The genetically encoded calcium indicator GCaMP3 reveals spontaneous calcium oscillations at asexual stages of the human malaria parasite Plasmodium falciparum.

Most protocols used to study the dynamics of calcium (Ca2+) in the malaria parasite are based on dyes, which are invasive and do not allow discrimination between the signal from the host cell and the parasite. To avoid this pitfall, we have generated a parasite line expressing the genetically encoded calcium sensor GCaMP3. The PfGCaMP3 parasite line is an innovative tool for studying spontaneous intracellular Ca2+ oscillations without external markers. Using this parasite line, we demonstrate the occurrence of spontaneous Ca2+ oscillations in the ring, trophozoite, and schizont stages in Plasmodium falciparum. Using the Fourier transform to fluorescence intensity data extracted from different experiments, we observe cytosolic Ca2+ fluctuations. These spontaneous cytosolic Ca2+ oscillations occur in the three intraerythrocytic stages of the parasite, with most oscillations occurring in the ring and trophozoite stages. A control parasite line expressing only a GFP control did not reveal such fluctuations, demonstrating the specificity of the observations. Our results clearly show dynamic, spontaneous Ca2+ oscillations during the asexual stage in P. falciparum, independent from external stimuli.

Comparing the long-term persistence of different Wolbachia strains after the release of bacteria-carrying mosquitoes.

This paper proposes a bidimensional modeling framework for Wolbachia invasion, assuming imperfect maternal transmission, incomplete cytoplasmic incompatibility, and direct infection loss due to thermal stress. Our model adapts to various Wolbachia strains and retains all properties of higher-dimensional models. The conditions for the durable coexistence of Wolbachia-carrying and wild mosquitoes are expressed using the model's parameters in a compact closed form. When the Wolbachia bacterium is locally established, the size of the remanent wild population can be assessed by a direct formula derived from the model. The model was tested for four Wolbachia strains undergoing laboratory and field trials to control mosquito-borne diseases: wMel, wMelPop, wAlbB, and wAu. As all these bacterial strains affect the individual fitness of mosquito hosts differently and exhibit different levels of resistance to temperature variations, the model helped to conclude that: (1) the wMel strain spreads faster in wild mosquito populations; (2) the wMelPop exhibits lower resilience but also guarantees the smallest size of the remanent wild population; (3) the wAlbB strain performs better at higher ambient temperatures than others; (4) the wAu strain is not sustainable and cannot persist in the wild mosquito population despite its resistance to high temperatures.

Changes in K+ Concentration as a Signaling Mechanism in the Apicomplexa Parasites Plasmodium and Toxoplasma.

During their life cycle, apicomplexan parasites pass through different microenvironments and encounter a range of ion concentrations. The discovery that the GPCR-like SR25 in Plasmodium falciparum is activated by a shift in potassium concentration indicates that the parasite can take advantage of its development by sensing different ionic concentrations in the external milieu. This pathway involves the activation of phospholipase C and an increase in cytosolic calcium. In the present report, we summarize the information available in the literature regarding the role of potassium ions during parasite development. A deeper understanding of the mechanisms that allow the parasite to cope with ionic potassium changes contributes to our knowledge about the cell cycle of Plasmodium spp.

Bem-estar na escola e percepção da discriminação de gênero por meninas / School well-being and perception of gender discrimination by girls / Bienestar en la escuela y percepción de la discriminación de género por las niñas

Este artigo analisa, a partir da pesquisa "Ser Menina no Brasil", o bem-estar na escola e a (pouca) percepção da discriminação de gênero por meninas de 6 a 14 anos. Foram utilizados os dados quantitativos da Amostra-Escola, que envolve 1.609 meninas, de 21 municípios brasileiros, referente à dimensão "Escola e Escolarização" e que aponta a percepção das meninas sobre a frequência às aulas, os motivos de faltar à escola, os níveis de aprovação e reprovação, a interrupção dos estudos, os motivos de expulsão, as tarefas escolares, as relações de gênero no contexto escolar e a satisfação e bemestar na escola. A análise indicou uma percepção favorável ao bem-estar na escola e relações de gênero benéficas para as meninas. Estudos assim devem focar o contexto educativo, o qual possibilita a inserção e a construção de um lugar de pertencimento e a ruptura com uma cultura de desigualdade social que ainda mascara a exclusão. (AU)

