Rumen fermentation and metabolic profile of rams fed with diets amended cottonseed cake.

The aim of the study was to evaluate the addition of increasing cottonseed cake contents in the diet. The diets were composed of corn silage (500 g kg-1) and concentrate feed (500 g kg-1) on a dry matter (DM) basis. The treatments consisted of the substitution of 0, 140, 280, and 420 g kg-1 soybean meal to cottonseed cake in the DM of concentrate feed. Four rams of the Santa Inês breed, average age of 12 months and average weight of 27.77 ± 3.87 kg, were distributed in a Latin square design (4 × 4) in a split-split-plot design, with diets in the plots and as subplots were 5 days of collection and the collection times. The results were subjected to analysis of variance and regression at 5% probability. The soybean meal substitution contents by cottonseed cake in the concentrate increased linearly the pH (P = 0.019). Ruminal ammonia-nitrogen concentration decreased linearly by 0.0137 mg dL-1 at each 10 g/kg of substitution and the time after feeding promoted the same effect (P < 0.001) decreased linearly by 0.6204 mg dL-1 at each hour after feeding. There was an increased linearly of 0.0116 mmol mL-1 at the concentration of propionic (P = 0.008) and a reduction of 0.0062 mmol mL-1 at the concentration of butyric (P = 0.009) with cottonseed cake content; however, the substitution did not influence the others short-chain fatty acids. Replacement of soybean meal with cottonseed cake in the sheep feed did not significantly alter the blood parameters, promoting minor changes in the ruminal parameters mainly in the ruminal ammonia-nitrogen without affecting the animal's health.

Bean coproduct as source of protein in dairy cows diets / Coproduto do feijão como fonte proteica em dietas para vacas leiteiras

The objective of this study was to evaluate the supplementation of dairy cows with different protein sources in relation to intake, digestibility, production and chemical composition of milk. For this, eight crossbred cows (Hostein x Gir) were used, distributed in two simultaneous latin squares (4x4). The treatments used were: bean coproduct, soybean meal, sunflower meal and cottonseed meal. Four variance-covariance structures were tested by means of the Akaike criterion corrected to determine the best fit to the data, and afterwards they were submitted to analysis of variance, and the means of the treatments were compared by the Tukey's test, at 5% probability. Only intakes of crude protein and crude fat were influenced by the treatments, for the digestibility, regardless of the evaluated nutrient, the soybean meal was the best treatment or was among the best, a behavior also observed for milk production. Thus, it was concluded that soybean meal is the best source of protein among the supplements used, and the bean coproduct stands out as a low-cost protein source but should not fully replace soybean meal, especially in animal categories of higher nutritional requirement.

Objetivou-se com este estudo avaliar a suplementação de vacas leiteiras com diferentes fontes proteicas em relação ao consumo, digestibilidade, produção e composição química do leite. Para tanto, utilizou-se oito vacas mestiças (Holandês x Gir), distribuídas em dois quadrados latinos (4x4) simultâneos. Os tratamentos utilizados foram: coproduto do feijão, farelo de soja, farelo de girassol e farelo de algodão. Foram testadas quatro estruturas de variância-covariância por meio do critério de Akaike corrigido para determinar o melhor ajuste aos dados, e posteriormente foram submetidas à análise de variância, sendo as médias dos tratamentos comparadas pelo teste de Tukey, a 5% de probabilidade. Apenas os consumos de proteína bruta e gordura bruta foram influenciados pelos tratamentos, já em relação a digestibilidade, independente do nutriente avaliado o farelo de soja foi o melhor tratamento ou esteve entre os melhores, comportamento este observado também para a produção de leite. Com isso, concluiu-se que dentre os suplementos utilizados o farelo de soja é a melhor fonte proteica, e o coproduto de feijão se destaca como uma fonte proteica de baixo custo, mas que deve substituir integralmente o farelo de soja, principalmente em categorias animais com alta exigência nutricional.

Effects of Streptococcus bovis Isolated from Bovine Rumen on the Fermentation Characteristics and Nutritive Value of Tanzania Grass Silage.

