RESUMEN
Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also affect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in different plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identified OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on different tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profiling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1 and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to define candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location.
Asunto(s)
Ácido Graso Desaturasas/genética , Oxigenasas de Función Mixta/genética , Olea/genética , Ácidos Grasos/análisis , Ácidos Grasos Insaturados , Frutas/genética , Perfilación de la Expresión Génica/métodos , Regulación de la Expresión Génica de las Plantas/genética , Genes de Plantas/genética , Olea/metabolismo , Ácido Oléico , Reguladores del Crecimiento de las Plantas/genética , Reguladores del Crecimiento de las Plantas/metabolismo , Proteínas de Plantas/genéticaRESUMEN
Heat shock proteins 90 (HSP90) are essential and play critical roles in the adaptation of organisms to diverse stimuli. In plants, HSP90 are involved in auxin, jasmonate and brassinosteroid (BR) signalling pathways. The BR-promoted activation of the BES1 transcription factor regulates BR-responsive genes. Using genetic, physiological, fluorescence live cell imaging, molecular and biochemical approaches, such as phenotypic analysis, co-immunoprecipitation assay, yeast-two hybrid and Bimolecular fluorescence complementation (BiFC), we studied complex formation between BES1 and HSP90 under control conditions and active BR signalling. Further, we determined the effect of the pharmacological inhibition of HSP90 ATPase activity on hypocotyl elongation of bes1-D mutant. We determined that HSP90 interact with BES1 in the nucleus and in the cytoplasm. During active BR signalling, nuclear complexes were absent while cytoplasmic HSP90/BES1 complexes were prominent. Our results showed that the hypocotyl length of bes1-D mutants was highly reduced when HSP90 was challenged by the geldanamycin (GDA) inhibitor of the ATPase activity of HSP90. Active BR signalling could not rescue the GDA effect on the hypocotyl elongation of bes1-D. Our results reveal that the constitutively active BES1 in the bes1-D mutant is hypersensitive to GDA. The interaction of HSP90 with BES1 argues that HSP90 facilitate the nuclear metastable conformation of BES1 to regulate BR-dependent gene expression, and our data show that HSP90 assist in the compartmentalised cycle of BES1 during active BR signalling.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Brasinoesteroides , Proteínas HSP90 de Choque Térmico , Transducción de Señal , Proteínas de Arabidopsis/metabolismo , Brasinoesteroides/metabolismo , Proteínas de Unión al ADN/metabolismo , Regulación de la Expresión Génica de las Plantas , Proteínas HSP90 de Choque Térmico/metabolismo , Nicotiana/metabolismoRESUMEN
In the present study soluble fibrin complexes and fibrin(ogen) degradation products are determined in patients with sepsis and liver-cirrhotic simultaneously by means of FM-Test, FDP-Kit and SCT in order to detect the different and early phases of disseminated intravascular coagulation. According to the possible configuration of test results 43 patients (sepsis n = 23, liver cirrhotic n = 20) could be grouped in 4 phases. The FDP-concentrations and the FM-Test-result appear to be independent from one another. A positive FM-Test was found at least once in 35% of patients with liver-cirrhotic and 26% of patients with sepsis. The correlation coefficient for the two FDP-Tests (FDP-Kit and SCT) was r = 0.97 (p < 0.001) for liver-cirrhotic and r = 0.98 (p < 0.001) for sepsis.
Asunto(s)
Coagulación Intravascular Diseminada/sangre , Productos de Degradación de Fibrina-Fibrinógeno/análisis , Cirrosis Hepática/sangre , Choque Séptico/sangre , Coagulación Intravascular Diseminada/clasificación , Coagulación Intravascular Diseminada/diagnóstico , Humanos , Cirrosis Hepática/diagnóstico , Juego de Reactivos para Diagnóstico , Valores de Referencia , Choque Séptico/diagnósticoRESUMEN
Amidolytic chromogenic substrate assays are frequently used to determine the anticoagulant activities of various commercial heparins. With the help of a combined assay method heparin characterization is made possible using the TAT/XAT quotient under consideration of the simultaneous inhibition of the two serine proteases thrombin and factor Xa by antithrombin III. The test is primarily designed for qualitative characterization where a numerical value can be assigned to every heparin. However, quantitative aspects may also be evaluated.
