RESUMEN
This study investigated the actual status of constipation. In total, 978 female students in Korea participated. We identified the relationship among constipation and life style, clothing patterns, housing patterns, dietary habits, and dietary intake in a constipation symptom group and a normal group. The actual constipation rate based on the Rome II criteria was 27.0% (n = 264). Body weight (p < 0.05) and body mass index (p < 0.05) in the constipation group were significantly higher than those in the normal group. The incidence of functional bowel disease and irritable bowel syndrome in the constipation group were significantly higher than those in the normal group. The discomfort of wearing underwear was significantly higher in the constipation group than that in the normal group. The constipation group revealed a significantly higher rate of irregular dietary habits than those in the normal group. The dietary diversity score of the normal group was 4.22 (p < 0.05), which was significantly higher than that of constipation group (4.12). No significant difference in life style factors was observed. It is necessary for university female students to correct their dietary habits, maintain food intake of three times per day, and select diverse foods. Furthermore, it is necessary for university female students to wear comfortable clothing to lessen symptoms and improve constipation rates.
Asunto(s)
Femenino , Humanos , Índice de Masa Corporal , Peso Corporal , Vestuario , Estreñimiento , Ingestión de Alimentos , Conducta Alimentaria , Vivienda , Incidencia , Síndrome del Colon Irritable , Corea (Geográfico) , Estilo de Vida , Ciudad de RomaRESUMEN
No abstract available.
RESUMEN
A harmonious relationship between doctors and their patients establishes a firm foundation for good practice. Factors that affect such a relationship include communication skills and the language used. Essentially, the language should be clear and comprehensible for both parties. Doctors usually tend to use technical jargons. Since Korean doctors were educated with English textbooks, they are inclined to teach their students in English terms. Their use of old medical terms makes communication with their patients very difficult. Such language impediment complicates matters when doctors have to answer the patients' questions and ask detailed questions about their condition. And it is not only in the use of English where communication problems arise between doctors and patients. Most of these problems can also be traced to the use of old medical terminologies originating from the difficult Chinese characters. The Korean medical community has been conducting a campaign to replace existing difficult medical terms with plain Korean, Hangul terms. Particularly, members of the board of medical education are expected to make active use of the new terms in their teaching fields, in writing research papers, and in their clinical practice.
Asunto(s)
Humanos , Pueblo Asiatico , Educación Médica , EscrituraRESUMEN
BACKGROUND: Evaluation of clinical performance is an essential area of Medical Licensure Examination. Yet, clinical skills has been neither uniformly well taught nor adequately assessed. This study was done as a part of preliminary study to introduce clinical performance assessment to Korean Medical Licensure Examination. The goals of this study were to identify the potential logistic problems and to examine the feasibility of OSCE to Medical Licensure Examination. METHODS: 48 minutes long OSCE, which consist of eight 5-minute stations, was developed. The same examination was administered to students over a four-week period. RESULTS: Total 26 volunteer fourth-year medical students were tested. 11 students were examined at the first administration and the rest of them were evaluated four-week later. Cronbach alpha of the total stations was 0.52. Any big administration problems were not revealed. There was no significant total score difference between early examinee and later ones. Students evaluated the OSCE contents and process positively. CONCLUSION: To acquire more reliable and valid examination, we need to develop more objective checklist and evaluator and SP training.
Asunto(s)
Humanos , Lista de Verificación , Competencia Clínica , Licencia Médica , Estudiantes de Medicina , VoluntariosRESUMEN
BACKGROUND: Evaluation of clinical performance is an essential area for the students who are preparing their career as a medical doctor. However the clinical skills has neither been taught with balanced proportion nor adequately being assessed their abilities. Yet no significant studies on this issue has been reported in Korea so far. The present study was done as a part of preliminary study to seek a model that would be introduced clinical performance assessment in Korean Medical Licensure Examination system. Purpose: The objectives of this study is to develop items of clinical performance and the effective methods to carry out the clinical performance assessment in Korean Medical Licensure Examination system. METHODS: Primary list of clinical performances which was generated through discussion between researchers and medical experts has been distributed to the physicians of various levels. Six hundred ninety seven medical doctors including professors, family physicians, and public health doctors were asked to express their opinion by mail questionnaire. Final items were selected after the reaction of questionnaires and each item has been carefully studied its validity, achievability, and practicality. RESULTS: Thirty five items were selected among fifty one items presented according to the checked number of "essential" and "frequently useful". Seven items were added according to the higher rate of recommendation by open questionnaire. The forty two items were confirmed as a final list which the students should be expected to demonstrate prior to graduation from undergraduate medical school("exit objective"). The OSCE was performed without any serious logistic problems and both examinees and evaluators thought the experience positively. CONCLUSION: To look at the present environment and the readiness it is recommendable to apply the items of psychomotor skills at the beginning for Medical Licensure Examination. For that purpose various medical dolls, kits and subjects can be used.