This study aimed to evaluate the effects of Streptococcus bovis on the fermentation characteristics and nutritive value of Tanzania grass silage. Tanzania grass was chopped and left untreated (U) or treated with Streptococcus bovis JB1 at 1 × 10(6) colony-forming units per gram (cfu/g) of fresh forage or Streptococcus bovis HC5 at 1 × 10(6) cfu/g of fresh forage and packed into sixtuplicate laboratory silos. The largest number of enterobacteria, molds and yeast (M&Y) occurred in untreated silages and the smallest populations of enterobacteria and M&Y and the largest numbers of lactic acid bacteria (LAB), at 9.81 and 9.87 log cfu/g, were observed in Streptococcus bovis JB1 and HC5, respectively (P < 0.05). Silages treated with JB1 and HC5 had lower (P < 0.05) silage pHs and concentrations of ammoniacal nitrogen (NH3-N) than untreated silages. The application of Streptococcus bovis JB1 and HC5 resulted in fewer losses through gases and effluents (P < 0.05), which resulted in greater dry matter recovery (DMR) and crude protein recovery (CPR) (P < 0.05). Streptococcus bovis JB1 and HC5 improved the fermentative profile and increased the concentration of crude protein and DMR and CPR in Tanzania grass silage.

Blood parameters of grazing dairy cows supplemented with different protein sources / Parâmetros sanguíneos de vacas leiteiras mantidas em pasto suplementadas com diferentes fontes proteicas

Objetivou-se com este trabalho avaliar o efeito da suplementação com diferentes fontes proteicas na dieta de vacas leiteiras mantidas em pastagem de Brachiaria brizantha MG5 sobre os níveis séricos de glicose, triglicerídeos, colesterol e ureia e das enzimas gama glutamina transferase, creatina quinase e aminotransferase. Avaliaram-se diferentes fontes proteicas: coproduto de feijão, farelo de soja, farelo de girassol e farelo de algodão. Foram utilizadas 8 vacas cruzadas 5/8 (Holandês x Gir), peso médio 500±60,33kg, produção média de leite de 14,85±3,28kg/dia, entre 60 e 90 dias de lactação, em delineamento quadrado latino 4 x 4 duplo e simultâneo. Cada período experimental teve duração de 15 dias, sendo 10 dias iniciais para adaptação dos animais e os demais para avaliação e coleta de dados. Houve efeito de interação (P0,05) das fontes proteicas para os valores de triglicerídeos, colesterol e ureia dos animais, com média de 6,46±2,05; 182,43±37,52 e 38,20±12,29mg/dL, respectivamente. Houve efeito (P0,05) os níveis séricos das enzimas gama glutamina transferase, creatina quinase e aminotransferase, com médias de 26,76±8,02; 112,13±31,72 e 58,79±20,13 UI/L, respectivamente. As fontes proteicas promoveram alterações na glicemia com os tempos de coletas, sendo que estes alteraram os valores de triglicerídeos e ureia. Não foram observadas alterações para os demais metabólitos

The present work aimed to evaluate the effect of supplementation with different protein sources in the diets for dairy cows kept on Brachiaria brizantha MG5 grass pasture on serum levels of the glucose, triglycerides, cholesterol and urea as well as on enzymes gama grutamil transferase, creatinine quinase and aminotransferase. We have evaluated the following protein sources: bean byproduct, soybean meal, sunflower meal and cottonseed meal. Eight crossbred cows (Holstein x Gir) were used, weighing 500±60.33 kg, average milk production of 14.85±3.28 kg/day, between the 60th and the 90th day of calving, by a simultaneous double 4 x 4 latin square design 4 x 4. Each experimental period lasted 15 days, of which the first 10 days were designed to animal adaptation and the final five days for data collection. There was an interaction effect (P 0.05) among protein sources and sampling time on serum glucose. There was no effect (P > 0.05) of protein sources for the triglycerides, cholesterol and urea levels, averaging 6.46±2.05, 182.43±37.52 and 38.20±12.29 mg/dL, respectively. There was a significant effect (P 0.05) of the sampling time on blood levels of triglycerides and urea. The different protein sources did not affect significantly (P > 0.05) serum enzyme levels of glutamine transferase, creatinine quinase and aminotransferase, averaging 26.76±8.02, 112.13±20.13

Blood parameters of grazing dairy cows supplemented with different protein sources / Parâmetros sanguíneos de vacas leiteiras mantidas em pasto suplementadas com diferentes fontes proteicas

