RESUMEN
The biological and molecular properties of a new formulation of Amphotericin B complexed with the surfactant palmitoyl mannose were studied in in vitro as well as in in vivo situations. The properties analyzed include toxicity towards two types of mammalian cells and four fungi strains, effect on macrophage activity, inflammatory properties, acute toxicity in mice and spectral behavior in presence of foetal calf serum or 6% propanol. The results demonstrate that, in presence of palmitoyl mannose, the cytotoxicity of AmB is decreased towards both, fungal and mammalian cells while its fungistatic potential is increased, its inflammatory properties are conserved and its acute toxicity is significantly diminished. These effects can be potentially explained by the formation of a complex between AmB and the sugar ester that impedes the interaction of the drug with either serum components or cell membrane constituents. The overall properties of AmB in the complex would be expected to favor an increase in the immunoadjuvant properties of the drug, a more localized inflammation during fungal infection and consequently a better therapeutic efficiency.
Asunto(s)
Anfotericina B/farmacología , Detergentes/farmacología , Macrófagos Peritoneales/efectos de los fármacos , Manosa/análogos & derivados , Anfotericina B/administración & dosificación , Anfotericina B/química , Animales , División Celular/efectos de los fármacos , Línea Celular , Detergentes/química , Peróxido de Hidrógeno/metabolismo , Manosa/síntesis química , Manosa/farmacología , Ratones , Ratones Endogámicos BALB C , EspectrofotometríaRESUMEN
Ten medically or environmentally significant strains of bacteria were assessed as to their metallic content (Be, Mg, Cr, Mn, Fe, Co, Cu, Zn, Cd and Pb). Metallic detection was performed using a carbon rod-equipped atomic absorption spectrophotometer. Experimental parameters such as heavy metal content of initial culture medium, incubation, "red fuming" nitric acid digestion times, evaporative losses (and their individual correction) plus primary sample dilutions, were standardized. For the ten strains studied, characteristic metallic profiles were observed. More work is needed to assess the taxonomic and epidemiologic significance of metallic profiles of microorganisms.