RESUMEN
BACKGROUND: Participation by youth with disabilities in recreational activities has been shown to promote the development of important skills needed for transition to adulthood. The Participatory Experience Survey (PES) and the Setting Affordances Survey (SAS) were developed for use by recreational programmes serving youth with significant intellectual and developmental disabilities (SIDD) to assess participant experiences and ensure that participants are afforded optimal opportunities to develop these skills. This paper presents a feasibility evaluation to determine the appropriateness of the PES and the SAS for use in a programme evaluation context. METHOD: The PES and the SAS were used to evaluate a programme serving youth with SIDD in the greater northwest region of the United States. Three recreational activities were evaluated: an art project, trip to a zoo and a track practice. Programme volunteers used the SAS to assess opportunities and affordances offered within each activity. The PES was then given to 10 young people in each activity to capture their experiences. It was hypothesized that each setting would afford different experiences and developmental opportunities because of the differing nature of the activities. RESULTS: The PES and SAS were found to be feasible for conducting a programme evaluation. All three settings offered varying types of experiences and affordances. Notably, as measured by the SAS, opportunity for skill development was greater in more structured activities; the zoo had the fewest opportunities for skill development and the art project had the most skill development opportunities. Youth answered 'no' most often to 'asking for help' and 'helping a kid', suggesting changes to offer more opportunities to develop these skills would be beneficial in all three activities. CONCLUSION: These new instruments offer programmes a means to more fully include young people with disabilities during programme evaluations, leading to better-structured, more supportive programmes.
Asunto(s)
Discapacidades del Desarrollo/rehabilitación , Personas con Discapacidad , Discapacidad Intelectual/rehabilitación , Cooperación del Paciente/estadística & datos numéricos , Terapia Recreativa , Adolescente , Investigación Participativa Basada en la Comunidad , Discapacidades del Desarrollo/psicología , Personas con Discapacidad/psicología , Personas con Discapacidad/rehabilitación , Estudios de Factibilidad , Femenino , Encuestas de Atención de la Salud , Humanos , Discapacidad Intelectual/psicología , Masculino , Noroeste de Estados Unidos , Evaluación del Resultado de la Atención al Paciente , Evaluación de Programas y Proyectos de Salud , Adulto JovenRESUMEN
Recently, voltammetry with carbon fibre electrodes (CFE) has been implemented for real time measurement of nitrogen monoxide (NO) indicating that it is oxidised at the potential value of nitrites, approximately +700 mV. In contrast, here we show that modified CFE can monitor NO at oxidation potentials different than that of nitrites, i.e. +550 mV. Indeed, at +550 mV a significant increase of amperometric current levels was obtained when NO but not nitrites, were added to a phosphate buffer saline solution (PBS). Differential pulse voltammetry (DPV) supports these findings as two oxidation peaks were obtained when examining air preserved NO; peak 1 at +550 mV and peak 2 at +700 mV, respectively. In contrast, only peak 2 was monitored when nitrites or a solution of NO oxidised in air was added to PBS. Biological support to these in vitro data comes from the observation that the relaxation of an adrenaline-contracted aortic ring produced via addition of NO is concomitant with peak 1 at +550 mV. The relaxation is almost completed before the appearance of peak 2 at +700 mV. Furthermore, in vivo experiments performed in the striatum of rats show that the amperometric signal monitored at +550 mV is responsive to glutamatergic stimulation or inhibition of NO synthase.