Objetivou-se com este trabalho avaliar o efeito da suplementação com diferentes fontes proteicas na dieta de vacas leiteiras mantidas em pastagem de Brachiaria brizantha MG5 sobre os níveis séricos de glicose, triglicerídeos, colesterol e ureia e das enzimas gama glutamina transferase, creatina quinase e aminotransferase. Avaliaram-se diferentes fontes proteicas: coproduto de feijão, farelo de soja, farelo de girassol e farelo de algodão. Foram utilizadas 8 vacas cruzadas 5/8 (Holandês x Gir), peso médio 500±60,33kg, produção média de leite de 14,85±3,28kg/dia, entre 60 e 90 dias de lactação, em delineamento quadrado latino 4 x 4 duplo e simultâneo. Cada período experimental teve duração de 15 dias, sendo 10 dias iniciais para adaptação dos animais e os demais para avaliação e coleta de dados. Houve efeito de interação (P0,05) das fontes proteicas para os valores de triglicerídeos, colesterol e ureia dos animais, com média de 6,46±2,05; 182,43±37,52 e 38,20±12,29mg/dL, respectivamente. Houve efeito (P0,05) os níveis séricos das enzimas gama glutamina transferase, creatina quinase e aminotransferase, com médias de 26,76±8,02; 112,13±31,72 e 58,79±20,13 UI/L, respectivamente. As fontes proteicas promoveram alterações na glicemia com os tempos de coletas, sendo que estes alteraram os valores de triglicerídeos e ureia. Não foram observadas alterações para os demais metabólitos(AU)

The present work aimed to evaluate the effect of supplementation with different protein sources in the diets for dairy cows kept on Brachiaria brizantha MG5 grass pasture on serum levels of the glucose, triglycerides, cholesterol and urea as well as on enzymes gama grutamil transferase, creatinine quinase and aminotransferase. We have evaluated the following protein sources: bean byproduct, soybean meal, sunflower meal and cottonseed meal. Eight crossbred cows (Holstein x Gir) were used, weighing 500±60.33 kg, average milk production of 14.85±3.28 kg/day, between the 60th and the 90th day of calving, by a simultaneous double 4 x 4 latin square design 4 x 4. Each experimental period lasted 15 days, of which the first 10 days were designed to animal adaptation and the final five days for data collection. There was an interaction effect (P 0.05) among protein sources and sampling time on serum glucose. There was no effect (P > 0.05) of protein sources for the triglycerides, cholesterol and urea levels, averaging 6.46±2.05, 182.43±37.52 and 38.20±12.29 mg/dL, respectively. There was a significant effect (P 0.05) of the sampling time on blood levels of triglycerides and urea. The different protein sources did not affect significantly (P > 0.05) serum enzyme levels of glutamine transferase, creatinine quinase and aminotransferase, averaging 26.76±8.02, 112.13±20.13(AU)

Caroço de algodão em dietas sem volumoso para cordeiros confinados / Whole cottonseed in diets without roughage for feedlot lambs

Objetivou-se avaliar o desempenho, consumo, comportamento ingestivo, os custos de produção e os parâmetros nutricionais de ovinos alimentados com dietas contendo diferentes teores de caroço de algodão - zero, 10, 20, 30 e 40%. Foram realizados dois experimentos, em que no primeiro foi usado um Delineamento Inteiramente Casualisado, com cinco tratamentos e seis repetições. Os animais foram mantidos em baias coletivas. O experimento teve duração de 70 dias, sendo os animais pesados no início e final do experimento. No segundo experimento, foram usados cinco animais por meio de quadrado latino 5x5, divididos em cinco períodos de 14 dias, totalizando 70 dias experimentais. Os consumos MS, MO, PB, EE, FDN e CNF foram influenciados de forma quadrática (P 0,01) pelos níveis de caroço de algodão na dieta. Os níveis de caroço de algodão tiveram efeito quadrático ( 0,01) apenas sobre a digestibilidade do EE, não sendo observado efeito sobre os demais nutrientes (P>0,05). Não foram observados efeitos (P>0,05) dos níveis de caroço de algodão nas dietas sobre o ganho médio diário, sendo obtido valor médio de 200,8 g/animal/dia, bem como sobre o comportamento ingestivo dos animais (P>0,05). Foram encontradas margens brutas de R$ 0,18 e 0,19 por animal/dia, respectivamente para os níveis 10 e 20% de caroço de algodão. Recomenda-se a inclusão de até 20% de caroço de algodão...(AU)