This article analyzes, from the research "Being Girl in Brazil", the welfare in the school and the (low) perception of the gender discrimination by girls of 6 to 14 years. We used the quantitative data of the School-Sample, involving 1,609 girls from 21 Brazilian municipalities, referring to the "School and Schooling" dimension and which points out the girls perception about the attendance at school, reasons for missing school, levels of approval and disapproval, interruption of studies, reasons for expulsion, school tasks, gender relations in the school context, and school satisfaction and well-being. The analysis indicated a favorable perception of well-being in school and gender relations beneficial to girls. Such studies should focus on the educational context, which enables the insertion and construction of a place of belonging and the rupture with a culture of social inequality that still masks exclusion. (AU)

Este artículo analiza, a partir de la investigación "Ser niña en Brasil", el bienestar en la escuela y la (poca) percepción de la discriminación de género por niñas de 6 a 14 años. Hemos utilizado los datos cuantitativos de la Muestra-Escuela, que involucra a 1.609 niñas, de 21 municipios brasileños, referente a la dimensión "Escuela y Escolarización" y que apunta la percepción de las niñas sobre la frecuencia a las clases, los motivos de faltar a la escuela, los niveles de aprobación y reprobación, la interrupción de los estudios, los motivos de expulsión, las tareas escolares, las relaciones de género en el contexto escolar y la satisfacción y bienestar en la escuela. El análisis indicó una percepción favorable al bienestar en la escuela y relaciones de géneros beneficiosos para las niñas. Los estudios así deben enfocar el contexto educativo, lo cual posibilita la inserción y la construcción de un lugar de pertenencia y la ruptura con una cultura de desigualdad social que aún enmascara la exclusión. (AU)

Molecular basis of synchronous replication of malaria parasites in the blood stage.

The search for host factors that leads to malaria parasite synchronization has been the focus of several laboratories. The host hormone melatonin synchronizes Plasmodium falciparum in culture by increasing the number of mature parasite stages through a PLC-IP3 activation. Melatonin signaling is linked to crosstalk between Ca2+-cAMP that results in PKA activation. Two other kinases, PfPK7 and PfeIK1, and the nuclear protein PfMORC that lacks melatonin sensitivity in the inducible knock-down parasites are also identified as part of the hormone-signal transduction pathways. Melatonin also modulates P. falciparum mitochondrial fission genes FIS1, DYN1, and DYN2 in a stage-specific manner. How these multiple molecular mechanisms are orchestrated to lead to parasite synchronization is a fascinating and opened biological question.

The Knockout for G Protein-Coupled Receptor-Like PfSR25 Increases the Susceptibility of Malaria Parasites to the Antimalarials Lumefantrine and Piperaquine but Not to Medicine for Malaria Venture Compounds.

Previously we have reported that the G protein-coupled receptor (GPCR)-like PfSR25 in Plasmodium falciparum is a potassium (K+) sensor linked to intracellular calcium signaling and that knockout parasites (PfSR25-) are more susceptible to oxidative stress and antimalarial compounds. Here, we explore the potential role of PfSR25 in susceptibility to the antimalarial compounds atovaquone, chloroquine, dihydroartemisinin, lumefantrine, mefloquine, piperaquine, primaquine, and pyrimethamine and the Medicine for Malaria Venture (MMV) compounds previously described to act on egress/invasion (MMV006429, MMV396715, MMV019127, MMV665874, MMV665878, MMV665785, and MMV66583) through comparative assays with PfSR25- and 3D7 parasite strains, using flow cytometry assays. The IC50 and IC90 results show that lumefantrine and piperaquine have greater activity on the PfSR25- parasite strain when compared to 3D7. For MMV compounds, we found no differences between the strains except for the compound MMV665831, which we used to investigate the store-operated calcium entry (SOCE) mechanism. The results suggest that PfSR25 may be involved in the mechanism of action of the antimalarials lumefantrine and piperaquine. Our data clearly show that MMV665831 does not affect calcium entry in parasites after we depleted their internal calcium pools with thapsigargin. The results demonstrated here shed light on new possibilities on the antimalarial mechanism, bringing evidence of the involvement of the GPCR-like PfSR25.

Isohemigossypolone: Antiophidic properties of a naphthoquinone isolated from Pachira aquatica Aubl.