Asunto(s)
Amidas/metabolismo , Heparina de Bajo-Peso-Molecular/farmacología , Heparina/farmacología , Antitrombina III/metabolismo , Antitrombinas/metabolismo , Calibración , Humanos , Péptido Hidrolasas/metabolismoRESUMEN
In a prospective study in severely traumatized patients, procoagulant activity (PCA) was determined in bronchoalveolar lavage fluids (BAL). Bronchoscopy with lavage was serially performed during the first 15 days after injury (in total 148 samples of 25 patients). PCA was measured as recalcification times in the absence or presence of excess phosphatidylethanolamine and translated into procoagulant unit equivalents using standard thromboplastin. The data were correlated to the extent of respiratory failure in the injured patients and were compared to PCA in 29 lavage samples obtained from 10 healthy control subjects. A several-fold increase in BAL PCA was noted in all trauma victims, evident already within the first 24 h after injury. A progressive rise in PCA occurred from the 4th posttraumatic day and was highly significantly more pronounced in patients developing serious respiratory failure than in those with only mild pulmonary dysfunction. Significant correlations were noted between PCA increase and alveolar protein-leakage, granulocyte-influx and surfactant alterations, however with correlation coefficients not surpassing 0.55. We conclude that a marked increase in procoagulant activity occurs in severely injured patients, which may favour alveolar fibrin deposition and is related to the development of acute respiratory failure.
Asunto(s)
Factores de Coagulación Sanguínea/metabolismo , Líquido del Lavado Bronquioalveolar/metabolismo , Síndrome de Dificultad Respiratoria/metabolismo , Heridas y Lesiones/metabolismo , Humanos , Fosfatidiletanolaminas/metabolismo , Alveolos Pulmonares/metabolismo , Síndrome de Dificultad Respiratoria/etiología , Heridas y Lesiones/complicacionesRESUMEN
Blood was obtained from 11 males participating in the Berlin marathon 1986, directly before and after the marathon, and on the three following days. Several observations were made: a) catalytic concentrations (activity) of creatine kinase (CK), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (AP) increased directly after the marathon or on the three following days; b) Cholinesterase (CHE), amylase (AML) and gamma glutamyltransferase (GGT) decreased directly after the marathon; c) the time course of AP and LDH isoenzyme activity after the race indicated an elimination from plasma to lower values than those originally observed before the run.
Asunto(s)
Enzimas/sangre , Carrera , Alanina Transaminasa/sangre , Amilasas/sangre , Aspartato Aminotransferasas/sangre , Colinesterasas/sangre , Creatina Quinasa/sangre , Hemoglobinas/análisis , Humanos , L-Lactato Deshidrogenasa/sangre , Masculino , Albúmina Sérica/análisis , gamma-Glutamiltransferasa/sangreRESUMEN
Staphylococcus aureus plays a major role as a bacterial pathogen in human medicine, causing diseases that range from superficial skin and wound to systemic nosocomial infections . The majority of S. aureus strains produces a toxin, a proteinaceous exotoxin whose hemolytic, dermonecrotic, and lethal properties have long been known (1-6). The toxin is secreted as a single- chained, nonglycosylated polypeptide with a M(r) of 3.4 x 10(4) (7, 8). The protein spontaneously binds to lipid monolayers and bilayers (9-14), producing functional transmembrane pores that have been sized to 1.5-2.0-nm diameters (15-18). The majority of pores formed at high toxin concentrations (20 mug/ml) is visible in the electron microscope as circularized rings with central pores of approximately 2 nm in diameter. The rings have been isolated, and molecular weight determinations indicate that they represent hexamers of the native toxin (7). We have proposed that transmembrane leakiness is due to embedment of these ring structures in the bilayer, with molecular flux occurring through the central channels (15, 19). Pore formation is dissectable into two steps (20, 21). Toxin monomers first bind to the bilayer without invoking bilayer leakiness . Membrane-bound monomers then laterally diffuse and associate to form non-covalently bonded oligomers that generate the pores. When toxin pores form in membranes of nucleated cells, they may elicit detrimental secondary effects by serving as nonphysiologic calcium channels, influx of this cation triggering diverse reactions, including release of potent lipid mediators originating from the arachidonate cascade (22-24). That alpha toxin represents an important factor of staphylococcal pathogenicity has been clearly established in several models of animal infections through the use of genetically engineered bacterial strains deleted of an active alpha toxin gene (25-27). Whether the toxin is pathogenetically relevant in human disease, however, is a matter of continuing debate. Doubts surrounding this issue originate from two main findings. First, whereas 60 percent hemolysis of washed rabbit erythrocytes is effected by approximately 75 ng/ml alpha toxin, approximately 100-fold concentrations are required to effect similar lysis of human cells (4-6, 13). The general consensus is that human cells display a natural resistance towards toxin attack. The reason for the wide inter-species variations in susceptibility towards alpha toxin is unknown but does not seem to be due to the presence or absence of high-affinity binding sites on the respective target cells (20, 21). Second, low-density lipoprotein (28) and neutralizing antibodies present in plasma of all healthy human individuals inactivate a substantial fraction of alpha toxin in vitro. These inactivating mechanisms presumably further raise the concentration threshold required for effective toxin attack, and it is most unlikely that such high toxin levels will ever be encountered during infections in the human organism. The aforegoing arguments rest on the validity of two general assumptions. First, the noted natural resistance of human erythrocytes to alpha toxin must be exhibited by other human cells. Second, toxin neutralization by plasma components, usually tested and quantified after their preincubation with toxin in vitro, must be similarly effective under natural conditions, and protection afforded by these components must not be restricted to specific cell species.
