RESUMEN
Meibomian gland dysfunction (MGD) is an epidemic chronic ocular inflammation. However, little is known about its effective treatment. Here, this study identified important MGD-related genes, core regulators, and potential drugs and their targets though integrating a series of bioinformational analyses. First, there were 665 differentially expression genes (DEGs) were identified. Then, 56 coexpression modules were exacted based on the expression of DEGs and their interactors. Moreover, core transcription factors (TF) significantly regulated modules were identified, including RELA, HIF1A, SIRT1, and MYC, which related to variety of eye diseases. Finally, the prediction of potential drugs and the identification of their target were performed. The results showed that artenimol, copper, and glutathione may have the remarkable curative effect or the toxicology to MGD. Moreover, their targets module gene LDHA (lactate dehydrogenase A), ENO1 (enolase 1), ALB (albumin), and PKM (pyruvate kinase M) are play important role in eye diseases. It suggests that these potential drugs may be useful for the treatment of MGD by acting on their targets. It provides valuable references for drug redirection and new drug development for drug developers, and provides individualized treatment strategies for tarsal gland dysfunction.
Asunto(s)
Artemisininas/farmacología , Biomarcadores/análisis , Regulación de la Expresión Génica/efectos de los fármacos , Disfunción de la Glándula de Meibomio/patología , Medicina de Precisión , Biomarcadores/metabolismo , Biomarcadores de Tumor/genética , Cobre/farmacología , Proteínas de Unión al ADN/genética , Perfilación de la Expresión Génica , Glutatión/farmacología , Humanos , L-Lactato Deshidrogenasa/genética , Disfunción de la Glándula de Meibomio/clasificación , Disfunción de la Glándula de Meibomio/tratamiento farmacológico , Disfunción de la Glándula de Meibomio/genética , Fosfopiruvato Hidratasa/genética , Piruvato Quinasa/genética , Albúmina Sérica Humana/genética , Proteínas Supresoras de Tumor/genéticaRESUMEN
AIM OF THE STUDY: This study aimed to observe the expressions of heat shock protein 27 (HSP27) and proliferating cell nuclear antigen (PCNA) in retinoblastoma (Rb) cells and to explore the relationships of the expression with Rb differentiation and optic nerve infiltration. MATERIAL AND METHODS: Heat shock protein 27 and PCNA expressions in 36 routine Rb paraffin specimens were observed using PV9000 two-stage immunohistochemical staining. The correlations of the HSP27 and PCNA expressions with Rb differentiation and optic nerve infiltration were analyzed. RESULTS: Heat shock protein 27 was weakly expressed in the normal retina, specifically in the ganglion cell layer. It was extensively expressed in Rb tissues at a positive rate of 69.4%, and the positive substances were primarily located in the cytoplasm. Proliferating cell nuclear antigen was expressed weakly or not at all expressed in the normal retina and was extensively expressed in Rb tissues at a positive rate of 83.3%, and the positive substances were primarily located in the nucleus. The positive expression rates of HSP27 and PCNA in the differentiated group were significantly higher than in the undifferentiated group (p < 0.05). The positive expression rates of HSP27 and PCNA in the optic nerve-infiltrated group were significantly higher than in the non-infiltrated group (p < 0.05). Heat shock protein 27 expression was positively correlated with PCNA expression in Rb (p < 0.01). CONCLUSIONS: Heat shock protein 27 and PCNA expressions are markedly correlated with cell differentiation and optic nerve infiltration in Rb.
