RESUMEN
Understanding how social experiences are represented in the brain and shape future responses is a major challenge in the study of behavior. We addressed this problem by studying behavioral, transcriptomic and epigenetic responses to intrusion in honey bees. Previous research showed that initial exposure to an intruder provokes an immediate attack; we now show that this also leads to longer-term changes in behavior in the response to a second intruder, with increases in the probability of responding aggressively and the intensity of aggression lasting 2 and 1 h, respectively. Previous research also documented the whole-brain transcriptomic response; we now show that in the mushroom bodies (MBs) there are 2 waves of gene expression, the first highlighted by genes related to cytoskeleton remodeling, and the second highlighted by genes related to hormones, stress response and transcription factors (TFs). Overall, 16 of 37 (43%) of the TFs whose cis-motifs were enriched in the promoters of the differentially expressed genes (DEGs) were also predicted from transcriptional regulatory network analysis to regulate the MB transcriptional response, highlighting the strong role played by a relatively small subset of TFs in the MB's transcriptomic response to social challenge. Whole brain histone profiling showed few changes in chromatin accessibility in response to social challenge; most DEGs were 'ready' to be activated. These results show how biological embedding of a social challenge involves temporally dynamic changes in the neurogenomic state of a prominent region of the insect brain that are likely to influence future behavior.
Asunto(s)
Abejas/genética , Epigénesis Genética , Genes de Insecto , Conducta Social , Transcriptoma , Animales , Abejas/fisiología , Encéfalo/metabolismo , Cromatina/genética , Cromatina/metabolismo , Citoesqueleto/metabolismo , Redes Reguladoras de Genes , Histonas/genética , Histonas/metabolismoRESUMEN
The discovery of toolkit genes, which are highly conserved genes that consistently regulate the development of similar morphological phenotypes across diverse species, is one of the most well-known observations in the field of evolutionary developmental biology. Surprisingly, this phenomenon is also relevant for a wide array of behavioral phenotypes, despite the fact that these phenotypes are highly complex and regulated by many genes operating in diverse tissues. In this chapter, we review the use of the toolkit concept in the context of behavior, noting the challenges of comparing behaviors and genes across diverse species, but emphasizing the successes in identifying genetic toolkits for behavior; these successes are largely attributable to the creative research approaches fueled by advances in behavioral genomics. We have two general goals: (1) to acknowledge the groundbreaking progress in this field, which offers new approaches to the difficult but exciting challenge of understanding the evolutionary genetic basis of behaviors, some of the most complex phenotypes known, and (2) to provide a theoretical framework that encompasses the scope of behavioral genetic toolkit studies in order to clearly articulate the research questions relevant to the toolkit concept. We emphasize areas for growth and highlight the emerging approaches that are being used to drive the field forward. Behavioral genetic toolkit research has elevated the use of integrative and comparative approaches in the study of behavior, with potentially broad implications for evolutionary biologists and behavioral ecologists alike.
Asunto(s)
Encéfalo/fisiología , Redes Reguladoras de Genes , Genética Conductual/métodos , Animales , Conducta Animal , Evolución Molecular , Ontología de Genes , Genes , Genómica/métodos , Fenotipo , Análisis de Secuencia de ADN/métodos , Transducción de Señal/genética , Transducción de Señal/fisiología , Especificidad de la EspecieRESUMEN
The ability of honey bees to evaluate differences in food type and value is crucial for colony success, but these assessments are made by individuals who bring food to the hive, eating little, if any, of it themselves. We tested the hypothesis that responses to food type (pollen or nectar) and value involve different subsets of brain regions, and genes responsive to food. mRNA in situ hybridization of c-jun revealed that brain regions responsive to differences in food type were mostly different from regions responsive to differences in food value, except those dorsal and lateral to the mushroom body calyces, which responded to all three. Transcriptomic profiles of the mushroom bodies generated by RNA sequencing gave the following results: (1) responses to differences in food type or value included a subset of molecular pathways involved in the response to food reward; (2) genes responsive to food reward, food type and food value were enriched for (the Gene Ontology categories) mitochondrial and endoplasmic reticulum activity; (3) genes responsive to only food and food type were enriched for regulation of transcription and translation; and (4) genes responsive to only food and food value were enriched for regulation of neuronal signaling. These results reveal how activities necessary for colony survival are channeled through the reward system of individual honey bees.
