RESUMEN
To evaluate the potential of cooked field peas to be used in Zn biofortification programs, all combinations of soil Zn application of 0, 4 and 8mgZnSO4·7H2Okg(-1) and foliar Zn application of 0 and two sprays of 0.25% or 0.5% (w/v) ZnSO4·7H2O before flowering and at early grain-filling stage were tested. Soil Zn application increased Zn-DTPA concentration 3.7- to 5.6-times depending on the Zn soil treatments. Grain Zn concentrations higher than 60mgZnkg(-1) were obtained with all foliar Zn applications, alone or in combination with soil Zn applications, and grain Zn bioavailability was adequate (phytate:Zn ratios lower than 15). Processing (freezing and cooking) caused a decrease of about 30% in grain Zn concentration and a 17%-increase in phytate:Zn ratios (to ⩽9.5). The combined application of 8mgZnSO4·7H2Okg(-1) soil+0.25% (w/v) ZnSO4·7H2O foliarly could be a good option for biofortifying field peas.
Asunto(s)
Biofortificación/métodos , Culinaria/métodos , Grano Comestible/química , Pisum sativum , Suelo/química , Sulfato de Zinc/análisis , Disponibilidad Biológica , Grano Comestible/metabolismo , Fertilizantes/análisis , Sulfato de Zinc/administración & dosificación , Sulfato de Zinc/metabolismoRESUMEN
Vast agricultural areas are affected by flooding, causing up to 80% yield reduction and resulting in multibillion dollar losses. Up to now, the focus of plant breeders was predominantly on detrimental effects of anoxia, while other (potentially equally important) traits were essentially neglected; one of these is soil elemental toxicity. Excess water triggers a progressive decrease in soil redox potential, thus increasing the concentration of Mn(2+) that can be toxic to plants if above a specific threshold. This work aimed to quantify the relative contribution of Mn(2+) toxicity to waterlogging stress tolerance, using barley as a case study. Twenty barley (Hordeum vulgare) genotypes contrasting in waterlogging stress tolerance were studied for their ability to cope with toxic (1 mm) amounts of Mn(2+) in the root rhizosphere. Under Mn(2+) toxicity, chlorophyll content of most waterlogging-tolerant genotypes (TX9425, Yerong, CPI-71284-48 and CM72) remained above 60% of the control value, whereas sensitive genotypes (Franklin and Naso Nijo) had 35% less chlorophyll than 35% of controls. Manganese concentration in leaves was not related to visual Mn(2+) toxicity symptoms, suggesting that various Mn(2+) tolerance mechanisms might operate in different tolerant genotypes, i.e. avoidance versus tissue tolerance. The overall significant (r = 0.60) correlation between tolerance to Mn(2+) toxicity and waterlogging in barley suggests that plant breeding for tolerance to waterlogging traits may be advanced by targeting mechanisms conferring tolerance to Mn(2+) toxicity, at least in this species.
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Hordeum/fisiología , Manganeso/toxicidad , Agua/fisiología , Biomasa , Cruzamiento , Clorofila/metabolismo , Genotipo , Hordeum/efectos de los fármacos , Hordeum/crecimiento & desarrollo , Hidroponía , Fenotipo , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/fisiología , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/fisiología , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/crecimiento & desarrollo , Brotes de la Planta/fisiología , Rizosfera , Suelo/química , Estrés FisiológicoRESUMEN
Calmodulin (CaM), a small acidic protein, is one of the best characterised Ca(2+) sensors in eukaryotes. This Ca(2+) -regulated protein plays a critical role in decoding and transducing environmental stress signals by activating specific targets. Many environmental stresses elicit changes in intracellular Ca(2+) activity that could initiate adaptive responses under adverse conditions. We report the first molecular cloning and characterisation of a calmodulin gene, VcCaM1 (Vaccinium corymbosum Calmodulin 1), in the woody shrub, highbush blueberry. VcCaM1 was first identified as VCAL19, a gene induced by aluminium stress in V. corymbosum L. A full-length cDNA of VcCaM1 containing a 766-bp open reading frame (ORF) encoding 149 amino acids was cloned from root RNA. The sequence encodes four Ca(2+) -binding motifs (EF-hands) and shows high similarity (99%) with the isoform CaM 201 of Daucus carota. Expression analyses showed that following Al treatment, VcCaM1 message level decreased in roots of Brigitta, an Al-resistant cultivar, and after 48 h, was lower than in Bluegold, an Al-sensitive cultivar. VcCAM1 message also decreased in leaves of both cultivars within 2 h of treatment. Message levels in leaves then increased by 24 h to control levels in Brigitta, but not in Bluegold, but then decreased again by 48 h. In conclusion, VcCaM1 does not appear to be directly involved in Al resistance, but may be involved in improved plant performance under Al toxicity conditions through regulation of Ca(2+) homeostasis and antioxidant systems in leaves.
