this hypothesis for clinical applications. CLINICAL RELEVANCE: Since the 3D models are useful for diagnostics and surgical planning, it is necessary to determinate whether the linear measurements made on 3D models obtained by automatic segmentation are sufficiently reliable and accurate.

Analysis of the Preventative Influence of an Oral Hygiene Protocol on Ventilator-Associated Pneumonia / Análise da Influência Preventiva de um Protocolo de Higiene oral na Pneumonia Associada à Ventilação Mecânica

Ventilator-associated pneumonia (VAP) is the most commonly reported nosocomial infection among intensive care unit (ICU) patients. This study aimed to evaluate the influence of an oral hygiene protocol on VAP incidence among mechanically-ventilated (MV) ICU patients. The dentate patients admitted without VAP to the hospital's adult ICU requiring mechanical ventilation for ≥48 hours during a 4-month period in two consecutive years (2015 ­ Control group and 2016 ­ Experimental group) were included in this investigation. The oral hygiene protocol was implemented daily, once a day, in the morning hours, using a disposable brush with suction and ultrasoft bristles and 3 g of 0.12% chlorhexidine gel. Hospital-related respiratory infection data, including VAP diagnoses and microbiological analyses, were collected from the monthly health epidemiological notification bulletins issued by the hospital. T tests were applied to compare variables before versus after implementation of the oral hygiene protocol with a significance criterion of P < .05. 43.94% reduction in VAP rate after the protocol implementation was observed. Implementation of the protocol was associated with a significant reduction of Enterobacter spp infections and no cases of VAP related to the main etiological agents of the disease (i.e., S. aureus and C. albicans) were identified. It can be concluded that an oral hygiene protocol performed with a suction brush and 0.12% chlorhexidine gel can serve as an effective prophylaxis against VAP in patients under mechanical ventilation. (AU)

A pneumonia associada à ventilação (PAV) é a infecção nosocomial mais comumente relatada em pacientes de Unidade de Terapia Intensiva (UTI). Este estudo teve como objetivo avaliar a influência de um protocolo de higiene bucal na incidência de PAV entre pacientes sob ventilação mecânica (VM). Os pacientes dentados internados na UTI adulta do hospital sem PAV e que necessitaram de ventilação mecânica por ≥ 48 horas durante um período de 4 meses em dois anos consecutivos (2015 - grupo Controle e 2016 - grupo Experimental) foram incluídos nesta investigação. O protocolo de higiene bucal foi implementado diariamente, uma vez ao dia, nas primeiras horas da manhã, utilizando-se escova descartável com cerdas de sucção e ultramacia e 3 g de gel de clorexidina a 0,12%. Dados de infecção respiratória relacionada ao hospital, incluindo diagnósticos de PAV e análises microbiológicas, foram coletados dos boletins mensais de notificação epidemiológica de saúde emitidos pelo hospital. Testes t foram aplicados para comparar as variáveis antes e após a implementação do protocolo de higiene bucal com um critério de significância de P <0,05. Observamos uma redução de 43,94% na taxa de PAV após a implementação do protocolo A implementação do protocolo foi associada à uma redução significativa das infecções por Enterobacter spp e não foram identificados casos de PAV relacionados aos principais agentes etiológicos da doença (isto é, S. aureus e C. albicans). Pode-se concluir que u protocolo de higiene bucal realizado com escova de sucção e gel de clorexidina a 0,12% pode servir como uma profilaxia efetiva contra a PAV em pacientes sob ventilação mecânica. (AU)

Analysis of the Gray-Value Reproducibility and Noise of a Direct Digital Radiography System / Análise da Reprodutibilidade do Valor de Cinza e Ruído de um Sistema de Radiografia Digital Direto