Asunto(s)
Toxinas Bacterianas/farmacología , Coagulación Sanguínea/efectos de los fármacos , Plaquetas/fisiología , Proteínas Hemolisinas , Adenosina Trifosfato/sangre , Toxinas Bacterianas/aislamiento & purificación , Plaquetas/efectos de los fármacos , Hemólisis/efectos de los fármacos , Humanos , L-Lactato Deshidrogenasa/sangre , Agregación Plaquetaria/efectos de los fármacos , Factor Plaquetario 4/metabolismo , Staphylococcus aureusRESUMEN
The natural antioxidant alpha-tocopherol has repeatedly been described to inhibit platelet aggregation and thromboxane formation, whereas its influence on prostaglandin H synthase in vivo and in vitro is a matter of controversy. In the present study the effects of different antioxidative compounds on ram vesicular gland microsomal prostaglandin H synthase activity were investigated in vitro: d,l-alpha-tocopherol, its carboxylic acid chromane compound (Trolox), phytol, alpha-tocopherol-acetate and two novel antioxidative isoflavanones, obtained by methylation and/or hydrogenation of naturally occurring isoflavones from fermented soybeans (6,7-dihydroxy-4'-methoxyisoflavanone and 6,7,4'-trihydroxyisoflavanone). Alpha-tocopherol, -acetate and phytol revealed no significant influence on the enzyme activity when applied in concentrations up to 1 mM. Trolox (100-1000 mu mumol/l) and the two isoflavanones (5-50 and 10-100 mumol/l) dose-dependently augmented the initial rate of oxygen consumption and the total oxygen uptake during prostaglandin H synthase incubation with arachidonic acid (AA). In parallel, these compounds increased the formation of prostaglandin E2 and F2 alpha from 14C-labelled AA, and they markedly protected the prostaglandin H synthase from rapid autodeactivation as revealed by repetitive application of AA in small doses. We suggest that these compounds serve as cosubstrates to which the oxidizing equivalents are transferred which arise during the hydroperoxidase reaction of the enzyme.
