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INTRODUCTION: The COVID-19 pandemic has created a high demand on personal protective equipment, including disposable N95 masks. Given the need for mask reuse, we tested the feasibility of vaporized hydrogen peroxide (VHP), ultraviolet light (UV), and ethanol decontamination strategies on N95 mask integrity and the ability to remove the infectious potential of SARS-CoV-2. METHODS: Disposable N95 masks, including medical grade (1860, 1870+) and industrial grade (8511) masks, were treated by VHP, UV, and ethanol decontamination. Mask degradation was tested using a quantitative respirator fit testing. Pooled clinical samples of SARS-CoV-2 were applied to mask samples, treated, and then either sent immediately for real-time reverse transcriptase-polymerase chain reaction (RT-PCR) or incubated with Vero E6 cells to assess for virucidal effect. RESULTS: Both ethanol and UV decontamination showed functional degradation to different degrees while VHP treatment showed no significant change after two treatments. We also report a single SARS-CoV-2 virucidal experiment using Vero E6 cell infection in which only ethanol treatment eliminated detectable SARS-CoV-2 RNA. CONCLUSIONS: We hope our data will guide further research for evidenced-based decisions for disposable N95 mask reuse and help protect caregivers from SARS-CoV-2 and other pathogens.
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Reconstruction of an image (or shape or wavefront) from measurements of the derivatives of the image in two orthogonal directions is a common problem. We demonstrate how a particular reconstructor, commonly referred to as the Fried algorithm, can be used with megapixel derivative images to recover the original image. Large datasets are handled by breaking the derivative images into smaller tiles, applying the Fried algorithm and stitching the tiles back together. The performance of the algorithm is demonstrated using differential interference contrast microscopy on a known test object.
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Algoritmos , Procesamiento de Imagen Asistido por Computador/métodos , Microscopía/métodos , Fenómenos ÓpticosRESUMEN
INTRODUCTION: We present a clinically relevant method for producing and sterilizing dissolvable albumin stents to provide intraluminal support during vascular anastomosis, and a method for photothermally soldering vessels using a 1.9 µm diode laser with a 200-µm spot size, albumin solder, and water as the chromophore. Our aim in this study was to assess the mechanical integrity of soldered vessels, and to determine if gamma-irradiation affected the solubility of the stents. MATERIALS AND METHODS: The axial tensile strength and burst pressure of 3.75 ± 0.3 mm inner-diameter vessels soldered with varied swath width (1-7 mm), laser power (430-610 mW), solder concentration (22-46%w/w), and solder layering (1-3 layers) was tested in vitro. Stent dissolution was monitored by weight in blood, and with UV absorbance measurements in phosphate buffered saline (PBS). Solubility was measured for stents sterilized by 25 kGy gamma-irradiation, and stents with varied diameter and wall thickness. RESULTS: Optimized soldering parameters yielded tensile strengths of 4.4 ± 1.2 N and burst pressures of 400 ± 90 mm Hg with stay sutures. Differences in stent solubility in blood and PBS were not statistically significant (p = 0.99). Sterilization by 25 kGy gamma-irradiation did not cause significant changes (p > 0.6) in stent solubility, which was primarily volume-dependent. Under simulated intravascular flow conditions, 3 mm stents dissolved completely with 2.7 ± 0.7 ml/mg. CONCLUSIONS: The results show that fast-dissolving stents can be produced reliably using the extrusion technique, and sterilized by gamma-irradiation. Without stay sutures, soldered vessels exhibited low tensile strength, but burst pressures comparable to sutured vessels. It was concluded that stay sutures would be necessary in vivo due to degradation of the tensile strength of soldered vessels with exposure to moisture.