Asunto(s)
Abejas/fisiología , Animales , Abejas/genética , Abejas/metabolismo , Encéfalo/metabolismo , Fenómenos Fisiológicos Celulares , Alimentos , Redes Reguladoras de Genes , Cuerpos Pedunculados/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , RecompensaRESUMEN
Immediate early genes (IEGs) have served as useful markers of brain neuronal activity in mammals, and more recently in insects. The mammalian canonical IEG, c-jun, is part of regulatory pathways conserved in insects and has been shown to be responsive to alarm pheromone in honey bees. We tested whether c-jun was responsive in honey bees to another behaviourally relevant stimulus, sucrose, in order to further identify the brain regions involved in sucrose processing. To identify responsive regions, we developed a new method of voxel-based analysis of c-jun mRNA expression. We found that c-jun is expressed in somata throughout the brain. It was rapidly induced in response to sucrose stimuli, and it responded in somata near the antennal and mechanosensory motor centre, mushroom body calices and lateral protocerebrum, which are known to be involved in sucrose processing. c-jun also responded to sucrose in somata near the lateral suboesophageal ganglion, dorsal optic lobe, ventral optic lobe and dorsal posterior protocerebrum, which had not been previously identified by other methods. These results demonstrate the utility of voxel-based analysis of mRNA expression in the insect brain.
Asunto(s)
Abejas/metabolismo , Genes Inmediatos-Precoces , Genes jun , Sacarosa/farmacología , Animales , Abejas/genética , Encéfalo/metabolismo , Cuerpos Pedunculados/metabolismo , Especificidad de ÓrganosRESUMEN
Aerobic glycolysis involves increased glycolysis and decreased oxidative catabolism of glucose even in the presence of an ample oxygen supply. Aerobic glycolysis, a common metabolic pattern in cancer cells, was recently discovered in both the healthy and diseased human brain, but its functional significance is not understood. This metabolic pattern in the brain is surprising because it results in decreased efficiency of adenosine triphosphate (ATP) production in a tissue with high energetic demands. We report that highly aggressive honey bees (Apis mellifera) show a brain transcriptomic and metabolic state consistent with aerobic glycolysis, i.e. increased glycolysis in combination with decreased oxidative phosphorylation. Furthermore, exposure to alarm pheromone, which provokes aggression, causes a metabolic shift to aerobic glycolysis in the bee brain. We hypothesize that this metabolic state, which is associated with altered neurotransmitter levels, increased glycolytically derived ATP and a reduced cellular redox state, may lead to increased neuronal excitability and oxidative stress in the brain. Our analysis provides evidence for a robust, distinct and persistent brain metabolic response to aggression-inducing social cues. This finding for the first time associates aerobic glycolysis with naturally occurring behavioral plasticity, which has important implications for understanding both healthy and diseased brain function.
Asunto(s)
Agresión/fisiología , Conducta Animal , Encéfalo/metabolismo , Glucólisis/fisiología , Adenosina Trifosfato/metabolismo , Animales , Abejas , Glucosa/metabolismo , Análisis por Micromatrices/métodos , FeromonasRESUMEN
The social environment plays an essential role in shaping behavior for most animals. Social effects on behavior are often linked to changes in brain gene expression. In the honey bee (Apis mellifera L.), social modulation of individual aggression allows colonies to adjust the intensity with which they defend their hive in response to predation threat. Previous research has showed social effects on both aggression and aggression-related brain gene expression in honey bees, caused by alarm pheromone and unknown factors related to colony genotype. For example, some bees from less aggressive genetic stock reared in colonies with genetic predispositions toward increased aggression show both increased aggression and more aggressive-like brain gene expression profiles. We tested the hypothesis that exposure to a colony environment influenced by high levels of predation threat results in increased aggression and aggressive-like gene expression patterns in individual bees. We assessed gene expression using four marker genes. Experimentally induced predation threats modified behavior, but the effect was opposite of our predictions: disturbed colonies showed decreased aggression. Disturbed colonies also decreased foraging activity, suggesting that they did not habituate to threats; other explanations for this finding are discussed. Bees in disturbed colonies also showed changes in brain gene expression, some of which paralleled behavioral findings. These results show that bee aggression and associated molecular processes are subject to complex social influences.