Asunto(s)
Aluminio/toxicidad , Arándanos Azules (Planta)/genética , Calmodulina/genética , Regulación de la Expresión Génica de las Plantas , Estrés Fisiológico , Arándanos Azules (Planta)/efectos de los fármacos , Arándanos Azules (Planta)/fisiología , Calmodulina/metabolismo , ADN Complementario/genética , Especificidad de Órganos , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/genética , Hojas de la Planta/fisiología , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/genética , Raíces de Plantas/fisiología , ARN de Planta/genética , Alineación de Secuencia , Análisis de Secuencia de ADNRESUMEN
Crop nutrition is frequently inadequate as a result of the expansion of cropping into marginal lands, elevated crop yields placing increasing demands on soil nutrient reserves, and environmental and economic concerns about applying fertilizers. Plants exposed to nutrient deficiency activate a range of mechanisms that result in increased nutrient availability in the rhizosphere compared with the bulk soil. Plants may change their root morphology, increase the affinity of nutrient transporters in the plasma membrane and exude organic compounds (carboxylates, phenolics, carbohydrates, enzymes, etc.) and protons. Chemical changes in the rhizosphere result in altered abundance and composition of microbial communities. Nutrient-efficient genotypes are adapted to environments with low nutrient availability. Nutrient efficiency can be enhanced by targeted breeding through pyramiding efficiency mechanisms in a desirable genotype as well as by gene transfer and manipulation. Rhizosphere microorganisms influence nutrient availability; adding beneficial microorganisms may result in enhanced availability of nutrients to crops. Understanding the role of plant-microbe-soil interactions in governing nutrient availability in the rhizosphere will enhance the economic and environmental sustainability of crop production.
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Micorrizas/crecimiento & desarrollo , Micorrizas/genética , Cruzamiento , Ecosistema , Genotipo , Manganeso/metabolismo , Fósforo/metabolismo , Raíces de Plantas/metabolismo , Raíces de Plantas/microbiología , Plantas Comestibles/crecimiento & desarrollo , Plantas Comestibles/metabolismo , Plantas Comestibles/microbiología , Suelo/análisisRESUMEN
Arsenic is highly toxic and therefore represents a potential threat to the environment and human health. The mobility and bioavailability of arsenic in soil is mostly controlled by adsorption and desorption reactions. Even though adsorption and traditional batch desorption experiments provide information about the environmental fate of As, the equilibrium conditions imposed in these studies would usually not be reached in the natural environment. Flow-through desorption techniques, where the desorbed species are removed from the substrate, can therefore be used to provide information about the rate and mechanisms of As desorption. The effect of pH on As adsorption reactions is relatively well understood; however, desorption of As and the effect of pH on As desorption remain unexplored. Desorption of As(V) (the most dominant arsenic species in aerated soils) was therefore investigated using batch and flow-through desorption experiments. Traditional batch desorption experiments underestimated the desorption rate of As(V) from kaolinite. The pH had a large effect on the amount of As(V) desorbed from kaolinite, with both an increase and a decrease in pH (from the initial pH 6.4) enhancing As(V) desorption. Modeling desorption over time revealed that the pH can influence As(V) desorption over extended periods of time.
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Arseniatos/química , Arsénico/química , Caolín/química , Modelos Teóricos , Contaminantes del Suelo/análisis , Adsorción , Arsénico/análisis , Concentración de Iones de Hidrógeno , CinéticaRESUMEN
A critical review of the literature on Al toxicity in plants, animals and humans reveals a similar mode of Al action in all living organisms, namely interference with the secondary messenger system (phosphoinositide and cytosolic Ca2+ signalling pathways) and enhanced production of reactive oxygen species resulting in oxidative stress. Aluminium uptake by plants is relatively quick (across the intact plasma membrane in < 30 min and across the tonoplast in < 1 h), despite huge proportion of Al being bound in the cell wall. Aluminium absorption in the animal/human digestive system is low (only about 0.1% of daily Al intake stays in the human body), except when Al is complexed with organic ligands (eg. citrate, tartarate, glutamate). Aluminium accumulates in bones and brain, with Al-citrate and Al-transferrin complexes crossing the blood-brain barrier and accumulating in brain cells. Tea plant and other Al-accumulator plant species contain large amounts of Al in the form of non-toxic organic complexes.