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Albúminas , Materiales Biocompatibles , Láseres de Semiconductores , Stents , Injerto Vascular/instrumentación , Anastomosis Quirúrgica , Animales , Arterias Carótidas/fisiología , Arterias Carótidas/cirugía , Humanos , Técnicas In Vitro , Presión , Esterilización , Porcinos , Resistencia a la Tracción , Injerto Vascular/métodosRESUMEN
OBJECTIVES: Since photoinitiator systems for dental resins based on camphorquinone (CQ) present color disadvantages, trimethylbenzoyl-diphenyl-phosphine oxide (TPO) has been proposed as an alternative. However, there are remaining considerations about its curing efficiency. The aims of the present investigation were: to characterize the relationship between the photoinitiator absorption spectra and the light spectrum emitted from a QTH light (absorbed power density, PD(abs)); to evaluate the kinetics of polymerization, and the depth of cure for filled dimethacrylate resins formulated with different photoinitiator systems. METHODS: CQ+EDMAB (control); TPO and TPO+EDMAB were used in 50:50 Bis-GMA/TEGDMA resins. Photoinitiator absorption and QTH-light emission were evaluated using a spectrophotometer and kinetics of polymerization with differential scanning calorimetry (DSC) (n=3). Depth of cure was analyzed by the scraping method (n=5), as recommended by ISO4049. One-way ANOVA/Tukey's (p<0.05) was used to analyze the results. RESULTS: CQ presented higher PD(abs) than TPO (364 and 223 mW/cm(3), respectively). The DSC revealed that TPO and TPO+EDMAB produced a faster reaction than CQ+EDMAB. Composite formulated with CQ+EDMAB produced higher depth of cure (6.3±0.4 mm) than those with TPO (4.3±0.1) or TPO+EDMAB (4.2±0.3). SIGNIFICANCE: Although CQ presented higher PD(abs) than TPO, formulations containing TPO exhibited higher reactivity than that with CQ. On the other hand, materials formulated with TPO demonstrated lower depth of cure than that with CQ. Therefore, its use as an alternative photoinitiator requires further investigation, with higher concentrations.
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Resinas Compuestas/química , Luces de Curación Dental , Curación por Luz de Adhesivos Dentales , Fotoiniciadores Dentales , Rastreo Diferencial de Calorimetría , Dureza , Cinética , Ensayo de Materiales , Fosfinas , Polimerizacion , Espectrofotometría , TerpenosRESUMEN
We present a method of using an unmodified differential interference contrast microscope to acquire quantitative information on scatter and absorption of thin tissue samples. A simple calibration process is discussed that uses a standard optical wedge. Subsequently, we present a phase-stepping procedure for acquiring phase gradient information exclusive of absorption effects. The procedure results in two-dimensional maps of the local angular (polar and azimuthal) ray deviation. We demonstrate the calibration process, discuss details of the phase-stepping algorithm, and present representative results for a porcine skin sample.
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Microscopía/métodos , Fenómenos Ópticos , Animales , Piel/citología , PorcinosRESUMEN
We present the design and calibration of a spectroscopic sensitive polarimeter. The polarimeter can measure the full Stokes vector in the wavelength range 550 to 750 nm with 1-nm resolution and consists of a fiber-based spectrophotometer, a white light emitting diode light source, two liquid crystal retarders, and one polarizer. Calibration of the system is achieved with a scheme that does not require knowledge of the polarizing elements' orientation or retardation. Six intensity spectra are required to calculate the full spectrum Stokes vector. Error in the polarimeter is less than 5%. We report the Stokes vectors for light transmitted through nonscattering polarizing elements as well as a measurement of the depolarizing properties of chicken muscle at several wavelengths.
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Óptica y Fotónica/instrumentación , Análisis Espectral/instrumentación , Algoritmos , Animales , Calibración , Pollos , Diagnóstico por Imagen , Diseño de Equipo , Modelos Teóricos , Músculos/química , Análisis Espectral/métodosRESUMEN
BACKGROUND: Bleeding from the liver surface is common after hepatic resection. Animal studies have demonstrated superiority of argon beam coagulation (ABC) and 38% human serum albumin when applied together after partial liver resection when compared to ABC alone. There are no data addressing the combination of albumin and argon beam coagulation (ABCA) applied to the bleeding liver after resection in humans. The aim of this study was to evaluate the safety and efficacy of ABCA on hemostasis when applied to the surface of the liver remnant post-hepatic resection. METHODS: Ten patients underwent liver resection and were treated with ABCA immediately after the liver was divided. The liver surface was coated with albumin and ABC applied simultaneously, the liver was covered with gauze for 3 min, and ABCA was repeated if necessary. Number of rebleeding episodes requiring re-application of ABCA, time of ABCA application, overall blood loss, and liver functions were monitored. Patients were followed for at least 6 months. RESULTS: Nine of 10 patients required a single application of ABCA, and one patient required two treatments. Average time of ABC use was 5 ± 3 min. Median blood loss was 230 ml. Liver functions returned to near normal within 4 days of resection. CONCLUSIONS: ABCA performed well with respect to hemostatic properties, much like previous observations in animal studies. Further clinical trials are justified using this technique.
