Effect of supplementation of roasted oilseeds and phytochemical-rich herbages on nutrient utilization, methane emission, and growth performance in finisher lambs.

Ruminal methanogenesis is a major contributor to global environmental pollution in the agriculture sector. Dietary intervention modestly abates enteric methane emissions in ruminants. Therefore, the present experiment was conducted to evaluate the combined effect of dietary oilseeds and phytochemical-rich herbages on enteric methane emission, growth performance, and nutrient utilization in lambs. Forty-eight finisher Malpura lambs were divided into 4 groups (RSZ, RSP, RSLZ, and RSLP) of 12 each in a factorial design. Lambs were fed ad libitum concentrate formulated with roasted soybean (RS) or roasted soybean plus linseed (RSL) along with Ziziphus nummularia (Z) or Prosopis cineraria (P) leaves as roughage sources. Variation in the source of roughage significantly affected feed intake, and it was higher (P < 0.05) in lambs fed Prosopis cineraria leaves (RSP and RSLP). The average daily gain was improved (P < 0.05) by 28.6 and 25.0% in lambs fed Prosopis cineraria, i.e., RSP and RSLP respectively, than those fed Ziziphus nummularia leaves irrespective of concentrate diets. Overall, microbial nitrogen synthesis (MNS) was higher (P < 0.05) in lambs fed roasted soybean (RS) than roasted soybean plus linseed (RSL); however, combination of Prosopis cineraria with both the concentrate diets increased MNS, than the combination of roasted oilseeds and Ziziphus nummularia. No significant interaction was observed in the concentration and proportion of volatile fatty acids by feeding combination of roasted oilseed and tree leaves; however, the proportion of acetic and propionic acid was higher (P < 0.05) in the RSL group compared to RS. Methane emission per kg dry matter intake was reduced (P < 0.05) by feeding Prosopis cineraria leaves regardless of concentrate mixture. As a result, the loss of metabolizable energy through methane emission was lowered by 0.7 and 4.6% when Prosopis cineraria leaves were combined with roasted soybean (RSP) and roasted soybean plus linseed (RSLP), respectively. From the present findings, it can be concluded that combination of Prosopis cineraria leaves either with roasted soybean or roasted soybean plus linseed reduced enteric methane emissions more effectively than Ziziphus nummularia leaves, and the energy saved was transformed into higher body weight gain and improved feed conversion ratio.

Dietary n-3 PUFA augments pre-ovulatory follicle turnover and prolificacy in well-fed ewes.

The present study evaluated the effect of dietary supplementation with n-3 polyunsaturated fatty acids (PUFA) on preovulatory follicle (POF) turnover, prolificacy, and endocrine and metabolic milieu in Malpura sheep. Fifty cyclic ewes with 3-3.5 body condition scores on a five-point scale were allocated equally to two groups (n = 25) following estrus synchronization and were supplemented with 0.6 mL/kg body weight of n-3 PUFA-rich fish oil (FO) or palm oil (PO) as control, for 60 d following an acclimatization period of 7 d. All ewes were mated with sexually active rams at the end of the supplementation period. On ultrasonographic ovarian scanning at the last fourth estrus, the mean number of POFs was 77.8% greater (P < 0.01) in FO ewes than in the PO ewes. The proportion of ewes with multiple ovulations two months after the beginning of supplementation was 56% in the FO group as compared to 8% in the PO group. The number of fetuses was 46% higher (P < 0.01) in the FO than in the PO ewes at d 45 of gestation. At lambing, the twinning percent in the FO ewes was three times greater than in the PO ewes (27.3 vs. 9.1%). Plasma cholesterol, estradiol, and insulin concentrations were lower (P < 0.01) in ewes fed with FO than those offered PO group at the end of the feeding period. It was concluded that the dietary supplementation of n-3 PUFA-rich FO in well-fed Malpura ewes improved the number of follicles and ovulation rate which led to an increased prolificacy, accompanied by a reduction of plasma cholesterols, estradiol, and insulin.

Chemical Constituents Based Approach for the Management of Diabetes.