Asunto(s)
Carbono , Electrofisiología/métodos , Potenciales de la Membrana/fisiología , Microelectrodos/tendencias , Neuroquímica/métodos , Óxido Nítrico/análisis , Nitritos/análisis , 2-Amino-5-fosfonovalerato/farmacología , Animales , Aorta/efectos de los fármacos , Aorta/metabolismo , Carbono/normas , Fibra de Carbono , Electrofisiología/instrumentación , Inhibidores Enzimáticos/farmacología , Epinefrina/farmacología , Agonistas de Aminoácidos Excitadores/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Masculino , Microelectrodos/normas , Contracción Muscular/efectos de los fármacos , Contracción Muscular/fisiología , Músculo Liso Vascular/efectos de los fármacos , Músculo Liso Vascular/metabolismo , N-Metilaspartato/farmacología , NG-Nitroarginina Metil Éster/farmacología , Neostriado/efectos de los fármacos , Neostriado/metabolismo , Neuroquímica/instrumentación , Ratas , Ratas WistarRESUMEN
Extracellular glutamate levels were measured by microdialysis in the prefrontal cortex (PFC) of anaesthetised rats in response to a short, experimenter-provoked mechanical movement of the animal head. Movement caused significant, nerve impulse-independent elevations of glutamate levels (maximum increase, 300+/-30% of baseline). This study reveals a possible artifact in the measurement of extracellular glutamate concentrations by microdialysis and suggests that, in awake animals, treatments associated with stimulation of motor activity can cause non-specific efflux of glutamate in the PFC.
Asunto(s)
Artefactos , Ácido Glutámico/metabolismo , Microdiálisis/normas , Movimiento/fisiología , Corteza Prefrontal/metabolismo , Anestesia , Animales , Nivel de Alerta/fisiología , Espacio Extracelular/metabolismo , Masculino , Actividad Motora/fisiología , Ratas , Ratas Sprague-DawleyRESUMEN
It has been hypothesised that rat lines genetically selected for their alcohol preference consume large amounts of ethanol because they have a low 5-HT content. Since brain tryptophan (TRP) availability controls the rate at which neurons synthesise and release serotonin (5-HT), we assessed whether the administration of a TRP-supplemented or TRP-free diet for 3 consecutive days influenced alcohol intake in alcohol-preferring and non-preferring sP and sNP rats, respectively. In the same animals extracellular 5-HT concentration was monitored by microdialysis in the frontal cortex. A TRP-free diet progressively and markedly decreased cortical extracellular 5-HT in sP and sNP rats during the treatment period with respect to a balanced diet. However, the TRP-free diet failed to modify alcohol consumption and preference in sP and sNP rats. The TRP-supplemented diet also failed to alter the intake of alcohol in either group of rats. Therefore, these results do not support a specific role of 5-HT transmission in ethanol intake and preference in sP and sNP rats.
Asunto(s)
Depresores del Sistema Nervioso Central/metabolismo , Etanol/metabolismo , Preferencias Alimentarias/fisiología , Lóbulo Frontal/metabolismo , Neurotransmisores/metabolismo , Serotonina/metabolismo , Triptófano/metabolismo , Animales , Conducta Consumatoria , Masculino , Microdiálisis , Neurotransmisores/líquido cefalorraquídeo , Ratas , Ratas Endogámicas , Serotonina/líquido cefalorraquídeo , Triptófano/administración & dosificaciónRESUMEN
The effect of NMDA on the motility of the rat portal vein was studied in an isolated preparation. NMDA induced a concentration-dependent (10(-7) - 10(-4) M) increase of the contraction frequency (maximum increase, 148+/-6% of control at NMDA 10(-4) M). The NMDA-induced excitatory response was prevented by the competitive NMDA receptor antagonists (+/-)-2-Amino-5-phosphonopentanoic acid (AP-5, 5x10(-4) M) or (RS)-3-(2-carboxypiperazine-4-yl) propyl-1-phosphonic acid (CPP, 10(-4) M). Tetrodotoxin (TTX, 10(-6) M) or atropine (10(-4) M) abolished the NMDA-induced increase of the portal vein motility and reversed the excitatory effect to a concentration-dependent inhibition (maximum inhibition, 52+/-8 and 29+/-7% of controls, respectively, at NMDA 10(-3) M). Removal of the endothelium abolished the NMDA-induced inhibitory response. Sodium nitroprusside concentration-dependently (10(-7) - 10(-5) M) inhibited the portal vein motility, while L-N(G)-nitro-arginine methyl ester (L-NAME, 10(-4) M) reversed the inhibitory effect of NMDA (in the presence of TTX), restoring the portal vein spontaneous activity to control values. These results show that NMDA modulates the portal vein motility in a biphasic manner: via indirect activation, through prejunctional NMDA receptors presumably located on intrinsic excitatory neuronal afferences, or via direct inhibition, through endothelial NMDA receptors activating the nitric oxide pathway. Overall these findings support the hypothesis of the existence of a peripheral glutamatergic innervation modulating the contractile activity of the rat portal vein. British Journal of Pharmacology (2000) 129, 156 - 162
