RESUMEN
BACKGROUND: Previously, we observed that hypothermia, widely used for organ preservation, elicits mitochondrial fission in different cell types. However, temperature dependence, mechanisms and consequences of this cold-induced mitochondrial fission are unknown. Therefore, we here study cold-induced mitochondrial fission in endothelial cells, a cell type generally displaying a high sensitivity to cold-induced injury. METHODS: Porcine aortic endothelial cells were incubated at 4-25 °C in modified Krebs-Henseleit buffer (plus glucose to provide substrate and deferoxamine to prevent iron-dependent hypothermic injury). RESULTS: Cold-induced mitochondrial fission occurred as early as after 3 h at 4 °C and at temperatures below 21 °C, and was more marked after longer cold incubation periods. It was accompanied by the formation of unusual mitochondrial morphologies such as donuts, blobs, and lassos. Under all conditions, re-fusion was observed after rewarming. Cellular ATP content dropped to 33% after 48 h incubation at 4 °C, recovering after rewarming. Drp1 protein levels showed no significant change during cold incubation, but increased phosphorylation at both phosphorylation sites, activating S616 and inactivating S637. Drp1 receptor protein levels were unchanged. Instead of increased mitochondrial accumulation of Drp1 decreased mitochondrial localization was observed during hypothermia. Moreover, the well-known Drp1 inhibitor Mdivi-1 showed only partial protection against cold-induced mitochondrial fission. The inner membrane fusion-mediating protein Opa1 showed a late shift from the long to the fusion-incompetent short isoform during prolonged cold incubation. Oma1 cleavage was not observed. CONCLUSIONS: Cold-induced mitochondrial fission appears to occur over almost the whole temperature range relevant for organ preservation. Unusual morphologies appear to be related to fission/auto-fusion. Fission appears to be associated with lower mitochondrial function/ATP decline, mechanistically unusual, and after cold incubation in physiological solutions reversible at 37 °C.
Asunto(s)
Aorta/metabolismo , Frío , Células Endoteliales/metabolismo , Mitocondrias/metabolismo , Dinámicas Mitocondriales , Adenosina Trifosfato/metabolismo , Animales , Biomarcadores , Supervivencia Celular , Células Cultivadas , Dinaminas/genética , Dinaminas/metabolismo , GTP Fosfohidrolasas/metabolismo , Potencial de la Membrana Mitocondrial , Mitocondrias/genética , Mitocondrias/ultraestructura , Fosforilación , Porcinos , Factores de TiempoRESUMEN
Mitochondrial integrity and function constitute a prerequisite for cellular function and repair processes. We have previously shown that mitochondria of different cell types exhibit pronounced fragmentation under hypothermic conditions. This fission, accompanied by a decline of cellular ATP content, showed reversibility at 37â¦C. However, it is unclear whether other temperatures as currently discussed for reconditioning of organs allow this reconstitution of mitochondria. Therefore, we here study in a model of cultured porcine aortic endothelial cells how different rewarming temperatures affect mitochondrial re-fusion and function. After 48 h cold incubation of endothelial cells in Krebs-Henseleit buffer with glucose (5 mM) and deferoxamine (1 mM) at 4â¦C pronounced mitochondrial fission was observed. Following 2 h rewarming in cell culture medium, marked fission was still present after rewarming at 10⦠or 15â¦C. At 21â¦C some re-fusion was visible, which became more marked at 25â¦C. Networks of tubular mitochondria similar to control cells only re-appeared at 37â¦C. ATP content decreased at 4â¦C from 3.6 ± 0.4 to 1.6 ± 0.4 nmol/106 cells and decreased even further when rewarming cells to 10⦠and 15â¦C. Values after rewarming at 21â¦C were similar to the values before rewarming while ATP gradually increased at higher rewarming temperatures. Metabolic activity dropped to 5 ± 11% of control values during 4â¦C incubation and recovered with increasing temperatures to 36 ± 10% at 25â¦C and 78 ± 17% at 37â¦C. Integrity of monolayers, largely disturbed at 4â¦C (large gaps between endothelial cells; cell injury ≤ 1%), showed partial recovery from 15â¦C upwards, complete recovery at 37â¦C. Endothelial repair processes (scratch assay) at 25â¦C were clearly inferior to those at 37â¦C. These data suggest that reconditioning temperatures below 21â¦C are not optimal with regard to reconstitution of mitochondrial integrity and function. For this goal, temperatures of at least 25â¦C appear required, with 30â¦C being superior and 37â¦C yielding the best results.