Computational search for UV radiation resistance strategies in Deinococcus swuensis isolated from Paramo ecosystems.

Ultraviolet radiation (UVR) is widely known as deleterious for many organisms since it can cause damage to biomolecules either directly or indirectly via the formation of reactive oxygen species. The goal of this study was to analyze the capacity of high-mountain Espeletia hartwegiana plant phyllosphere microorganisms to survive UVR and to identify genes related to resistance strategies. A strain of Deinococcus swuensis showed a high survival rate of up to 60% after UVR treatment at 800J/m2 and was used for differential expression analysis using RNA-seq after exposing cells to 400J/m2 of UVR (with >95% survival rate). Differentially expressed genes were identified using the R-Bioconductor package NOISeq and compared with other reported resistance strategies reported for this genus. Genes identified as being overexpressed included transcriptional regulators and genes involved in protection against damage by UVR. Non-coding (nc)RNAs were also differentially expressed, some of which have not been previously implicated. This study characterized the immediate radiation response of D. swuensis and indicates the involvement of ncRNAs in the adaptation to extreme environmental conditions.

Reconstrucción traqueal bajo oxigenación con membrana para circulación extracorpórea para el manejo de una lesión traumática: reporte de caso y revisión bibliográfica / Tracheal reconstruction under oxygenation with membrane extracorporeal circulation for the management of a traumatic lesion: case report and literature

Introducción. El traumatismo traqueal es una condición poco frecuente que puede ser ocasionada por traumas abiertos, cerrados o iatrogénicos; su presentación clínica es variable y el diagnóstico suele ser clínico, apoyándose en la radiografía de tórax, la tomografía cérvico-torácica y la fibrobroncoscopia. Su manejo representa todo un reto médico y quirúrgico, y se requieren múltiples herramientas para su adecuado tratamiento. La terapia con oxigenación con membrana para circulación extracorpórea ha sido ampliamente utilizada en el manejo de pacientes con falla respiratoria aguda, en los cuales los métodos convencionales de asistencia respiratoria mecánica no son suficientes para garantizar una adecuada oxigenación. Caso clínico. Se presenta el caso de una paciente con una lesión traqueal iatrogénica reparada quirúrgicamente, bajo asistencia con oxigenación con membrana para circulación extracorpórea, para garantizar la oxigenación tisular y la adecuada recuperación y supervivencia de la paciente. Conclusiones. La terapia con oxigenación con membrana para circulación extracorpórea es una excelente alternativa para el manejo quirúrgico de las lesiones traqueales complejas que amenazan la vida del paciente, ya que permite brindar un soporte vital y un adecuado intercambio gaseoso durante el procedimiento

ntroduction: Tracheal trauma is a rare condition that can be caused by open, closed, or iatrogenic trauma; its clinical presentation is variable and the diagnosis is usually clinical, supported by chest X-ray, thoracic CT and bronchoscopy. Its management represents a medical and surgical challenge, requiring multiple tools for the proper treatment of this entity. Extracorporeal membrane oxygenation therapy has been widely used in the management of patients with acute ventilatory failure in whom conventional methods of mechanical ventilation are insufficient to ensure adequate oxygenation of the patient. Case report: We present the case of a patient with a surgically repaired iatrogenic tracheal lesion conducted with extracorporeal membrane oxygenation to maintain tissue oxygenation and assure recuperation and survival. Conclusions: Extracorporeal membrane oxygenation therapy is an excellent alternative for the surgical management of complex tracheal lesions that threaten the patient's life, allowing vital support and adequate gas exchange during the procedure

Klebsiella pneumoniae yfiRNB operon affects biofilm formation, polysaccharide production and drug susceptibility.