Asunto(s)
Humanos , Competencia Clínica , Corea (Geográfico) , Licencia Médica , Médicos de Familia , Servicios Postales , Salud Pública , Encuestas y CuestionariosRESUMEN
Using in situ hybridization technique with digoxigenin-labelled riboprobe, study on the expression of hsp 70 mRNA in the developing mouse brain was performed. The results obtained are as follows; 1. In embryonic day 16 group, cells with strong reactivity to hsp70 mRNA were found in spinal cord. In neuroepithelial layer lining fourth ventricle and external granular layer of cerebellum, moderate reactivity was observed. But the reactivity was weak in the forebrain including cerebral cortex, diencephalon and olfactory bulb. 2. In embryonic day 18 group, the regional pattern of hsp70 mRNA expression was similar to that of embryonic day 16 group. In medulla oblongata, however, stronger reactivity was found in the embryonic day 18 group. 3. In postnatal day 0 mice group, cells with moderate or strong reactivity to hsp70 mRNA were found in the overall area of central nervous system, Especially, cells with moderate reactivity were found in the dentate gyrus of hippocampus, and the supragranular cortical plate and subplate neocortex. 4. In postnatal day 2 mice group, cells with moderate or strong reactivity to hsp70 mRNA were found in the same pattern as in postnatal day 0 group. Further differentiation of cerebral cortex and cerebellum was found. 5. Strong expression of hsp70 mRNA was found in the areas with high rate of cell division. In general, the area of expression moved to more rostral area in central nervous system as development proceeds. Above results suggest that hsp70 play an important role in the development and differentiation of central nervous system.
Asunto(s)
Animales , Ratones , Encéfalo , División Celular , Sistema Nervioso Central , Cerebelo , Corteza Cerebral , Giro Dentado , Diencéfalo , Cuarto Ventrículo , Proteínas de Choque Térmico , Hipocampo , Calor , Proteínas HSP70 de Choque Térmico , Hibridación in Situ , Bulbo Raquídeo , Neocórtex , Bulbo Olfatorio , Prosencéfalo , ARN Mensajero , Médula EspinalRESUMEN
Cell proliferation index in the thyroid is regarded as an important marker for predicting the prognosis and for differential diagnosis of thyroid tumors. Among the methods for the detection of cell proliferation in histological sections, immunohistochemistry for PCNA and BrdU are the most preferable ones. In this study, we evaluated the regeneration process of the thyroid in the rat after surgical resection with special respect to the cell proliferation revealed by both PCNA and BrdU immunohistochemistry, and compared these two results. Rat thyroid was resected bilaterally, about 50~60% of the total lobe in volume, and the tissues were obtained 1, 2, 3, 4, 5, and 7 days after operation, immunohistochemistry was done, and positive and negative cells were counted. The regeneration pattern reflected by cell proliferation was basically same in both PCNA and BrdU immunohistochemistry, except that the positive ratio for PCNA was 4~6 fold higher than that for BrdU in general. In the control thyroid, positive cells were rare. However, the ratio of positive cells increased immediately after the operation, on day 1, and reached at its peak on day 2. The high ratio sustained until day 4, after which the ratio declined abruptly. Through the experimental days, positive cells were more crowded in the area adjacent to the resection plane than elsewhere, suggesting that the areas are more active regenerating focus. Although the pattern of proliferation was same both in PCNA and BrdU immunostaining in terms of time and localization, BrdU staining was easier to read and seeded to reflect cell prolifer-ations more specifically than PCNA staining.
