RESUMEN
Taraxastane, oleanane, ursane, lupane, taraxane, cycloartane, dammarane and tirucallane triterpenoids isolated from flowers of Compositae plants have been previously reported to exhibit anti-inflammatory effects and are variously competitive and non-competitive inhibitors of the serine proteases trypsin and chymotrypsin. The general features of those triterpenoids found to be protease inhibitors are having a hydroxy group and an appropriate side chain in the region of the molecule distal to the 3-hydroxy group. However, fatty acid esterification of the triterpenoid 3-hydroxy group can have a marked effect on inhibitor effectiveness. This suggests a possible means of rapid alteration of the plant defensive complement in vivo and of the bioactivity of these anti-inflammatory compounds.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Asteraceae/química , Quimotripsina/efectos de los fármacos , Triterpenos/farmacología , Inhibidores de Tripsina/farmacología , Tripsina/efectos de los fármacos , Antiinflamatorios no Esteroideos/química , Extractos Vegetales/farmacología , Tallos de la Planta/química , Triterpenos/química , Inhibidores de Tripsina/químicaRESUMEN
The lupane triterpenoid lupeol, the ursane triterpenoid alpha-amyrin and esters of these compounds are present in the bark of roots of Alstonia boonei (Apocynaceae) and have anti-inflammatory properties. alpha-Amyrin is a competitive inhibitor of bovine trypsin and chymotrypsin (Ki values 29 microM and 18 microM, respectively). Lupeol linoleate, lupeol palmitate and alpha-amyrin linoleate are non-competitive inhibitors of trypsin (Ki values 7 microM, 10 microM and 16 microM, respectively). alpha-Amyrin linoleate is also a non-competitive inhibitor of chymotrypsin (Ki value 28 microM). Lupeol is a competitive inhibitor of both trypsin and chymotrypsin (Ki values 22 and 8 microM, respectively). alpha-Amyrin palmitate is a potent non-competitive inhibitor of chymotrypsin (Ki 6 microM). Lupeol, alpha-amyrin and the palmitic and linoleic acid esters of these compounds are ineffective or very weak as inhibitors of porcine pancreatic elastase and of Lucilia cuprina and Helicoverpa punctigera leucine aminopeptidases. These hydrophobic triterpenoids represent further examples of anti-inflammatory triterpenoids that are PKA inhibitors as well as being selective protease inhibitors.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Inhibidores de Serina Proteinasa/farmacología , Triterpenos/farmacología , Animales , Bovinos , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidoresRESUMEN
The ursane triterpenoid alpha-amyrin and the lupane triterpenoid lupeol are potent inhibitors of the catalytic subunit (cAK) of rat liver cyclic AMP-dependent protein kinase (PKA) with IC50 values of 8 and 5 microM, respectively. The palmitate and linoleate esters of alpha-amyrin and lupeol are also potent inhibitors of cAK (IC50 values in the range of 4-9 microM). alpha-Amyrin, lupeol and lupeol linoleate are much less potent as inhibitors of rat brain Ca(2+)- and phospholipid-dependent protein kinase (PKC) (IC50 values 32, 82 and 35 microM; respectively) and alpha-amyrin linoleate and the palmitate esters of lupeol and alpha-amyrin are ineffective or very poor inhibitors of this protein kinase. These compounds are very poor or ineffective as inhibitors of chicken gizzard calmodulin-dependent myosin light chain kinase (MLCK). alpha-Amyrin inhibits plant Ca(2+)-dependent protein kinase (CDPK) (IC50 52 microM) but lupeol and the triterpenoid esters tested are ineffective. alpha-Amyrin and the linoleate and palmitate esters of alpha-amyrin and lupeol inhibit cAK in a fashion that is competitive with respect to both peptide substrate and ATP (Ki values in the range 2-6 microM). However, while lupeol is competitive with respect to ATP it is apparently non-competitive with respect to peptide substrate. alpha-Amyrin also inhibits CDPK competitively and alpha-amyrin, lupeol and lupeol linoleate are competitive inhibitors of PKC. alpha-Amyrin and the palmitate esters of lupeol and alpha-amyrin are competitive inhibitors of the potato high affinity cyclic AMP-binding phosphatase (Pase) but lupeol inhibits the Pase non-competitively. These hydrophobic triterpenoids are further examples of anti-inflammatory triterpenoids that are cAK inhibitors.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Inhibidores Enzimáticos/farmacología , Inhibidores de Proteínas Quinasas , Triterpenos/farmacología , Secuencia de Aminoácidos , Animales , Antiinflamatorios no Esteroideos/química , Inhibidores Enzimáticos/química , Cinética , Datos de Secuencia Molecular , Ácido Oleanólico/análogos & derivados , Triterpenos Pentacíclicos , Ratas , Triterpenos/químicaRESUMEN
A series of prenylated xanthones are variously potent inhibitors of the catalytic subunit (cAK) of rat liver cyclic AMP-dependent protein kinase (PKA), rat brain Ca2+ and phospholipid-dependent protein kinase C (PKC), chicken gizzard myosin light chain kinase (MLCK), wheat embryo Ca2+-dependent protein kinase (CDPK) and potato tuber cyclic nucleotide-binding phosphatase (Pase). The prenylated xanthones examined are mostly derivatives of alpha-mangostin in which the 3-hydroxyl and 6-hydroxyl are variously substituted with groups R or R', respectively, or derivatives of 3-isomangostin (mangostanol) in which the 9-hydroxyl is substituted with groups R' or the prenyl side chain is modified. The most potent inhibitors of cAK have non-protonatable and relatively small R' and R groups. Conversely, the most potent inhibitors of PKC and MLCK have bulkier and basic R' groups. Some prenylated xanthones are also potent inhibitors of CDPK. PKC and cAK are competitively inhibited by particular prenylated xanthones whereas the compounds that are the most potent inhibitors of MLCK and CDPK are non-competitive inhibitors. Prenylated xanthones having relatively small and non-protonatable R' and R groups inhibit a high-affinity cyclic nucleotide binding Pase in a non-competitive fashion.