We investigated the antiophidic properties of isohemigossypolone (ISO), a naphthoquinone isolated from the outer bark of the Pachira aquatic Aubl. The inhibition of phospholipase A2, coagulant, fibrinogenolytic, hemorrhagic and myotoxic activities induced by Bothrops pauloensis venom (Pb) was investigated. For this, we use samples resulting from the incubation of Pb with ISO in different concentrations (1:1, 1:5 and 1:10 w/w), we also evaluated the condition of treatment using ISO after 15 min of venom inoculation. The activities of phospholipase A2, coagulant, fibrinogenolytic, hemorrhagic and myotoxic induced by the B. pauloensis venom were significantly inhibited when the ISO was pre-incubated with the crude venom. For in vivo neutralization tests, the results were observed even when the ISO was applied after 15 min of inoculation of the venom or metalloprotease (BthMP). Also, to identify the inhibition mechanism, we performed in silico assays, across simulations of molecular coupling and molecular dynamics, it was possible to identify the modes of interaction between ISO and bothropic toxins BmooMPα-I, Jararacussin-I and BNSP-7. The present study shows that naphthoquinone isohemigossypolone isolated from the P. aquatica plant inhibited part of the local and systemic damage caused by venom proteins, demonstrating the pharmacological potential of this compound in neutralizing the harmful effects caused by snakebites.

Antiophidic activity of the secondary metabolite lupeol isolated from Zanthoxylum monogynum.

Previous studies have demonstrated the potential antiophidic activity of Zanthoxylum monogynum A.St.-Hil. a tree from the Rutaceae family native to South America. In this present contribution, we demonstrate the activity of the metabolite lupeol, a triterpenoid isolated from the stem bark of Z. monogynum against the harmful effects of the Bothrops alternatus venom. We investigated the antiophidic properties of lupeol, for this purpose, and use crude venom (Pb) incubated with lupeol in different concentrations, testing in vitro experiments and inoculated in mice for inhibitory evaluations in vivo. Besides, we tried to elucidate through the molecular dynamics the mechanism of action of lupeol with the bothropic thrombin-like toxin Jararacussin-I; the acidic phospholipase A2 toxin BthA-I from Bothrops jararacussu and the metalloproteinase toxin BmooMP-I from Bothrops moojeni. In our results, we demonstrated the potential inhibitory effect upon coagulant, phospholipasic and myotoxic activities of the bothropic venom, previously incubated with lupeol. We found that lupeol triterpenoid was able to partially inhibit local and systemic damage caused by snake venom toxins. Our in silico results demonstrate that lupeol is capable of interacting and altering the activity of the thrombin-like toxin Jararacussin-I, and capable of interacting with the BthA-I acidic PLA2, both toxins present in Bothrops snakes venom, thus demonstrating the pharmacological potential of this compound for the treatment of bothropic accidents.

Quando ser menina é ruim: percepções de gênero em crianças e adolescentes / Cuando ser niña es malo: percepciones de género en niños y adolescentes / When being a girl is bad: gender perceptions in children and adolescents

Resumo Este artigo é um recorte da pesquisa que analisou as percepções de meninas de 6 a 14 anos sobre os aspectos que facilitam e/ou impedem o desenvolvimento de suas habilidades e a garantia de seus direitos a partir do ambiente familiar, escolar, comunitário e social. O estudo teve abordagem plurimetodológica e abrangeu as cinco regiões do Brasil. Em particular, destaca-se a percepção de meninas a partir do exame das respostas a duas questões. Observou-se que as meninas argumentam os aspectos negativos de gênero a partir de quatro grandes fatores: (a) violência sexual, (b) comportamentos e estética, (c) relação com o corpo e sexualidade e (d) responsabilidades e restrição da liberdade. Em todos eles, os discursos das meninas confirmam o quanto as relações de poder são determinantes nas concepções, representações e práticas de gênero ainda hegemônicas na sociedade.

Resumen Este artículo es un recorte de la investigación que analizó las percepciones de niñas de 6 a 14 años sobre los aspectos que facilitan y/o impiden el desarrollo de sus habilidades y la garantía de sus derechos, desde el ambiente familiar, escolar, comunitario y social. El estudio tuvo un enfoque plurimetodológico y abarcó las cinco regiones de Brasil. En particular, se destaca la percepción de las niñas a partir del examen de las respuestas a dos cuestiones. Se observó que las niñas argumentan los aspectos negativos de género a partir de cuatro grandes factores: (i) violencia sexual, (ii) comportamientos y estética, (iii) relación con el cuerpo y sexualidad y (iv) responsabilidades y restricción de la libertad. En todos ellos, los discursos de las niñas confirman cuánto las relaciones de poder son determinantes en las concepciones, representaciones y prácticas de género aún hegemónicas en la sociedad.