Asunto(s)
Animales , Ratas , Bromodesoxiuridina , Proliferación Celular , Diagnóstico Diferencial , Inmunohistoquímica , Pronóstico , Antígeno Nuclear de Célula en Proliferación , Regeneración , Glándula TiroidesRESUMEN
Angiogenesis plays a fundamental role in development of circulation system, reorganization of reproductive system, wound healing. Pathological angiogenesis is deeply involved in a variety of diseases, particularly solid tumor growth and metastasis. However, it is not easy to study the mechanism of angiogenesis because endothelial cells proceed complex differentiation by interaction with extracellular matrix proteins and growth factors. However, human umbilical vein endothelial cells (HUVEC) form polygonal networks of capillary-like tubes in 3D Matrigel cultures. Differentiation of endothelial cells will be observed accurately by application of videomicroscopy. Thrombospondin-1 is secreted by a wide variety of cells including endothelial cells and is incorporated into their matrix. Thrombospondin-1 can modulate differentiation of endothelial cells by increasing cell-cell interactions as well as cell-substrate interactions. The current study was undertaken to determine which mechanism is involved in inhibition of angiogenesis by Thrombospondin-1. They was secreted from HUVEC during the process of angiogenesis in 3D Matrigel culture. When applied to endothelial cells attachment to the surface of Matrigel was not decreased, but spreading was decreased. In addition, bigger clusters was formed by enhancement of cell to cell binding by Thrombospondin-1. They inhibit cord and tube formation of HUVEC by inhibition of migration. These results suggest that Thrombospondin-1 inhibits angiogenesis by blocking differentiation of endothelial cells to motile phenotype in 3D Matrigel culture.
Asunto(s)
Técnicas de Cultivo de Célula , Células Endoteliales , Proteínas de la Matriz Extracelular , Células Endoteliales de la Vena Umbilical Humana , Péptidos y Proteínas de Señalización Intercelular , Microscopía por Video , Metástasis de la Neoplasia , Neovascularización Patológica , Fenotipo , Cicatrización de HeridasRESUMEN
The carbonic anhydrase II (CA-II) is specifically expressed in oligodendrocytes, the cells responsible for myelination in the central nervous system. However no direct evidence on relationship between myelin formation and CA-II immunoreactivity has been described. The aims of these studies are to investigate the relationship between CA-II and myelination during cerebellar development of mouse. Myelin staining was found on postnatal (P) 14, and its intensity increased in proportion to developmental age. CA-II positive oligodendrocytes were observed in the white matter of cerebellum on P 14 day. CA-II positive oligoden-drocytes also occured in the granular layer and Purkinje cell layers in the later stage of dvelopment. The parallel development in the CA-II expression and myelination during development suggests that CA-II in oligoendrocyte play a role to myelination.
Asunto(s)
Animales , Ratones , Carbono , Anhidrasa Carbónica II , Anhidrasas Carbónicas , Sistema Nervioso Central , Cerebelo , Vaina de Mielina , OligodendroglíaRESUMEN
The pattern of distribution in rat spinal cord and changing pattern during normal aging of c-Fos, Bcl-2, Bax, and p53 expression were investigated by immunohistochemical staining. Male Sprague-Dawley rats at the age of one week, five months, and two years were studied. C-Fos immunoreactivity was observed diffusely in gray matters in neonatal rats, preferentially located in deep dorsal horn and around central canal. Compared with those of neonatal rats, immunoreactive cells decreased prominently in adult rats. In aged rats, these cells were not seen in any segments. In a transverse section, spatial expression of Bcl-2 and Bax proteins showed a diffuse distribution pattern with immunore-activity more prominent in the anterior horn. Continuing expression of these proteins was shown in each age group. In adult rats, Bcl-2 immunoreactivity was decreased drastically compared to that of neonatal rats. The immunoreactivity was higher in aged than in adult rats, but the number of immunoreactive cells was not different between aged and neonatal rats. The number of Bax-immunoreactive cells was greater in adult than in neonatal rats; in aged rats, it was similar with that of adult rats. The spinal cords of neonatal rats were not p53-immunoreactive, though p53-positive cells were detected in all segments of adult spinal cord. P53-positive cells were stained along the cellular margin, with a pale central portion. The pattern of p53 immunoreactivity in adult and aged rats was similar; the number of p53-positive cells, however, was higher in aged rats than in adult. In conclusion, we demonstrated that the expression patterns of c-Fos, Bcl-2, Bax, and p53 proteins in rat spinal cord change during normal aging for the first time.
