[Interleukin-17-mediated inflammation promotes nonalcoholic fatty liver disease in mice with regulation of M1-type macrophage polarization].

Objective: To study the mechanism of interleukin (IL)-17 in mice with non-alcoholic fatty liver disease for promoting M1-type macrophage polarization to exacerbate liver inflammation, and to provide references for the mechanism of NAFLD occurrence and development. Methods: A mouse model of NAFLD was constructed by high-fat diet. Mice were divided into control group, model group, IL-17 group, and anti IL-17 group. Histopathological changes of the liver were observed by HE staining. The serum levels of ALT and AST in peripheral blood of mice was detected by chemical colorimetry. Macrophages labeled with F4/80-PE, CD11C-FITC was designated as M1-type macrophages, those labeled with F4/80-PE, and CD206-APC was designated as M2-type macrophages. The proportion of M1 and M2 macrophages infiltrated into the liver tissues of mice were measured by flow cytometry. CD168 expression level of liver tissues was detected using immunohistochemistry. Protein and mRNA levels of the marker molecules (iNOS, TNF-alpha and IL-6) of M1 macrophages were detected using ELISA and RT-Q PCR. Western blot was used to detect the protein expression of JAK-STAT signal pathway and the expression level of MCP-1. Data were analyzed using one-way ANOVA and t-test. Results: High-fat diet NAFLD mice model was successfully constructed. IL-17 had increased the proportion of M1 macrophages in mice liver tissues and decreased the proportion of M2 macrophages (P < 0.05). The proportion of M1 and M2 macrophages in the liver tissues of normal mice was 7.9% ± 1.1% and 19.2% ± 1.8%. The proportion of M1 and M2 macrophages in the model group was 17.3% ± 2.5% and 15.0% ± 2.1. The proportion of M1 macrophages (33.8% ± 4.2%) in IL-17 group was higher than model group, while the proportion of M2 macrophages (7.8% + 1.0%) in IL-17 group was lower than model group. Protein and mRNA marker levels of M1 macrophage (iNOS, IL-12, TNFα and IL-6) in liver tissues were significantly higher than model group, control group, and anti-IL-17 group (P < 0.05). The expression levels of JAK1, STAT1, MCP-1, and CD168 in mice liver tissues of IL-17 group had increased (P < 0.05). The levels of aspartate and alanine aminotransferases in peripheral blood of mice in IL-17 group were significantly higher than other three groups (P < 0.05). Conclusion: IL-17 can promote M1-type macrophage polarization, and exacerbates the liver inflammatory response to accelerate the progression of NAFLD in mice.

Effects of reflection-induced retardance on the immunity of bulk optic-material current sensors.

Reflection-induced phase retardance inside bulk optic-material current sensors makes the state of polarization of the light change, which reduces the immunity of the sensors against electromagnetic interference. These effects are analyzed theoretically for the first time, to our knowledge. A comparison between the theoretical analysis and the experimental results are given.

Polarization-preserving totally reflecting prisms with a single medium layer.

When reflectors with layers are used at non-normal incidence, the two planes of polarization generally have different phase shifts. The difference in the shifts is known as phase retardance. For prisms with a single layer, this retardance depends on four factors: prism index, layer index, the ratio of the optical thickness of the layer to the wavelength, and angle of incidence. When the retardance is kept at zero and the reflectance for both p and s components of a polarized light are controlled at almost 100% after reflection, a polarization-preserving total reflection is realized. Polarization-preserving totally reflecting prisms (PPTRP's) have many applications in scientific research and optical engineering. Designers of the PPTRP's need references concerning the phase properties of the PPTRP's. However, few papers can be found in which the effects of the four factors on the retardance of the PPTRP's were investigated and compared thoroughly, although the theory concerned has been known and the influences from some (not all) factors have been reported. Therefore it is still necessary to study the behavior of the PPTRP's from all aspects. The effects of all four factors on the retardance are analyzed and compared from all aspects. A general method of designing PPTRP's is proposed. As an example, a special PPTRP is designed and both theoretical and experiment results are given.