Asunto(s)
Antioxidantes/farmacología , Benzopiranos/farmacología , Cromanos/farmacología , Flavonoides/farmacología , Isoflavonas/farmacología , Vitamina E/farmacología , Animales , Ácido Araquidónico , Ácidos Araquidónicos/metabolismo , Femenino , Técnicas In Vitro , Masculino , Consumo de Oxígeno/efectos de los fármacos , Fotometría , Prostaglandina-Endoperóxido Sintasas/metabolismo , OvinosRESUMEN
The blood coagulation system is activated regularly in severe forms of shock, polytrauma, and sepsis. Arising thrombin cleaves the fibrinopeptides A and B from fibrinogen, and it generates monomers of fibrin, which are initially kept in solution by the remaining excess fibrinogen. The effects of soluble fibrin (fibrin monomer/oligomer-fibrinogen complexes) and fibrinopeptides A and B were investigated in blood-free perfused, isolated rabbit lungs. Urea Tris buffer-dissolved fibrin monomers were injected into the pulmonary artery in the presence of circulating excess fibrinogen. In doses above 5 mg, the monomers consistently provoked a sharp rise in pulmonary artery pressure, which was followed by an elevated pressure plateau. Changing to fresh perfusate devoid of soluble fibrin did not restore the pressure to baseline, and a second administration of the soluble fibrin caused a pressor response larger than the first. Only a modest increase in lung weight (less than 2 g) was observed, and lung inflation pressure was not altered. The pressor responses were accompanied by a rapid release of thromboxane A2 and a more delayed release of prostaglandin I2 into the perfusion fluid. A significant correlation between the height of the fibrin-induced pressure rise and the amount of thromboxane release was noted. Inhibition of cyclooxygenase (indomethacin) suppressed the generation of both prostanoids, whereas inhibition of thromboxane synthetase (OKY-046 and imidazole) selectively blocked the liberation of thromboxane. All three inhibitors caused an immediate decline in pulmonary artery pressure, which had been previously elevated due to administration of soluble fibrin, and markedly reduced the pressor response evoked by a subsequent fibrin application in the same lung.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Fibrina/farmacología , Circulación Pulmonar/efectos de los fármacos , Tromboxano B2/biosíntesis , Vasoconstricción , 6-Cetoprostaglandina F1 alfa/biosíntesis , Animales , Presión Sanguínea/efectos de los fármacos , Técnicas In Vitro , Perfusión , SolubilidadRESUMEN
The effects of staphylococcal alpha-toxin on arachidonic acid metabolism in rabbit polymorphonuclear leukocytes (PMNs) were investigated and compared with those of the ionophore A23187 and the chemotactic tripeptide formylmethionyl-leucyl-phenylalanine (fMLP). Sublytic amounts of alpha-toxin stimulated the release of leukotriene B4 (LTB4) in PMNs in a dose-dependent manner. The toxin was several times more potent than fMLP but was not as effective as the ionophore. Preincubation of the toxin with neutralizing antibodies abolished the effect. Extracellular calcium was strictly required for eliciting LTB4 generation. Verapamil, a calcium channel blocker, inhibited fMLP-mediated LTB4 generation but had no effect on alpha-toxin- or A23187-exposed PMNs. Agents such as trifluoperazine and N-6(aminohexyl)-5-chloro-1-naphthalene sulfonamid that interfered with calmodulin activity, however, inhibited LTB4 generation in all cases. One minute after the addition of alpha-toxin, PMNs exhibited a severalfold enhancement in passive permeability to 45Ca2+. In addition, these cells became permeable to sucrose but not to inulin or dextran. The influx pattern was consistent with the previous observation that alpha-toxin creates discrete transmembrane channels in erythrocytes with an effective internal diameter of 2 to 3 nm. The results suggest that alpha-toxin triggers the arachidonic acid pathway in PMNs by facilitating calcium influx into the cells, possibly via transmembrane toxin pores that serve as calcium gates. Generation of arachidonic acid metabolites in PMNs by sublytic amounts of alpha-toxin may represent an important cellular reaction that generally occurs during infections with Staphylococcus aureus.
Asunto(s)
Toxinas Bacterianas/farmacología , Proteínas Hemolisinas , Leucotrieno B4/biosíntesis , Neutrófilos/metabolismo , Animales , Calcimicina/farmacología , Calcio/fisiología , Bloqueadores de los Canales de Calcio/farmacología , Calmodulina/fisiología , Permeabilidad de la Membrana Celular , N-Formilmetionina Leucil-Fenilalanina/farmacología , Neutrófilos/efectos de los fármacos , Conejos , Sulfonamidas/farmacología , Trifluoperazina/farmacologíaRESUMEN
The importance of the glutathione (GSH) redox cycle and of catalase as intracellular antioxidant defense systems in cultured endothelial cells against an extracellular flux of H2O2, a critical mediator of polymorphonuclear leukocyte-induced oxidant injury of endothelial cells, was examined. The activities of different parts of the GSH redox cycle were impaired by 1,3-bis(2-chloroethyl)-1-nitrosourea, buthionine sulfoximine, diethyl maleate and 2-cyclohexene-1-one. Catalase activity was inhibited by 3-amino-1,2,4-triazole. After an impairment of the GSH redox cycle, but not of catalase, the susceptibility of pulmonary artery endothelial cells to an attack by H2O2 was dramatically increased independent of the source of extracellularly generated hydrogen peroxide (i.e., glucose oxidase or stimulated polymorphonuclear leukocytes). Exogenous catalase, d-alpha-tocopherol, and particularly Trolox, the chroman compound of tocopherol, but not phytol, the fatty acid side chain of tocopherol, provided almost complete protection of the endothelial cells against a H2O2-mediated attack. Additional fluorometric studies suggested that H2O2 is scavenged by the antioxidants before it hits the target cells.