The objective of this work was to evaluate the performance, intake, feeding behavior, costs of production and nutritional parameters of sheep fed diets containing different levels of whole cottonseed (WC): zero, 10, 20, 30 and 40%. Two experiments have been carried out, in which in the first trial we have used a completely randomized design with five treatments and six replicates each, which lasted 70 days. The animals were kept in collective stalls and all of them have been weighed at the beginning and end of the experiment. The second experiment was a 5x5 Latin square design with five treatments and five animals divided in five periods of 14 days, totaling 70 days of experimentation. The intakes of the DM, OM, EE, NDF and NFC ave been not affected by WC levels (P>0.01). The WC levels had quadratic affect only on EE digestibility (P 0.01) and there were no observed effects on digestibility for other nutrients (P>0.05). There were no effects (P>0.05) of whole cottonseed on average daily gain with average of 200.8g/animal/day, as well as there was no effect of the WC levels on intake behavior. A gross margins of R$ 0.18 to 0.19 per animal / day was observed for WC levels of 10 to 20%.Thus, it was concluded that whole cottonseed can be included in lambs diets up 20% of dry matter basis in which roughage has been not used.(AU)

Caroço de algodão em dietas sem volumoso para cordeiros confinados / Whole cottonseed in diets without roughage for feedlot lambs

Objetivou-se avaliar o desempenho, consumo, comportamento ingestivo, os custos de produção e os parâmetros nutricionais de ovinos alimentados com dietas contendo diferentes teores de caroço de algodão - zero, 10, 20, 30 e 40%. Foram realizados dois experimentos, em que no primeiro foi usado um Delineamento Inteiramente Casualisado, com cinco tratamentos e seis repetições. Os animais foram mantidos em baias coletivas. O experimento teve duração de 70 dias, sendo os animais pesados no início e final do experimento. No segundo experimento, foram usados cinco animais por meio de quadrado latino 5x5, divididos em cinco períodos de 14 dias, totalizando 70 dias experimentais. Os consumos MS, MO, PB, EE, FDN e CNF foram influenciados de forma quadrática (P 0,01) pelos níveis de caroço de algodão na dieta. Os níveis de caroço de algodão tiveram efeito quadrático ( 0,01) apenas sobre a digestibilidade do EE, não sendo observado efeito sobre os demais nutrientes (P>0,05). Não foram observados efeitos (P>0,05) dos níveis de caroço de algodão nas dietas sobre o ganho médio diário, sendo obtido valor médio de 200,8 g/animal/dia, bem como sobre o comportamento ingestivo dos animais (P>0,05). Foram encontradas margens brutas de R$ 0,18 e 0,19 por animal/dia, respectivamente para os níveis 10 e 20% de caroço de algodão. Recomenda-se a inclusão de até 20% de caroço de algodão...

The objective of this work was to evaluate the performance, intake, feeding behavior, costs of production and nutritional parameters of sheep fed diets containing different levels of whole cottonseed (WC): zero, 10, 20, 30 and 40%. Two experiments have been carried out, in which in the first trial we have used a completely randomized design with five treatments and six replicates each, which lasted 70 days. The animals were kept in collective stalls and all of them have been weighed at the beginning and end of the experiment. The second experiment was a 5x5 Latin square design with five treatments and five animals divided in five periods of 14 days, totaling 70 days of experimentation. The intakes of the DM, OM, EE, NDF and NFC ave been not affected by WC levels (P>0.01). The WC levels had quadratic affect only on EE digestibility (P 0.01) and there were no observed effects on digestibility for other nutrients (P>0.05). There were no effects (P>0.05) of whole cottonseed on average daily gain with average of 200.8g/animal/day, as well as there was no effect of the WC levels on intake behavior. A gross margins of R$ 0.18 to 0.19 per animal / day was observed for WC levels of 10 to 20%.Thus, it was concluded that whole cottonseed can be included in lambs diets up 20% of dry matter basis in which roughage has been not used.

Effects of substituting soybean meal for sunflower cake in the diet on the growth and carcass traits of crossbred boer goat kids.

