[Isolation of the lipid-transporting protein Ns-LTP1 from seeds of the garden fennel flower (Nigella sativa)].

A novel lipid-transporting protein (Ns-LTP1) has been isolated from seeds of the garden fennel flower Nigella sativa. The molecular mass, N-terminal amino acid sequence, and amino acid composition of the protein have been determined. Ns-LTP1 has a molecular mass of 9602 Da and contains eight cysteine residues which form four disulfide bridges. The protein is capable of suppressing the development of some phytopathogenic fungi and oomycetes.

[Cardiotropic activity of total bufadienolides from the poison of Central Asian toad Bufo viridis]. / Kardiotropnaia aktivnost' summy bufadienolidov iz iada sredneaziatskoi zhaby Bufo viridis.

The sum of bufadienolides (bacagin) isolated from the poison of Bufo viridis toad occurring in Central Asia produces a selective strophanthin-K-like action upon the heart function, increasing myocardial contractility, retarding cardiac rhythm, and positively influencing the parameters of myocardial metabolism. The mechanism of realization of the positive ionotropic effect of bacagin is probably related to the ability of increasing the basal level of cytosol calcium at the expense of inhibiting the activity of Na+/K(+)-ATPase and, to some extent, Ca(2+)-ATPase in endoplasmic reticulum.

[Peptide endotoxins and disruption of the hemostasis system during a complicated pregnancy]. / Peptidnye éndotoksiny i narushenie sistemy gemostaza pri oslozhnennom techenii beremennosti.

Peptide fractions, affecting the state of the hemostasis system, were isolated from blood plasma of patients with abnormal development of pregnancy. The functional activity of homogeneous peptide preparation was expressed as induction of ADP-dependent thrombocytes aggregation.

[Methods of diagnosis and correction of endogenous intoxication in obstetrical suppurative-inflammatory diseases]. / Metody diagnostiki i korrektsii éndogennoi toksikatsii pri gnoino-vospalitel'nykh zabolevaniiakh v akusherskoi praktike.

Content of middle molecular mass peptides was studied in blood of patients with pyo-inflammatory diseases. The peptide fraction was detected exhibiting a membrano-tropic effect, which correlated with the rate of endogenous intoxication. A procedure of autotransfusion of UV-irradiated blood was used in the course of detoxication.

[Amino acid sequence of orientotoxins I and II from the venom of the hornet Vespa orientalis]. / Aminokislotnaia posledovatel'nost' orientotoksinov I i II iz iada shershnia Vespa orientalis.

Orientotoxin I, a neurotoxin of presynaptic effect having a lysophospholipase activity, and orientotoxin II, a highly toxic phospholipase A2, were isolated from the hornet Vespa orientalis venom, and their primary structures were determined. Despite their different functional activity, orientotoxin I and II proved to be structural homologues, differing significantly in the amino acid sequence from well-known toxic phospholipase from other sources.

[Toxic components of the venom of the cellar spider Segestria florentina]. / Izuchenie toksicheskikh komponentov iada pogrebnogo pauka Segestria florentina.

Two neurotoxins and one insectotoxin have been isolated from venom of the cellar spider Segestria florentina, their homogeneity being proved by disk electrophoresis, isoelectric focusing, and analysis of N-terminal amino acid residues. The neurotoxins are polypeptides with molecular mass about 5000 D. For the insectotoxin, containing 35 amino acid residues with molecular mass 3988 D, the total primary structure is established.

[Approach to elaborating immunochemical methods for removing peptides from the blood of kidney failure patients]. / Podkhod k razrabotke immunokhimicheskikh metodov dlia udaleniia peptidov iz krovi bol'nykh pochechnoi nedostatochnost'iu.

A homogenic peptide (m. w. 2100 dalton) was isolated from blood serum of patients suffering from renal failure by gel chromatography on a Sephadex G-25 column and by partition chromatography on a column with cellulose MN-300. The peptide was not detectable in the blood of healthy donors. The amino acid composition of the peptide was studied. To obtain an immunosorbent, the peptide was conjugated by the bifunctional reagent, glutaric aldehyde. An optimal conjugation variant promoting the formation of conjugates with a molecular weight of 60000 dalton was developed. The molecular weight of the conjugates was measured by gel chromatography on a Sephadex column G-50 and with the aid of disc electrophoresis in polyacrylamide gel.