Effect of Light Intensity under Different Photoperiods on Expression Level of Carbonic Anhydrase Genes of the α- and ß-Families in Arabidopsis thaliana Leaves.

Changes in expression levels of genes encoding carbonic anhydrases α-CA1, α-CA2, α-CA4, ß-CA1, ß-CA2, ß-CA3, ß-CA4, ß-CA5, and ß-CA6 in Arabidopsis thaliana leaves after light increase from 80 to 400 µmol PAR quanta·m-2·s-1 were investigated under short day (8 h) and long day (16 h) photoperiods. The expression of two forms of the gene, At3g01500.2 and At3g01500.3, encoding the most abundant carbonic anhydrase of leaves, ß-CA1, situated in chloroplast stroma, was found. The content of At3g01500.3 transcripts was higher by approximately an order of magnitude compared to the content of At3g01500.2 transcripts. When plants were adapted to high light intensity under short day photoperiod, the expression level of both forms increased, whereas under long day photoperiod, the content of At3g01500.3 transcripts increased, and the content of transcripts of At3g01500.2 decreased. The expression levels of the At3g01500.3 gene and of genes encoding chloroplast carbonic anhydrases α-CA1, α-CA4, α-CA2 and cytoplasmic carbonic anhydrase ß-CA2 increased significantly in response to increase in light intensity under short day, and these of the first three genes increased under long day as well. The expression level of the gene encoding α-CA2 under long day photoperiod as well as of genes of chloroplast ß-CA5 and ß-CA4 from plasma membranes and mitochondrial ß-CA6 under both photoperiods depended insignificantly on light intensity. Hypotheses about the roles in higher plant metabolism of the studied carbonic anhydrases are discussed considering the effects of light intensity on expression levels of the correspondent genes.

Participation of Two Carbonic Anhydrases of the Alpha Family in Photosynthetic Reactions in Arabidopsis thaliana.

The expression of genes of two carbonic anhydrases (CA) belonging to the α-family, α-CA2 and α-CA4 (according to the nomenclature in N. Fabre et al. (2007) Plant Cell Environ., 30, 617-629), was studied in arabidopsis (Arabidopsis thaliana, var. Columbia) leaves. The expression of the At2g28210 gene coding α-CA2 decreased under increase in plant illumination, while the expression of the At4g20990 gene coding α-CA4 increased. Under conditions close to optimal for photosynthesis, in plants with gene At2g28210 knockout, the effective quantum yield of photosystem 2 and the light-induced accumulation of hydrogen peroxide in leaves were lower than in wild type plants, while the coefficient of non-photochemical quenching of leaf chlorophyll a fluorescence and the rate of CO2 assimilation in leaves were higher. In plants with At4g20990 gene knockout, the same characteristics changed in opposite ways relative to wild type. Possible mechanisms of the participation of α-CA2 and α-CA4 in photosynthetic reactions are discussed, taking into account that protons can be either consumed or released in the reactions they catalyze.

Carbonic anhydrases in photosynthetic cells of higher plants.

This review presents information about carbonic anhydrases, enzymes catalyzing the reversible hydration of carbon dioxide in aqueous solutions. The families of carbonic anhydrases are described, and data concerning the presence of their representatives in organisms of different classes, and especially in the higher plants, are considered. Proven and hypothetical functions of carbonic anhydrases in living organisms are listed. Particular attention is given to those functions of the enzyme that are relevant to photosynthetic reactions. These functions in algae are briefly described. Data about probable functions of carbonic anhydrases in plasma membrane, mitochondria, and chloroplast stroma of higher plants are discussed. Update concerning carbonic anhydrases in chloroplast thylakoids of higher plants, i.e. their quantity and possible participation in photosynthetic reactions, is given in detail.

[Helicobacter pylori infection in patients with functional dyspepsia, eradication rates in the Donetsk region].

We have investigated 175 patients with Rome III diagnostic criteria for functional dyspepsia. 104 (59%) patients were infected with Helicobacter pylori. Three-component (PPI + clarithromycin + amoxicillin) 7-day therapy with the addition of the probiotic was effective in 92 patients (88.4%). Dyspeptic symptoms were resolved in 37 patients with successful eradication (40%). Persistent effect for 6 months was maintained in 24 patients (26%). Eradication efficacy in eliminating of the dyspepsia symptoms was higher in epigastric pain syndrome than postprandial distress syndrome.

[Results of the cardiosurgical care delivery to teenagers and adult patients with inborn heart failures].