Abstract This article is a clipping of a research that analyzed the perceptions of girls aged 6-14 on the aspects that facilitate and/or prevent the development of their abilities, and the guarantee of their rights, from their family, school, community and social environment. The study had a multi-methodological approach and covered Brazil's five regions. In particular, the girls' perception stands out based on the examination of their answers to two questions. It was observed that the girls argue negative aspects of gender from four big factors: (a) sexual violence, (b) behavior and aesthetics, (c) relations with the body and sexuality, and (d) responsibilities and freedom restriction. In all of them, the girls' discourses confirm how power relations are determinant in the conceptions, representations and practices of gender that are still hegemonic in society.

Plasmodium falciparum Knockout for the GPCR-Like PfSR25 Receptor Displays Greater Susceptibility to 1,2,3-Triazole Compounds That Block Malaria Parasite Development.

The search for new compounds with antimalarial activity is urgent, as resistance to ones in the classical drug, has already been described in more than one continent. Compounds derived from 1,2,3-triazoles are effective against parasites and bacteria. Here, we evaluated the potential antimalarial activity against the human malaria parasite Plasmodium falciparum in a culture of fifty-four triazole compounds derived from 1H-and 2H-1,2,3-triazole. We identified thirty-one compounds with potential antimalarial activity at concentrations in the micromolar order (µM) and IC50 values ranging from 2.80 µM (9) to 29.27 µM (21). Then, we selected some of these compounds to perform the same tests on the PfSR25- strain (knockout for P. falciparum G-protein coupled receptor-like, SR25). Our experiences with the PfSR25- strain showed that both compounds with higher antimalarial activity for the 3D7 strain and those with less activity resulted in lower IC50 values for the knockout strain. The cytotoxicity of the compounds was evaluated in human renal embryonic cells (HEK 293), using MTT assays. This demonstrated that the compounds with the highest activity (9, 13, 19, 22, 24, 29), showed no toxicity at the tested concentrations.

The Plasmodium falciparum eIK1 kinase (PfeIK1) is central for melatonin synchronization in the human malaria parasite. Melatotosil blocks melatonin action on parasite cell cycle.

Melatonin and its indoles derivatives are central in the synchronization of malaria parasites. In this research, we discovered that melatonin is unable to increase the parasitemia in the human malaria Plasmodium falciparum that lacks the kinase PfeIK1. The PfeIK1 knockout strain is a valuable tool in the screening of indol-related compound that blocks the melatonin effect in wild-type (WT) parasite development. The assays were performed by using flow cytometry with simultaneous labeling for mitochondria viability with MitoTracker Deep Red and nucleus staining with SYBR Green. We found that Melatotosil leads to an increase in parasitemia in P. falciparum and blocks melatonin effect in the WT parasite. Using microscopy imaging system, we found that Melatotosil at 500 nM is able to induce cytosolic calcium rise in transgenic PfGCaMP3 parasites. On the contrary, the compound Triptiofen blocks P. falciparum cell cycle with IC50 9.76 µM ± 0.6, inhibits melatonin action, and does not lead to a cytosolic calcium rise in PfGCaMP3 parasites. We also found that the synthetic indol-related compounds arrested parasite cycle for PfeIK1 knockout and (WT) P. falciparum (3D7) in 72 hours culture assays with the IC50 values slighting lower for the WT strain. We concluded that the kinase PfeIK1 is central for melatonin downstream signaling pathways involved in parasite cell cycle progression. More importantly, the indol-related compounds block its cycle as an upstream essential mechanism for parasite survival. Our data clearly show that this class of compounds emerge as an alternative for the problem of resistance with the classical antimalarials.

Estudo retrospectivo de ultrassonografias em felinos realizadas no Hospital Veterinário da Universidade Federal de Goiás - Regional Jataí no ano 2017 / Retrospective study of ultrasonography in felines performed at the Veterinary Hospital of The Federal University of Goiás - Jataí Regional in 2017 / Estudio retrospectivo de ultrasonografías en felinos realizados en el Hospital Veterinario de La Universidad Federal de Goiás - Regional Jataí en el año 2017