BACKGROUND: A number of complexities in compliance with long-term diabetes have been elicited. It has become a global concern without any convincing medicinal, therapeutical methodology. Both hyperglycaemia and oxidative pressure are major notable parts that play a significant role in the initialization of diabetic inconvenience. Natural medications have gained a lot of attention in recent years as expected restorative specialists in the prevention and treatment of diabetic complications due to their many objectives and less poisonous outcomes. This survey means to evaluate the accessible information on therapeutic spices for constriction and the executives of diabetic complications. MATERIALS AND METHODS: Bibliographic investigation was accomplished by checking old-style course books and papers, directing overall bases of logical information (SCOPUS, PUBMED, SCIELO, Google Scholar, NISCAIR,) to recapture accessible distributed writing. For the assessment of plants with the potential in calming diabetic complications, several inclusion models rely on the numerous medicinal spices as well as their crucial mixes. Furthermore, several models, including plants, have been considered, each of which has a suitable impact on increasing oxidative pressure in diabetes. RESULTS: Different therapeutic plants/plant withdrawals containing alkaloids, terpenoids, phenolic compounds, flavonoids, saponins, and phytosterol-type synthetic constituents were uncovered that are profitable in the administration of diabetic complexities. Results may be attributed to the improvement of oxidative pressure, constant hyperglycemia, and twitch of different metabolic pathways related to the pathogenesis of diabetic confusions. CONCLUSION: An optimistic approach for new medication terminology to treat diabetic confusion is screening compound competitors from homegrown medication. Investigation of the activity of different plant extracts as well as their potency profile and to determine their job in the treatment of diabetic inconveniences must be there. In addition, an ideal rat model which imitates human diabetic complications ought to be created.

High resolution cryo-EM structures of two potently SARS-CoV-2 neutralizing monoclonal antibodies of same donor origin that vary in neutralizing Omicron variants

While vaccines have by large been found to effective against the evolving SARS-CoV-2 variants, the profound and rapid effectivity of monoclonal antibodies (mAbs) in significantly reducing hospitalization to severe disease outcomes have also been demonstrated. In the present study, by high resolution cryo-electron microscopy (cryo-EM), we examined the structural insights of two trimeric spike (S) protein bound mAbs isolated from an Indian convalescent individual infected with ancestral SARS-CoV-2 which we recently reported to potently neutralize SARS-CoV-2 from its ancestral form through highly virulent Delta form however different in their ability to neutralize Omicron variants. Our findings showed binding and conformational heterogeneities of both the mAbs (THSC20.HVTR04 and THSC20.HVTR26) bound to S trimer in its apo and hACE-2 bound forms. Additionally, cryo-EM resolved structure assisted modeling highlighted key residues associated with the ability of these two mAbs to neutralize Omicron variants. Our findings highlighted key interacting features modulating antigen-antibody interacting that can further aid in structure guided antibody engineering to enhance their breadth and potency. HighlightsO_LITwo potent human mAbs obtained from a single donor differ binding to Omicron spikes C_LIO_LIPattern of binding and conformation of these mAbs bound to full length spike differs C_LIO_LIAntibody binding alters the conformational states of S trimer in its apo and hACE-2 bound forms. C_LIO_LICryo-EM structure guided modeling highlighted correlates of interacting residues associated with resistance and sensitivity of BA.1, BA.2, BA.4/BA.5 resistance and sensitivity against these mAbs. C_LI

α-Lipoic Acid, an Organosulfur Biomolecule a Novel Therapeutic Agent for Neurodegenerative Disorders: An Mechanistic Perspective.

Lipoic acid (α-LA) (1,2-dithiolane3-pentanoic acid (C8H14O2S2) is also called thioctic acid with an oxidized (disulfide, LA) and a reduced (di-thiol: dihydro-lipoic acid, DHLA) form of LA. α-LA is a potent anti-oxidative agent that has a significant potential to treat neurodegenerative disorders. α-LA is both hydrophilic and hydrophobic in nature. It is widely distributed in plants and animals in cellular membranes and in the cytosol, which is responsible for LA's action in both the cytosol and plasma membrane. A systematic literature review of Bentham, Scopus, PubMed, Medline, and EMBASE (Elsevier) databases was carried out to understand the Nature and mechanistic interventions of the α-Lipoic acid for central nervous system diseases. Moreover, α-LA readily crosses the blood-brain barrier, which is a significant factor for CNS activities. The mechanisms of α-LA reduction are highly tissue-specific. α-LA produces its neuroprotective effect by inhibiting reactive oxygen species formation and neuronal damage, modulating protein levels, and promoting neurotransmitters and anti-oxidant levels. Hence, the execution of α-LA as a therapeutic ingredient in the therapy of neurodegenerative disorders is promising. Finally, based on evidence, it can be concluded that α-LA can prevent diseases related to the nervous system.