Asunto(s)
Agresión , Abejas/fisiología , Encéfalo/fisiología , Genes de Insecto , Proteínas de Insectos/metabolismo , Medio Social , Animales , Abejas/genética , Abejas/metabolismo , Conducta Animal , Encéfalo/metabolismo , Proteínas de Insectos/genética , Conducta PredatoriaRESUMEN
Vitellogenin (Vg) is best known as a yolk protein precursor. Vg also functions to regulate behavioural maturation in adult honey bee workers, but the underlying molecular mechanisms by which it exerts this novel effect are largely unknown. We used abdominal vitellogenin (vg) knockdown with RNA interference (RNAi) and brain transcriptomic profiling to gain insights into how Vg influences honey bee behavioural maturation. We found that vg knockdown caused extensive gene expression changes in the bee brain, with much of this transcriptional response involving changes in central biological functions such as energy metabolism. vg knockdown targeted many of the same genes that show natural, maturation-related differences, but the direction of change for the genes in these two contrasts was not correlated. By contrast, vg knockdown targeted many of the same genes that are regulated by juvenile hormone (JH) and there was a significant correlation for the direction of change for the genes in these two contrasts. These results indicate that the tight coregulatory relationship that exists between JH and Vg in the regulation of honey bee behavioural maturation is manifest at the genomic level and suggest that these two physiological factors act through common pathways to regulate brain gene expression and behaviour.
Asunto(s)
Abejas/genética , Conducta Animal , Química Encefálica/genética , Proteínas de Insectos/deficiencia , Vitelogeninas/deficiencia , Animales , Abejas/metabolismo , Dieta , Femenino , Regulación de la Expresión Génica/genética , Técnicas de Silenciamiento del Gen , Proteínas de Insectos/genética , Proteínas de Insectos/metabolismo , Hormonas Juveniles/deficiencia , Hormonas Juveniles/genética , Hormonas Juveniles/metabolismo , Masculino , Vías Nerviosas/metabolismo , Vías Nerviosas/fisiología , Interferencia de ARN , Vitelogeninas/genética , Vitelogeninas/metabolismoRESUMEN
Small, non-coding microRNAs (miRNAs) have been implicated in many biological processes, including the development of the nervous system. However, the roles of miRNAs in natural behavioral and neuronal plasticity are not well understood. To help address this we characterized the microRNA transcriptome in the adult worker honey bee head and investigated whether changes in microRNA expression levels in the brain are associated with division of labor among honey bees, a well-established model for socially regulated behavior. We determined that several miRNAs were downregulated in bees that specialize on brood care (nurses) relative to foragers. Additional experiments showed that this downregulation is dependent upon social context; it only occurred when nurse bees were in colonies that also contained foragers. Analyses of conservation patterns of brain-expressed miRNAs across Hymenoptera suggest a role for certain miRNAs in the evolution of the Aculeata, which includes all the eusocial hymenopteran species. Our results support the intriguing hypothesis that miRNAs are important regulators of social behavior at both developmental and evolutionary time scales.
Asunto(s)
Abejas/genética , Conducta Animal/fisiología , Química Encefálica/genética , MicroARNs/genética , Plasticidad Neuronal/genética , Transcriptoma/genética , Envejecimiento/genética , Animales , Abejas/fisiología , Evolución Biológica , Química Encefálica/fisiología , Femenino , Masculino , FilogeniaRESUMEN
Worker honey bees (Apis mellifera) undergo a process of behavioral maturation leading to their transition from in-hive tasks to foraging--a process which is associated with profound transcriptional changes in the brain. Changes in brain gene expression observed during worker behavioral maturation could represent either a derived program underlying division of labor or a general program unrelated to sociality. Male bees (drones) undergo a process of behavioral maturation associated with the onset of mating flights, but do not partake in division of labor. Drones thus provide an excellent reference point for polarizing transcriptional changes associated with behavioral maturation in honey bees. We assayed the brain transcriptomes of adult drones and workers to compare and contrast differences associated with behavioral maturation in the two sexes. Both behavioral maturation and sex were associated with changes in expression of thousands of genes in the brain. Many genes involved in neuronal development, behavior, and the biosynthesis of neurotransmitters regulating the perception of reward showed sex-biased gene expression. Furthermore, most of the transcriptional changes associated with behavioral maturation were common to drones and workers, consistent with common genetic and physiological regulation. Our study suggests that there is a common behavioral maturation program that has been co-opted and modified to yield the different behavioral and cognitive phenotypes of worker and drone bees.