Asunto(s)
Catalasa/metabolismo , Endotelio/metabolismo , Glutatión/metabolismo , Arteria Pulmonar/metabolismo , Amitrol (Herbicida)/farmacología , Animales , Antioxidantes/farmacología , Carmustina/farmacología , Catalasa/antagonistas & inhibidores , Células Cultivadas , Cromanos/farmacología , Endotelio/efectos de los fármacos , Glutatión Reductasa/antagonistas & inhibidores , Peróxido de Hidrógeno/farmacología , Oxidación-Reducción , Fitol/farmacología , Arteria Pulmonar/efectos de los fármacos , Porcinos , Vitamina E/farmacologíaRESUMEN
UNLABELLED: In blood-free perfused isolated rabbit lungs increased availability of free arachidonic acid (AA), whether exogenously applied or released from the endogenous membrane phospholipid pool after different stimuli, causes an acute pulmonary artery pressor response and an increase in vascular permeability. Previous experiments suggested that the vasoconstriction is caused primarily by the cyclooxygenase product thromboxane (Tx) A2, whereas an increase in the capillary filtration coefficient must be ascribed to non-cyclooxygenase products of AA. The influence of BM 13.177, a non-prostanoic antagonist of TxA2- and endoperoxide-effects in platelets, on the AA-induced vascular effects in isolated rabbit lungs was investigated. BM 13.177 dose-dependently inhibited the pressor responses evoked by repetitive direct application of AA (IC50 approximately 10(-6) M) or by repetitive stimulation of endogenous AA-release with the calcium-ionophore A 23187 (IC50 approximately 10(-7) M), with maximum reduction of the pressor responses to less than 15%. The generation of TxA2 and of prostaglandin (PG) I2 evoked by these stimuli was, however, not altered. At a concentration of 10(-5) M BM 13.177 did not influence the capillary filtration coefficient, measured during venous pressure challenge, under baseline conditions and after stimulation with AA in presence of indomethacin. CONCLUSION: BM 13.177 acts as TxA2/endoperoxide antagonist with dose-dependent inhibition of AA-induced vasoconstriction in the pulmonary vascular bed.
Asunto(s)
Ácidos Araquidónicos/farmacología , Permeabilidad Capilar/efectos de los fármacos , Fibrinolíticos/farmacología , Circulación Pulmonar/efectos de los fármacos , Sulfonamidas/farmacología , Resistencia Vascular/efectos de los fármacos , Vasoconstricción/efectos de los fármacos , 6-Cetoprostaglandina F1 alfa/análisis , Animales , Ácido Araquidónico , Presión Sanguínea/efectos de los fármacos , Calcimicina/farmacología , Técnicas In Vitro , Indometacina/farmacología , Cinética , Conejos , Tromboxano A2/análisisRESUMEN
The effects of highly purified Pseudomonas aeruginosa cytotoxin were investigated on cultured pulmonary artery endothelial cells. This toxin dose-dependently (7.5-60 micrograms/ml) and time-dependently (20-75 minutes) stimulated the release of radiolabeled arachidonic acid and metabolites and the synthesis of prostacyclin in the absence of overt cell damage (no enhanced lactate dehydrogenase [LDH] release). Preincubation of the toxin with neutralizing antibodies abolished the effect. The toxin response on endothelial cells required extracellular calcium but not magnesium and was accompanied by a calcium influx. Interference with intracellular calcium function by TMB 8 or with (calcium)-calmodulin function by trifluoperazine and W7 dose-dependently reduced the cytotoxin mediated synthesis of prostacyclin. Calcium channel blockers (nimodipine, diltiazem, verapamil, D 888), however, were ineffective in this system. Following addition of cytotoxin to endothelial cells, an increased passive permeability for small marker molecules (potassium, 45calcium, 3H-sucrose), but for large ones (3H-inulin, 3H-dextran, LDH) was noted, suggesting that cytotoxin creates discrete hydrophilic transmembrane lesions of about 0.5-1.5 nm in diameter. These data are compatible with the notion that Pseudomonas aeruginosa cytotoxin triggers the arachidonic acid pathway in cultured pulmonary artery endothelial cells by calcium influx and suggest that this calcium influx may proceed through toxin created transmembrane lesions.