Highly Thermotolerant SARS-CoV-2 Vaccine Elicits Neutralising Antibodies Against Delta and Omicron in Mice

As existing vaccines fail to completely prevent COVID-19 infections or community transmission, there is an unmet need for vaccines that can better combat SARS-CoV-2 variants of concern (VOC). We have previously developed highly thermo-tolerant monomeric and trimeric receptor binding domain derivatives that can withstand 100{degrees}C for 90 minutes and 37{degrees}C for four weeks, and help eliminate cold chain requirements. We show that mice immunised with these vaccine formulations elicit high titres of antibodies that neutralise SARS-CoV-2 variants VIC31 (with Spike: D614G mutation), Delta and Omicron (BA.1.1) VOC. Compared to VIC31, there was an average 14.4-fold reduction in neutralisation against BA.1.1 for the three monomeric antigen-adjuvant combinations, and 16.5-fold reduction for the three trimeric antigen-adjuvant combinations; the corresponding values against Delta were 2.5 and 3.0. Our findings suggest that monomeric formulations are suitable for the upcoming Phase I human clinical trials, and that there is potential for increasing efficacy with vaccine matching to improve responses against emerging variants. These findings are consistent with in silico modelling and AlphaFold predictions which show that while oligomeric presentation can be generally beneficial, it can make important epitopes inaccessible, and also carries the risk of eliciting unwanted antibodies against the oligomerisation domain.

Growth performance, rumen fermentation and economic analysis of Malpura lambs raised on milk replacer at different weaning age under semiarid conditions.

Pre-weaning nutrition has significant influence on post-weaning performance of lambs. Strategic weaning management on milk replacer allowance at this phase can be a promising approach for harvesting desired carcass trait in finisher phase. Therefore, present experiment aimed economizing pre-weaning weight gain in lambs by feeding milk replacer and subsequently minimizing weaning age. A study of 90 days duration was conducted to determine the production performance, nutrient utilization and rumen fermentation of lambs on milk replacer allowance at different weaning age. Sixty-six Malpura lambs (10 days of age) were randomly divided into 3 equal groups. Lambs in control group were allowed conventional free suckling of dam up to 90 day of age, whereas lambs in the two treatment groups, that is W60 and W90 were offered milk replacer and weaned at 60 and 90 days, respectively. Lambs in all groups were offered ad-libitum creep mixture, Ziziphus nummularia leaves, Vigna unguiculata hay and fresh Ailanthus excelsa leaves. Overall weight gain was 12.7 per cent higher (p < 0.05) in W90 than control whereas in W60 group it was statistically comparable with both control and W90 groups. Overall average daily gain was 12.1 per cent higher (p < 0.05) in W90 group as compared to control. In case of nutrient digestibility, there was significant depression of dry matter (DM), organic matter (OM), crude protein (CP) and neutral detergent fibre (NDF) by 16.9, 17.2, 9.3 and 32.0 per cent in W60 than control; however, in W90, CP digestibility was higher (p < 0.05) than control and W60. Considering economic analysis, the net return obtained from W90 (90 days weaning plus milk replacer allowance) was more 20.0 per cent higher than control. Therefore, it can be concluded that lamb rearing on milk replacer and weaning at 90 days have a sustained positive effect on lambs' production performance and is more profitable than the practice of milk replacer allowance and early weaning in semiarid conditions.

A highly thermotolerant, trimeric SARS-CoV-2 receptor binding domain derivative elicits high titers of neutralizing antibodies

The Receptor Binding Domain (RBD) of SARS-CoV-2 is the primary target of neutralizing antibodies. We designed a trimeric, highly thermotolerant glycan engineered RBD by fusion to a heterologous, poorly immunogenic disulfide linked trimerization domain derived from cartilage matrix protein. The protein expressed at a yield of [~]80-100 mg/liter in transiently transfected Expi293 cells, as well as CHO and HEK293 stable cell lines and formed homogeneous disulfide-linked trimers. When lyophilized, these possessed remarkable functional stability to transient thermal stress of upto 100 {degrees}C and were stable to long term storage of over 4 weeks at 37 {degrees}C unlike an alternative RBD-trimer with a different trimerization domain. Two intramuscular immunizations with a human-compatible SWE adjuvanted formulation, elicited antibodies with pseudoviral neutralizing titers in guinea pigs and mice that were 25-250 fold higher than corresponding values in human convalescent sera. Against the beta (B.1.351) variant of concern (VOC), pseudoviral neutralization titers for RBD trimer were [~] three-fold lower than against wildtype B.1 virus. RBD was also displayed on a designed ferritin-like Msdps2 nanoparticle. This showed decreased yield and immunogenicity relative to trimeric RBD. Replicative virus neutralization assays using mouse sera demonstrated that antibodies induced by the trimers neutralized all four VOC to date, namely B.1.1.7, B.1.351, P.1 and B.1.617.2 without significant differences. Trimeric RBD immunized hamsters were protected from viral challenge. The excellent immunogenicity, thermotolerance, and high yield of these immunogens suggest that they are a promising modality to combat COVID-19, including all SARS-CoV-2 VOC to date.