Asunto(s)
Envejecimiento/genética , Abejas/genética , Conducta Alimentaria/fisiología , Transcripción Genética/fisiología , Animales , Química Encefálica/genética , Conducta Exploratoria/fisiología , Femenino , Regulación del Desarrollo de la Expresión Génica/genética , Masculino , Análisis de Secuencia por Matrices de Oligonucleótidos , Caracteres Sexuales , Conducta Social , Especificidad de la EspecieRESUMEN
Deeply conserved molecular mechanisms regulate food-searching behaviour in response to nutritional cues in a wide variety of vertebrates and invertebrates. Studies of the highly eusocial honey bee have shown that nutritional physiology and some conserved nutrient signalling pathways, especially the insulin pathway, also regulate the division of labour between foraging and non-foraging individuals. Typically, lean workers leave the nest to forage for food, and well-nourished workers perform tasks inside the nest. Here we provide the first direct test of whether similar mechanisms operate in a primitively eusocial insect in an independently evolved social lineage, the paper wasp Polistes metricus. We found that food deprivation caused reduced lipid stores and higher levels of colony and individual foraging. Individuals with greatly reduced lipid stores foraged at extremely elevated levels. In addition, brain expression of several foraging-related genes was influenced by food deprivation, including insulin-like peptide 2 (ilp2). Together with previous findings, our results demonstrate that nutrition regulates foraging division of labour in two independently evolved social insect lineages (bees and wasps), despite large differences in social organization. Our results also provide additional support for the idea that nutritional asymmetries among individuals, based on differences in nutritional physiology and expression of conserved nutrient signalling genes in the brain, are important in the division of labour in eusocial societies.
Asunto(s)
Conducta Apetitiva/fisiología , Encéfalo/metabolismo , Transcriptoma , Avispas/genética , Animales , Regulación de la Expresión Génica , Estado Nutricional , Análisis de Secuencia de ADN , Transducción de SeñalRESUMEN
Previous research has led to the idea that derived traits can arise through the evolution of novel roles for conserved genes. We explored whether neuropeptide Y (NPY)-like signalling, a conserved pathway that regulates food-related behaviour, is involved in a derived, nutritionally-related trait, the division of labour in worker honey bees. Transcripts encoding two NPY-like peptides were expressed in separate populations of brain neurosecretory cells, consistent with endocrine functions. NPY-related genes were upregulated in the brains of older foragers compared with younger bees performing brood care ('nurses'). A subset of these changes can be attributed to nutrition, but neuropeptide F peptide treatments did not influence sugar intake. These results contrast with recent reports of more robust associations between division of labour and the related insulin-signalling pathway and suggest that some elements of molecular pathways associated with feeding behaviour may be more evolutionarily labile than others.
Asunto(s)
Abejas/genética , Abejas/fisiología , Conducta Alimentaria , Expresión Génica , Neuropéptido Y/genética , Neuropéptido Y/metabolismo , Animales , Abejas/metabolismo , Evolución Biológica , Encéfalo/citología , Encéfalo/metabolismo , Miel , Neuropéptidos/genética , Neuropéptidos/metabolismo , Fenotipo , Transducción de Señal/genética , Conducta Social , Regulación hacia Arriba/genéticaRESUMEN
We report that regions of the honey bee brain involved in visual processing and learning and memory show a specific genomic response to distance information. These results were obtained with an established method that separates effects of perceived distance from effects of actual distance flown. Individuals forced to shift from a short to perceived long distance to reach a feeding site showed gene expression differences in the optic lobes and mushroom bodies relative to individuals that continued to perceive a short distance, even though they all flew the same distance. Bioinformatic analyses suggest that the genomic response to distance information involves learning and memory systems associated with well-known signaling pathways, synaptic remodeling, transcription factors and protein metabolism. By showing distance-sensitive brain gene expression, our findings also significantly extend the emerging paradigm of the genome as a dynamic regulator of behavior, that is particularly responsive to stimuli important in social life.
Asunto(s)
Comunicación Animal , Abejas/genética , Abejas/fisiología , Conducta Animal/fisiología , Encéfalo/fisiología , Percepción de Distancia/genética , Percepción de Distancia/fisiología , Animales , Química Encefálica/genética , Química Encefálica/fisiología , ADN/genética , Metabolismo Energético/fisiología , Expresión Génica/fisiología , Cuerpos Pedunculados/metabolismo , Análisis de Secuencia por Matrices de Oligonucleótidos , Análisis de Componente Principal , Control de Calidad , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Studies of genes and social behavior, aided by new genomic resources, are coming of age. Here, we show how some of the insights that have emerged from research on the evolution of development (evo-devo) also provide a useful framework for studying the evolution of social behavior at the molecular level. These insights include co-opting old genes for new functions, phenotypic modularity, genetic tool kits, the importance of gene regulation in evolutionary change, and the influences of some genes over multiple timescales. We next outline a few differences between development and behavior that pose challenges for an evo-devo approach to behavior. For the remainder of this chapter, we review several studies that illustrate the relevance of evo-devo insights to our understanding of the evolution of behaviors related to eusociality in the insect societies.