Klebsiella pneumoniae is an opportunistic pathogen important in hospital-acquired infections, which are complicated by the rise of drug-resistant strains and the capacity of cells to adhere to surfaces and form biofilms. In this work, we carried out an analysis of the genes in the K. pneumoniae yfiRNB operon, previously implicated in biofilm formation. The results indicated that in addition to the previously reported effect on type 3 fimbriae expression, this operon also affected biofilm formation due to changes in cellulose as part of the extracellular matrix. Deletion of yfiR resulted in enhanced biofilm formation and an altered colony phenotype indicative of cellulose overproduction when grown on solid indicator media. Extraction of polysaccharides and treatment with cellulase were consistent with the presence of cellulose in biofilms. The enhanced cellulose production did not, however, correlate with virulence as assessed using a Caenorhabditis elegans assay. In addition, cells bearing mutations in genes of the yfiRNB operon varied with respect to the WT control in terms of susceptibility to the antibiotics amikacin, ciprofloxacin, imipenem and meropenem. These results indicated that the yfiRNB operon is implicated in the production of exopolysaccharides that alter cell surface characteristics and the capacity to form biofilms--a phenotype that does not necessarily correlate with properties related with survival, such as resistance to antibiotics.

Disautonomía: presentación de una serie de casos manejados con repolarizador magnético celular / Dysautonomia: serial cases managed with magnectic cellular repolarized system

Objetivo: describir el comportamiento de la disautonomía mediante su tratamiento con terapias complementarias como la medicina alternativa con repolarizador magnético celular (RMC). Metodología: estudio de serie de casos en pacientes disautonómicas en quienes se empleó el RMC, en quienes se analizaron características demográficas, presencia de síncopes y de síntomas presincopales, cambios en las cifras de presión arterial y frecuencia cardiaca, y resultados de calidad de vida (se utilizaron 2 cuestionarios SF36 y EQ-5D y se agruparon las pacientes en 4 grupos de acuerdo con las puntuaciones). Resultados: se encontró similitud con otras series en cuanto a datos demográficos, predominio y severidad en los síntomas en el sexo femenino con edades entre 16 y 25 años, además de pesos ligeros. Todas las pacientes presentaron pródromos en algún momento de su enfermedad, mientras los síncopes se presentaron en el 57%. En la presión arterial 6/7 presentaron hipotensión y la frecuencia cardiaca tuvo un promedio de 70 latidos/minuto y 80 latidos/minuto en el inicio y en el seguimiento, respectivamente. En los puntajes se presentó mejoría de la calidad de vida, si se compara el seguimiento con el inicio. Conclusiones: la disautonomía afecta más a mujeres jóvenes y delgadas, está relacionada con regular a mala calidad de vida, pero ésta mejora sustancialmente con tratamientos bien instaurados, como aumento en la ingesta de sal y líquidos y con maniobras de contrapresión para mejorar el retorno vascular. También se observó mejoría con el RMC.

Objective: to describe the behavior of disautonomia through its management with complementary therapies like alternative medicine with magnetic cellular repolarized system (MCRS).Methods: case study done in disautonomic patients that were managed with MCRS, in whom the demographic characteristics, the presence of syncopes and presyncopal symptoms, changes in the blood pressure and heart rate, and the results of quality of life were analyzed (two questionnaires were used, SF36 and EQ-5D, and the patients were grouped in four groups according to the punctuations).Results: there were similarities found with other series in regards to demographic data, finding a prevalence and severity in the female symptoms with ages between 16 and 25 years old, in addition to light weight. All of the patients presented prodromes sometime during their disease, while the syncopes were presented by 57% of them. In blood pressure 6/7 presented hypotension and the heart rate had an average of 70 beats per minute and 80 beats per minute at the beginning and follow up respectively. In the punctuation there was improvement of the quality of life, comparing the follow up with the beginning. Conclusions: disautonomia affects more young and thin women, it is related to regular and bad quality of life, but it gets better with well-established treatment, basic measures such as the increase of salt and liquid consumption and compression maneuver to improve the vascular return, improvement was also seen with MCRS.