Evaluation of Gamma Amino Butyric Acid (GABA) and Glibenclamide Combination Therapy in Streptozotocin Induced Diabetes.

BACKGROUND: Type 1-diabetes (T1D) is characterized by autoimmune destruction of ß-cells and loss of endogenous insulin. A lifelong dependency on exogenous supply of insulin presents a great challenge in the pharmacotherapy of T1D that elicits a quest for alternative therapies, which can protect ß-cells and revive their insulinogenic functions. GABA (γ-aminobutyric acid) has immunoprotective and ß-cell regenerative capabilities. Co-administration of an insulin secretagogue, such as glibenclamide (Glib), along with GABA may enhance the pancreatic insulin output in T1D. OBJECTIVE: The present study evaluated the possible mechanism of GABA in the improvement of glucose tolerance and its effects in streptozotocin (STZ) induced T1D along with Glib. METHODS: Wistar rats (180-220 g) were administered a single dose of STZ (55 mg/kg, i.p.). GABA (100 mg/kg, i.p.) and Glib (5 or 10 mg/kg, i.p.) alone or in combination were administered for 28 days. Body weight (b.w.), water consumption, fasting blood glucose (FBG), oral glucose tolerance, plasma lipids, insulin, and muscle GLUT-4 (glucose transporters) protein level were assessed. RESULTS: T1D significantly decreased b.w. and increased water-intake in rats. An increase in FBG and a decrease in plasma insulin and muscle GLUT-4 indicated STZ-triggered destruction of ß-cells in diabetic rats accompanied with dyslipidemia. GABA or Glib (10 mg/kg) significantly improved b.w., plasma insulin and GLUT-4 levels, and ameliorated FBG and blood lipid profile in diabetic rats. GABA and Glib (5 mg/kg) combination therapy achieved far better control over hyperglycemia and related pathogenic conditions (b.w., water-intake, insulin, GLUT-4, lipids). The anti-diabetic effect of combination therapy was significantly more pronounced in comparison to individual drug treatments. Histopathological analysis revealed an increase in the number of functional pancreatic-islets by combination therapy. CONCLUSION: GABA revitalized ß-cells against STZ-toxicity. GABA and Glib synergistically augmented insulin secretion that can be used to manage T1D and its complications. GABA has the potential to remarkably enhance the therapeutic outcome in diabetic patients and reduce the dose of existing anti-diabetic drugs such as Glib.

Design of a highly thermotolerant, immunogenic SARS-CoV-2 spike fragment

Virtually all SARS-CoV-2 vaccines currently in clinical testing are stored in a refrigerated or frozen state prior to use. This is a major impediment to deployment in resource-poor settings. Several use viral vectors or mRNA. In contrast to protein subunit vaccines, there is limited manufacturing expertise for these novel, nucleic acid based modalities, especially in the developing world. Neutralizing antibodies, the clearest known correlate of protection against SARS-CoV-2, are primarily directed against the Receptor Binding Domain (RBD) of the viral spike protein. We describe a monomeric, glycan engineered RBD protein fragment that is expressed at a purified yield of 214mg/L in unoptimized, mammalian cell culture and in contrast to a stabilized spike ectodomain, is tolerant of exposure to temperatures as high as 100{degrees}C when lyophilized, upto 70{degrees}C in solution and stable for over four weeks at 37{degrees}C. In prime:boost guinea pig immunizations, when formulated with the MF59 like adjuvant AddaVax, the RBD derivative elicited neutralizing antibodies with an endpoint geometric mean titer of ~415 against replicative virus, comparing favourably with several vaccine formulations currently in the clinic. These features of high yield, extreme thermotolerance and satisfactory immunogenicity suggest that such RBD subunit vaccine formulations hold great promise to combat COVID-19.