Asunto(s)
Antifúngicos/farmacología , Proteínas Quinasas Reguladas por Nucleótidos Cíclicos/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Inhibidores de Proteínas Quinasas , Proteínas Quinasas , Xantenos/farmacología , Xantonas , Animales , Antifúngicos/aislamiento & purificación , Pollos , Molleja de las Aves/enzimología , Quinasa de Cadena Ligera de Miosina/antagonistas & inhibidores , Ratas , Solanum tuberosum/enzimología , Relación Estructura-Actividad , Triticum/enzimología , Xantenos/químicaRESUMEN
The protein tyrosine kinase (PTK) inhibitor piceatannol is also an inhibitor of the rat liver cyclic AMP-dependent protein kinase (PKA) catalytic subunit (cAK), rat brain Ca(2+)- and phospholipid-dependent protein kinase C (PKC), avian gizzard Ca(2+)-calmodulin-dependent myosin light chain kinae (MLCK), and of wheat embryo Ca(2+)-dependent protein kinase (CDPK) (IC50 values 3, 8, 12, and 19 microM, respectively). However, a number of piceatannol-related compounds with fewer or no phenolic hydroxy substituents are inactive or very poor inhibitors of these serine/threonine protein kinases. Similarly, the PTK inhibitor ellagic acid is a potent inhibitor of cAK and of PKC (IC50 values 2 and 8 microM, respectively), whereas the non-phenolic perylene is ineffective as a protein kinase inhibitor. Ellagic acid is a competitive inhibitor of both cAK and of PKC but piceatannol inhibits these enzymes in a fashion that is competitive and non-competitive, respectively. Interaction with calmodulin may contribute to the inhibition of MLCK and CDPK by piceatannol.
Asunto(s)
Inhibidores Enzimáticos/farmacología , Proteínas Serina-Treonina Quinasas/antagonistas & inhibidores , Estilbenos/farmacología , Animales , Encéfalo/enzimología , Ácido Elágico/farmacología , RatasRESUMEN
The aporphine isoquinoline alkaloid apomorphine is a potent inhibitor of the catalytic subunit (cAK) of rat liver cyclic AMP-dependent protein kinase (PKA), myosin light chain kinase (MLCK), and Ca(2+)- and phospholipid-dependent protein kinase C (PKC) (IC50 values 1, 11, and 8 microM, respectively). However, a number of O-methylated analogues of apomorphine are inactive or poor inhibitors of cAK. The benzophenanthridine isoquinoline alkaloid sanguinarine is a potent inhibitor of cAK but is a relatively poor inhibitor of PKC (IC50 values 6 and 217 microM respectively). However a number of methylated analogues of sanguinarine are inactive as cAK inhibitors. The aporphine isoquinoline alkaloids (+)-boldine and bulbocapnine are non-competitive inhibitors of MLCK with respect to both peptide substrate and ATP. The inhibition of cAK, MLCK, and PKC by apomorphine and sanguinarine is competitive with respect to ATP as substrate. The oxazine alkaloids darrow red, nile blue A, and oxazine 170 are variously effective as inhibitors of cAK, MLCK, PKC, and CDPK (IC50 values 4-65 microM). Ca2+ binds to apomorphine and (+)-boldine which, together with nile blue A and oxazine 170, are potent inhibitors of calmodulin (CaM)-dependent MLCK (IC50 values 11, 12, 4, and 7 microM, respectively), and interact with dansyl-CaM.
