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1.
Klin Lab Diagn ; (4): 13-6, 2013 Apr.
Artículo en Ruso | MEDLINE | ID: mdl-23984547

RESUMEN

The need in study and interpretation of eosinophilograms in children with allergic diseases is conditioned by higher rate of eosinophilia, large specter of morphologic and functional signs of eosinophils, dependence of indicators from character of clinical course of disease, presence of complications and schemes of treatment. The level of laboratory techniques applied in routine clinical practice to evaluate morphological and functional characteristics of eosinophils is not developed enough. The implementation of such modern high-tech techniques as computer morphometry, highly sensitive and highly specific modifications ELISA, which are applied to detect associated with eosinophilia cytokines, chemokines and growth factors make it possible to approach to this issue at the new qualitative level.


Asunto(s)
Eosinofilia/sangre , Eosinófilos/patología , Hipersensibilidad/sangre , Adolescente , Niño , Citocinas/sangre , Eosinofilia/complicaciones , Eosinofilia/patología , Eosinófilos/metabolismo , Femenino , Humanos , Hipersensibilidad/complicaciones , Hipersensibilidad/diagnóstico , Hipersensibilidad/patología , Masculino
2.
Klin Lab Diagn ; (8): 45-7, 2010 Aug.
Artículo en Ruso | MEDLINE | ID: mdl-20886720

RESUMEN

The need to verify sensitization to mycotic allergens in children with allergic bronchopulmonary diseases is due to the severity of their clinical course, the high frequency of complications, and the inadequate efficiency of conventional treatment regimens. The sensitivity of most techniques used in clinical practice to estimate the level of specific artibodies to mycotic antigens is inadequately high. Recently clinically introduced high-technology diagnostic methods allow one to attack the problem at a qualitatively new level. The application of one of these methods, namely the highly sensitive semiautomatic diagnostic technology ImmunoCAP, permits the determination of the rates of IgE- and IgG-associated specific immune reactions in children with allergic respiratory diseases.


Asunto(s)
Alveolitis Alérgica Extrínseca/sangre , Antígenos Fúngicos/inmunología , Aspergilosis Broncopulmonar Alérgica/sangre , Asma/sangre , Pruebas Inmunológicas/métodos , Adolescente , Alveolitis Alérgica Extrínseca/inmunología , Aspergilosis Broncopulmonar Alérgica/inmunología , Asma/inmunología , Niño , Preescolar , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina G/sangre , Hongos Mitospóricos/inmunología
3.
Med Parazitol (Mosk) ; (3): 11-5, 2010.
Artículo en Ruso | MEDLINE | ID: mdl-20873373

RESUMEN

The paper describes a rare manifestation of Blastocystis hominis infection in a 3-month-old baby with food allergy that appeared as atopic dermatitis and coloproctitis induced by food proteins (cow's milk, hen's egg). The fact that food allergy may be one of the predisposing factors in the development of clinical manifestations of B. hominis infection is discussed. The assumption that the amoeboid form of blastocysts accompanies the clinical manifestation of the infection is confirmed.


Asunto(s)
Infecciones por Blastocystis/diagnóstico , Blastocystis hominis/aislamiento & purificación , Dermatitis Atópica/complicaciones , Hipersensibilidad al Huevo/complicaciones , Huevos/efectos adversos , Hipersensibilidad a la Leche/complicaciones , Leche/efectos adversos , Proctocolitis/complicaciones , Animales , Infecciones por Blastocystis/complicaciones , Pollos , Heces/parasitología , Humanos , Lactante , Masculino
4.
Vestn Otorinolaringol ; (5): 20-2, 2009.
Artículo en Ruso | MEDLINE | ID: mdl-20037550

RESUMEN

This study included a total of 90 patients of whom 35 ones suffered chronic hypertrophic (CHR) and chronic vasomotor (CVR) rhinitis. All the patients were examined in the saccharine test and underwent morphometry for the measurement of the ciliary beating rate (CBR) and the magnitude of extrusion of ciliary epithelium. Saccharine transit time in the group of CHR patients was found to vary from 20 to 43 minutes and CBR to equal 3.75 Hz. All these patients showed enhanced epithelial extrusion. Saccharine transit time in patients with CVR increased up to 17-29 min, with CBR being decreased to a mean of 4.56 Hz in 33 (60%) of them. In 22 (40%) cases of CVR, CBR remained normal. Interestingly, patients with CVR exhibited no signs of epithelial atrophy whereas most of them had reduced CBR.