O uso do diagnóstico ultrassonográfico especialmente em pequenos animais vem se tornando uma prática cada vez mais comum na rotina clinica médico veterinária. O presente estudo teve como objetivo quantificar e analisar os exames ultrassonográficos realizados em pacientes felinos no Hospital Veterinário da UFG/REJ durante o ano de 2017. Por meio do levantamento dos dados constatou-se que foram realizados 208 diagnósticos por imagem por meio da ultrassonografia, onde 29,33%, 29%, 8% e 3% dos casos correspondem a patologias dos sistemas digestório, urinário, reprodutivo e respiratório respectivamente. Dessa forma este estudo retrospectivo permitiu identificar e quantificar, quais são os achados e as patologias mais frequente em felinos em nossa rotina clínica, demonstrando também a importância da aplicabilidade da ultrassonografia para o diagnóstico das enfermidades.(AU)

The use of ultrasonographic diagnosis, especially in small animals, has become an increasingly common practice in routine veterinary medical practice. The present study aimed to quantify and analyze the ultrasound examinations performed in feline patients at the UFG / REJ Veterinary Hospital during the year 2017. Through the data collection, it was verified that 208 diagnoses were performed per image through ultrasonography, where 29,33%, 29%, 8% and 3% of the cases correspond to diseases of the digestive, urinary, reproductive and respiratory systems, respectively. In this way, this retrospective study allowed to identify and quantify the most frequent findings and pathologies in cats in our clinical routine, also demonstrating the importance of the applicability of ultrasonography for the diagnosis of diseases.(AU)

El uso del diagnóstico ultrasonográfico especialmente en pequeños animales, se está convirtiendo en una práctica cada vez más común en la rutina clínica veterinaria. El presente estudio tuvo como objetivo cuantificar y analizar los exámenes ultrasonográficos realizados en pacientes felinos en el Hospital Veterinario de la UFG / REJ durante el año 2017. A través del levantamiento de los datos se constató que se realizaron 208 diagnósticos por imagen a través de la ultrasonografía, donde 29,33%, 29%, 8% y 3% de los casos corresponden a patologías de los sistemas digestivo, urinario, reproductivo y respiratorio respectivamente. De esta forma este estudio retrospectivo permitió identificar y cuantificar, cuáles son los hallazgos y las patologías más frecuentes en felinos en nuestra rutina clínica, demostrando también la importancia de la aplicabilidad de la ultrasonografía para el diagnóstico de las enfermedades.(AU)

Estudo retrospectivo de ultrassonografias em felinos realizadas no Hospital Veterinário da Universidade Federal de Goiás - Regional Jataí no ano 2017 / Retrospective study of ultrasonography in felines performed at the Veterinary Hospital of The Federal University of Goiás - Jataí Regional in 2017 / Estudio retrospectivo de ultrasonografías en felinos realizados en el Hospital Veterinario de La Universidad Federal de Goiás - Regional Jataí en el año 2017

O uso do diagnóstico ultrassonográfico especialmente em pequenos animais vem se tornando uma prática cada vez mais comum na rotina clinica médico veterinária. O presente estudo teve como objetivo quantificar e analisar os exames ultrassonográficos realizados em pacientes felinos no Hospital Veterinário da UFG/REJ durante o ano de 2017. Por meio do levantamento dos dados constatou-se que foram realizados 208 diagnósticos por imagem por meio da ultrassonografia, onde 29,33%, 29%, 8% e 3% dos casos correspondem a patologias dos sistemas digestório, urinário, reprodutivo e respiratório respectivamente. Dessa forma este estudo retrospectivo permitiu identificar e quantificar, quais são os achados e as patologias mais frequente em felinos em nossa rotina clínica, demonstrando também a importância da aplicabilidade da ultrassonografia para o diagnóstico das enfermidades.

The use of ultrasonographic diagnosis, especially in small animals, has become an increasingly common practice in routine veterinary medical practice. The present study aimed to quantify and analyze the ultrasound examinations performed in feline patients at the UFG / REJ Veterinary Hospital during the year 2017. Through the data collection, it was verified that 208 diagnoses were performed per image through ultrasonography, where 29,33%, 29%, 8% and 3% of the cases correspond to diseases of the digestive, urinary, reproductive and respiratory systems, respectively. In this way, this retrospective study allowed to identify and quantify the most frequent findings and pathologies in cats in our clinical routine, also demonstrating the importance of the applicability of ultrasonography for the diagnosis of diseases.