Asunto(s)
Alcaloides/farmacología , Inhibidores Enzimáticos/farmacología , Inhibidores de Proteínas Quinasas , Secuencia de Aminoácidos , Animales , Pollos , Datos de Secuencia Molecular , RatasRESUMEN
The basic protein fraction from seeds of castor bean (Ricinus communis L.) contains 4732 Da and 4603 Da proteins phosphorylated in vitro by plant Ca(2+)-dependent protein kinase (CDPK). These proteins, RS1A and RS1B respectively, were purified by cation-exchange HPLC (SP5PW column) and reverse-phase HPLC (C18 column) and identified as napin-like protein small chains by Edman sequencing and electrospray ionization mass spectrometry (ESMS). The other R. communis 4 kDa small chains (RS2A, RS2B, RS2C and RS2D) are not phosphorylated by CDPK and neither is the corresponding 7332 Da large chain (RL) that forms 1:1 disulfide-linked complexes with RS2(A-D). RS1A/B is one of the best substrates found for plant CDPK (K(m) = 1.8 +/- 0.8 microM). RS2(A-D) (but not RL or RS1A/B) strongly inhibit calmodulin (CaM)-dependent myosin light chain protein kinase (MLCK) (IC50 = 0.25 microM) and inhibit the Ca(2+)-dependent enhancement of dansyl-CaM fluorescence. The basic protein fraction from seeds of bitter melon (Momordica charantia) also contains napin-like proteins that are 1:1 disulfide-linked complexes of a small chain (MS1, MS2, MS3 or MS4) and a large chain (ML). The M. charantia small chains were purified and completely sequenced by Edman degradation and ESMS. M. charantia small chains MS1, MS2, and MS4 (but not MS3) are phosphorylated by CDPK to unit stoichiometry on S21 within the sequence R17SCES21FLR. The R. communis small chain RS1A is phosphorylated on S34 within the sequence R31QSS34SRR. Both of these phosphorylation site motifs are consistent with those found for other plant CDPK substrates.
Asunto(s)
Proteínas de Plantas/aislamiento & purificación , Plantas Tóxicas , Proteínas Quinasas/metabolismo , Ricinus communis/química , Albuminas 2S de Plantas , Secuencia de Aminoácidos , Sitios de Unión , Calmodulina/antagonistas & inhibidores , Cromatografía Líquida de Alta Presión , Frutas/química , Datos de Secuencia Molecular , Fragmentos de Péptidos/química , Fosforilación , Proteínas de Plantas/química , Semillas/químicaRESUMEN
Condensed tannins, isolated from a variety of plant sources, were characterized according to the constituent flavans, being based on procyanidin and/or prodelphinidin and having a cis or trans stereochemistry at positions 2 and 3. All the tannin preparations are potent inhibitors of rat liver cyclic AMP-dependent protein kinase catalytic subunit (cAK) with IC50 values (concentrations for 50% inhibition) ranging from 0.009 to 0.2 microM. The tannin preparations are very good inhibitors of rat brain Ca(2+)- and phospholipid-dependent protein kinase C (PKC) (IC50 values in the range 0.3-7 microM), wheat embryo Ca(2+)-dependent protein kinase (CDPK) (IC50 values in the range 0.8-7 microM) and of calmodulin (CaM)-dependent myosin light chain kinase (MLCK) (IC50 values in the range 7-24 microM). One of the most effective preparations, that from the leaves of Ribes nigrum, has IC50 values with respect to cAK, PKC, CDPK and MLCK of 0.009, 0.6, 2.0 and 16 microM, respectively. In general, the order with respect to sensitivity to inhibition by these condensed tannins is cAK > PKC > CDPK > MLCK. The Ribes nigrum preparation is a competitive inhibitor of cAK with respect to both ATP and synthetic peptide substrate. These condensed tannin preparations are the most potent plant-derived inhibitors of cAK yet found.