Asunto(s)
Aluminio/metabolismo , Animales , Calcio/metabolismo , Pared Celular , Cromatografía , Cromatografía Líquida de Alta Presión , Humanos , Ligandos , Neuronas/metabolismo , Estrés Oxidativo , Fenómenos Fisiológicos de las Plantas , Plantas/metabolismo , Especies Reactivas de Oxígeno , Transducción de Señal , Suelo , Té , Factores de TiempoRESUMEN
Little is known about root architectural attributes that aid the capture of nitrate from coarse-textured soil profiles of high leaching potential. In this study, a range of root architectures from the herringbone to the dichotomous structure were simulated, and their capacity to take up nitrate leaching through a sandy profile was recorded. All root systems had equal total volume at each point in time, and so were considered cost equivalent. These simulations showed that the root architecture likely to maximize nitrate capture from sandy soils (under the Mediterranean rainfall pattern experienced in Western Australia) is one that quickly produces a high density of roots in the top-soil early in the season, thereby reducing total nitrate leached with opening season rains, but also has vigorous taproot growth, enabling access to deep-stored water and leached nitrate later in the season. This is the first published, spatially explicit attempt to assess the ability of different root architectures equivalent in cost, to capture nitrate from a spatially and temporally heterogeneous soil environment.
RESUMEN
This review is concentrating on the role of aluminium (Al)-calcium (Ca) interactions in Al toxicity syndrome in plants. Disruption of cytoplasmic Ca2+ homeostasis has been suggested as a primary trigger of Al toxicity. Aluminium causes an increase in cytosolic Ca2+ activity, potentially disrupting numerous biochemical and physiological processes, including those involved in the root growth. The source of Ca2+ for the increase in cytosolic Ca2+ activity under Al exposure is partly extracellular (likely to be due to the Al-resistant portion of the flux through depolarization-activated Ca2+ channels and fluxes through Ca2+ -permeable nonselective cation channels in the plasma membrane) as well as intracellular (increased cytosolic Ca2+ activity enhances the activity of Ca2+ release channels in the tonoplast and the endoplasmic reticulum membrane). The effect on increased cytosolic Ca2+ activity of possible Al-related inhibition of the plasma membrane and endo-membrane Ca2+ -ATPases and Ca2+ exchangers (CaX) that sequester Ca2+ out of the cytosol is insufficiently documented at present. The relationship between Al toxicity, cytoplasmic Ca2+ homeostasis and cytoplasmic pH needs to be elucidated. Technical improvements that would allow measurements of cytosolic Ca2+ activity within the short time after exposure to Al (seconds or shorter) are eagerly awaited. Contents I. Introduction 296 II. Symptoms of aluminium toxicity 296 III. Calcium - aluminium interactions 297 IV. The role of electrical properties of the plasma membrane in calcium-aluminium interactions 306 V. Oxidative stress 307 VI. Callose 308 VII. Cytoskeleton 308 VIII. Conclusions 309 Acknowledgements 309 References 309.
RESUMEN
Nitrate uptake, nitrate reductase activity (NRA) and net proton release were compared in five grain legumes grown at 0.2 and 2 mM nitrate in nutrient solution. Nitrate treatments, imposed on 22-d-old, fully nodulated plants, lasted for 21 d. Increasing nitrate supply did not significantly influence the growth of any of the species during the treatment, but yellow lupin (Lupinus luteus) had a higher growth rate than the other species examined. At 0.2 mM nitrate supply, nitrate uptake rates ranged from 0.6 to 1.5 mg N g(-1) d(-1) in the order: yellow lupin > field pea (Pisum sativum) > chickpea (Cicer arietinum) > narrow-leafed lupin (L angustifolius) > white lupin (L albus). At 2 mM nitrate supply, nitrate uptake ranged from 1.7 to 8.2 mg N g(-1) d(-1) in the order: field pea > chickpea > white lupin > yellow lupin > narrow-leafed lupin. Nitrate reductase activity increased with increased nitrate supply, with the majority of NRA being present in shoots. Field pea and chickpea had much higher shoot NRA than the three lupin species. When 0.2 mM nitrate was supplied, narrow-leafed lupinreleased the most H+ per unit root biomass per day, followed by yellow lupin, white lupin, field pea and chickpea. At 2 mM nitrate, narrow-leafed lupin and yellow lupin showed net proton release, whereas the other species, especially field pea, showed net OH- release. Irrespective of legume species and nitrate supply, proton release was negatively correlated with nitrate uptake and NRA in shoots, but not with NRA in roots.