Asunto(s)
Depuración Mucociliar/fisiología , Mucosa Nasal/patología , Rinitis Vasomotora/fisiopatología , Biopsia , Enfermedad Crónica , Endoscopía , Humanos , Hipertrofia , Mucosa Nasal/fisiopatología , Rinitis Vasomotora/patología
6.
Klin Lab Diagn ; (11): 21-3, 2000 Nov.
Artículo en Ruso | MEDLINE | ID: mdl-11486741

RESUMEN

The density and morphology of blood eosinophils from 60 children with atopic dermatitis were investigated. The proportion of hypodense eosinophils is higher during the acute stage of disease than during remission (59.78 +/- 0.20% vs. 21.40 +/- 0.04%).


Asunto(s)
Dermatitis Atópica/sangre , Eosinofilia/sangre , Eosinófilos/patología , Separación Celular , Centrifugación por Gradiente de Densidad , Niño , Preescolar , Dermatitis Atópica/complicaciones , Eosinofilia/etiología , Humanos
8.
Klin Lab Diagn ; (1): 9-10, 1994.
Artículo en Ruso | MEDLINE | ID: mdl-8032743

RESUMEN

Effects of various concentrations of dalargin on quantitative parameters of peripheral blood E-rosette-forming cells were studied in vitro. The drug had a stimulating effect in concentrations 10(-2)-10(-4) and 10(-12)-10(-14) micrograms/ml and an inhibiting effect in concentrations 10(-6)-10(-10) micrograms/ml. The drug had no effect in concentrations 10(-5) and 10(-11) micrograms/ml. Biphasic nature of dalargin effect permit the authors recommend it as an immunomodulating agent. The findings helped develop an individualized approach to prescription of dalargin therapy with due consideration of the initial counts of immunocompetent cells.


Asunto(s)
Adyuvantes Inmunológicos/farmacología , Leucina Encefalina-2-Alanina/análogos & derivados , Linfocitos/inmunología , Leucina Encefalina-2-Alanina/farmacología , Humanos , Técnicas In Vitro , Linfocitos/efectos de los fármacos , Formación de Roseta
9.
Klin Lab Diagn ; (3): 38-9, 1994.
Artículo en Ruso | MEDLINE | ID: mdl-7921819

RESUMEN

Lymphocyte reactivity was assessed with various surface antigens detectable by Russian monoclonal antisera by indirect immunofluorescence in peripheral blood samples from 40 healthy children in vitro in loading tests. Values of a coefficient reflecting individual reactivity of immunocompetent cells with surface antigens CD5, CD4, and CD8 corresponding to type II reaction were found characteristic of children whatever their age or sex. The results may be used as reference values in examinations of children with various diseases.


Asunto(s)
Inmunocompetencia , Niño , Femenino , Humanos , Técnicas In Vitro , Masculino , Valores de Referencia , Formación de Roseta
11.
Pediatriia ; (7): 18-21, 1991.
Artículo en Ruso | MEDLINE | ID: mdl-1721695

RESUMEN

The purpose of the study was to analyze the character of the expression of the blood lymphocyte epitopes SD4, SD8 (EBLE SD4, SD8) in a series of the loading in-vitro tests in children suffering from the nephrotic syndrome, with different HLA haplotypes. Nine children with the hormone-sensitive nephrotic syndrome (HSNS) and hormone-resistant nephrotic syndrome (HRNS) and 11 parents were examined. Before and after the in-vitro loading with medicamentous agents EBLE SD4, SD8 were determined by flow cytofluorometry, while HLA antigens were tested by the standard micro-lymphocytotoxic method. The studies allowed revealing differences in the responses of EBLE SD8 to the in-vitro loading in children with the HRNS and HSNS. The character of EBLE SD4, SD8 in a child with the NS and its parents may attest to the involvement of those antigens in the pathogenetic component of the given disease.


Asunto(s)
Antígenos CD4/inmunología , Antígenos CD8/inmunología , Antígenos HLA-A/genética , Antígenos HLA-B/genética , Antígenos HLA-DR/genética , Síndrome Nefrótico/inmunología , Linfocitos T/inmunología , Adolescente , Adulto , Antígenos CD4/genética , Antígenos CD8/genética , Niño , Preescolar , Epítopos/genética , Epítopos/inmunología , Femenino , Humanos , Hidrocortisona/farmacología , Técnicas In Vitro , Levamisol/farmacología , Masculino , Síndrome Nefrótico/genética , Padres , Fenotipo , Linfocitos T/efectos de los fármacos
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