Cellular Responses of Gelidium floridanum (Gelidiales, Rhodophyta) Tetraspores Under Heat Wave and Copper Pollution.

Spore settlement and development are bottlenecks for resilience of habitat-forming macroalgal species. These processes are directly related to temperature, a global stressor protagonist of ocean warming. The toxic effects of local pollutants such as copper may be worsened under a global warming scenario. Therefore, in this paper, we investigated the effects of increased temperature combined with elevated concentrations of copper on the viability, photosynthetic pigments, and ultrastructure of Gelidium floridanum tetraspores. Tetraspores were cultivated on slides with sterilized seawater or seawater enriched with CuCl2 , and incubated under 24°C or 30°C for 24 h. Tetraspores cultivated with copper 3.0 µM under 30°C had lower viability. Both temperature and copper had a significant effect on phycocyanin and phycoerythrin concentrations. Samples cultivated with copper under 30°C presented a heavily altered cellular structure, with vesicles throughout the cytoplasm, chloroplasts with altered structure and cells with degenerated cytoplasm and cell walls. Our findings show that temperature and copper significantly affect the viability, photosynthetic pigments, and ultrastructure of G. floridanum tetraspores, presenting an additive interaction for the physiology of this seaweed's early stages.

Ocean warming and copper pollution: implications for metabolic compounds of the agarophyte Gelidium floridanum (Gelidiales, Rhodophyta).

Ocean warming is increasing and scientific predictions suggest a rise of up to 4°C in sea water temperatures. The combination of a polluted and warmer environment may be detrimental for aquatic species, especially for primary producers such as seaweeds. This study investigated the potential for interactive effects of an increased seawater temperature in a copper-rich environment on the photosynthetic pigments and metabolic compounds of the red seaweed Gelidium floridanum. Seaweed samples were cultivated in a factorial design with temperature (24°C and 30°C), copper (0 and 3 µM), and time (7 and 14 d). The exposure of G. floridanum to copper and 30°C for 7 d resulted in a lower concentration of chlorophyll a, smaller phycobiliprotein rods and lower concentration of soluble sugars. After 14 d of cultivation, a higher concentration of chlorophyll a and soluble sugars could be observed on seaweeds cultivated under 30°C. The accumulation of carotenoids and the release of phenolic compounds indicated specific protective mechanisms against temperature and copper, respectively. Overall, seaweeds grew less when exposed to copper 3 µM at 30°C.