Effect of complete feed block with tree leaves rich in hydrolysable and condensed tannins on nutrient utilization, rumen fermentation and growth performance of lambs.

Thirty lambs (Avishaan genotype, 10 weeks old, 14.0 ± 0.2 kg live weight) were randomly assigned into three equal treatment groups to study the effect of complete feed blocks (CFB; concentrate and roughage at 70:30 ratio) with three different tanniniferous tree leaves on intake and utilization of nutrients, rumen fermentation, microbial protein synthesis and growth performance. The treatments were T1 (Control), CFB1 with Vigna sinensis hay; T2, CFB2 with Acacia nilotica leaves rich in hydrolysable tannins (HT); and T3, CFB3 with Ziziphus nummularia leaves rich in condensed tannins (CT). The three CFBs were fed ad libitum to the respective groups of lambs for a period of 12 weeks. There was lower (p < .05) intake of dry matter (DM), total carbohydrates (TCHO) and fibre components in T2 compared with T1 and T3. However, the digestibility of nutrients except crude protein (CP) was higher in T2. Diet had no effect (p > .05) on the LW gain in lambs. Amongst the three groups, T3 showed enhanced N utilization with a comparable microbial protein synthesis, the lowest being in T2. The T2 group of lambs had higher propionate and lower non-glucogenic: glucogenic short-chain fatty acids ratio. It may be concluded that tanniniferous tree leaves at 30% of total mixed ration can meet the requirement of nutrients for desired post-weaning growth.

Comparison of a waxy maize and a potato starch-based balanced hydroxyethyl starch for priming in patients undergoing coronary artery bypass grafting.

OBJECTIVE: Concerns have been raised about differences in the safety profile of potato- versus waxy maize-derived hydroxyethyl starch (HES). The objective of this study was to compare 2 HES solutions derived from 2 different source materials (potato versus waxy maize) for their dose-related effects on hemostasis and organ function when used to prime the cardiopulmonary bypass circuit (CPB). DESIGN: A prospective, randomized, controlled study. SETTING: Tertiary care center. PARTICIPANTS: Eighty patients undergoing coronary artery bypass grafting (CABG) on CPB. INTERVENTIONS: For priming the CPB circuit, the HESPRL group received 1000 mL of potato-derived balanced 6% HES 130/0.42 along with 500 mL of Ringer's lactate; the HESP group received 1,500 mL of potato-derived balanced 6% HES 130/0.42; the HESMRL group received 1000 mL of waxy maize-derived balanced 6% HES 130/0.4 along with 500 mL of Ringer's lactate, and the HESM group received 1500 mL of waxy maize-derived balanced 6% HES 130/0.4. MEASUREMENTS AND MAIN RESULTS: There were no significant differences in 24-hour mediastinal drainage, rate of re-exploration, blood product usage, coagulation parameters, and measures of pulmonary, renal, and hepatic function with respect to plant source of HES, when equivalent doses were used. Sonoclot activated clotting time (SonACT) was significantly higher and clot rate (CR) significantly lower at end of surgery (T1) and 24 hours after surgery (T2) in the HESP and HESM groups compared with the HESPRL and HESMRL groups. Compared with baseline, CR and platelet function were significantly lower at T1, PaO2/FIO2 ratio decreased significantly at T1 and T2, and serum bilirubin and transaminases increased significantly at T2 in all 4 groups. CONCLUSIONS: There was no significant difference in cumulative 24-hour mediastinal drainage when potato-derived balanced 6% HES 130/0.42 or waxy maize-derived balanced 6% HES 130/0.4 was used to prime the CPB circuit in patients undergoing CABG. In equal doses, both starches exerted the same effect on blood coagulation and pulmonary, renal, and hepatic function.

Level and period of realimentation to assess improvement in body condition and carcass quality in cull ewes.

