RESUMEN
A new diterpenoid compound named glutinic acid had been isolated from Caryopteris glutinosa Rehd by liquid chromatography on Al2O3 and silica gel column. The structure of glutinic acid was elucidated on the basis of IR, UV, MS, H1-NMR, 13C-NMR, DEPT and 13C-1H-COSY spectral analyses.
Asunto(s)
Diterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Conformación MolecularRESUMEN
Following mutagenesis of cultured lepidopteran cells (Spodoptera frugiperda) by ethylmethanesulfonate, three variants resistant to 5-bromodeoxyuridine (BrdUrd) were isolated. These clones were 100- to 200-fold more resistant to BrdUrd than the parental cells and were shown to be deficient in thymidine kinase (TK). The drug-resistant phenotype was stable for up to three years of culture under nonselective conditions. It was also found that the S. frugiperda cell line was highly resistant to aminopterin. A selective medium was formulated and used to select herpes simplex virus thymidine kinase (HSV-TK) transfectants from the TK-deficient cells.
Asunto(s)
Bromodesoxiuridina/farmacología , Línea Celular , Simplexvirus/genética , Timidina Quinasa/genética , Transfección , Animales , Línea Celular/efectos de los fármacos , ADN Recombinante , Resistencia a Medicamentos , Genes Virales , Mariposas Nocturnas/citología , Simplexvirus/enzimologíaRESUMEN
The gene coding for the hepatitis B virus surface antigen (HBsAg) under the control of Autographa californicanuclear polyhedrosis virus polyhedrin promoter was successfully inserted into the genome of the Trichoplusia ni nuclear polyhdrosis virus. Infection of Spodoptera frugiperda cells with this recombinant virus produced a significant amount of HBsAg protein and secreted 22 nm particles containing the HBsAg. The expression of HBsAg gene was also obtained both in Trichoplusia ni larvae and in Philosamia cynthia ricini prepupae when infected with the recombinant virus. The HBsAg proteins expressed by baculovirus vector systems have morphological and antigenic properties identical to the 22 nm particles secreted by human cells.