RESUMEN
AIMS: To investigate the capability, properties, and molecular mechanism of inulin fermentation by lactic acid bacteria (LAB) from Sichuan pickle. METHODS AND RESULTS: A total of 79 LAB strains were purified from 30 aged Sichuan pickle brine samples, and only 21 Lactiplantibacillus plantarum strains (26.58%, 21/79) derived from 15 samples grew well through utilizing inulin as a carbon source. The fermentation tests through using long-chain inulin (lc-inulin) as a carbon source showed that only 6 L. plantarum strains grew well, while other 15 strains could only utilize short-chain oligofructose (FOS), and thin-layer chromatography analysis evidenced a strain specificity of inulin consumption patterns. Lactiplantibacillus plantarum YT041 is a vigorous inulin fermenter, and whole genome sequencing data revealed that sacPTS1 and fosRABCDXE operons might be associated with the fermentation of FOS and lc-inulin, respectively. CONCLUSIONS: The phenotype of inulin consumption is commonly present in LAB from Sichuan pickle, which is strain-specific and largely depends on their specific ecological niche and degree of polymerization.
Asunto(s)
Alimentos Fermentados , Lactobacillales , Lactobacillus plantarum , Inulina/metabolismo , Lactobacillales/metabolismo , Genómica , Fenotipo , Alimentos Fermentados/microbiología , Fermentación , Lactobacillus plantarum/genética , Lactobacillus plantarum/metabolismoRESUMEN
Two polysaccharides from Carthamus tinctorius L. (CTLP-1 and CTLP-2) were purified, and their structures were analyzed by physical and chemical testing. CTLP-1 had a mass of 5900 Da that was composed of arabinose, glucose, and galactose with a mass molar ratio of 6.7:4.2:1. The backbone of CTLP-1 was â1)-α-GalAp-(1â4)-α-Arap-(1â2)-α-Glup-(4â. CTLP-2 had a mass of 8200 Da that was composed of arabinose, glucose, and galactose with a mass molar ratio of 16.76:4.28:1. The backbone of CTLP-2 was â1)-α-Galp-(2,6 â1)-α-Arap-(4,6 â1)-α-Glup-(3â. Both of them exhibited a high reducing power, hydroxyl radical scavenging activity, DPPH radical scavenging activity and ABTS radical scavenging activity, moderate Fe2+ chelating activity and superoxide anion scavenging activity, implying that they might be potential antioxidants.
RESUMEN
A novel carbamate- and cyano-containing alkaloid, aspeverin (1), was isolated from the culture of an algicolous Aspergillus versicolor strain (dl-29). The structure and absolute configuration were unambiguously identified by NMR, IR, ECD, and mass spectrometric methods as well as quantum chemical calculations. 1 exhibited potent bioactivities against some marine-derived organisms.
Asunto(s)
Alcaloides/síntesis química , Aspergillus/química , Carbamatos/síntesis química , Alcaloides/química , Carbamatos/química , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Estructura MolecularRESUMEN
A new oxylipin, (8E,12Z)-10,11-dihydroxyoctadeca-8,12-dienoic acid (1), a new steroid, 3ß,4α-dihydroxy-26-methoxyergosta-7,24(28)-dien-6-one (2), and four known steroids, episterol (3), (22E,24R)-ergosta-7,22-dien-3ß,5α,6α-triol (4), (22E,24R)-ergosta-5,22-dien-3ß-ol (5), and (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one (6), were isolated from the cultures of Aspergillus flavus, an endophytic fungus isolated from the marine red alga Corallina officinalis. Their structures and relative stereochemistry were elucidated by 1D, 2D NMR and mass spectroscopic techniques. 1 and 2 exhibited low activity to inhibit acetylcholinesterase and no activity against plant pathogenic fungi Colletotrichum lagenarium and Fusarium oxysporum.
Asunto(s)
Aspergillus flavus/química , Inhibidores de la Colinesterasa/aislamiento & purificación , Oxilipinas/aislamiento & purificación , Esteroides/aislamiento & purificación , Acetilcolinesterasa/metabolismo , Inhibidores de la Colinesterasa/química , Inhibidores de la Colinesterasa/farmacología , Espectroscopía de Resonancia Magnética/normas , Estructura Molecular , Oxilipinas/química , Oxilipinas/farmacología , Estándares de Referencia , Estereoisomerismo , Esteroides/química , Esteroides/farmacología , Relación Estructura-ActividadRESUMEN
Asporyergosterol (1), a new steroid with an E double bond between C-17 and C-20, was identified from the culture extracts of Aspergillus oryzae, an endophytic fungus isolated from the marine red alga Heterosiphonia japonica. Moreover, four known steroids including (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one (2), (22E,24R)-3beta-hydroxyergosta-5,8,22-trien-7-one (3), (22E,24R)-ergosta-7,22-dien-3beta,5alpha,6beta-triol (4), and (22E,24R)-5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol (5) were isolated. Structures of these compounds were unambiguously established by spectroscopic techniques and by comparison with literature values. All the isolates exhibited low activity to modulate acetylcholinesterase (AChE).
Asunto(s)
Aspergillus oryzae/química , Colinérgicos/aislamiento & purificación , Ergosterol/análogos & derivados , Rhodophyta/microbiología , Esteroides/aislamiento & purificación , Aspergillus oryzae/aislamiento & purificación , Ergosterol/química , Ergosterol/aislamiento & purificación , Estructura Molecular , Esteroides/químicaRESUMEN
Two new indoloditerpene derivatives asporyzin A (1) and asporyzin B (2), one new indoloditerpene asporyzin C (3), and three known related indoloditerpenes JBIR-03 (4), emindole SB (5), and emeniveol (6) were isolated from an endophytic fungus Aspergillus oryzae, isolated from the marine red alga Heterosiphonia japonica. Their structures were unambiguously established by spectroscopic techniques. In addition, all the isolates were evaluated preliminarily for insecticidal and antimicrobial activities in order to probe into their chemical defensive function. Compound 4 was more active against brine shrimp than the others, and 3 possessed potent activity against Escherichia coli.