RESUMEN
Adsorption of antibodies against antigens expressed on the Candida albicans blastoconidia cell walls was standardized for the detection of antibodies to germ tubes by an indirect immunofluorescence test (IIFT). Sera from rabbits immunized with C. albicans bearing germ tubes, were diluted by two-fold serial dilutions to obtain specimens 1 : 640-1 : 20 positive by IIFT. Different volumes of specimens were adsorbed with different amounts of whole, heat-inactivated C. albicans blastoconidia. It was found that a 1 : 640 titre serum should be adsorbed at 30 microl by 50 mg cells, down to a 1 : 20 titre serum at 110 microl by 12.5 mg cells. Accurate detection of anti-germ tube antibodies by IIFT depends on the semi-quantitative relation between the positive titre of the specimen and the amount of blastoconidia used in adsorption.
Asunto(s)
Anticuerpos Antifúngicos/sangre , Candida albicans/crecimiento & desarrollo , Candida albicans/inmunología , Pared Celular/inmunología , Técnica del Anticuerpo Fluorescente Indirecta , Adsorción , Animales , Antígenos Fúngicos/inmunología , Antígenos Fúngicos/fisiología , Candidiasis/inmunología , Candidiasis/microbiología , Inmunización , ConejosRESUMEN
Enterotoxic Escherichia coli (ETEC) having a gene for thermolabile toxin were identified by the hybridization technique. HindIII-SmaI fragment with 423 basic pairs (dp) including subunit portions A and B of the gene for thermolabile toxin (elt) was used as the DNA probe.
Asunto(s)
Sondas de ADN , Enterotoxinas/biosíntesis , Escherichia coli/clasificación , Enterotoxinas/genética , Escherichia coli/genética , Escherichia coli/metabolismoRESUMEN
The quantities of DNA and RNA were measured in rat liver homogenate, supernatant and nuclei after scalding. Maximal increases of nuclear DNA and RNA were found 12 h after thermal injury. The RNA content in the rat liver supernatant and homogenate reached its maximum at 24 and 48 h, respectively. The results obtained in this study show that the thermal injury resulted in an activation of the genetic apparatus in rat liver cells. These findings correspond well with a significant role of the liver in post-traumatic metabolism.