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Coagulación con Plasma de Argón , Hemostasis Quirúrgica/métodos , Hepatectomía , Albúmina Sérica/administración & dosificación , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
We present a Monte Carlo-derived Green's function for the propagation of partially spatially coherent fields. This Green's function, which is derived by sampling Huygens-Fresnel wavelets, can be used to propagate fields through an optical system and to compute first- and second-order field statistics directly. The concept is illustrated for a cylindrical f/1 imaging system. A Gaussian copula is used to synthesize realizations of a Gaussian Schell-model field in the pupil plane. Physical optics and Monte Carlo predictions are made for the first- and second-order statistics of the field in the vicinity of the focal plane for a variety of source coherence conditions. Excellent agreement between the physical optics and Monte Carlo predictions is demonstrated in all cases. This formalism can be generally employed to treat the interaction of partially coherent fields with diffracting structures.
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Luz , Método de Montecarlo , Absorción , Simulación por Computador , Modelos Químicos , Dispersión de RadiaciónRESUMEN
We present a Monte Carlo method for propagating partially coherent fields through complex deterministic optical systems. A Gaussian copula is used to synthesize a random source with an arbitrary spatial coherence function. Physical optics and Monte Carlo predictions of the first- and second-order statistics of the field are shown for coherent and partially coherent sources for free-space propagation, imaging using a binary Fresnel zone plate, and propagation through a limiting aperture. Excellent agreement between the physical optics and Monte Carlo predictions is demonstrated in all cases. Convergence criteria are presented for judging the quality of the Monte Carlo predictions.
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Modelos Estadísticos , Modelos Teóricos , Refractometría/métodos , Simulación por Computador , Luz , Método de Montecarlo , Dispersión de RadiaciónRESUMEN
OBJECTIVES: To evaluate the degree of conversion (DC), maximum rate of polymerization (Rpmax), Knoop hardness (KHN) and yellowing (b-value) of resin composites formulated with phenylpropanedione (PPD), camphorquinone (CQ), or CQ/PPD at different concentrations. The hypotheses tested were (i) PPD or CQ/PPD would produce less Rpmax and yellowing than CQ alone without affecting DC and KHN, and (ii) Rpmax, DC, and KHN would be directly related to the absorbed power density (PDabs). METHODS: CQ/amine, PPD/amine and CQ/PPD/amine were used at low, intermediate and high concentrations in experimental composites. Photoinitiator absorption and halogen-light emission were measured using a spectrophotometer, Rp with differential scanning calorimetry (DSC), DC with DSC and FTIR, KHN with Knoop indentation; and color with a chromameter. The results were analyzed with two-way analysis of variance (ANOVA)/Student-Newman-Keul's test (p<0.05). Correlation tests were carried out between PDabs and each of DC, Rpmax and KHN. RESULTS: The PDabs increased with photoinitiator concentration and PPD samples had the lowest values. In general, maximum DC was comparable at intermediate concentration, while Rpmax and KHN required higher concentrations. DC was similar for all photoinitiators, but Rpmax was lower with PPD and CQ/PPD. PPD produced the lowest KHN. Yellowing increased with photoinitiator concentration. PPD did not reduce yellowing at intermediate and/or high concentrations, compared to CQ-formulations. PDabs showed significant correlations with DC, Rpmax and KHN. CONCLUSION: PPD or CQ/PPD reduced Rpmax in experimental composites without affecting the DC. The use of PPD did not reduce yellowing, but reduced KHN. DC, Rpmax and KHN were dependent on PDabs.
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Resinas Compuestas/química , Materiales Dentales/química , Ácido 4-Aminobenzoico/química , Ácido 4-Aminobenzoico/efectos de la radiación , Absorción , Bisfenol A Glicidil Metacrilato/química , Bisfenol A Glicidil Metacrilato/efectos de la radiación , Rastreo Diferencial de Calorimetría , Chalconas/química , Chalconas/efectos de la radiación , Color , Resinas Compuestas/efectos de la radiación , Materiales Dentales/efectos de la radiación , Dureza , Humanos , Ensayo de Materiales , Polietilenglicoles/química , Polietilenglicoles/efectos de la radiación , Polímeros/química , Polímeros/efectos de la radiación , Ácidos Polimetacrílicos/química , Ácidos Polimetacrílicos/efectos de la radiación , Dosis de Radiación , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Terpenos/química , Terpenos/efectos de la radiación , para-AminobenzoatosRESUMEN
The primary absorber in dental resins is the photoinitiator, which starts the photo polymerization process. We studied the quantum yield of conversion of camphorquinone (CQ), a blue light photoinitiator, in dental resin composites using a LED lamp (3M FreeLight) and a Quartz Tungsten Halogen (QTH) lamp (VIP) as the light curing units at five different irradiances. The molar extinction coefficient, epsilon(469), of CQ was 46+/-2 cm(-1)/(mol/L) at 469 nm. The reciprocity of irradiance and exposure time holds for changes of CQ absorption coefficient, that is, irradiance x exposure time (=radiant exposure)=constant. Both LED and QTH lamps yielded the same curing threshold (the radiant exposure when CQ absorption drops to 1/e) and the same quantum yield conversion under different irradiances. In our dental resin formulation (0.7 wt.% CQ with reducing agents 0.35 wt.% dimethylaminoethyl methacrylate (DMAEMA) and 0.05 wt.% butylated hydroxytoluene (BHT)) the quantum yield was measured as 0.07+/-0.01 CQ conversion per absorbed photon.