Asunto(s)
Agonistas de Aminoácidos Excitadores/farmacología , Músculo Liso Vascular/efectos de los fármacos , N-Metilaspartato/farmacología , Vena Porta/efectos de los fármacos , 2-Amino-5-fosfonovalerato/farmacología , Animales , Atropina/farmacología , Inhibidores Enzimáticos/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Técnicas In Vitro , Masculino , Antagonistas Muscarínicos/farmacología , Contracción Muscular/efectos de los fármacos , N-Metilaspartato/antagonistas & inhibidores , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico Sintasa/antagonistas & inhibidores , Óxido Nítrico Sintasa de Tipo III , Nitroprusiato/farmacología , Piperazinas/farmacología , Ratas , Ratas Wistar , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Tetrodotoxina/farmacología , Vasodilatadores/farmacologíaRESUMEN
Ethanol withdrawal is a physiopathological state associated with increased number and function of N-methyl-D-aspartate glutamate receptors. We assessed the effect of N-methyl-D-aspartate receptor stimulation on the extracellular levels of glutamate in vivo by the focal application of N-methyl-D-aspartate in the striatum of dependent rats following withdrawal from chronic treatment with ethanol. In control, chronic sucrose-treated rats, 800 microM N-methyl-D-aspartate increased glutamate levels to 268% of baseline values. In ethanol-withdrawn animals, 12 h after interruption of the chronic treatment, the application of N-methyl-D-aspartate increased glutamate levels to 598% of baseline values. In ethanol-intoxicated rats N-methyl-D-aspartate was ineffective. Concentration-response curves showed that in ethanol withdrawn animals N-methyl-D-aspartate was five-fold more potent than in controls. In withdrawn animals, the non-competitive N-methyl-D-aspartate receptor antagonist dizocilpine (1.0 mg/kg i.p.) or ethanol (5 g/kg i.g.) markedly reduced the N-methyl-D-aspartate-induced increase in glutamate levels. These results are consistent with the up-regulation of N-methyl-D-aspartate receptors by chronic ethanol and add biochemical evidence for the presence of N-methyl-D-aspartate receptors facilitating glutamate release through a positive feedback mechanism. The glutamate-induced, N-methyl-D-aspartate receptor-mediated elevations of extracellular glutamate may constitute a neurochemical substrate for the neuropathological alterations associated with alcoholism.
Asunto(s)
Cuerpo Estriado/efectos de los fármacos , Etanol/efectos adversos , Agonistas de Aminoácidos Excitadores/farmacología , Ácido Glutámico/fisiología , N-Metilaspartato/farmacología , Receptores de N-Metil-D-Aspartato/efectos de los fármacos , Síndrome de Abstinencia a Sustancias/metabolismo , Animales , Cuerpo Estriado/fisiopatología , Maleato de Dizocilpina/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Espacio Extracelular/metabolismo , Retroalimentación , Ratas , Ratas Sprague-Dawley , Receptores de N-Metil-D-Aspartato/biosíntesis , Receptores de N-Metil-D-Aspartato/fisiología , Regulación hacia Arriba/efectos de los fármacosRESUMEN
Withdrawal from chronic ethanol intoxication is associated with a reduction of dopamine neurotransmission. However, the mechanisms of dopamine depletion, a putative neurochemical correlate of the dysphoric symptomatology, are not yet understood. To assess the role of L-type calcium channels in the inhibition of the dopaminergic system in the withdrawal state, the effects of the dihydropyridine calcium channel antagonist nimodipine on the extracellular levels of dopamine were studied in the nucleus accumbens shell of awake rats 10 h after withdrawal from chronic ethanol intoxication. In control, chronic sucrose-withdrawn rats, nimodipine did not change extracellular dopamine levels. However, in ethanol-withdrawn rats nimodipine (5 or 10 mg/kg s.c.) increased extracellular dopamine to 136 +/- 16 and 305 +/- 19% of pre-administration values, respectively, the latter dose elevating levels above those of controls. The elevations of extracellular DA by nimodipine (10 mg/kg) were associated with a significant reduction (-17%) of the overall behavioural score of the withdrawal symptomatology, as evaluated for 11 behavioural items. Significant reductions of the score for convulsions (-47%) and, to a lesser extent, for catatonia (-30%) and tremors (-15%) contributed to the overall effect. It is suggested that overactivity of L-type calcium channels is involved in the mechanisms of dopamine depletion as well as in certain behavioural/neurological signs associated with ethanol withdrawal. By restoring depleted dopamine levels, dihydropyridines might ameliorate the dysphoric symptoms of ethanol abstinence.