El uso del diagnóstico ultrasonográfico especialmente en pequeños animales, se está convirtiendo en una práctica cada vez más común en la rutina clínica veterinaria. El presente estudio tuvo como objetivo cuantificar y analizar los exámenes ultrasonográficos realizados en pacientes felinos en el Hospital Veterinario de la UFG / REJ durante el año 2017. A través del levantamiento de los datos se constató que se realizaron 208 diagnósticos por imagen a través de la ultrasonografía, donde 29,33%, 29%, 8% y 3% de los casos corresponden a patologías de los sistemas digestivo, urinario, reproductivo y respiratorio respectivamente. De esta forma este estudio retrospectivo permitió identificar y cuantificar, cuáles son los hallazgos y las patologías más frecuentes en felinos en nuestra rutina clínica, demostrando también la importancia de la aplicabilidad de la ultrasonografía para el diagnóstico de las enfermedades.

Estudio retrospectivo de ultrasonografías en felinos realizados en el hospital veterinario de la Universidad Federal de Goiás - Regional Jataí en el año 2017. / Retrospective study of ultrasonography in felines performed at the veterinary hospital of the Federal University of Goiás - Jataí Regional in 2017. / Estudo retrospectivo de ultrassonografias em felinos realizadas no hospital veterinário da Universidade Federal de Goiás Regional Jataí no ano 2017.

El uso del diagnóstico ultrasonográfico especialmente en pequeños animales, se está convirtiendo en una práctica cada vez más común en la rutina clínica veterinaria. El presente estudio tuvo como objetivo cuantificar y analizar los exámenes ultrasonográficos realizados en pacientes felinos en el Hospital Veterinario de la UFG / REJ durante el año 2017. A través del levantamiento de los datos se constató que se realizaron 208 diagnósticos por imagen a través de la ultrasonografía, donde 29,33%, 29%, 8% y 3% de los casos corresponden a patologías de los sistemas digestivo, urinario, reproductivo y respiratorio respectivamente. De esta forma este estudio retrospectivo permitió identificar y cuantificar, cuáles son los hallazgos y las patologías más frecuentes en felinos en nuestra rutina clínica, demostrando también la importancia de la aplicabilidad de la ultrasonografía para el diagnóstico de las enfermedades.

The use of ultrasonographic diagnosis, especially in small animals, has become an increasingly common practice in routine veterinary medical practice. The present study aimed to quantify and analyze the ultrasound examinations performed in feline patients at the UFG / REJ Veterinary Hospital during the year 2017. Through the data collection, it was verified that 208 diagnoses were performed per image through ultrasonography, where 29,33%, 29%, 8% and 3% of the cases correspond to diseases of the digestive, urinary, reproductive and respiratory systems, respectively. In this way, this retrospective study allowed to identify and quantify the most frequent findings and pathologies in cats in our clinical routine, also demonstrating the importance of the applicability of ultrasonography for the diagnosis of diseases.

O uso do diagnostico ultrassonográfico especialmente em pequenos animais, vem se tornando uma prática cada vez mais comum na rotina clinica médico veterinária. O presente estudo teve como objetivo quantificar e analisar os exames ultrassonográficos realizados em pacientes felinos no Hospital Veterinário da UFG/REJ durante o ano de 2017. Através do levantamento dos dados constatou-se que foram realizados 208 diagnósticos por imagem através da ultrassonografia, onde 29,33%, 29%, 8% e 3% dos casos correspondem a patologias dos sistemas digestório, urinário, reprodutivo e respiratório respectivamente. Dessa forma este estudo retrospectivo permitiu identificar e quantificar, quais são os achados e as patologias mais frequente em felinos em nossa rotina clínica, demonstrando também a importância da aplicabilidade da ultrassonografia para o diagnóstico das enfermidades.  

Subjetividades em trânsito: identidade, diáspora africana e cultura imaterial / Subjetividades en tránsito: la identidad, la diáspora y cultura inmaterial / Subjectivities in transit: identity, african diaspora and intangible culture

RESUMO A proposta do artigo pretende trazer contribuições para a valorização da memória oral da diáspora africana no contexto latino-brasileiro contemporâneo, com ênfase na relação entre identidade e diáspora africana do início do século XXI. Para o desenvolvimento do artigo foram utilizados os dados etnográficos coletados, entre os anos de 2013 e 2015, no Estado do Rio de Janeiro, com angolanos que vivem na capital carioca e têm interesse pela manutenção de uma memória oral da diáspora africana contemporânea no Brasil. Assim sendo, a potência das ideias e crenças foram analisadas para que se identifiquem as redes de movimento e reprodução social e subjetiva, que mobilizam os sujeitos envolvidos na pesquisa, enfatizando a importância da salvaguarda da memória atlântica contemporânea, para que não seja esquecida e nem silenciada.