Asunto(s)
Fabaceae/enzimología , Fabaceae/metabolismo , Nitrato Reductasas/metabolismo , Nitratos/metabolismo , Protones , Fabaceae/crecimiento & desarrollo , Nitrato-Reductasa , Nitrógeno/análisis , Nitrógeno/metabolismo , Tamaño de los Órganos , Raíces de Plantas/enzimología , Raíces de Plantas/metabolismo , Brotes de la Planta/enzimología , Brotes de la Planta/metabolismo , Especificidad de la EspecieRESUMEN
The effect of colonization with the arbuscular mycorrhizal (AM) fungus Glomus mosseae (Nicol. & Gerd.) Gerdemann & Trappe on the growth and physiology of NaCl-stressed maize plants ( Zea mays L. cv. Yedan 13) was examined in the greenhouse. Maize plants were grown in sand with 0 or 100 mM NaCl and at two phosphorus (P) (0.05 and 0.1 mM) levels for 34 days, following 34 days of non-saline pre-treatment. Mycorrhizal plants maintained higher root and shoot dry weights. Concentrations of chlorophyll, P and soluble sugars were higher than in non-mycorrhizal plants under given NaCl and P levels. Sodium concentration in roots or shoots was similar in mycorrhizal and non-mycorrhizal plants. Mycorrhizal plants had higher electrolyte concentrations in roots and lower electrolyte leakage from roots than non-mycorrhizal plants under given NaCl and P levels. Although plants in the low P plus AM fungus treatment and those with high P minus AM fungus had similar P concentrations, the mycorrhizal plants still had higher dry weights, soluble sugars and electrolyte concentrations in roots. Similar relationships were observed regardless of the presence or absence of salt stress. Higher soluble sugars and electrolyte concentrations in mycorrhizal plants suggested a higher osmoregulating capacity of these plants. Alleviation of salt stress of a host plant by AM colonization appears not to be a specific effect. Furthermore, higher requirement for carbohydrates by AM fungi induces higher soluble sugar accumulation in host root tissues, which is independent of improvement in plant P status and enhances resistance to salt-induced osmotic stress in the mycorrhizal plant.
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Micorrizas/fisiología , Raíces de Plantas/microbiología , Zea mays/microbiología , Hongos/fisiología , Fósforo , Raíces de Plantas/fisiología , Cloruro de Sodio , Zea mays/crecimiento & desarrollo , Zea mays/fisiologíaRESUMEN
In symbiotically-grown legumes, rhizosphere acidification may be caused by a high cation/anion uptake ratio and the excretion of organic acids, the relative importance of the two processes depending on the phosphorus nutritional status of the plants. The present study examined the effect of P deficiency on extrusions of H(+) and organic acid anions (OA(-)) in relation to uptake of excess cations in N(2)-fixing white lupin (cv. Kiev Mutant). Plants were grown for 49 days in nutrient solutions treated with 1, 5 or 25 mmol P m(-3) Na(2)HPO(4) in a phytotron room. The increased formation of cluster roots occurred prior to a decrease in plant growth in response to P deficiency. The number of cluster roots was negatively correlated with tissue P concentrations below 2.0 g kg(-1) in shoots and 3 g kg(-1) in roots. Cluster roots generally had higher concentrations of Mg, Ca, N, Cu, Fe, and Mn but lower concentrations of K than non-cluster roots. Extrusion of protons and OA(-) (90% citrate and 10% malate) from roots was highly dependent on P supply. The amounts of H(+) extruded per unit root biomass decreased with time during the experiment. On the equimolar basis, H(+) extrusion by P-deficient plants (grown at 1 and 5 mmol P m(-3)) were, on average, 2-3-fold greater than OA(-) exudation. The excess cation content in plants was generally the highest at 1 mmol P m(-3) and decreased with increasing P supply. The ratio of H(+) release to excess cation uptake increased with decreasing P supply. The results suggest that increased exudation of OA(-) due to P deficiency is associated with H(+) extrusion but contributes only a part of total acidification.