Asunto(s)
Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio Tipo L/metabolismo , Dopamina/metabolismo , Etanol/efectos adversos , Nimodipina/farmacología , Núcleo Accumbens/efectos de los fármacos , Síndrome de Abstinencia a Sustancias/metabolismo , Alcoholismo/metabolismo , Análisis de Varianza , Animales , Masculino , Núcleo Accumbens/metabolismo , Ratas , Ratas Sprague-DawleyRESUMEN
Long-term ethanol consumption in humans and laboratory animals is associated with morphological and functional alterations of brain structures involved in cognitive processes. In the present experiments, we assessed whether voluntary long-term consumption of ethanol by alcohol-preferring (sP) rats under free choice condition with water (also) caused alterations in memory performance and hippocampal acetylcholine (ACh) release in vivo. A group of sP rats were offered a 10% v/v ethanol solution in a free choice with water for 36 weeks; controls had only tap water available. After withdrawal of ethanol, rats were tested in one trial passive avoidance test and thereafter were trained in a food-reinforced radial arm maze task for 12 days. One day after the last session in the radial-arm maze, rats were implanted with a microdialysis probe in the dorsal hippocampus and dialysate concentrations of ACh were measured. No significant differences were observed between sP drinking and control rats in retention latencies in the passive avoidance test, in radial arm-maze performance or in basal levels of hippocampal ACh release. These results show that long-term ethanol consumption by sP rats is not associated with cognitive impairments or with alterations in the hippocampal cholinergic function. To the extent that chronic ethanol intoxication can be considered a causal factor in the development of memory and neurochemical alterations, these results suggest that sP rats self-regulate ethanol consumption so as to avoid intoxication. These findings may challenge the notion that sP rat lines can be considered a valid model of human alcoholism.
Asunto(s)
Acetilcolina/metabolismo , Consumo de Bebidas Alcohólicas/metabolismo , Consumo de Bebidas Alcohólicas/psicología , Reacción de Prevención/fisiología , Hipocampo/metabolismo , Aprendizaje por Laberinto/fisiología , Percepción Espacial/fisiología , Consumo de Bebidas Alcohólicas/genética , Animales , Peso Corporal/fisiología , Masculino , Microdiálisis , RatasRESUMEN
In this review first we evaluate evidence on the role of the neurobiological alterations induced by chronic ethanol consumption in the development of ethanol tolerance, dependence and withdrawal. Secondly, we describe the neuropathological consequences of chronic ethanol on cognitive functions and on brain structures. Chronic alcohol consumption can induce alterations in the function and morphology of most if not all brain systems and structures. While tolerance mechanisms are unlikely to contribute to the neuroadaptive changes associated with ethanol dependence, it is otherwise clear that repeated high, intoxicating doses of ethanol trigger those neuroadaptive processes that lead to dependence and contribute to the manifestation of the abstinence syndrome upon withdrawal. An unbalance between inhibitory and excitatory neurotransmission is the most prominent neuroadaptive process induced by chronic ethanol consumption. Due to the diffuse glutamatergic innervation to all brain structures, the neuroadaptive alterations in excitatory neurotransmission can affect the function of most if not all of neurotransmitter systems. The expression of the withdrawal syndrome is the major causal factor for the onset and development of the neuropathological alterations. This suggests a link between the neuroadaptive mechanisms underlying the development of ethanol dependence and those underlying the functional and structural alterations induced by chronic ethanol. In animals and humans, specific alterations occur in the function and morphology of the diencephalon, medial temporal lobe structures, basal forebrain, frontal cortex and cerebellum, while other subcortical structures, such as the caudate nucleus, seem to be relatively spared. The neuropathological alterations in the function of mesencephalic and cortical structures are correlated with impairments in cognitive processes. In the brain of alcoholics, the prefrontal cortex and its subterritories seem particularly vulnerable to chronic ethanol, whether Korsakoff's syndrome is present or not. Due to the role of these cortical structures in cognitive functions and in the control of motivated behavior, functional alterations in this brain area may play an important role in the onset and development of alcoholism.