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Resinas Compuestas/efectos de la radiación , Transición de Fase/efectos de la radiación , Radiometría/métodos , Tecnología Odontológica/métodos , Absorción , Bisfenol A Glicidil Metacrilato/efectos de la radiación , Luz , Fotones , Polietilenglicoles/efectos de la radiación , Ácidos Polimetacrílicos/efectos de la radiación , TerpenosRESUMEN
We describe a method for the preparation of a polyurethane phantom to simulate the optical properties of biologic tissues at two wavelengths in the visible and near-infrared spectral range. We characterize the addition of added molecular absorbers with relatively narrow absorption bands [full width at half maximum (FWHM) 32 and 76 nm for Epolight 6084 and 4148, respectively] for independent absorption at 690 nm for absorption up to 5 cm(-1), and 830 nm for absorptions up to 3 cm(-1). Absorption by both dyes is linear with concentration in these respective regions and is consistent in polyurethane both before and after curing. The dyes are stable over long durations with no more than 4% change. The absorption of visible light by polyurethane decreases with time and is stable by one year with a drop of 0.03+/-0.003 cm(-1) from 500 to 830 nm. The scattering properties are selected by the addition of TiO2 particles to the polyurethane, which we functionally describe for the 690- and 830-nm wavelengths as related to the weight per volume. We demonstrate that the variation in absorption and scattering properties for large batch fabrication (12 samples) is +/-3%. The optical properties of the phantoms have not significantly changed in a period of exceeding one year, which makes them suitable for use as a reference standard.
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Materiales Biomiméticos/química , Biomimética/instrumentación , Modelos Biológicos , Fantasmas de Imagen , Poliuretanos/química , Diseño de Equipo , Análisis de Falla de Equipo , HumanosRESUMEN
A molecularly imprinted polymer (MIP) is a biomimetic material that can be used as a biochemical sensing element. We studied the steady-state and time-resolved fluorescence and fluorescence anisotropy of anthracene-imprinted polyurethane. We compared MIPs with imprinted analytes present, MIPs with the imprinted analytes extracted, MIPs with rebound analytes, non-imprinted control polymers (non-MIPs) and non-MIPs bound with analytes to understand MIP's binding behaviour. MIPs and non-MIPs had similar steady-state fluorescence anisotropy in the range 0.11-0.24. Anthracene rebound in MIPs and non-MIPs had a fluorescence lifetime of tau = 0.64 ns and a rotational correlation time of phi(F) = 1.2-1.5 ns, both of which were shorter than that of MIPs with imprinted analytes present (tau = 2.03 ns and phi(F) = 2.7 ns). The steady-state anisotropy of polymer solutions increased exponentially with polymerization time and might be used to characterize the polymerization extent in situ.
