RESUMEN
The phytochemical investigation of Trichodesma amplexicaule guided by bioassay, to isolation of triterpenoids and aliphatic phytoconstituents. To evaluate the anti-inflammatory activities of isolated phytoconstituents, models with carrageenan-induced acute arthritis and complete Freund's adjuvant (CFA)-induced chronic arthritis in rats were conducted. The investigated results showed that alkanoic acid significantly inhibited the carrageenan-induced acute arthritis. Moreover, alkane also supressed the development of chronic arthritis induced by CFA. It has been reported that alkane was the major phytoconstituent (1.03 +/- 0.00135%) in in vivo studies.
Asunto(s)
Alcanos/farmacología , Antiinflamatorios/farmacología , Artritis Experimental/tratamiento farmacológico , Boraginaceae/química , Triterpenos/farmacología , Alcanos/química , Alcanos/uso terapéutico , Animales , Antiinflamatorios/uso terapéutico , Bioensayo/métodos , Modelos Animales de Enfermedad , Edema/inducido químicamente , Edema/tratamiento farmacológico , Adyuvante de Freund/toxicidad , Indometacina/farmacología , Indometacina/uso terapéutico , Masculino , Ratas , Ratas Wistar , Triterpenos/química , Triterpenos/uso terapéuticoRESUMEN
The hexane extract of Arnebia hispidissima yielded a mixture of naphthaquinones: arnebin-1, arnebin-7, tiglic acid (ester of dihydroxy alkannin), alkannin, arnebinol and cycloarnebin-7. To evaluate the anti-inflammatory activities of hexane extract and isolated naphthaquinones, models with carrageenan-induced acute arthritis and complete Freund's adjuvant (CFA)-induced chronic arthritis in rats were conducted. The observed results indicated that pretreatment with cycloarnebin-7 significantly inhibited the carrageenan-induced acute arthritis. Moreover, arnebin-1, significantly suppressed the development of chronic arthritis induced by CFA. The present study deals with the quantification of naphthaquinones from in vivo and in vitro cell cultures of plant species and isolated compounds from intact plant tested for their swelling inhibitory potency. It has been reported that arnebin-1 was the major naphthaquinone both in vivo (0.62%) and in vitro (0.27%) cell cultures.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Artritis/prevención & control , Boraginaceae , Edema/prevención & control , Fitoterapia , Extractos Vegetales/farmacología , Animales , Antiinflamatorios no Esteroideos/administración & dosificación , Antiinflamatorios no Esteroideos/uso terapéutico , Artritis/inducido químicamente , Carragenina , Relación Dosis-Respuesta a Droga , Edema/inducido químicamente , Adyuvante de Freund , Masculino , Extractos Vegetales/administración & dosificación , Extractos Vegetales/uso terapéutico , Quinonas/administración & dosificación , Quinonas/farmacología , Quinonas/uso terapéutico , Ratas , Ratas WistarRESUMEN
Arnebia hispidissima ethanolic extract, after chromatography, yielded a number of shikonin derivatives, which were identified as arnebin-5, arnebin-6, teracryl shikonin, arnebinone and acetyl shikonin. All these compounds were firstly reported from this plant species and evaluated to the anti-inflammatory activity of ethanolic extract and isolated shikonin derivatives, models with carrageenan-induced paw edema and complete Freund's adjuvant (CFA)-induced chronic arthritis in rats were conducted. The observed results indicated that pre-treatment with arnebinone significantly inhibited the carrageenan-induced paw edema and also suppressed the development of chronic arthritis induced by CFA.
Asunto(s)
Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/farmacología , Boraginaceae/química , Extractos Vegetales/farmacología , Plantas Medicinales/química , Animales , Antiinflamatorios/química , Artritis/inducido químicamente , Artritis/tratamiento farmacológico , Edema/inducido químicamente , Edema/tratamiento farmacológico , Etanol , Masculino , Estructura Molecular , Naftoquinonas/química , Naftoquinonas/aislamiento & purificación , Naftoquinonas/farmacología , Extractos Vegetales/química , Raíces de Plantas/química , Ratas , Ratas WistarRESUMEN
The anti-inflammatory and antimicrobial activities of the 95% ethanol extract, benzene fraction and isolated triterpenoids of Strobilanthes callosus were investigated. In the carrageenan-induced paw edema inflammation model, the taraxerol showed a high reduction of edema, but the antimicrobial effect observed was lower at the two doses employed. These results confirm the use of this plant in folk medicine as an anti-inflammatory and antimicrobial herbal drug.
Asunto(s)
Acanthaceae , Antiinfecciosos/farmacología , Antiinflamatorios/uso terapéutico , Edema/prevención & control , Hongos/efectos de los fármacos , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Fitoterapia , Extractos Vegetales/farmacología , Triterpenos/farmacología , Animales , Antibacterianos , Antiinflamatorios/administración & dosificación , Carragenina , Relación Dosis-Respuesta a Droga , Edema/inducido químicamente , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/administración & dosificación , Extractos Vegetales/uso terapéutico , Ratas , Ratas Wistar , Triterpenos/administración & dosificación , Triterpenos/uso terapéuticoRESUMEN
Five pyrrolizidine alkaloids have been isolated from Heliotropium subulatum and tested for their antimicrobial activity.