Asunto(s)
Inhibidores Enzimáticos/farmacología , Plantas , Inhibidores de Proteínas Quinasas , Taninos/farmacología , Animales , Encéfalo/enzimología , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Cinética , Hígado/enzimología , Quinasa de Cadena Ligera de Miosina/antagonistas & inhibidores , Hojas de la Planta , Proteína Quinasa C/antagonistas & inhibidores , Ratas , Semillas , Estereoisomerismo , Taninos/química , Taninos/aislamiento & purificación , Triticum/enzimologíaRESUMEN
A large series of isoquinoline derivatives was synthesised including derivatives of isoquinoline, isoquinolino[3,4-c]furazan, 1,2-dihydro-1-oxoisoquinoline, 6-oxopyrimido[1,2-d]isoquinoline, benzo[c][1,8]-naphthyridine, pyrazino[2,3-c]isoquinoline and benzimidazo[2,1-a]isoquinoline as well as further structurally related isoquinoline derivatives and pyrido-2,3-furazans. Representatives of all of these classes of isoquinolines are potent and selective inhibitors of the cyclic AMP-dependent protein kinase (PKA) catalytic subunit (cAK) from rat liver. The most effective cAK inhibitors are a series of 1,3-di-substituted and 1,3,4-tri-substituted isoquinolines (IC50 values 30-50 nM) (compounds A1, A2, A3, A4 and A5) and 2-ethylcarboxy-3-amino-5,6-dihydro-6-oxobenzo[c] [1,8]naphthyridine (E1) (IC50 0.08 microM). Compounds A1-A5 inhibit cAK in a fashion that is competitive with respect to ATP as substrate. The isoquinoline inhibitors A1-A5 are ineffective or very poor inhibitors of wheat embryo Ca(2+)-dependent protein kinase (CDPK) and rat brain Ca(2+)-dependent protein kinase C (PKC), chicken gizzard myosin light chain kinase (MLCK) and potato tuber cyclic nucleotide-binding phosphatase (Pase). E1 is a moderately effective inhibitor of CDPK and PKC (IC50 values 30 and 61 microM, respectively). The bisisoquinoline-1(2H)-one compound B7 inhibits cAK, CDPK, PKC and MLCK (IC50 values 8, 95, 24 and 7 microM, respectively) as does J1 [2-(p-bromophenyl)pyrrolo-[2,3-c]isoquinoline-5(4H)-one] (IC50 values 2, 50, 44 and 7 microM, respectively). The very potent isoquinoline-derived cAK inhibitors found here involve substitution of the N-containing isoquinoline ring system and these inhibitors show high specificity for cAK.
Asunto(s)
Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Isoquinolinas/farmacología , Animales , Pollos , Cinética , Hígado/enzimología , Quinasa de Cadena Ligera de Miosina/antagonistas & inhibidores , Quinasa de Cadena Ligera de Miosina/metabolismo , Ratas , Relación Estructura-ActividadRESUMEN
Three basic proteins, M1, M2A and M2B, that are substrates for plant Ca(2+)-dependent protein kinase (CDPK) were purified from seeds of yellow mustard (Sinapis alba L.) by a protocol involving batchwise chromatography on carboxymethylcellulose (CM52), cation-exchange HPLC on an SP5PW column and reversed-phase HPLC on a C18 column. The complete amino-acid sequences of these proteins have been determined employing Edman sequencing and electrospray ionization mass spectrometry (ESMS) applied to the proteins and their tryptic and chymotryptic fragments. M1 (observed mass 5676.8 +/- 1.0 Da; calculated mass 5677.57 Da), M2A (observed mass 5704.8 +/- 0.8 Da; calculated mass 5704.60 Da) and M2B (observed mass 5839.5 +/- 1.2 Da; calculated mass 5838.78 Da) have been identified as gamma-thionins, which are potent antifungal proteins. M1, M2A and M2B are phosphorylated by plant CDPK on Ser residues, the site of phosphorylation on M2A being S8 as directly confirmed by Edman sequencing and mass spectrometry of the chymotryptically generated phosphopeptide CQRPS(HPO3)GTW11. M1 and M2A have apparent calmodulin (CaM) antagonist activity with IC50 values of 4.8 +/- 1.3 microM and 5.5 +/- 1.5 microM, respectively, for inhibition of CaM-dependent myosin light chain kinase (MLCK). M2A and/or M2B interacts with dansyl-CaM in both the presence and absence of calcium.