The digital radiograph represents a great advance in oral maxillofacial radiology because it incorporates informatics technology in the capture, interpretation, and archiving of radiographic images. Previous studies have demonstrated that it is possible to use gray values in bone lesion diagnosis and follow-up. However, these applications depend on radiograph system quality and exposure time. The aim of this study was to evaluate the gray-value reproducibility and noise produced by Dabi Atlante's IDA system, a direct digital radiography system. Radiographs were obtained in a standardized manner (70 kV, 7 mA, and 2.2-mm filtration) with a direct digital sensor and a stepwedge placed in a phantom at a 30-cm focus-film distance. Ten consecutive x-ray imaging series were completed at 0.10-s, 0.15-s, and 0.20-s exposure times. Gray values were analyzed in five regions of interest (ROIs): bone tissue (BT), soft tissue (ST), and three stepwedge steps (Step 1, Step 2, and Step 3). Mean gray values differed significantly across exposure times (p < .05) in all five ROIs. The ROI with the greatest gray-value variability (25.36%) and noise (9.46%) was ST. In conclusion, gray-value reproducibility and noise of the IDA system vary across areas with differing radiolucency. Thus, special attention is necessary for the diagnosis and follow-up of radiolucent lesions due to relatively high gray-value interference (AU).

A radiografia digital representa um grande avanço na radiologia bucomaxilofacial porque incorpora a tecnologia informática na captura, interpretação e arquivamento de imagens radiográficas. Estudos anteriores demonstraram que é possível usar os valores de cinza no diagnóstico e na proservação das lesões ósseas. No entanto, essas aplicações dependem da qualidade do sistema radiológico e do tempo de exposição. O objetivo deste estudo foi avaliar a reprodutibilidade do valor de cinza e o ruído produzido pelo sistema IDA da Dabi Atlante, um sistema de radiografia digital direto. As radiografias foram obtidas de maneira padronizada (70 kV, 7 mA e filtração de 2,2 mm) com um sensor digital direto e um penetrômetro colocados em um fantoma a uma distância de filme-foco de 30 cm. Dez imagens radiográficas consecutivas foram obtidas com tempos de exposição de 0,10-s, 0,15-s e 0,20-s. Os valores de cinza foram analisados em cinco regiões de interesse (ROIs): tecido ósseo (TO), tecido mole (TM) e três degraus do penetrômetro (Degrau 1, Degrau 2 e Degrau 3). Os valores de cinza médios diferiram significativamente entre os tempos de exposição (p <0,05) em todos as cinco ROIs. A ROI com maior variabilidade do valor de cinza (25,36%) e ruído (9,46%) foi TM. Em conclusão, a reprodutibilidade do valor de cinza e o ruído do sistema IDA variam entre áreas com radiolucência diferente. Assim, atenção especial é necessária para o diagnóstico e proservação de lesões radiolucentes devido à interferência dos valores cinza relativamente alta. (AU).

Panorama situacional dos cursos de Habilitação em Odontologia Hospitalar: revisão integrativa / Situational overview of the courses of Qualification in Hospital Dentistry: integrative review

Cirurgiões-dentistas habilitados em práticas baseadas em evidências científicas e na aplicação de modelos de cuidados em saúde bucal bem-sucedidos trazem benefícios tanto à saúde individual quanto à coletiva. Este estudo traçou um panorama sobre cursos de Habilitação em Odontologia Hospitalar ofertados no Brasil após a regulamentação da Resolução 162/2015 pelo Conselho Federal de Odontologia (CFO), para auxiliar a escolha dos profissionais que pretendem se habilitar na área. O método de pesquisa foi a revisão integrativa da literatura, com análise quantitativa, exploratória e descritiva dos dados. O modelo de busca aplicado foi "habilitação em odontologia hospitalar", na base de dados Google, com uma única consulta em março de 2019. Dezessete (17) propostas foram encontradas. Seguiram para análise treze (13) propostas que informavam número de vagas ofertadas, periodicidade do curso, dias da semana em que ocorriam as atividades, carga horária total, conteúdo programático (teórico e prático) e local da prática. Falta de conteúdo programático (n=2) e de atualização (n=1) foram critérios de exclusão imediata. Na análise descritiva das propostas (n=10) houve exclusões por falta de definição da carga horária teórica e prática (n=4); informação sobre o conteúdo prático (n=5) e de identificação do local de desenvolvimento e carga horária mínima exigida para a prática. O estudo evidenciou que, à época da coleta de dados, apenas uma proposta atendia à legislação e à normativa exigidas pelo CFO (AU).