RESUMEN
Quantitative information on the uptake and distribution of Al at the cellular level is required to understand mechanisms of Al toxicity, but direct measurement of uptake across the plasma membrane has remained elusive. We measured rates of Al transport across membranes in single cells of Chara corallina using the rare (26)Al isotope, an emerging technology (accelerator mass spectrometry), and a surgical technique for isolating subcellular compartments. Accumulation of Al in the cell wall dominated total uptake (71-318 microgram m(-2) min(-1)), although transport across the plasma membrane was detectable (71-540 ng m(-2) min(-1)) within 30 min of exposure. Transport across the tonoplast was initially negligible, but accelerated to rates approximating uptake across the plasma membrane. The avacuolate protoplasm showed signs of saturation after 60 min, but continued movement across the plasma membrane was supported by sequestration in the vacuole. Saturation of all compartments was observed after 12 to 24 h. Accumulation of Al in the cell wall reflected variation in [Al(3+)] induced by changes in Al supply or complexing ligands, but was unaffected by pH. In contrast, transport across the plasma membrane peaked at pH 4.3 and increased when [Al(3+)] was reduced by complexing ligands. Cold temperature (4 degrees C) reduced accumulation in the cell wall and protoplasm, whereas 2,4-dinitrophenol and m-chlorocarbonylcyanidephenyl hydrazone increased membrane transport by 12- to 13-fold. Our data suggest that the cell wall is the major site of Al accumulation. Nonetheless, membrane transport occurs within minutes of exposure and is supported by subsequent sequestration in the vacuole. The rapid delivery of Al to the protoplasm suggests that intracellular lesions may be possible.
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Aluminio/metabolismo , Eucariontes/metabolismo , 2,4-Dinitrofenol/farmacología , Aluminio/toxicidad , Transporte Biológico , Membrana Celular/metabolismo , Pared Celular/metabolismo , Células Cultivadas , Frío , Hidrazonas/farmacología , Concentración de Iones de Hidrógeno , Vacuolas/metabolismoRESUMEN
The pho2 mutant of Arabidopsis thaliana (L.) Heynh. accumulates excessive Pi (inorganic phosphate) concentrations in shoots compared to wild-type plants (E. Delhaize and P. Randall, 1995, Plant Physiol. 107: 207-213). In this study, a series of experiments was conducted to compare the uptake and translocation of Pi by pho2 with that of wild-type plants. The pho2 mutants had about a twofold greater Pi uptake rate than wild-type plants under P-sufficient conditions and a greater proportion of the Pi taken up accumulated in shoots of pho2. When shoots were removed, the uptake rate by roots was found to be similar for both genotypes, suggesting that the greater Pi uptake by the intact pho2 mutant is due to a greater shoot sink for Pi. Although pho2 mutants could recycle 32Pi from shoots to roots through phloem the proportion of 32Pi translocated to roots was less than half of that found in wild-type plants. When transferred from P-sufficient to P-deficient solutions, Pi concentrations in pho2 roots had a similar depletion rate to wild-type roots despite pho2 shoots having a fourfold greater Pi concentration than wild-type shoots throughout the experiment. We suggest that the pho2 phenotype could result from a partial defect in Pi transport in the phloem between shoots and roots or from an inability of shoot cells to regulate internal Pi concentrations.
Asunto(s)
Arabidopsis/metabolismo , Organofosfatos/metabolismo , Proteínas de Plantas/metabolismo , Arabidopsis/genética , Mutación , Radioisótopos de Fósforo , Proteínas de Plantas/genética , Raíces de Plantas/metabolismoRESUMEN
Calcium ions serve as a second messenger in signal transduction and metabolic regulation. Effects of Al on calcium homeostasis remain to be elucidated. Short-term net (45)Ca(2+) uptake by Amaranthus tricolor protoplasts was monitored from uptake media prepared to test the influence of pH, Al, and various inhibitors. Accumulation of (45)Ca(2+) increased during the first 3 to 6 minutes and then leveled off or declined. Al and Ca(2+) channel blockers (verapamil and bepridil) decreased net (45)Ca(2+) uptake. This decrease was more pronounced when Al and bepridil were both present in uptake media, but Al did not aggravate verapamil-induced reduction of net (45)Ca(2+) uptake. Erythrosin B and calmidazolium each increased net (45)Ca(2+) uptake, probably by interfering with Ca(2+) efflux. This effect was undetectable in the presence of Al. Mycophenolic acid decreased net (45)Ca(2+) uptake; guanosine alleviated this effect. Al-induced reduction of net (45)Ca(2+) uptake was not aggravated by mycophenolic acid. Net (45)Ca(2+) uptake was generally less at pH 4.5 than at 5.5 for all treatments. It is concluded that Al ions affect net (45)Ca(2+) uptake by binding to the verapamil-specific channel site that is different from the bepridil-specific one, as well as by interfering with the action of guanosine 5'-triphosphate-binding proteins.