RESUMEN El artículo propuesto tiene como objetivo reunir las contribuciones para la valorización de la memoria oral de la diáspora africana en el contexto contemporáneo latinobrasileño, con énfasis en la relación entre la identidad y la diáspora africana de principios del siglo XXI. Para el desarrollo del artículo se utilizaron datos etnográficos recogidos entre 2013 y 2015 en el Estado de Río de Janeiro con los angoleños que viven en la capital carioca y tienen un interés en mantener una memoria oral de la diáspora africana contemporánea en Brasil. Por lo tanto, se analizó el poder de las ideas y creencias con el fin de identificar las redes de circulación y reproducción social y subjetiva que movilizan los sujetos involucrados en la investigación, haciendo hincapié en la importancia de salvaguardar la memoria contemporánea del Atlántico, para que no se la olvide ni silencie.

ABSTRACT The article proposes to contribute to the valorization of the oral memory of the African diaspora in the contemporary Latin-Brazilian context, with emphasis on the relationship between identity and African diaspora of the beginning of the 21st century. Ethnographic data collected between the years of 2013 and 2015 in the State of Rio de Janeiro were used for the development of the article. Interviews with Angolans living in the capital of Rio de Janeiro and interested in maintaining an oral memory of the contemporary African diaspora in Brazil were conducted. Therefore, the power of ideas and beliefs were analyzed to identify the networks of social and subjective movement and reproduction that mobilize the subjects involved in the research, emphasizing the importance of safeguarding the contemporary Atlantic memory, so that it is neither forgotten nor silenced.

Insect gall occurrence in savanna and forest remnant sites of Hidrolândia, GO, Brazil Central

In this study we perform an inventory of the insect galls in savanna and forest sites of Hidrolândia, Goiás, Brazil. We found 150 insect gall morphotypes, distributed on 39 botanical families and 104 plant species. Among the insect galls, 81 gall morphotypes were recorded in the savanna site and 73 in the forest site. The plant taxa richest in insect galls were the family Fabaceae with 22 gall morphotypes, the genus Bauhinia (Fabaceae) with 15, and the species Siparuna guianensis (Siparunaceae) with seven gall morphotypes. We found gall-inducing insects belonging to orders Diptera, Coleoptera, Lepidoptera and Thysanoptera. The galling insects of family Cecidomyiidae (Diptera) were the most common inducing 48.1% of the gall morphotypes. This is the first systematic survey of insect galls realized in the city of Hidrolândia, being this the site with the higher insect gall diversity already cataloged to the Central region of Brazil.(AU)

Insect gall occurrence in savanna and forest remnant sites of Hidrolândia, GO, Brazil Central

In this study we perform an inventory of the insect galls in savanna and forest sites of Hidrolândia, Goiás, Brazil. We found 150 insect gall morphotypes, distributed on 39 botanical families and 104 plant species. Among the insect galls, 81 gall morphotypes were recorded in the savanna site and 73 in the forest site. The plant taxa richest in insect galls were the family Fabaceae with 22 gall morphotypes, the genus Bauhinia (Fabaceae) with 15, and the species Siparuna guianensis (Siparunaceae) with seven gall morphotypes. We found gall-inducing insects belonging to orders Diptera, Coleoptera, Lepidoptera and Thysanoptera. The galling insects of family Cecidomyiidae (Diptera) were the most common inducing 48.1% of the gall morphotypes. This is the first systematic survey of insect galls realized in the city of Hidrolândia, being this the site with the higher insect gall diversity already cataloged to the Central region of Brazil.

Impacto das ações formativas no uso de tecnologias nas práticas docentes / Training actions impact on the use of technologies in teaching practices / Impacto de las acciones formativas en el uso de tecnologías en las prácticas docentes

O estudo consiste em uma revisão sistemática sobre o impacto das ações de formação no uso de tecnologias nas práticas docentes, os principais efeitos e os desafios. A amostra é resultante do levantamento de artigos científicos completos indexados na base Scientific Electronic Library Online (SciELO), publicados entre 2003 e 2016. Com o objetivo de compreender a crítica das comunicações e de suas significações, foi realizada uma análise de conteúdo temática pautada nos estudos de Bardin (2002). A revisão apontou carência de efetivos resultados quanto aos impactos desejados em relação ao uso de tecnologias nas práticas docentes com foco pedagógico e com autoria por parte dos professores e estudantes. Assim, são necessários demais estudos que possam suprir as necessidades ainda vigentes, focando em práticas de utilização dos recursos tecnológicos em rede que potencializam a aprendizagem e o desenvolvimento dos estudantes.