Asunto(s)
Calcio/metabolismo , Citotoxinas/farmacología , Endotelio/metabolismo , Epoprostenol/biosíntesis , Pseudomonas aeruginosa , Animales , Ácido Araquidónico , Ácidos Araquidónicos/metabolismo , Calcio/farmacología , Bloqueadores de los Canales de Calcio/farmacología , Calmodulina/antagonistas & inhibidores , Membrana Celular/efectos de los fármacos , Permeabilidad de la Membrana Celular/efectos de los fármacos , Células Cultivadas , Citotoxinas/metabolismo , Endotelio/efectos de los fármacos , Modelos Biológicos , Arteria Pulmonar , Sulfonamidas/farmacología , Porcinos , Trifluoperazina/farmacologíaAsunto(s)
Electroforesis de las Proteínas Sanguíneas/veterinaria , Proteínas Sanguíneas/análisis , Ciervos , Helmintiasis Animal , Inmunoelectroforesis Bidimensional , Inmunoelectroforesis , alfa-Globulinas/análisis , Animales , beta-Globulinas/análisis , Helmintiasis/sangre , Masculino , gammaglobulinas/análisisRESUMEN
Studies in erythrocytes indicate that staphylococcal alpha-toxin generates discrete transmembrane channels with an effective diameter of 2-3 nm. In cultured, confluent, pig pulmonary arterial endothelial cells we studied the triggering of the arachidonic acid cascade and its dependence on calcium influx, possibly through toxin-created pores. In endothelial cells alpha-toxin time dependently (5-30 min) and dose dependently (0.1-8 micrograms/ml) stimulated the release of radiolabeled arachidonic acid and prostacyclin (PGI2) production in similar amounts as the calcium ionophore A23187 (10 microM). Preincubation of alpha-toxin with neutralizing antibodies abolished the effect. The toxin response was strictly dose dependent on extracellular calcium but not on magnesium. The toxin effect was accompanied by an up to 10-fold increased passive permeability of pulmonary arterial endothelial cells for 45Ca. Interference with calcium-calmodulin function (trifluoperazine, W7) dose dependently reduced production of PGI2, but blockers of physiological calcium channels (verapamil, nimodipine, nisoldipine, and diltiazem) did not. In contrast to the effect of the ionophore A23187, the toxin effect was accompanied by a release of potassium, but in neither system was there a release of lactate dehydrogenase. In addition, alpha-toxin but not ionophore-exposed endothelial cells showed an increased passive influx of small radiolabeled markers (45Ca and [3H]sucrose) but not of large markers [( 3H]inulin and [3H]dextran). These data are consistent with the concept that alpha-toxin triggers the arachidonic acid cascade in pulmonary arterial endothelial cells by calcium influx and suggest that this calcium influx may proceed through toxin-created transmembrane channels.
Asunto(s)
Toxinas Bacterianas/farmacología , Calcio/metabolismo , Epoprostenol/biosíntesis , Proteínas Hemolisinas , Músculo Liso Vascular/metabolismo , Neurotoxinas/farmacología , Arteria Pulmonar/metabolismo , 6-Cetoprostaglandina F1 alfa/biosíntesis , Animales , Calcimicina/farmacología , Bloqueadores de los Canales de Calcio/farmacología , Radioisótopos de Calcio , Endotelio/efectos de los fármacos , Endotelio/metabolismo , Cinética , Porcinos , TritioRESUMEN
A specific method for the detection of organic hydroperoxides in lung lavage fluid (lung surfactant system) and lung tissue homogenate is described. After the inactivation of endogenous GSH peroxidase and GSH reductase and preincubation with catalase, organic hydroperoxides are consumed by addition of GSH and GSH peroxidase. The increase of GSSG, compared to a blank without addition of GSH peroxidase, is measured in a second enzymatic step with GSH reductase. The recoveries of t-butyl hydroperoxide and of peroxidized, free fatty acids added to lavage fluid or to lung homogenate are higher than 85% in each case. The detection limits of this assay for organic hydroperoxides are 0.9 nmol/mg surfactant phospholipid (molar ratio of 0.00066) and 40 nmol/g wet lung weight. The assay detects organic hydroperoxides in the surfactant system of normal rabbit lungs, but not in lung tissue homogenate.