Improvement in body condition was assessed in 40 cull ewes (>6 years), equally distributed in two groups and realimented with ad libitum roughage (gram straw) and two levels of concentrate feeding, i.e., 2.5 % (T(1)) of live weight (LW) and ad libitum (T(2)). Five representative animals from an initial 45 were slaughtered at the initiation of the study (0 day) and five animals from each treatment at 44, 67, and 90 days of experiment for carcass attributes. Improvement in body condition score (BCS), nutrient utilization, feed efficiency, and carcass traits were assessed at 44, 67, and 90 days. Metabolism trial of 6-day collection of feed, feces, and urine samples was conducted on five representative ewes from each group after 60 days of feeding. The level of concentrate feeding on LW gain and BCS was significant, and the duration of realimentation showed a linear improvement (P < 0.001). The digestibility and intake of dry matter, organic matter, and crude protein was higher (P < 0.05) in T(2). The N intake, absorption, and balance showed a similar trend. Increase (P < 0.05) in total N, trichloroacetic acid precipitable N, and ammonia N was observed with extension of realimentation period. Blood metabolic profile also showed improvement (P < 0.05) from an undernourished state to normal after alimentation. Animals in T(2) accumulated higher LW with minimal expenditure of metabolizable energy (73.4 vs 79.1 MJ) and higher efficiency of feed conversion during 68 to 90 days of realimentation. Linear improvement (P < 0.01) in carcass traits (preslaughter weight, empty live weight, hot carcass weight, dressing percentage, and amount of subcutaneous and intramuscular fat) and composition of longissimus dorsi muscle was observed. Ad lib concentrate supplementation for a period of 90 days may thus be considered appropriate for achieving desired efficiency of gain and improvement in body condition of cull ewes for quality mutton production.

Effect of yeast supplementation on the growth performance of Malpura lambs.

Thirty-six Malpura lambs (age = 58 days; 8.9 kg BW) were equally divided into three groups (N = 12; six males and six females) to assess the effect of probiotics supplementation on growth, digestibility, rumen fermentation and carcass attributes. The lambs of the control group (CON) were not supplemented with probiotics, while test groups received either Saccharomyces cerevisiae (SC) or combination of S. cerevisiae and Lactobacillus sporogenes (SCLS) at 1.5 % of concentrate mixture. The lambs were fed ad libitum concentrate mixture and bajra (Pennisetum typhoides) straw in a cafeteria system until 180 days of age. Daily feed intake and weekly live weight changes were recorded. A metabolism trial was conducted on six lambs at 90 days. Rumen fermentation study was conducted at 105 days. At 6 months, all male lambs were slaughtered and carcass characteristics were recorded. Body weight and average daily gain (ADG) were similar among the three groups. The digestibilities of all the nutrients were also similar among the groups, except acid detergent fiber (ADF) digestibility, which was higher (P = 0.032) in SC and SCLS than the CON. The rumen fluid pH was higher (P = 0.04) in CON and SC group than SCLS at 0 h while NH(3)-N at 8 h sample was higher (P = 0.031) in SC and SCLS group than the control. Pre-slaughter weight, hot carcass weight and dressing yields were similar. ADF digestibility and rumen fermentation was improved in lambs by probiotic supplementation. However, carcass traits remained unchanged due to probiotics supplementation.

Bupropion as an augmenting agent in patients of depression with partial response.

The objective of this study is to evaluate the effects of bupropion as an add-on therapy to selective serotonin reuptake inhibitor (SSRI) on patients of major depressive disorder with partial response. This prospective, randomized, controlled and single-blind study was conducted in sixty patients suffering from major depressive disorder as per Diagnostic and Statistical Manual (DSM)-IV TR criteria, who were having Hamilton depression rating scale (HDRS) score ≥16 after 4 weeks of treatment with SSRIs. Group A received SSRI plus placebo and group B received SSRI plus bupropion. Evaluation was performed based on changes in HDRS score, Montgomery and Asberg depression rating scale (MADRS), Amritsar depressive inventory (ADI) and spontaneously reported adverse effects. There was a significant decrease in the HDRS, MADRS and ADI scores as compared to baseline in both groups. However, the mean decrease in depression score was more in group B than in group A. The percentage decrease of remitters was also significantly more in group B (60% as per HDRS score and 63% as per MADRS score), as compared to group A (24% as per HDRS score and 27% as per MADRS score) (p < 0.05), at the end of treatment. In conclusion, bupropion add-on can act as augmenting agent in patients of depression with partial response to SSRIs.

Biceps tendinitis as a cause of acute painful knee after total knee arthroplasty.

The case report highlights an unusual case of posterolateral knee pain after total knee arthroplasty. Tendinitis of the patellar tendon or pes anserinus is a common complication after total knee arthroplasty; however, there is no report in the literature regarding the biceps femoris tendinitis causing acute pain in the early postoperative period. In this case, the biceps tendinitis was diagnosed and treated by ultrasound-guided injection into the tendon sheath.