rClca2 is associated with epidermal differentiation and is strongly downregulated by ultraviolet radiation.

BACKGROUND: Excessive skin exposure to solar radiation damages proteins and DNA, ultimately leading to skin ageing and cancers. OBJECTIVES: To identify new ultraviolet B (UVB) target genes to understand the mechanisms behind the detrimental effects of UVB. METHODS: Organotypic, stratified cultures of rat keratinocytes were exposed to UVB and analysed using a genome-wide expression array, quantitative real-time polymerase chain reaction and histology. The most downregulated gene, rClca2, was further characterized in rat keratinocytes and mouse skin models. RESULTS: A single, 30 mJ cm(-2) dose of broadband UVB proved effective in the organotypic epidermal culture. The expression of 627 genes was changed 24 h postirradiation. In silico analysis of the data indicated activation of DNA repair, metabolism, cell cycle control and amino acid metabolism, but only limited inflammation under these conditions. We selected for further investigation the most downregulated gene, rClca2, previously suggested to regulate keratinocyte differentiation and adhesion, and found that UVB caused a long-lasting downregulation in its expression. Both the rClca2 full-length isoform (expressed in the differentiating cells) and the truncated isoform (expressed in the basal layers) were reduced by UVB. Immunohistochemistry of mouse skin samples with isoform-specific antibodies showed a similar, epidermal differentiation-related pattern. In mouse specimens exposed to chronic ultraviolet radiation (UVR) the staining intensities were reduced and the differentiation-related isoform was disturbed in the hyperplastic and carcinomatous areas induced by UVR. CONCLUSIONS: The data show that rClca2 is a novel UVB target gene and suggest that it might play a role in epidermal differentiation and UV-dependent skin malignancies.

The quality of life and social support in significant others of patients with breast cancer--a longitudinal study.

The purpose of this study was to evaluate prospectively the quality of life (QOL) and received social support from the network and nurses in significant others of breast cancer patients and identify factors predicting negative changes in their QOL within 6 months. The quasi-random longitudinal study conducted for the breast cancer patients and their significant others. Patients were quasi-randomised to supportive intervention group (via telephone at baseline and face-to-face at follow-up) and control group. This paper reports results of significant others (N = 165). The QOL data were collected using the Quality of Life Index - Cancer Version (QLI-CV). Support from network in aid increased the risk of negative changes in health and functioning. Retired significant others had a greater risk of more negative changes in their global and in socio-economic QOL than other. Relatives had a smaller risk to negative changes both in their global and in their family QOL than spouses/partners/boyfriends of patients with breast cancer. QOL of the significant others should be supported more intensively and enhanced by the use of individually tailored methods on the basis of significant others and their family needs.

Associations of health, dietary and job-related factors, and mental problems with cardiovascular diseases in aging.

BACKGROUND: While cardiovascular diseases are prevalent in aging population, associations between different factors and cardiovascular diseases are less studied or inconsistency still exists. AIM: To determine which factors measured at baseline predicted overall cardiovascular diseases and hypertension, and which factors were currently associated with these conditions. METHODS: Based on a sample of aging and retired food industry employees (N = 100, mean age 62 years) associations of health, dietary and job-related factors and mental problems with overall cardiovascular morbidity and hypertension were assessed with independent samples t-test, chi2 test and binary logistic regression analyses. Data were derived from health examinations and self-completed questionnaires in 1989 and 2000. RESULTS: Added medical findings, related to cardiovascular diseases, appeared as predictor of overall cardiovascular morbidity, while elevated blood pressure together with high systolic and diastolic pressure, dyslipidemia and elevated blood pressure together, added medical findings, and inability to enjoy daily activities predicted hypertension. According to our analyses most significant current associations with overall cardiovascular morbidity were feeling of weakness, dyspnoea, hopelessness about the future, cardiac arrhythmia, chronic mental stress and lower HDL cholesterol, and with hypertension metabolic syndrome, lower HDL cholesterol, hopelessness about the future and muscular fatigue on exertion. CONCLUSIONS: Health factors were most important predictors of hypertension during a long-term period, while health factors together with mental problems had significant current associations with overall cardiovascular morbidity including hypertension.

Factors associated with premature departure from working life among ageing food industry employees.

AIM: To find out how health status, work ability and job-related factors were associated with premature departure from working life in an 11 year follow-up period. METHODS: The study population was composed of 126 ageing food industry employees. Baseline data were derived from medical examinations and self-completed questionnaires in 1989. The follow-up data were collected in 2000. Data analyses were performed by independent samples t-tests and Mann-Whitney and chi(2) tests. RESULTS: Several chronic diseases, higher degree of work impairment due to diseases, long-term sickness absence, stress symptoms, low scores in work ability index, poor subjective work ability and physical work ability, and heavy physical workload seemed to be factors associated with early departure from working life among ageing food industry employees. CONCLUSION: Identification of these factors, prevention of sickness and promotion of work ability seem to be the most important tools to reduce premature exit from working life.