Asunto(s)
Adulto , Animales , Humanos , Masculino , Ratas , Envejecimiento , Proteína X Asociada a bcl-2 , Cuernos , Ratas Sprague-Dawley , Médula EspinalRESUMEN
Nitric oxide (NO) involvement has been demonstrated in mechanisms of synaptic plasticity, particularly in hippocampal long-term potentiation, a mechanism that underlies certain forms of learning and memory. Further, NO has been shown to regulate various neurotransmitters which play an important role in learning and memory. Several findings suggest that NO production may be decreased in the aged rat. Changes in the nNOS-containing neurons with aging were demonstrated by immunocytochemistry and in situ hybridization. NOS-immunoreactive cells in aged rats were present in all cortical areas and the hippocampus, and the pattern of distribution was similar to that of the control group. The number of NOS-immunoreactive cells in the cerebral cortex was significantly decreased in the aged rats, but the extent of changes was variable in each area, and ranged from mild decrease (50%). Severely decreased areas were the cingulate cortex, parietal cortex area 1, temporal cortex area 1, 2, 3, medial part of occipital cortex area 2, monocular and binocular part of occipital cortex area 1, entorhinal cortex, hippocampus proper, dentate gyrus and subiculum. Moderately decreased areas (30~50%) were frontal cortex area 1, 2, 3, parietal cortex area 2, forelimb, hindlimb, lateral part of occipital cortex area 2. Slightly decreased area was insular cortex. Morphologically, the number of dendritic branches seemed to be decreased in aged group and the length of dendrites of NOS-IR neurons showed a tendency to shorten. These results indicate the involvement of neuronal system containing NOS in the aging brain, and provide the first morphological evidence for the loss of NOS neurons in the cerebral cortex of the aged rats by immunocytochemistry. Further multidisciplinary investigations involving normal aging and neurodegenerative disease such as Alzheimer's disease are needed to clarify the importance of nitric oxide changes in the cerebral cortex with aging.
Asunto(s)
Animales , Ratas , Envejecimiento , Enfermedad de Alzheimer , Encéfalo , Corteza Cerebral , Dendritas , Giro Dentado , Corteza Entorrinal , Miembro Anterior , Giro del Cíngulo , Miembro Posterior , Hipocampo , Inmunohistoquímica , Hibridación in Situ , Aprendizaje , Potenciación a Largo Plazo , Memoria , Enfermedades Neurodegenerativas , Neuronas , Neurotransmisores , Óxido Nítrico , Plásticos , Rabeprazol , TelescopiosRESUMEN
No abstract available.
RESUMEN
To determine whether computer aided sessions can effectively replace some of the labor intensive laboratories in human gross anatomy, second-year medical students at the College of Medicine, Chung-Ang University were randomly assigned to either a traditional cadaver-dissection or self-learning using intensive computer programs of gross anatomy covering the same materials. After finishing the six-week anatomy course covering the upper and lower extremities, two groups of students were tested in written and practical examinations. Even though they were tested on an actual cadaver, in a statistical analysis, the performance of thirty students in the computer-lab were not significantly different from sixty-five students group in the dissecting lab. It strongly suggestes that part of traditional gross anatomy laboratory can be replaced to a digitalized laboratory.
Asunto(s)
Humanos , Cadáver , Aprendizaje , Extremidad Inferior , Estudiantes de MedicinaRESUMEN
Guanine aminohydrolase (GAH; Guanine deaminase, EC 3.5.4.3) is an enzyme that has a role in purine catabolism. This enzyme produces xanthine and ammonia by hydrolysis of guanine, and xanthine is further degraded to uric acid and hydrogen peroxide by another enzyme, xanthine oxidase. Most of the enzymes involved in purine catabolism have been studied for their biological functions, physiological roles and amino acid sequences, and biochemical activity of GAH is known to be detected in various organs such as liver, kidney, small intestine and brain. Its activity is also known to be changed during brain development. In this study, we hoped to reveal expression pattern of GAH in developing rat brain by western blotting and immunohistochemistry. In western blotting, GAH immunoreactivity was not detected on 14-, 16- and 18-days-old fetal rat brains. Its reactivity was first detected from 20-days-old fetal rat brain and highly increased after birth. And it was maintained at steady level from 2 weeks after birth. In immunohistochemistry, no positive cells were found on 14- and 16-days-old fetal rat brain sections. A few GAH-immunoreactive cells appeared from 18-days-old fetal rat brain and they were localized at olfactory bulb, cerebral cortex, midbrain, pons and medulla. The 20-days-old fetal rat brain also showed immunoreactive cells at hippocampus and the staining intensity was still weak. Postnatal 2-days-old rat brain also showed immunoreactive cells at basal ganglia and the number of positive cells and staining intensity were increased. Thereafter, immunoreactivity appeared on many neuronal cells around various areas in the brain and nerve fibers also showed reactivity on postnatal brains. The number of positive cells decreased from 1 week after birth and a few positive cells were observed on olfactory bulb and cerebellum from 2 weeks after birth. In mature brain most of GAH were localized on nerve fibers and few positive cells could be found on olfatory bulb only. From these, we can suspect that GAH may have some functional relationship with nerve fibers.