Asunto(s)
Planta de la Mostaza/química , Proteínas de Plantas/química , Plantas Medicinales , Proteínas Quinasas/metabolismo , Secuencia de Aminoácidos , Sitios de Unión , Calmodulina/antagonistas & inhibidores , Cromatografía Líquida de Alta Presión , Quimotripsina/metabolismo , Espectrometría de Masas , Datos de Secuencia Molecular , Peso Molecular , Fragmentos de Péptidos/química , Fosforilación , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/farmacología , Análisis de Secuencia , Homología de Secuencia de Aminoácido , Espectrometría de Fluorescencia , Tripsina/metabolismoRESUMEN
Six napin small (S) subunits and six napin large (L) subunits were resolved from the seeds of kohlrabi (Brassica napus var. rapifera) by a procedure involving extraction, batchwise elution from carboxymethylcellulose (CM52) and reverse-phase HPLC after treatment with guanidine hydrochloride and 2-mercaptoethanol. The precise average molecular masses of the ca. 4.5 kDa small subunits and the ca. 10 kDa large subunits were determined by electrospray ionisation mass spectrometry (ESMS). The amino-acid sequences of six small subunits (S1A, S1B, S2, S3A, S3B and S4) were deduced from the ESMS-based masses of tryptic fragments, Edman sequencing and previously published data. The deduced structures were precisely consistent with this data and with the ESMS-based average molecular masses of these polypeptides. The structures of the small subunits (39-41 residues) are very similar with variations involving single substitutions at or near the N-terminus and 1 to 3 changes within the last 7 amino acids. Particular B. napus small and large chains are phosphorylated by plant Ca2+-dependent protein kinase (CDPK). The best site of phosphorylation on small chains is inferred to be either S34 or S39 of S1B. The napin-containing basic protein fraction from B. napus seeds largely abolishes the Ca2+-dependent fluorescence enhancement of dansyl-calmodulin and also inhibits calmodulin (CaM)-dependent myosin light chain kinase (MLCK). The resolved napin small chains also inhibit MLCK. All of the kohlrabi napin small chains, as well as homologous Brassicaceae small chains, have a central 23 amino-acid sequence that can potentially form an alpha-helix in which all the basic residues are located on one side. This structural element may be involved in the interaction of these proteins with CaM and the biological activity of antifungal proteins of this kind.
Asunto(s)
Brassica/química , Calmodulina/antagonistas & inhibidores , Proteínas de Plantas/química , Proteínas de Plantas/aislamiento & purificación , Proteínas Quinasas/metabolismo , Albuminas 2S de Plantas , Secuencia de Aminoácidos , Variación Genética , Datos de Secuencia Molecular , Peso Molecular , Quinasa de Cadena Ligera de Miosina/antagonistas & inhibidores , Fragmentos de Péptidos/química , Fosforilación , Estructura Secundaria de Proteína , Semillas/química , Análisis de SecuenciaRESUMEN
Six napin large (L) chains (as well as six napin small chains) were resolved from the seeds of kohlrabi (Brassica napus var. rapifera) by a procedure involving extraction, batchwise elution from carboxymethylcellulose (CM52) and reversed-phase HPLC after treatment with guanidine hydrochloride and 2-mercaptoethanol. The precise average molecular masses of the circa 4.5 kDa small subunits and the circa 10 kDa large subunits were determined by electrospray ionisation mass spectrometry (ESMS). Of six large subunits resolved (L1A), L1B, L1C, L2A, L2B and L2C), the complete amino acid sequences of four (L1A, L2A, L2B and L2C) and the near-complete sequences of two (L1B and L1C) were deduced from the ESMS-based masses of tryptic fragments, Edman sequencing and previously published data. The deduced structures are precisely consistent with this data and with the ESMS-based average molecular masses of these polypeptides. ESMS analysis of unreduced napin extract revealed only seven circa 14.5 kDa complexes, the observed masses being in close agreement with those calculated for 1:1 complexes of particular small and large subunits assuming four disulfides in each napin complex. The structures of the napin large subunits (86-91 residues) are very similar and all amino acid differences observed are confined to only 25 positions. The L2A, L2B AND L2C large chains (but not the L1A, L1B and L1C large chains) are phosphorylated well by plant Ca2+-dependent protein kinase (CDPK). The CDPK-catalyzed phosphorylation site on the large chain L2A is inferred to be S57 within the sequence LQQVIS57RIYQT (the site being S60 within the same sequence in L2B and L2C). The napin-containing basic protein fraction from B. napus seeds largely abolishes the Ca2+-dependent fluorescence enhancement of dansyl-calmodulin and also inhibits calmodulin (CaM)-dependent myosin light chain kinase (MLCK). The resolved napin long chains also inhibit MLCK. Each kohlrabi large chain contains 2 sequences (corresponding to L(10)-Q(20) and Q(51)-L(64) of L1A) which have the potential to form amphipathic alpha-helices. Each large chain also contains a Q-rich 19 amino acid sequence (corresponding to L(30)-Q(48) of L1A) which has the potential to form a '2-sided' alpha-helix with basic residues confined to one side. These structural elements may be involved in the inferred interaction of these proteins with CaM and may be relevant to the biological activity of antifungal proteins of this kind.