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Polarización de Fluorescencia , Polímeros/químicaRESUMEN
OBJECTIVES: The purpose of this study was to build a photo migration model to calculate the radiant exposure (irradiancextime) in dental composite and to relate the radiant exposure with extent of cure using polymer kinetics models. METHODS: A composite (Z100, Shade A2) cylinder (21 mm diameter by 15 mm deep) was cured with a tungsten-halogen lamp emitting 600 mW/cm2, 1 mm above the composite for 60s. For each of the 2x1 mm grids along the longitudinal cross section (diameter versus depth), the degree of conversion (DC) and hardness (KHN) were measured to construct the curing extent distribution. The inverse adding-doubling method was used to characterize the optical properties of the composite for the Monte Carlo model simulating the photon propagation within the composite cylinder. The calculated radiant exposure (H) distribution along the cross section was related to the curing extent DC/DC(max) distribution and fitted with two polymer curing kinetics models, the exponential model DC=DC(max)[1-exp((ln0.5)H/H(dc)(50%))] and Racz's model [Formula: see text] , where H(dc)(50%) is a fitting parameter representing the threshold for 50% of the maximum curing level. RESULTS AND SIGNIFICANCE: The absorption and scattering coefficients of uncured composite were higher than that of cured composite at wavelengths between 420 and 520 nm. A roughly hemi-spheric distribution of radiant exposure in the Monte Carlo simulation result was comparable with the curing profiles determined by both DC and KHN. The relationship between DC (or KHN) and H agreed with the Racz model (r2=0.95) and the exponential model (r2=0.93). The H(dc)(50%) was 1.5(0.1), equal for the two models (P<0.05). The estimated radiant exposure threshold for 80% of the maximum curing level was between 3.8 and 8.8 J/cm2. The simulation results verify that the radiant exposure extends to a greater depth and width for composite with lower absorption and scattering coefficients. Given the optical properties and the geometry of the composite, and the spectrum and the geometry of the light source, the Monte Carlo simulation can accurately describe the radiant exposure distribution in a composite material to predict the extent of cure.
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Resinas Compuestas/efectos de la radiación , Modelos Químicos , Dureza , Cinética , Ensayo de Materiales , Método de Montecarlo , Transición de Fase , Fotones , Proyectos Piloto , Dispersión de Radiación , Dióxido de Silicio/efectos de la radiación , Factores de Tiempo , Circonio/efectos de la radiaciónRESUMEN
Three Monte Carlo programs were developed which keep track of the status of polarization of light traveling through mono-disperse solutions of micro-spheres. These programs were described in detail in our previous article [1]. This paper illustrates a series of Monte Carlo simulations that model common experiments of light transmission and reflection of scattering media. Furthermore the codes were expanded to model light propagating through poly-disperse solutions of micro-spheres of different radii distributions.
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Polarized light imaging has been used to detect the borders of skin cancer and facilitate assessment of cancer boundaries. A design for an inexpensive handheld polarized camera is presented and clinical images acquired with this prototype are shown. The camera is built with two universal serial bus (USB) color video cameras, a polarizing beamsplitter cube, and a 4x objective lens. Illumination is provided by three white LEDs and a sheet polarizer. Horizontal and vertical linearly polarized reflected images are processed at 7 frames/s and a resulting polarized image is displayed on screen. We compare the performances of cheap USB camera and a 16-bit electronically cooled camera. Dark noise and image repeatability are compared. In both cases, the 16-bit camera outperforms the USB cameras. Despite these limitations, the results obtained with this USB prototype are very satisfactory. Examples of polarized images of lesions taken prior to surgery are presented.
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Melanoma/patología , Microscopía de Polarización/instrumentación , Microscopía por Video/instrumentación , Procesamiento de Señales Asistido por Computador/instrumentación , Neoplasias Cutáneas/patología , Grabación en Video/instrumentación , Redes de Comunicación de Computadores/instrumentación , Diseño de Equipo , Análisis de Falla de Equipo , Humanos , Microscopía de Polarización/métodos , Microscopía por Video/métodos , Miniaturización , Fotograbar/instrumentación , Fotograbar/métodos , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Grabación en Video/métodosRESUMEN
Topically applied concentrated albumin with argon beam coagulation (ABCA) has been shown to be more effective at achieving hemostasis than using argon beam coagulation alone (ABC) in a liver injury model. This study investigated the host response to the concentrated albumin after argon beam coagulation. Complete hemostasis was achieved using ABCA (N = 10) or ABC (N = 10) on a nonanatomic liver resection in a heparinized porcine model. The repairs were evaluated grossly and microscopically at postoperative periods of 30 and 90 days. We found no evidence of biliary leakage, rebleeding, or intraabdominal infection. Blood analysis indicated liver chemistry indices were within normal range after ABC and ABCA treatments. The histopathology showed that the postoperative healing response was similar in both groups: a moderate chronic inflammatory response as part of an on-going normal healing process. All repairs were encapsulated by fibrous tissue. There was no difference in the postsurgical adhesion scores for the ABCA (mean 3.4) and ABC (mean 3.8). It was concluded that use of the concentrated albumin as biological glue in conjunction with argon beam coagulation is a safe and efficient procedure for controlling hepatic hemorrhage in surgery. Further studies are warranted to investigate the clinical significance of this technique.