The results of surgical aid delivery to adult patients, suffering inborn heart failures (IHF), were analyzed for period of 15 yrs. Surgical activity in teenagers and adult patients, suffering IHF, was analyzed with the objective to create a management strategy for such patients. More close dispensary control of teenagers in a pediatrician-cardiologist was noted, while their transfer to the adult clinic it is necessary to follow them thoroughly together with dispensary registration. Progressive enhancement of the patients quantity was registered, in whom more complex and combined inborn anomalies of the heart and the main vessels were diagnosed. Enhancement of the interventions quantity, which are performed on the exit tract of left ventricle and ascending aorta and what is caused by introduction of angiographic interventions, was noted together with total rising of the operations quantity.

The size of the light-harvesting antenna of higher plant photosystem II is regulated by illumination intensity through transcription of antenna protein genes.

In arabidopsis plants, with an increase in illumination intensity during growth the extent of reduction of the plastoquinone pool in the photosynthetic electron transport chain increased, whereas the effective quantum yield of photosynthesis decreased. After 5 days of growth under high illumination intensity, these parameters in high light returned to values observed in "shade-adapted" plants in low light. During the same period, the size of the antenna decreased, correlating with a decrease in the amounts of proteins of peripheral pigment-protein complexes. It was found that the decrease in the amounts of these proteins occurred due to suppression of transcription of their genes.

[Correction of secondary hyperlipidemia in children with diabetes].

At 25 children with insulin dependent diabetes mellitus (IDDM, Type I) in a decompensation stage was estimated efficiency of correction secondary hyperlipidemia. The basic group (n = 11) along with insulinotherapy and a lipotrophic preparation (methionine) accepted probiotic Bifiform Baby in a dose 0.5 g once a day within 10 days; the comparison group (n = 14) received insulin therapy and a lipotrophic preparation. Efficiency of treatment was estimated on dynamics of lipids in serum of blood: triglycerides (TG), the total cholesterol (TH), betalipoproteides (BLP). At patients of the basic group authentic decrease TG, TH, BLP was established; at patients of group of comparison--decrease TH and BLP. Advantage of therapy secondary hyperlipidemia at children with diabetic ketoacidosis with use of Bifiform Baby consists in the elimination of the main infringement of a metabolismus of lipids in the given disease--hypertriglyceridemia.

[Features of the physical development of children with pathology of the gastrointestinal disorders and trophological disorders].

This article presents the results of the physical development analysis of 15,230 students of St. Petersburg according to automated complex dispensary examination (ACDE). Was found a correlation between physical development and age dynamics of gastrointestinal diseases and trophological disorders.

Heterogeneous origin of carbonic anhydrase activity of thylakoid membranes.

Carbonic anhydrase activities of pea thylakoids as well as thylakoid fragments enriched either in Photosystem 1 (PS1-membranes) or Photosystem 2 (PS2-membranes) were studied. The activity of PS1-membranes if calculated on chlorophyll basis was much higher than the activity of PS2-membranes. Acetazolamide, a non-permeable inhibitor of carbonic anhydrases, increased carbonic anhydrase activity of PS2-membranes at concentrations lower than 10(-6) M and suppressed this activity only at higher concentrations. A lipophilic inhibitor of carbonic anhydrases, ethoxyzolamide, effectively suppressed the carbonic anhydrase activity of PS2-membranes (I50 = 10(-9) M). Carbonic anhydrase activity of PS1-membranes was suppressed alike by both inhibitors (I50 = 10(-6) M). In the course of the electrophoresis of PS2-membranes treated with n-dodecyl-beta-maltoside "high-molecular-mass" carbonic anhydrase activity was revealed in the region corresponding to core-complex of this photosystem. Besides, carbonic anhydrase activity in the region of low-molecular-mass proteins was discovered in the course of such an electrophoresis of both PS2- and PS1-membranes. These low-molecular-mass carbonic anhydrases eluted from corresponding gels differed in sensitivity to specific carbonic anhydrase inhibitors just the same as PS1-membranes versus PS2-membranes. The results are considered as evidence for the presence in the thylakoid membranes of three carriers of carbonic anhydrase activity.

Photosystem II associated carbonic anhydrase activity in higher plants is situated in core complex.

The thylakoid membrane containing photosystem II (PSII membranes) from pea and wheat leaves catalyzed the reaction of CO2 hydration with low rate, which increased after their incubation either with Triton X-100, up to Triton/chlorophyll ratio 1:1, or 1 M CaCl2. The presence of the inhibitor of CAs, p-aminomethylbenzensulfonamide (mafenide), at the start line in the course of electrophoresis of PSII membranes solubilized by n-dodecyl-beta-maltoside (DM) decreased the amount of PSII core complex in the gel. The elution of PSII core complex from the column with immobilized mafenide occurred only either by mafenide or another inhibitor of CAs, ethoxyzolamide. The above results led to a conclusion that membrane-bound CA activity associated with PSII is situated in the core complex.