The present study was conducted to determine the best level of substitution of soybean meal by sunflower cake in diets for kids through the evaluation of quantitative carcass traits. Thirty-two Boer kids X 1/2 NDB (no defined breed), males, non-castrated, with 4 months of age and initial body weight of 15±3.2 kg, were randomly assigned to individual pens. The treatments contained four substitution levels of soybean meal by sunflower cake (0, 33, 66 and 100% DM). At the end of the experimental period, the animals were slaughtered. There was no influence of the treatments on any of the mean values of the evaluated measures (p>0.05): 21.78 kg (body weight at slaughter), 8.65 kg (hot carcass weight), 8.59 kg (cold carcass weight), 40.27% (hot carcass yield), 39.20% (cold carcass yield), 7.73 cm(2)(rib eye area), 46.74 cm (carcass outer length), 45.68 cm (carcass internal length), 36.92 cm (leg length), 26.04 cm (leg perimeter), 48.66 cm (hind perimeter), 58.62 cm (thoracic perimeter), 0.20 (carcass compactness index), 68.48% (total muscle of the leg), 2.79% (total leg fat), 55.19% (subcutaneous leg fat), 28.82% (total bone), 81.66 g (femur weight), 14.88 cm (femur length), 0.38 (leg muscularity index), 2.53 (muscle:bone ratio) and 33.42 (muscle:fat ratio). The substitution of soybean meal by sunflower cake may be recommended up to a level of 100% without alterations to quantitative carcass traits.

Silagem de capim-elefante aditivada com produtos alternativos / Elephant grass silage with added alternative product

Objetivou-se avaliar o padrão de fermentação e a composição bromatológica da silagem de capim-elefante utilizando diferentes aditivos. Utilizou-se o delineamento inteiramente casualizado com seis tratamentos (capim-elefante sem aditivo; com 100 kg de farelo de arroz t-1 de massa verde (MV); com 100 kg de casca de soja t-1 MV; com 100 kg de fubá de milho t-1 MV; com 200 kg de cana-de-açúcar picada t-1 MV; com inoculante bacteriano) e quatro repetições. Utilizaram-se silos de PVC, adotando densidade de 600 kg de massa verde m-3. Com a exceção do inoculante bacteriano, todos os aditivos proporcionaram aumento nos teores de matéria seca (27,95 a 31,65%) e carboidratos solúveis (15,20 a 17,14%) na forragem a ser ensilada. Os aditivos promoveram adequado padrão de fermentação face aos valores de pH (3,70 a 3,96) e de nitrogênio amoniacal (3,67 a 4,44% do N-total) verificados na silagem. O aditivo farelo de arroz proporcionou a obtenção de silagens com teores mais elevados de proteína bruta (6,41%) e nutrientes digestíveis totais (61,91%) em relação ao tratamento sem aditivo.

The objective was to evaluate the fermentation pattern and chemical composition of elephant grass silage using different additives. The experimental design was completely randomized with six treatments (elephant grass no additive; with 100 kg of rice bran ton-1 of green mass (GM); with 100 kg of soybean hulls ton-1 GM; with 100 kg of corn meal ton-1 GM; with 200 kg of sugar cane ton-1 GM; with inoculants) and four replications. PVC silos were used, adopting a density of 600 kg of green mass m-3. With the exception of inoculant, all additives used resulted in increased dry matter content (27.95 to 31.65%) and soluble carbohydrate contents (15.20 to 17.14%) in the forage being ensiled. The additives promoted an adequate fermentation pattern for pH values (3.70 to 3.96) and ammonia nitrogen (3.67 to 4.44% of total N) observed in the silage. The rice bran additive yielded silage with higher contents of crude protein (6.41%) and total digestible nutrients (61.91%) compared to the treatment without any additive.

Consumo e digestibilidade aparente de minerais em dietas para cäes contendo polpa de citros e folha de alfafa / Intake and apparent digestibility of the minerals in canine diets containing citrus pulp and alfalfa leaf

O consumo diário e a digestibilidade aparente de macro e microminerais, foram determinados em cadelas que receberam uma dieta controle (R0), acrescida de 10 (R10) ou 20 por cento (R20) de uma mistura contendo polpa de citros (PC) e folhas de alfafa desidratadas. A digestibilidade aparente dos minerais foi estimada por meio da quantidade do elemento mineral ingerido, subtraída daquela eliminada nas fezes. A medida que a ingestäo de fibra dietética aumentou, o consumo de MS (em g/kgPV) diminuiu e as digestibilidades aparentes do Ca, P, Mg, Na, Zn, Fe e Cu foram maiores. A exceçäo foi para o Mn, cuja digestibilidade reduziu-se com o aumento da ingestäo de fibra. Apenas para o K, näo se verificou alteraçäo na sua digestibilidade em funçäo do nível de fibra na dieta