Asunto(s)
Brassica/química , Calmodulina/antagonistas & inhibidores , Proteínas de Plantas/química , Proteínas de Plantas/aislamiento & purificación , Proteínas Quinasas/metabolismo , Albuminas 2S de Plantas , Secuencia de Aminoácidos , Datos de Secuencia Molecular , Peso Molecular , Quinasa de Cadena Ligera de Miosina/metabolismo , Fragmentos de Péptidos/química , Fosforilación , Estructura Secundaria de Proteína , Semillas/química , Análisis de SecuenciaRESUMEN
Curcumin [diferuloylmethane; 1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione], a major bioactive secondary metabolite found in the rhizomes of turmeric (Curcuma longa), is an inhibitor of Ca(2+)- and phospholipid-dependent protein kinase C (PKC) and of the catalytic subunit (cAK) of cyclic AMP-dependent protein kinase (IC50 values 15 and 4.8 microM, respectively). Curcumin inhibits plant Ca(2+)-dependent protein kinase (CDPK) (IC50 41 microM), but does not inhibit myosin light chain kinase or a high affinity 3',5'-cyclic AMP-binding phosphatase. Curcumin inhibits cAK, PKC and CDPK in a fashion that is competitive with respect to both ATP and the synthetic peptide substrate employed. The IC50 values for inhibition of cAK by curcumin are very similar when measured with kemptide (LRRASLG) (in the presence or absence of ovalbumin) or with casein or histone III-S as substrates. However, the presence of bovine serum albumin (0.8 mg ml-1) largely overcomes inhibition of cAK by curcumin.
Asunto(s)
Curcumina/farmacología , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Inhibidores de Proteínas Quinasas , Animales , Caseínas , Bovinos , Histonas , Cinética , Oligopéptidos/farmacología , Ovalbúmina/farmacología , Plantas/enzimología , Proteína Quinasa C/antagonistas & inhibidores , Albúmina Sérica Bovina/farmacología , Especificidad por SustratoRESUMEN
The teratogenic metabolite secalonic acid D deriving from the ergot-producing, rye-infecting ascomycete fungus Claviceps purpurea and from Penicillum oxalicum is an inhibitor of Ca2+- and phospholipid-dependent protein kinase C (PKC) and of the catalytic subunit of cyclic AMP-dependent protein kinase (cAK) (C50 values 15 microM and 12 microM, respectively). Secalonic acid D also inhibits Ca2+-calmodulin-dependent myosin light chain kinase (MLCK) and plant Ca2+-dependent protein kinase (CDPK). The inhibition of cAK by secalonic acid D is competitive with respect to both peptide substrate and ATP. However, secalonic acid D does not inhibit a high-affinity nucleotide-binding phosphatase from potato. A variety of other naturally-occurring teratogenic agents are not inhibitors of the protein kinases examined.
Asunto(s)
Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Micotoxinas/farmacología , Proteína Quinasa C/antagonistas & inhibidores , Inhibidores de Proteínas Quinasas , Teratógenos/farmacología , Xantenos/farmacología , Xantonas , Secuencia de Aminoácidos , Animales , Encéfalo/enzimología , Pollos , Claviceps , Inhibidores Enzimáticos/aislamiento & purificación , Molleja de las Aves , Cinética , Datos de Secuencia Molecular , Micotoxinas/aislamiento & purificación , Quinasa de Cadena Ligera de Miosina/antagonistas & inhibidores , Oligopéptidos/farmacología , Penicillium , Fragmentos de Péptidos/síntesis química , Plantas , Ratas , Teratógenos/aislamiento & purificación , Xantenos/aislamiento & purificaciónRESUMEN
A set of plant- and animal-derived amphiphilic triterpenoids have been shown to be potent and selective inhibitors of the catalytic subunit of rat liver cyclic AMP-dependent protein kinase (cAK). Thus plant-derived 18 alpha- and 18 beta-glycyrrhetinic acid, ursolic acid, oleanolic acid and betulin and animal-derived lithocholic acid, 5-cholenic acid and lithocholic acid methyl ester are inhibitors of cAK with IC50 values (concentrations for 50% inhibition) in the range 4-20 microM. These compounds are ineffective or relatively ineffective as inhibitors of various other eukaryote signal-regulated protein kinases namely wheat embryo Ca(2+)-dependent protein kinase (CDPK), avian calmodulin-dependent myosin light chain kinase (MLCK) and rat brain Ca(2+)- and phospholipid-dependent protein kinase C (PKC). These naturally occurring triterpenoids have a common structural motif involving polar residues located at opposite ends of an otherwise non-polar triterpenoid nucleus. A variety of triterpenoids not possessing this structural motif are relatively inactive as inhibitors of cAK and of CDPK, PKC and MLCK. The terpenoid amphiphilic compound crocetin is also a potent and relatively selective inhibitor of cAK (IC50 value for cAK 3.0 microM). 12-Hydroxystearic acid and 10-hydroxydecanoic acid do not inhibit CDPK, PKC or MLCK but are selective inhibitors of cAK (IC50 values 127 and 138 microM, respectively), consistent with a simple model for amphiphile inhibition of cAK involving two polar groups separated by a non-polar region. However, laurylgallate and 15-pentadecanolide are also potent and selective inhibitors of cAK (IC50 values 1.5 and 20 microM, respectively) although the structures of both of these compounds involve a large non-polar portion associated with only one polar region. Crocetin and the plant-derived amphiphilic triterpenoids described here are the most potent non-aromatic plant-derived inhibitors of cAK yet found.