Asunto(s)
Trastornos Relacionados con Alcohol/fisiopatología , Alcoholismo/complicaciones , Etanol/efectos adversos , Degeneración Nerviosa/inducido químicamente , Enfermedades del Sistema Nervioso/inducido químicamente , Acetilcolina/fisiología , Adaptación Fisiológica , Trastorno Amnésico Alcohólico/etiología , Consumo de Bebidas Alcohólicas/efectos adversos , Animales , Apoptosis , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Encéfalo/patología , Condicionamiento Clásico , Depresión/inducido químicamente , Depresión/fisiopatología , Modelos Animales de Enfermedad , Dopamina/fisiología , Tolerancia a Medicamentos , Etanol/toxicidad , Humanos , Degeneración Nerviosa/fisiopatología , Óxido Nítrico/fisiología , Estrés Oxidativo , Receptores de N-Metil-D-Aspartato/fisiología , Síndrome de Abstinencia a Sustancias/etiología , Deficiencia de Tiamina/inducido químicamente , Deficiencia de Tiamina/complicaciones , Ácido gamma-Aminobutírico/fisiologíaRESUMEN
Electrical stimulation of the medial prefrontal cortex caused glutamate release in the ventral tegmental area (VTA) of freely moving animals. Cathodal stimulation was given through monopolar electrodes in 0.1-ms pulses at an intensity of 300 microA and frequencies of 4-120 Hz. Glutamate was measured in 10-min perfusate samples by HPLC coupled with fluorescence detection following precolumn derivatization with o-phthaldialdehyde/beta-mercaptoethanol. The stimulation-induced glutamate release was frequency dependent and was blocked by the infusion of the sodium channel blocker tetrodotoxin (10 microM) through the dialysis probe. The stimulation also induced bilateral Fos-like immunoreactivity in ventral tegmental neurons, with a significantly greater number of Fos-positive cells on the stimulated side. These findings add to a growing body of evidence suggesting that the medial prefrontal cortex regulates dopamine release in the nucleus accumbens via its projection to dopamine cell bodies in the VTA.
Asunto(s)
Espacio Extracelular/metabolismo , Ácido Glutámico/metabolismo , Corteza Prefrontal/fisiología , Proteínas Proto-Oncogénicas c-fos/metabolismo , Estilbamidinas , Tegmento Mesencefálico/metabolismo , Animales , Estimulación Eléctrica , Colorantes Fluorescentes , Ratas , Tegmento Mesencefálico/efectos de los fármacos , Tetrodotoxina/farmacologíaRESUMEN
A set of substituted benzamides, characterized by the presence of a bulky quinolizidine moiety, were subjected to binding assays for 5-HT3 and D2 receptors on membranes obtained from the bovine area postrema ([3H]-GR65630) and the rat striatum ([3H]-spiperone) respectively. These benzamides resulted unsuitable for the recognition of D2 receptors, while a few of them, devoid of 5-HT4 receptor activity, had consistent affinity for central 5-HT3 receptors, inhibiting also potently the ethanol-induced dopamine efflux from the mesolimbic dopamine terminal region. However they failed in attenuating voluntary alcohol consumption in rats, as observed with several other chemically unrelated 5-HT3 antagonists. Thus the 5-HT3-mediated inhibition of alcohol-induced striatal release of dopamine by substituted benzamides is not a requisite for affecting ethanol intake.