RESUMEN
Rhizotoxicity of Al is more pronounced in younger plants. Effects of Al on nutrient uptake by plants of different age are poorly understood. The depletion technique was used to monitor net Mg(2+) uptake from nutrient solutions by intact 15- and 35-day-old plants of two ryegrass (Lolium multiflorum Lam.) cultivars. Lowering the pH from 6.0 to 4.2 decreased the maximum net ion influx without affecting K(m). Aluminum at 6.6 micromolar Al(3+) activity increased K(m) indicating competitive inhibition. The effects of pH and 6.6 micromolar Al(3+) on net Mg(2+) uptake were much larger in 15- than in 35-day-old plants. Aluminum at 26 micromolar Al(3+) activity competitively inhibited net Mg(2+) uptake by 35-day-old plants, while causing time- and external Mg(2+) activity-dependent net Mg(2+) efflux from 15-day-old plants. The equilibrium constant (K(i)) of a reversible combination of postulated plasmalemma Mg(2+) transporter and Al(3+) was calculated to be 2 and 5 micromolar Al(3+) activity for 15-day-old plants of Wilo and Gulf ryegrass, respectively, and 21 micromolar Al(3+) activity for 35-day-old plants of both cultivars. The Al(3+)-mediated increase in K(m) was larger for 15-day-old plants of the Al-sensitive cultivar ;Wilo' than of the more Al-tolerant cultivar ;Gulf,' while Al(3+) affected 35-day-old plants of both cultivars to the same extent.
RESUMEN
Aluminum impairs uptake of Mg(2+), but the mechanisms of this inhibition are not understood. The depletion technique was used to monitor net Mg(2+) uptake from nutrient solution by intact, 23-day-old plants of ryegrass (Lolium multiflorum Lam., cv Gulf and Wilo). Activities of Mg(2+) and monomeric Al species in nutrient solution were calculated and used as the basis for expressing the results. The kinetics of net Mg(2+) absorption was resolved into (a) a transpiration-dependent uptake component, (b) a metabolically mediated, discontinuous saturable component that is Al(3+) sensitive and p-chloromercuribenzene sulfonic acid (PCMBS) resistant, and (c) a linear, carbonyl cyanide m-chlorophenylhydrazone resistant, Al(3+) sensitive component that might be a type of facilitated diffusion. Lowering the pH from 6.0 to 4.2 exerted a noncompetitive inhibition of net Mg(2+) uptake, while aluminum at 6.6 micromolar Al(3+) activity exerted competitive inhibition of net Mg(2+) uptake at pH 4.2. The Al(3+)-induced effect was obvious after 30 minutes. Cultivar-specific ability to retain a higher affinity for Mg(2+) by postulated transport proteins in the presence of Al(3+) might be one of the mechanisms of differential Al tolerance among ryegrass cultivars.
RESUMEN
Callus tissue was induced on barley mesocotyl explants of germinated seven-day-old seedlings on MS medium supplemented with 2,4-D or 2,4,5-T in high concentrations. Two morphologically different tissue cultures were maintained in vitro for a long time: a callus tissue without organogenesis and a culture with high rhizogenic capacity. Shoots and plantlets were generated when the auxin-media induced callus was transferred to medium supplemented with 3 µM TIBA. In 62% of cultures, during the first five subcultures, four to twentyeight plants per single mesocotyl were obtained. Some cultures produced shoots even in the 9th subculture, being in culture for nearly 14 months. The largest number of plants obtained per one mesocotyl was forty. Plantlets rooted well on MS with 5.7 µM IAA and survived transplantation to soil in high percentages.