Asunto(s)
Glutatión Peroxidasa , Glutatión Reductasa , Peróxido de Hidrógeno/análisis , Pulmón/metabolismo , Surfactantes Pulmonares/metabolismo , Animales , Catalasa/metabolismo , Femenino , Técnicas In Vitro , Masculino , Conejos , Tensoactivos/farmacología , Irrigación TerapéuticaRESUMEN
Creatine kinase isoenzyme MB catalytic activities in human serum, determined by ACA ion exchange chromatography and immunoinhibition, differ significantly, the correlation coefficient being 0.88. The reasons for this variation are interference of antibodies with the creatine kinase B subunit in the immunoinhibition assay, nonreproducible elution of creatine kinase isoenzyme MB from the ion exchange resin in the ACA pack, due to varying protein concentrations in the serum samples and increasing elution of creatine kinase isoenzyme MM from the ion exchange column caused by a preceding partial inactivation of creatine kinase isoenzyme MM. Pretreatment of serum samples with a solution containing magnesium sulphate, maleate and 2-oxoglutarate (solution A) prior to determination of creatine kinase isoenzyme MB catalytic activities on the ACA significantly improves the sensitivity and specificity of the method; the correlation coefficient for the values from the ACA and immunoinhibition then becomes 0.92. Dilution of serum samples with bovine serum albumin solution is now practicable.
Asunto(s)
Creatina Quinasa/sangre , Aspartato Aminotransferasas/sangre , Proteínas Sanguíneas/análisis , Cromatografía por Intercambio Iónico/métodos , Humanos , Focalización Isoeléctrica/métodos , Isoenzimas , Miocardio/enzimologíaRESUMEN
In this communication, we propose a method for the determination in human serum of fructose 1,6-bisphosphatase based on parallel measurements of enzyme activities in presence of 1-p-bromotetramisole oxalate and adenosine 5'-monophosphate. The employment of these specific inhibitors renders the discrimination between specific and non-specific activities feasible. A regression analysis identifies fructose 1,6-bisphosphatase (EC 3.1.3.11) as the origin of the specific and alkaline phosphatase (EC 3.1.3.1) as the source of the non-specific fructose 1,6-bisphosphate dephosphorylating activities. This procedure lends itself to the diagnosis using serum samples of 'piecemeal' necrosis in liver disease.
Asunto(s)
Fructosa-Bifosfatasa/sangre , Hepatitis/diagnóstico , Adenosina Monofosfato/farmacología , Fosfatasa Alcalina/sangre , Enfermedad Crónica , Pruebas Enzimáticas Clínicas , Diagnóstico Diferencial , Femenino , Fructosa-Bifosfatasa/antagonistas & inhibidores , Humanos , Masculino , Tetramisol/análogos & derivados , Tetramisol/farmacologíaRESUMEN
The histomorphology of typical liver cell necroses are here correlated with heterotope distributions of enzymes in liver parenchyma. A variety of findings indicate a congruence between gluconeogenetic areas of the liver and the typical pattern of 'piecemeal' necrosis. We therefore propose a diagnostic index based on fructose 1,6-bisphosphatase activity and the data from the clinical laboratory. This index makes it possible to distinguish between chronic persistent and chronic aggressive hepatitis.
Asunto(s)
Fructosa-Bifosfatasa/sangre , Hepatitis/diagnóstico , Enfermedad Aguda , Enfermedad Crónica , Pruebas Enzimáticas Clínicas , Hepatitis/clasificación , Humanos , Hígado/patología , Necrosis/patologíaRESUMEN
Heparin added to citrated platelet rich plasma influences shape change and aggregation of platelets in different ways. In the presence of heparin neither ADP nor collagen induces shape change, while shape change after thrombin or arachidonic acid remains unaltered. Heparin potentiates the first aggregation step induced by ADP and epinephrine but inhibits aggregation induced by thrombin and ristocetin. The second phase of aggregation and the release reaction are not directly influenced by heparin no matter which aggregation agent is used.