Asunto(s)
Consumo de Bebidas Alcohólicas , Benzamidas/farmacología , Dopamina/metabolismo , Etanol/antagonistas & inhibidores , Receptores de Serotonina/metabolismo , Antagonistas de la Serotonina/farmacología , Animales , Benzamidas/química , Benzamidas/metabolismo , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Bovinos , Etanol/farmacología , Masculino , Estructura Molecular , Núcleo Accumbens/efectos de los fármacos , Núcleo Accumbens/metabolismo , Ratas , Receptores de Dopamina D2/efectos de los fármacos , Receptores de Serotonina 5-HT3 , Antagonistas de la Serotonina/química , Antagonistas de la Serotonina/metabolismoRESUMEN
Extracellular glutamate was measured by microdialysis in the striatum of ethanol-dependent, freely behaving rats following withdrawal from chronic ethanol treatment. Within 12 h from withdrawal, extracellular glutamate rose to 255% of that in control, chronic sucrose-treated rats. Glutamate output remained elevated for the subsequent 12 h and returned to control levels within 36 h from the interruption of the treatment. The changes in glutamate were time-locked to the overt physical signs of withdrawal. In 12-h ethanol-withdrawn rats an ethanol challenge suppressed the withdrawal signs and reduced the extracellular glutamate. The NMDA receptor antagonist, dizocilpine, reduced both the physical signs of withdrawal and glutamate output. In contrast, diazepam reduced the withdrawal signs but failed to change the glutamate levels. These findings suggest that the increased extraneuronal glutamate reflects overactivity of excitatory neurotransmission during withdrawal. Furthermore, they provide a biochemical rationale for the use of NMDA receptor antagonists and ethanol itself in the treatment of ethanol withdrawal syndrome.
Asunto(s)
Cuerpo Estriado/metabolismo , Etanol/farmacología , Ácido Glutámico/metabolismo , Síndrome de Abstinencia a Sustancias , Animales , Diazepam/farmacología , Maleato de Dizocilpina/farmacología , Masculino , Microdiálisis , Ratas , Ratas Sprague-Dawley , Factores de TiempoRESUMEN
Dizocilpine administration enhances dopamine metabolism in the rat striatum, nucleus accumbens, olfactory tubercle, and prefrontal cortex. Concomitant with increased metabolism is enhanced tyrosine hydroxylase and aromatic L-amino acid decarboxylase activities in the striatum and increased mRNA for the two enzymes in the midbrain. Activation of dopaminergic neurons may, in part, explain increased locomotor activity in normal animals and the ability of dizocilpine to potentiate the antiparkinsonian action of L-3,4-dihydroxyphenylalanine in an animal model.
Asunto(s)
Descarboxilasas de Aminoácido-L-Aromático/efectos de los fármacos , Cuerpo Estriado/enzimología , Maleato de Dizocilpina/farmacología , Tirosina 3-Monooxigenasa/efectos de los fármacos , Análisis de Varianza , Animales , Descarboxilasas de Aminoácido-L-Aromático/metabolismo , Northern Blotting , Encéfalo/enzimología , Masculino , Ratas , Ratas Sprague-Dawley , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
Exposure of rate to the behavioral despair test (an animal model of depression) for 40 min resulted in a long-lasting depletion of mesolimbic dopamine output to about 40% of baseline values. The decrease in extracellular dopamine was partially prevented by chronic pretreatment with imipramine (20 mg/kg per day i.p. for 21 days). The results suggest that a fall in mesolimbic dopamine output may be associated with depressive states and indicate that changes in the functional status of the dopamine system contribute to the mechanism of action of imipramine.