Asunto(s)
Animales , Ratas , Secuencia de Aminoácidos , Amoníaco , Ganglios Basales , Western Blotting , Encéfalo , Cerebelo , Corteza Cerebral , Guanina Desaminasa , Guanina , Hipocampo , Esperanza , Peróxido de Hidrógeno , Hidrólisis , Inmunohistoquímica , Intestino Delgado , Riñón , Hígado , Mesencéfalo , Metabolismo , Fibras Nerviosas , Neuronas , Bulbo Olfatorio , Parto , Puente , Ácido Úrico , Xantina , Xantina OxidasaRESUMEN
PURPOSE: We compared pathogen recovery rates by obtaining two blood cultures instead of one blood culture containing 1ml and collecting a larger volume, 1 to 3ml. METHODS: Total of 750 blood specimens from 250 patients with fever, a temperature higher than 39degrees C and suspected bacteremia were obtained. Each patient had two samples of blood, A (1ml) and B (4ml), obtained at 30-minute interval from separate sites of extremities and B was divided into B1 (1ml) and B2 (3ml). Each sample was inoculated into aerobic culture media. Patients were excluded if two samples of blood were not obtained or if the isolate represented a contaminant. RESULTS: A pathogen was isolated in 19 (7.6%) of 250 patients and 37 (4.9%) of 750 specimens. In 7 patients, the pathogen was isolated with all the culture methods and in 12 patients, one or more of the cultures yielded no growth. The pathogen recovery rates were 53% (10/19) in A and B1, 89% (17/19) in B2 and 68% (13/19) in A+B1. No difference was detected between A or B1 and A+B1 (P>0.05) and the pathogen recovery rate for B2 was significantly greater than that for A or B1 (P<0.05), but no significant differences were found in pathogen recovery when B2 was compared with A+B1. CONCLUSION: Increasing volume of blood from 1 to 3ml inoculated into blood culture bottles improves detection of bacteremia in pediatric patients and spares patients the cost and pain of an additional venipuncture.
Asunto(s)
Niño , Humanos , Bacteriemia , Medios de Cultivo , Extremidades , Fiebre , FlebotomíaRESUMEN
PURPOSE: We compared pathogen recovery rates by obtaining two blood cultures instead of one blood culture containing 1ml and collecting a larger volume, 1 to 3ml. METHODS: Total of 750 blood specimens from 250 patients with fever, a temperature higher than 39degrees C and suspected bacteremia were obtained. Each patient had two samples of blood, A (1ml) and B (4ml), obtained at 30-minute interval from separate sites of extremities and B was divided into B1 (1ml) and B2 (3ml). Each sample was inoculated into aerobic culture media. Patients were excluded if two samples of blood were not obtained or if the isolate represented a contaminant. RESULTS: A pathogen was isolated in 19 (7.6%) of 250 patients and 37 (4.9%) of 750 specimens. In 7 patients, the pathogen was isolated with all the culture methods and in 12 patients, one or more of the cultures yielded no growth. The pathogen recovery rates were 53% (10/19) in A and B1, 89% (17/19) in B2 and 68% (13/19) in A+B1. No difference was detected between A or B1 and A+B1 (P>0.05) and the pathogen recovery rate for B2 was significantly greater than that for A or B1 (P<0.05), but no significant differences were found in pathogen recovery when B2 was compared with A+B1. CONCLUSION: Increasing volume of blood from 1 to 3ml inoculated into blood culture bottles improves detection of bacteremia in pediatric patients and spares patients the cost and pain of an additional venipuncture.
Asunto(s)
Niño , Humanos , Bacteriemia , Medios de Cultivo , Extremidades , Fiebre , FlebotomíaRESUMEN
GH3 cells are derived from rat pituitary tumor cells that secrete prolactin and growth hormone, and important in studying prolactin-secreting pitutary tumors. This study was performed to examine the effects of polylysine on growth and differentiation of GH3 cells by means of (a) cell attachment assay (b) cell count and bromodeoxyuridine labeling and (c) immunohistochemistry for prolactin in the absence or presence of epidermal growth factor (EGF). Cell shape, attachment to the culture surface and growth of GH3 cells were not affected by polylysine coating. The percentages of prolactin-immunoreactive cells were higher in the cells cultured on the polylysine-coated surface compared to those on the plastic surface. Cell number and BrdU incorporation were lower in the EGF-treated cells on both culture surfaces. The results provided basic information on the effects of polylysine coating on GH3 cells in culture and suggested that polylysine coating was useful for the study on GH3 cells because it enhanced cell differentiation as well as it provided stronger attachment than plastic surfaces.