Asunto(s)
Evolución Biológica , Encéfalo/fisiología , Insectos/genética , Insectos/fisiología , Conducta Social , Animales , Femenino , Expresión Génica , Genes de Insecto , Masculino , Modelos GenéticosRESUMEN
Pheromones are very important in animal communication. To learn more about the molecular basis of pheromone action, we studied the effects of a potent honey bee pheromone on brain gene expression. Brood pheromone (BP) caused changes in the expression of hundreds of genes in the bee brain in a manner consistent with its known effects on behavioral maturation. Brood pheromone exposure in young bees causes a delay in the transition from working in the hive to foraging, and we found that BP treatment tended to upregulate genes in the brain that are upregulated in bees specialized on brood care but downregulate genes that are upregulated in foragers. However, the effects of BP were age dependent; this pattern was reversed when older bees were tested, consistent with the stimulation of foraging by BP in older bees already competent to forage. These results support the idea that one way that pheromones influence behavior is by orchestrating large-scale changes in brain gene expression. We also found evidence for a relationship between cis and BP regulation of brain gene expression, with several cis-regulatory motifs statistically overrepresented in the promoter regions of genes regulated by BP. Transcription factors that target a few of these motifs have already been implicated in the regulation of bee behavior. Together these results demonstrate strong connections between pheromone effects, behavior, and regulation of brain gene expression.
Asunto(s)
Abejas/genética , Encéfalo/metabolismo , Regulación de la Expresión Génica , Feromonas/metabolismo , Olfato/genética , Secuencias de Aminoácidos/genética , Animales , Abejas/citología , Conducta Animal/fisiología , Encéfalo/citología , Regulación hacia Abajo/genética , Conducta Alimentaria/fisiología , Femenino , Regiones Promotoras Genéticas/genética , Elementos Reguladores de la Transcripción/genética , Conducta Social , Regulación hacia Arriba/genéticaRESUMEN
Carbohydrate-metabolizing enzymes may have particularly interesting roles in the honey bee, Apis mellifera, because this social insect has an extremely carbohydrate-rich diet, and nutrition plays important roles in caste determination and socially mediated behavioural plasticity. We annotated a total of 174 genes encoding carbohydrate-metabolizing enzymes and 28 genes encoding lipid-metabolizing enzymes, based on orthology to their counterparts in the fly, Drosophila melanogaster, and the mosquito, Anopheles gambiae. We found that the number of genes for carbohydrate metabolism appears to be more evolutionarily labile than for lipid metabolism. In particular, we identified striking changes in gene number or genomic organization for genes encoding glycolytic enzymes, cellulase, glucose oxidase and glucose dehydrogenases, glucose-methanol-choline (GMC) oxidoreductases, fucosyltransferases, and lysozymes.
Asunto(s)
Abejas/genética , Metabolismo de los Hidratos de Carbono/genética , Genoma de los Insectos , Animales , Celulasa/genética , Drosophila/genética , Ácidos Grasos/metabolismo , Fucosiltransferasas/genética , Glucosa 1-Deshidrogenasa/genética , Glucosa Oxidasa/genética , Muramidasa/genética , Oxidorreductasas/genéticaRESUMEN
Antioxidant enzymes perform a variety of vital functions including the reduction of life-shortening oxidative damage. We used the honey bee genome sequence to identify the major components of the honey bee antioxidant system. A comparative analysis of honey bee with Drosophila melanogaster and Anopheles gambiae shows that although the basic components of the antioxidant system are conserved, there are important species differences in the number of paralogs. These include the duplication of thioredoxin reductase and the expansion of the thioredoxin family in fly; lack of expansion of the Theta, Delta and Omega GST classes in bee and no expansion of the Sigma class in dipteran species. The differential expansion of antioxidant gene families among honey bees and dipteran species might reflect the marked differences in life history and ecological niches between social and solitary species.