Asunto(s)
Cuerpo Estriado/efectos de los fármacos , Depresión/tratamiento farmacológico , Dopamina/metabolismo , Imipramina/uso terapéutico , Ácido 3,4-Dihidroxifenilacético/metabolismo , Animales , Cuerpo Estriado/metabolismo , Depresión/metabolismo , Modelos Animales de Enfermedad , Ácido Homovanílico/metabolismo , Masculino , Ratas , Ratas Sprague-Dawley , Factores de TiempoRESUMEN
Activity of the mesolimbic dopaminergic system was investigated in rats withdrawn from chronic ethanol administration by single-cell extracellular recordings from dopaminergic neurons of the ventrotegmental area, coupled with antidromic identification from the nucleus accumbens, and by microdialysis-technique experiments in the nucleus accumbens. Spontaneous firing rates, spikes per burst, and absolute burst firing but not the number of spontaneously active neurons were found drastically reduced; whereas absolute and relative refractory periods increased in rats withdrawn from chronic ethanol treatment as compared with chronic saline-treated controls. Consistently, dopamine outflow in the nucleus accumbens and its acid metabolites were reduced after abruptly stopping chronic ethanol administration. All these changes, as well as ethanol-withdrawal behavioral signs, were reversed by ethanol administration. This reversal suggests that the abrupt cessation of chronic ethanol administration plays a causal role in the reduction of mesolimbic dopaminergic activity seen in the ethanol-withdrawal syndrome. Results indicate that during the ethanol-withdrawal syndrome the mesolimbic dopaminergic system is tonically reduced in activity, as indexed by electrophysiological and biochemical criteria. Considering the role of the mesolimbic dopaminergic system in the reinforcing properties of ethanol, the depressed activity of this system during the ethanol-withdrawal syndrome may be relevant to the dysphoric state associated with ethanol withdrawal in humans.
Asunto(s)
Delirio por Abstinencia Alcohólica/fisiopatología , Intoxicación Alcohólica/fisiopatología , Dopamina/metabolismo , Etanol/toxicidad , Neuronas/fisiología , Núcleo Accumbens/fisiopatología , Tegmento Mesencefálico/fisiopatología , Ácido 3,4-Dihidroxifenilacético/aislamiento & purificación , Ácido 3,4-Dihidroxifenilacético/metabolismo , Potenciales de Acción/efectos de los fármacos , Delirio por Abstinencia Alcohólica/metabolismo , Análisis de Varianza , Animales , Dopamina/aislamiento & purificación , Ácido Homovanílico/aislamiento & purificación , Ácido Homovanílico/metabolismo , Masculino , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Núcleo Accumbens/efectos de los fármacos , Núcleo Accumbens/metabolismo , Ratas , Ratas Sprague-Dawley , Tegmento Mesencefálico/efectos de los fármacos , Tegmento Mesencefálico/metabolismo , Factores de TiempoRESUMEN
The administration of ethanol (2 g/kg, i.p.) or of the non-competitive antagonist(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cycloepten-5,1 0-imine maleate (MK-801; 1 mg/kg, i.p.) induced a decrease in the extracellular concentrations of glutamate, as studied by microdialysis in the striatum of awake rats. Moreover, ethanol and MK-801 completely prevented the increase in extraneuronal glutamate concentration induced by the focal application of N-methyl-D-aspartate (NMDA). The present results suggest that ethanol suppresses glutamate release through an inhibition of NMDA glutamate receptors in the rat striatum.