Dentists qualified on evidence-based practices and on the application of successful oral health care models are beneficial to individual and collective health. The current study presents an overview of Hospital Dentistry Qualification courses available in Brazil after the regulation of Resolution 162/2015 by the Conselho Federal de Odontologia (CFO - Brazil), which focuses on assisting the selection by professionals who intend to qualify in this field. Integrative literature review, with quantitative, exploratory and descriptive data analyses, was the research method adopted in the present study. The mesh "hospital dentistry qualification" was used in the single search conducted on Google database, in March 2019. Seventeen (17) proposals were found. Thirteen (13) proposals that included information about vacancies available for students, course periodicity, weekdays when the course took place, total workload, program content (theoretical and practical) and place of practice were selected for analysis. Lack of program content (n=2) and outdated information (n=1) were immediate exclusion criteria. The descriptive analysis applied to the proposals (n=10) has shown that courses were excluded due to lack of definition ofthe workload of theoretical and practical activities (n=4); as well as of information about the practical content of the course (n=5), the hospital where the practice would take place and the minimum workload required for the practice. One (01) qualification proposal was included in the research based on qualitative synthesis. The current study has shown that one proposal was in compliance with the legislation and regulations required by FCD, as well as that the presentation of the information available in the website about the proposal makes it hard for the target audience to make the option for the course that would best meet their needs (AU).

Dimensional Stability of Alginates Molds Tridimentionally Scanned / Estabilidade Dimensional de Moldes de Alginatos Escaneados Tridimensionalmente

Alginate is among the most used materials in dentistry to create teeth negative printing and reproduction. The goal of this study was to compare dimensional changes of alginate scanned impression materials. Thirty impressions of a standard typodont were performed using three types of alginate (Hydrogum 5; Jeltrate Plus and Avagel). The impressions were scanned by the scanner 3Shape R700T and scans were performed immediately after molding. The analysis of digital models were performed at OrthoAnalyzer ™ 3D software. Measurements were carried out in relation to the transverse dimension (intercanine, interpremolares and intermolar). Method error was evaluated through the Intraclass Correlation Coefficient (ICC) and Bland-Altman. One-way ANOVA, Dunnet Post-test were used to compare the different groups in relation to Typodont (gold standard) for the different outcome variables. The data were tabulated in Statistical Package for Social Sciences version 20.0 and Minitab 17.0 softwares to compare groups. The significance level was 5%. The posterior transverse variables (D1PM, D2PM and DM) presented a statistically significant difference regarding the gold standard (Typodont) for the plaster models performed after the Avagel molding. In the digital models performed just after molding, only in DM, the Avagel material group presented values statistically higher than the control group. The alginates Hydrogum 5 and Jeltrate Plus presented dimensions closer to the gold standard.(AU).

O alginato está entre os materiais de moldagem mais utilizados na Odontologia para criar impressão e reprodução negativa dos dentes. O objetivo desse trabalho foi comparar alterações transversais de três marcas comerciais de alginato (Hydrogum, Jeltrate e Avagel) em moldes de alginatos. Trinta moldes de um Typodont padrão foram realizados, utilizando três tipos de alginato (Hydrogum 5; Jeltrate Plus e Avagel). Os moldes foram escaneados por meio do scanner 3Shape R700T e os escaneamentos realizados logo após a moldagem e logo após a obtenção dos modelos de gesso. As análises dos modelos digitais foram realizadas no software OrthoAnalyzer™ 3D. Foram realizadas mensurações em relação à discrepância transversal (inter-caninos, inter-primeiro pré-molar, inter-segundo pré-molar e inter-molares). O erro do método foi avaliado por meio do Coeficiente de Correlação Intraclasse (CCI) e Bland-Altman. Utilizou-se Análise de Variância (one-way ANOVA, Pós-teste Dunnet) para comparação dos diferentes grupos em relação ao typodont (padrão-ouro) para as diferentes variáveis de desfecho. A análise estatística foi realizada nos programas Statistical Package for Social Sciences versão 20.0 e no programa Minitab 17.0 para comparação dos grupos. O nível de significância foi de 5%. As variáveis transversais posteriores (D1PM; D2PM e DM) apresentaram diferença estatisticamente significante em relação ao padrão-ouro (manequim) para os modelos de gesso realizados após a moldagem com Avagel. Nos modelos digitais realizados logo após a moldagem, apenas na DM, o grupo do material Avagel apresentou valores estatisticamente maiores do que o grupo controle. Os alginatos Hydrogum 5 e Jeltrate Plus apresentaram dimensões mais próximas do padrão-ouro.(AU).