Asunto(s)
Antioxidantes , Abejas/genética , Genes de Insecto , Secuencia de Aminoácidos , Animales , Anopheles/genética , Catalasa/genética , Drosophila melanogaster/genética , Expresión Génica , Genes Bacterianos , Glutarredoxinas , Glutatión Peroxidasa/genética , Glutatión Transferasa/genética , Metionina Sulfóxido Reductasas , Datos de Secuencia Molecular , Oxidorreductasas/genética , Peroxidasas/genética , Peroxirredoxinas , Superóxido Dismutasa/genética , Reductasa de Tiorredoxina-Disulfuro/genética , Tiorredoxinas/genéticaRESUMEN
Transcription is slow relative to many post-transcriptional processes in the brain. Using the rich system of division of labor in the honeybee (Apis mellifera), we found extreme differences in the extent to which behavioral occupations of different durations were associated with gene-expression differences in the brain. Nursing and foraging, occupations lasting > 1 week, were associated with significant expression differences for nearly one-quarter of the genes tested (1208 of 5563 cDNAs tested; P < 0.01, anova), consistent with previous results. In contrast, transitional occupations, performed for 1-2 days after nursing and before the onset of foraging, were associated with either no differences (guards vs. undertakers; 19 cDNAs, fewer than the expectation of 56 false-positives) or few differences (comb builders vs. guards and undertakers; 248 cDNAs), but extensive differences relative to both nursing and foraging (> 500 cDNAs, all contrasts). Statistical power analysis indicated that expression differences of two-, 1.5- and 1.25-fold should have been detected in 100, 92 and 37% of cases, respectively. Replication of previous results at these magnitudes was 95, 71 and 51%, with no genes showing differences in the opposite direction. These results indicate that behavioral plasticity over different time-scales may be associated with substantial differences in the extent of genomic plasticity in the brain.
Asunto(s)
Abejas/genética , Abejas/fisiología , Conducta Animal/fisiología , Química Encefálica/genética , Animales , ADN Complementario/biosíntesis , ADN Complementario/genética , Regulación de la Expresión Génica , Análisis de Secuencia por Matrices de Oligonucleótidos , Reproducibilidad de los Resultados , Transcripción GenéticaRESUMEN
Changes in circadian rhythms of behavior are related to age-based division of labor in honey bee colonies. The expression of the clock gene period (per) in the bee brain is associated with age-related changes in circadian rhythms of behavior, but previous efforts to firmly associate per brain expression with division of labor or age have produced variable results. We explored whether this variability was due to differences in light and flight experience, which vary with division of labor, or differences in colony environment, which are known to affect honey bee behavioral development. Our results support the hypothesis that per mRNA expression in the bee brain is developmentally regulated. One-day-old bees had the lowest levels of expression and rarely showed evidence of diurnal fluctuation, while foragers and forager-age bees (> 21 days of age) always had high levels of brain per and strong and consistent diurnal patterns. Results from laboratory and field experiments do not support the hypothesis that light, flight experience, and colony type influence per expression. Our results suggest that the rate of developmental elevation in per expression is influenced by factors other than the ones studied in our experiments, and that young bees are more sensitive to these factors than foragers.
Asunto(s)
Abejas/crecimiento & desarrollo , Abejas/fisiología , Proteínas de Insectos/biosíntesis , Proteínas de Insectos/genética , Proteínas Nucleares/biosíntesis , Proteínas Nucleares/genética , Animales , Encéfalo/metabolismo , Ritmo Circadiano , Conducta Alimentaria , Vuelo Animal/fisiología , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Luz , Actividad Motora/fisiología , Proteínas Circadianas Period , ARN Mensajero/biosíntesis , Conducta SocialRESUMEN
The behavioral maturation of adult worker honey bees is influenced by a rising titer of juvenile hormone (JH), and is temporally correlated with an increase in the volume of the neuropil of the mushroom bodies, a brain region involved in learning and memory. We explored the stability of this neuropil expansion and its possible dependence on JH. We studied the volume of the mushroom bodies in adult bees deprived of JH by surgical removal of the source glands, the corpora allata. We also asked if the neuropil expansion detected in foragers persists when bees no longer engage in foraging, either because of the onset of winter or because colony social structure was experimentally manipulated to cause some bees to revert from foraging to tending brood (nursing). Results show that adult exposure to JH is not necessary for growth of the mushroom body neuropil, and that the volume of the mushroom body neuropil in adult bees is not reduced if foraging stops. These results are interpreted in the context of a qualitative model that posits that mushroom body neuropil volume enlargement in the honey bee has both experience-independent and experience-dependent components.