Asunto(s)
Cuerpo Estriado/efectos de los fármacos , Etanol/farmacología , Glutamatos/metabolismo , N-Metilaspartato/antagonistas & inhibidores , Receptores de N-Metil-D-Aspartato/efectos de los fármacos , Animales , Cuerpo Estriado/metabolismo , Maleato de Dizocilpina/farmacología , Ácido Glutámico , Masculino , N-Metilaspartato/farmacología , Ratas , Ratas Sprague-Dawley , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Receptores de N-Metil-D-Aspartato/fisiologíaRESUMEN
The changes in extracellular concentrations of noradrenaline (NA) in the prefrontal cortex of morphine-dependent rats were studied by microdialysis following an acute morphine challenge and during naloxone-precipitated withdrawal. Animals were implanted with morphine- or placebo-containing pellets for 5 days. In control rats a challenge dose of morphine (5 mg/kg s.c.) induced a maximum decrease in NA output of about 45% of pre-drug levels. In contrast, morphine challenge had no effect on extraneuronal NA concentrations in morphine-implanted animals. In control animals, naloxone (2 mg/kg i.p.) produced no behavioral effect nor changed NA levels. However, in morphine-dependent animals naloxone suddenly increased extraneuronal NA by 175% of baseline dialysate levels in the first sample after the injection and precipitated a morphine-withdrawal symptomatology that paralleled the changes in NA output. Thus, chronic morphine treatment in rats results in the development of tolerance to the acute inhibitory effect of morphine on extraneuronal NA and is associated with a stimulation of prefrontocortical NA output during naloxone-precipitated withdrawal.
Asunto(s)
Dependencia de Morfina/metabolismo , Norepinefrina/metabolismo , Corteza Prefrontal/metabolismo , Síndrome de Abstinencia a Sustancias/metabolismo , Animales , Diálisis , Tolerancia a Medicamentos , Locus Coeruleus/efectos de los fármacos , Locus Coeruleus/metabolismo , Masculino , Naloxona/farmacología , Corteza Prefrontal/efectos de los fármacos , Ratas , Ratas Sprague-DawleyAsunto(s)
Industrias , Nicotiana , Nicotina , Plantas Tóxicas , Trastornos Relacionados con Sustancias , Humanos , ItaliaRESUMEN
The effect of ethanol challenge on the extracellular concentrations of dopamine, 3,4-dihydroxy-phenylacetic acid and homovanillic acid was studied in the ventral striatum of rats repeatedly treated with ethanol. Ethanol-treated animals (1 g/kg i.p. twice a day for 12 days) developed marked tolerance to the behavioral signs of ethanol intoxication when challenged with ethanol (2 g/kg i.p.). However, in ethanol-treated animals the increased output of dopamine and metabolites after ethanol challenge (1 or 2 g/kg i.p.) was not statistically different from that observed in saline-treated rats. These results indicate that tolerance does not develop to the ethanol-induced stimulation of dopamine release and support the hypothesis that activation of the mesolimbic dopamine system contributes to the reinforcing properties of ethanol.
Asunto(s)
Cuerpo Estriado/metabolismo , Dopamina/metabolismo , Etanol/farmacología , Ácido 3,4-Dihidroxifenilacético/metabolismo , Animales , Conducta Animal/efectos de los fármacos , Cuerpo Estriado/efectos de los fármacos , Diálisis , Tolerancia a Medicamentos , Ácido Homovanílico/metabolismo , Masculino , Terminaciones Nerviosas/efectos de los fármacos , Terminaciones Nerviosas/metabolismo , Ratas , Ratas Sprague-DawleyRESUMEN
Ethanol (EtOH) administration is considered to elicit its reinforcing properties by stimulating dopaminergic (DA) transmission in the mesolimbic system. Accordingly, (EtOH) activates dopamine neuronal firing in the Ventro-Tegmental Area (VTA) and DA output in the nucleus accumbens. Concomitantly, EtOH reduces the firing rate of Pars Reticulata (PR) neurons which are thought to exert an inhibitory control over DA neurons. Further, chronic ingestion of EtOH produces tolerance to its sedative effects as to the depressant effect on PR neurons but no tolerance to the DA stimulating action. Moreover the NMDA antagonist MK-801, but not SL-820715, stimulates DA firing, suggesting that this effect is not a general characteristic of NMDA receptor antagonists and questioning the possibility that NMDA-receptor blockade may underlie EtOh-induced activation of DA-ergic transmission. The results indicate that activation of the mesolimbic DA tract is essential in the rewarding properties of EtOH and that neither GABA-ergic inhibition nor NMDA-receptor blockade by EtOH, are causally linked to the EtOH-induced activation of DA-ergic transmission.