Asunto(s)
Encéfalo/enzimología , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Triterpenos/farmacología , Secuencia de Aminoácidos , Animales , Receptores ErbB/química , Receptores ErbB/metabolismo , Cinética , Datos de Secuencia Molecular , Oligopéptidos/síntesis química , Oligopéptidos/química , Oligopéptidos/metabolismo , Ratas , Relación Estructura-ActividadRESUMEN
There is substantial evidence that protein kinases, through the phosphorylation of substrate proteins, play a significant role in information processing in the brain, including processes underlying memory formation. Inhibition of the activity of the cyclic-adenosine monophosphate-dependent protein kinase A by the highly specific inhibitor, halofantrine, resulted in impairment of memory formation in day-old chicks trained on a single-trial passive avoidance task. A dose of 9.6 ng/chick halofantrine induced amnesia at the beginning of a protein synthesis-dependent long-term memory stage, the last of three stages of memory postulated to underly memory formation in the chick following passive avoidance learning. The concentration of halofantrine required for 50% inhibition of chick brain protein kinase A was found to be similar to that observed for bovine heart and rat liver. The amnestic effect of halofantrine is tentatively attributed to interference with de novo protein synthesis necessary for long-term memory consolidation. Neither anthraquinone nor the anthraquinone derivative anthraflavic acid, which have little effect on protein kinase A activity, affected memory retention. On the other hand, two other anthraquinone derivatives, chrysophanic acid and purpurin, which inhibit PKA activity, at doses of 0.25 and 0.5 ng/chick also yielded retention deficits. In these cases, however, retention losses occurred earlier than observed with halofantrine, at about 30 min post-training. The earlier effects of these inhibitors may be due to the additional inhibitory action of these compounds on protein kinase C activity, which has been demonstrated in previous studies to be implicated, possibly through phosphorylation of the GAP43 phosphoprotein, in memory processing in the stage of memory immediately preceding the protein synthesis-dependent long-term stage.
Asunto(s)
Reacción de Prevención/efectos de los fármacos , Condicionamiento Clásico/efectos de los fármacos , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Fenantrenos/farmacología , Retención en Psicología/efectos de los fármacos , Animales , Reacción de Prevención/fisiología , Encéfalo/efectos de los fármacos , Encéfalo/fisiología , Pollos , Condicionamiento Clásico/fisiología , Proteínas Quinasas Dependientes de AMP Cíclico/fisiología , Relación Dosis-Respuesta a Droga , Recuerdo Mental/efectos de los fármacos , Recuerdo Mental/fisiología , Retención en Psicología/fisiologíaRESUMEN
A heat-stable wheat protein (WP) that is a good substrate for wheat embryo Ca(2+)-dependent protein kinase (CDPK) was purified from wheat embryo by a procedure involving batchwise anion exchange chromatography on DEAE-cellulose (DE52), passage through Phenyl-Sepharose CL-4B, heat and acid treatment and anion exchange HPLC on a DEAE-5PW column. WP is phosphorylated by CDPK to a stoichiometry of about 0.8 mol phosphoryl per mol WP. The Km for WP is 3.5 microM. WP is phosphorylated by CDPK on Ser residues. [32P]phosphoWP exactly copurifies on SDS-PAGE with WP (59 kDa). Phosphorylation of WP by CDPK is largely Ca(2+)-dependent. The N-terminal amino acid sequence of WP has homology with bacterial azurins. Evidence for two serine phosphorylation sites was obtained from sequencing of phosphopeptides derived from tryptic and chymotryptic digests of phosphoWP. One major site of phosphorylation is inferred to be on a serine within the sequence KKMASMK. WP is one of the best endogenous protein substrates yet found for wheat embryo CDPK. A 59kDa protein is phosphorylated in vivo in sprouting wheat.