Asunto(s)
Animales , Ratas , Bromodesoxiuridina , Recuento de Células , Diferenciación Celular , Forma de la Célula , Factor de Crecimiento Epidérmico , Hormona del Crecimiento , Inmunohistoquímica , Neoplasias Hipofisarias , Plásticos , Polilisina , ProlactinaRESUMEN
In mammals, the ovary proceeds follicular development and atresia during the reproductive cycle. Follicular atresia occurs through apoptosis, programmed cell death (PCD) which is mediated by gene controlling apoptosis and cell cycle. Regulatory genes of apoptosis and cell cycle have previously reported. Recently, the products of bcl-2, bax, Fas, and TNF-a genes were reported to play critical roles in apoptosis, and the products of p21, p27, p57, and p53 genes were known to play many roles in cell cycle. In these genes, bcl-2 is known as a apoptosis inhibitor in the ovary, while p57 which was recently reported to express in the testis and other several organs, is a cyclin dependent kinase inhibitor (CDI). We thought that these genes are associated with follicular development and atresia. Therefore, the present study used a in situ hybridization to examine the expression of these two genes, followed by synthesis of a radioisotope labelled cRNA probes during PMSG-induced follicular development and atresia in the immature rat ovary. Morphological changes were occurred with the manner of 5 days periodicity after PMSG treatment. 1 or 2 days after PMSG treatment, the ovaries have showed growing follicles with antrum and healthy granulosa cells. The ovaries of 3 days after treatment were appeared continuous growing follicles and some degenerating follicles which had thinner granulosa cell layers than growing follicles. But the ovaries of 4 or 5 days have showed degenerating follicles which have pyknotic nuclei and thin distorted granulosa cell layers. These atretic follicles showed positive reaction with DNA end labelling method indicating apoptotic processes. In situ hybridization signals to p57 cRNA probe were expressed in the theca externa cells of the control ovary. In PMSG treated 4 or 5 days ovaries, the atretic follicles have showed stronger signals than 1 or 2 days growing ovarian follicles. But the signals to bcl-2 cRNA probe were expressed in the ovarian theca and interstitial cells including theca basement membrane. And this signals were decreased in the 4 or 5 days atretic follicles and interstitium. According to these data, we suggest that these two genes are associated to follicular development and atresia via individual up- and down regulatory mechanism.
Asunto(s)
Animales , Femenino , Ratas , Apoptosis , Membrana Basal , Ciclo Celular , Muerte Celular , Ciclinas , ADN , Atresia Folicular , Genes bcl-2 , Genes p53 , Genes Reguladores , Células de la Granulosa , Hibridación in Situ , Mamíferos , Folículo Ovárico , Ovario , Periodicidad , Fosfotransferasas , ARN Complementario , ARN Mensajero , Testículo , Células TecalesRESUMEN
The technique of in situ hybridization using synthetic oligonucleotides labelled by non-radioactive method was developed to localize vasoactive intestinal polypeptide, arginine-vasopressin and oxytocin mRNAs in the rat brain. Also double in situ hybridization technique where combination of non-radioactive and radioactive probes were applied was developed to localize 2 neuropeptide mRNAs in single tissue section. The results were as follows; In non-radioactive in situ hybridization methods using digoxigenin-labelled oligonucleotide probe, alkaline-phosphates method using NBT and BCIP as substrates gave the best result that specific hybridization signals were observed. In radioactive in situ hybridization methods using 35S-labelled oligonucleotide probe, specific hybridization signals were observed in both nuclear track emulsion and X-ray film autoradiography. In double in situ hybridization methods using combination of 35S-labelled and digoxigenin-labelled oligonucleotide probes, specific hybridization signals were observed in the group where K5 emulsion was applied as nuclear track emulsion. The technique of in situ hybridization using digoxigenin-labelled oligonucleotide applied in this study will be useful as alternative for radioactive in situ hybridization technique. Moreover, combination of non-radioactive and radioactive labelled probes in double in situ hybridization technique will be a useful tool for the simultaneous localization of various mRNAs in single section for the study of various neurotransmitters, neuropeptides, receptors and signal transduction molecules.