[The creation of a "new" aorta and the characteristics of coronary artery transplantation in the anatomical correction of transposition of the great vessels of the heart]. / Sozdanie "novoi" aorty i osobennosti peresadki koronarnykh arterii pri anatomicheskoi korrektsii transpozitsii magistral'nykh sosudov serdtsa.

Opinion varies among surgeons regarding the preferable method of surgical correction in patients with transposition of the great vessels (TGV) presenting with abnormal origins of the coronary artery (CA) and inadequacies in the diameters of the great vessels. In the work presented, the experience is analyzed gained with the performing of TGV radical correction operations of the arterial switch type in patients presenting with anomalous CA origins and abnormalities in diameters of the great vessels (n = 5). The submitted option permits simplifying CA transfer avoiding the coronary blood flow-related fatal intraoperative complications. Our modification of creation of a "new" aorta combined with particular features of implantation of the coronary arteries enabled us to secure good results in the presence of malformed, TGV, anatomy, which fact allowed the arterial switch operations to be extended to a greater number of cases in disadvantageous anatomical variants of TGV.

[The experience of application of peritoneal dialysis in cardiosurgery of an early age children]. / Opyt ispol'zovaniia peritoneal'nogo dializa v kardiokhirurgii detei rannego vozrasta.

The experience of the peritoneal dialysis (PD) successful application in 16 children of an early age was summarized. In all the patients the terminal state with an acute cardiac and renal insufficiency was noted after cardiosurgical operation conduction using artificial blood circulation. The improvement of hemodynamical indices, renal and pulmonary function as well, were promoted by an early application of PD.

[Forlax in the treatment of chronic constipation of different origins]. / Forlaks v lechenii khronicheskogo zapora razlichnogo geneza.

The greatest benefit from forlax occurs in patients with constipation of various etiology. The highest effect was recordable in those patients with functional pathology of the intestinal tract who experienced increased frequency of their stools and alleviation of clinical manifestations of the illness to the greatest degree. In patients with decompensated cirrhosis normalization of stool was accompanied by a significant improvement in general condition and what is more, clinical manifestations of portal-systemic encephalopathy tended to be less pronounced. Relatively lower benefit from treatment with forlax occurred in those patients presenting with an appreciable decline in propulsive activity of the intestines against the background of severe diabetes mellitus. The therapy of these patients was supplemented with prokinetics in the belief that this measure will help in achieving stable normalization of stool.

[The anatomy of coronary arteries in the main vascular transposition]. / Anatomiia venechnykh arterii pri transpozitsii magistral'nykh sosudov.

The analysis of the coronary arteries (CA) morphology in the main vessels transposition (MVT) cases was presented, basing on the clinical and cadaveric investigations data. During performance of 88 operations there were revealed 8 different morphological variants of the CA branching. There were studied 8 heart macropreparations with MVT and there were disclosed 4 different types of the CA morphology.

[The surgical treatment of aortic coarctation in infants in the 1st year of life with significantly decreased contractile function of the left ventricle]. / Khirurgicheskoe lechenie koarktatsii aorty u detei pervogo goda zhizni so znachitel'no snizhennoi sokratitel'noi funktsiei levogo zheludochka.

During the 1991-1998 yrs period there were operated on 20 patients with coarctation of aorta (CoA), aged up to 1 year old, in whom the ejaculatory fraction (EF) of left ventriculus (LI) was not exceeding 40%. All the patients were admitted to the hospital in the severe state of health, the end-diastolic (EDI) and the end-systolic (ESI) index constituting accordingly (144.2 +/- 47.2) and (84.2 +/- 34.7) ml/m2, EF LV--(34.2 +/- 4.7)%. After the operation one patient have died, the mortality was 5%. In three to eight days after the operation EDV have lowered by 15% on average, EF LV--have enhanced by 20%. In the majority of children in the late follow-up period EDI and ESI have lowered significantly, approximating the normal level, EF LV have normalized. The significant lowering of the LV function and the extremely severe state of the patient with CoA are not contraindications to the surgical intervention performance, vice versa, to such the patients the intensive therapy and the urgent operation conduction are indicated. The CoA demolition not only saves the child's life but fully normalizes the LV function in majority of the patients.

[The myocardial defense in the arterial bypass surgery]. / Zashchita miokarda pri vypolnenii operatsii arterial'nogo perekliucheniia.

The experience of myocardium protection during performance of operation of arterial switching (AS) in 57 patients was analyzed. In 30 patients cardioplegic solution was infused at one moment, what had advantages in comparison with its multimoment infusion.