Asunto(s)
Calcio/farmacología , Fosfoproteínas/aislamiento & purificación , Proteínas de Plantas/aislamiento & purificación , Proteínas Quinasas/metabolismo , Semillas/enzimología , Triticum/química , Secuencia de Aminoácidos , Azurina/química , Sitios de Unión , Quimotripsina/metabolismo , Estabilidad de Medicamentos , Calor , Datos de Secuencia Molecular , Fragmentos de Péptidos/metabolismo , Fosfoproteínas/química , Fosfoproteínas/metabolismo , Fosfoserina/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Homología de Secuencia , Especificidad por Sustrato , Triticum/embriologíaRESUMEN
A variety of hydrolysable tannins purified from Phyllanthus amarus are potent inhibitors of rat liver cyclic AMP-dependent protein kinase catalytic subunit (cAK) with IC50 values (concentrations for 50% inhibition) in the range 0.2-1.7 microM. The three most effective compounds of this series of hydrolysable tannins have five phenolic substituents. These three compounds are also the most effective inhibitors of wheat embryo Ca(2+)-dependent protein kinase (CDPK), rat brain Ca(2+)- and phospholipid-dependent protein kinase C (PKC) and Ca(2+)-calmodulin-dependent myosin light chain kinase (MLCK). The order of sensitivity for protein kinase inhibition by the hydrolysable tannins studied is cAK > CDPK > PKC > MLCK. Thus the IC50 values for protein kinase inhibition by the most potent compound are 0.2 microM (for cAK), 1.8 microM (for CDPK), 26 microM (for PKC) and 56 microM (for MLCK) when protein kinase affinity is measured using synthetic peptide substrates. These hydrolysable tannin inhibitors found are the most specific and potent plant-derived inhibitors of cAK yet found.
Asunto(s)
Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Quinasa de Cadena Ligera de Miosina/antagonistas & inhibidores , Proteína Quinasa C/antagonistas & inhibidores , Transducción de Señal , Taninos/farmacología , Secuencia de Aminoácidos , Animales , Pollos , Molleja de las Aves/enzimología , Hidrólisis , Hígado/enzimología , Datos de Secuencia Molecular , Ratas , Especificidad por Sustrato , Taninos/metabolismoRESUMEN
Four low molecular weight, basic proteins (WBP1A, WBP1B, WBP2 and WBP3) that are substrates for wheat germ Ca(2+)-dependent protein kinase (CDPK) were purified from wheat germ by a procedure involving batchwise cation exchange on carboxymethylcellulose (CM52), acid precipitation, cation exchange HPLC on an SP5PW column and reverse-phase HPLC on a C18 column. While WBP1A, WBP1B and WBP3 are phosphorylated by wheat germ CDPK exclusively on Ser residues, WBP2 is phosphorylated on both Ser and Thr residues. CDPK-catalysed phosphorylation sites on WBP1A and WBP1B were determined. With all four proteins the phosphorylated form comigrates with non-phosphorylated protein (Mr about 9 kDa) on SDS-PAGE. Average molecular masses of reduced WBP1A, WBP1B, WBP2 and WBP3 measured using electrospray ionisation mass spectrometry (ESMS) are 9389 Da, 9274 Da, 9479 Da and 9467 Da, respectively. The complete amino-acid sequences of WBP1A and WBP1B (determined by Edman sequencing and ESMS of proteolytically derived fragments) and N-terminal sequences of WBP2 and WBP3 are highly homologous to each other and to sequences of low molecular weight, basic plant lipid transfer proteins (LTPs).
Asunto(s)
Proteínas Portadoras/aislamiento & purificación , Proteínas de Plantas/aislamiento & purificación , Triticum/química , Secuencia de Aminoácidos , Antígenos de Plantas , Proteínas Portadoras/química , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Fosforilación , Proteínas de Plantas/química , Proteínas Quinasas/química , Triticum/enzimologíaRESUMEN
The phenanthrenemethanol antimalarial halofantrine is a potent inhibitor of bovine heart and rat liver cyclic AMP-dependent protein kinase catalytic subunit (cAK) (IC50 values 2.1 microM and 0.6 microM, respectively). The inhibition of rat liver cAK by halofantrine is non-competitive with respect to both ATP and to the synthetic peptide substrate employed (LRRASLG). Halofantrine is a poor inhibitor of calmodulin-dependent myosin light chain kinase (MLCK) and wheat embryo Ca(2+)-dependent protein kinase (CDPK) and does not inhibit rat brain Ca(2+)- and phospholipid-dependent protein kinase C (PKC). In contrast, the acridine-based antimalarial quinacrine and a variety of quinoline-based antimalarials are very poor inhibitors of cAK, the best inhibitor being chloroquine (IC50 for bovine heart cAK, 80 microM). Quinacrine and the quinoline-based antimalarials variously inhibit CDPK, PKC and MLCK albeit at relatively high concentrations (about 1 to 4 x 10(-4) M), the best inhibitors found being primaquine, pentaquine and mefloquine (IC50 values for MLCK 49, 103 and 33 microM, respectively). A number of phenanthrene derivatives having a 9-hydroxy or 9-keto substituent, namely phenanthrenequinone, 6(5H)-phenanthridinone and 9-phenanthrol are potent inhibitors of bovine heart cAK (IC50 values 8, 10 and 10 microM, respectively) and of MLCK (IC50 values 6, 53 and 10 microM, respectively). The selective, high affinity interaction of halofantrine with cAK may contribute to biological effects in vivo of this clinically-employed antimalarial compound.