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Adhesivos/uso terapéutico , Hemorragia/terapia , Hemostasis , Hepatectomía/métodos , Albúmina Sérica , Alanina Transaminasa/sangre , Fosfatasa Alcalina/sangre , Animales , Aspartato Aminotransferasas/sangre , Coagulación Sanguínea/efectos de los fármacos , Humanos , Pruebas de Función Hepática , Ensayo de Materiales , Modelos Animales , PorcinosRESUMEN
Using the delta-P(1) approximation to the Boltzmann transport equation we develop analytic solutions for the fluence rate produced by planar (1-D) and Gaussian beam (2-D) irradiation of a homogeneous, turbid, semi-infinite medium. To assess the performance of these solutions we compare the predictions for the fluence rate and two metrics of the optical penetration depth with Monte Carlo simulations. We provide results under both refractive-index matched and mismatched conditions for optical properties where the ratio of reduced scattering to absorption lies in the range 0< or =(micro(s')/micro(a))< or =10(4). For planar irradiation, the delta-P(1) approximation provides fluence rate profiles accurate to +/-16% for depths up to six transport mean free paths (l*) over the full range of optical properties. Metrics for optical penetration depth are predicted with an accuracy of +/-4%. For Gaussian irradiation using beam radii r(0) > or =3 l*, the accuracy of the fluence rate predictions is no worse than in the planar irradiation case. For smaller beam radii, the predictions degrade significantly. Specifically for media with (micro(s')/micro(a))=1 irradiated with a beam radius of r(0)=l*, the error in the fluence rate approaches 100%. Nevertheless, the accuracy of the optical penetration depth predictions remains excellent for Gaussian beam irradiation, and degrades to only +/-20% for r(0)=l*. These results show that for a given set of optical properties (micro(s')/micro(a)), the optical penetration depth decreases with a reduction in the beam diameter. Graphs are provided to indicate the optical and geometrical conditions under which one must replace the delta-P(1) results for planar irradiation with those for Gaussian beam irradiation to maintain accurate dosimetry predictions.
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Tejido Conectivo/fisiología , Luz , Modelos Biológicos , Modelos Estadísticos , Nefelometría y Turbidimetría/métodos , Radiometría/métodos , Simulación por Computador , Dosis de Radiación , Dispersión de RadiaciónRESUMEN
If a single optical fiber is used for both delivery and collection of light, two major factors affect the measurement of collected light: (1) the light transport in the medium that describes the amount of light that returns to the fiber and (2) the light coupling to the optical fiber that depends on the angular distribution of photons entering the fiber. We focus on the importance of the latter factor and describe how the efficiency of the coupling depends on the optical properties of the medium. For highly scattering tissues, the efficiency is well predicted by the numerical aperture (NA) of the fiber. For lower scattering, such as in soft tissues, photons arrive at the fiber from deeper depths, and the coupling efficiency could increase twofold to threefold above that predicted by the NA.
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Tecnología de Fibra Óptica , Modelos Teóricos , Método de Montecarlo , Fibras Ópticas , Fantasmas de ImagenRESUMEN
BACKGROUND AND OBJECTIVES: We compared ureteral anastomosis using a laser and intraluminal albumin stent with both conventional suturing and laser soldering techniques. STUDY DESIGN/MATERIALS AND METHODS: Twelve pigs underwent bilateral ureteral anastomoses (N = 24) using one of the three anastomotic methods: (1) laser welding with intraluminal albumin stent (N = 11); (2) with albumin solder (N = 8); and (3) conventional suturing (N = 5). Operative parameters, leakage rate, intrapelvic perfusion pressure, urography, and histology of the anastomoses were examined. RESULTS: Operative time for ureteral anastomosis in the stent and solder groups were significantly shorter than the suture group (means 370 seconds and 360 vs. 710 seconds, both P = 0.02). Leakage rate of the anastomoses was lower in the stent group (9%, 1/11) as compared to the solder group (25%, 2/8). The Whitaker test showed that the intrapelvic perfusion pressure elevated gradually after anastomosis and significantly increased at 4 weeks postoperatively in all three methods. Various degrees of hydronephrosis were also noticed in three groups after 4 weeks of surgery. CONCLUSIONS: Use of the intraluminal albumin stent increased the reliability of laser welding for ureteral anastomosis. The clinical significance of using this technique should be investigated further.