The study is a systematic review on the impact of training actions on the use of technologies in teaching practices, its main effects, and challenges. The sample is the result of the survey of complete scientific articles indexed in the Scientific Electronic Library Online - (SciELO), published between 2003 and 2016. Seeking to understand communication and its meanings critically, we held a thematic content analysis, according to Bardin (2002). The review pointed to the lack of sufficient results regarding the desired impacts about the use of technologies in teaching practices with pedagogical focus and authorship by teachers and students. Thus, more studies that can meet the needs still in force are necessary, focusing on methods of utilization of technological resources in the network that enhance students' learning and development.

El estudio de una revisión sistemática sobre el impacto de las acciones de formación no el uso de tecnologías en las prácticas docentes. Una muestra de los resultados científicos completos indexados en la base SciELO, publicado entre los años de 2003 y 2016. No hay sentido de buscar la comprensión crítica de las comunicaciones y de sus significaciones, se ha hecho un análisis de contenido temático, segundo Bardin (2002). A revisión de resultados de los resultados de los impactos deseados en relación con el uso de tecnologías en las prácticas docentes con el enfoque pedagógico y la autoría por parte de los profesores y los estudiantes. Así, los estudios que se realizan en el ámbito de las necesidades aún no vigentes, se centran en las prácticas de la utilización de los recursos tecnológicos en la definición de la posibilidad de aprendizaje y el desarrollo de los estudiantes.

Cultura organizacional: revisão sistemática da literatura / Organizational culture: a systematic literature review / Cultura organizacional: una revisión sistemática de la literatura

A produção de conhecimento em cultura organizacional é caracterizada pela multiplicidade de perspectivas conceituais, teóricas e metodológicas, o que gera a necessidade de análise dos principais temas relacionados ao fenômeno nas organizações. Por isso, realizou-se uma revisão sistemática da literatura sobre cultura organizacional em junho de 2015, com a seleção de artigos publicados entre 2002 e 2015 nas bases SciELO e PePSIC. A análise dos dados de 53 estudos permitiu a identificação de seis categorias analíticas relacionadas às temáticas do fenômeno: (a) mudanças no modelo de gestão, (b) relações de poder, (c) gestão de pessoas, (d) estudos de culturas organizacionais específicas, (e) teste ou proposição de abordagem teórica e (f) instrumentos de medida. Os resultados demonstraram dispersão de questões e de temas, não apontando uma agenda convergente de pesquisas. Um consenso entre os pesquisadores é a necessidade de avaliar aspectos culturais das organizações antes de qualquer proposta de pesquisa ou de intervenção.

The production of knowledge on organizational culture has been characterized by a multiplicity of conceptual, theoretical, and methodological perspectives, which raises the need to analyze the major themes related to this phenomenon in organizations. Thus, a systematic review of the literature on organizational culture was carried out. The survey took place in June 2015, with the selection of 53 articles published between 2002 and 2015 indexed in the SciELO and PePSIC bases. The analysis of the data allowed us to identify 6 analytical categories related to the phenomenon: changes in the management model, power relations, management of people, studies of specific organizational cultures, test or theoretical approach proposition, and measurement instruments. The results show a dispersion of subjects and themes without pointing towards a converging research agenda. A consensus among researchers is the need for assessing cultural aspects of organizations prior to proposing any research project or intervention.

La producción de conocimiento en la cultura organizacional se caracteriza por la multiplicidad de perspectivas conceptuales, teóricas y metodológicas, lo que genera la necesidad de un análisis de los principales temas relacionados con el fenómeno en las organizaciones. Por ello, se llevó a cabo una revisión sistemática de la literatura sobre cultura organizacional en junio de 2015 con la selección de 53 artículos publicados entre 2002 y 2015 en las bases SciELO y PePSIC. El análisis de los datos permitió la identificación de 6 categorías de analíticas relacionadas con los temas del fenómeno: cambios en los modelos de gestión, las relaciones de poder, gestión de personas, estudios de culturas organizacionales específicas, prueba o proposición de abordaje teórica y instrumentos de medición. Los resultados muestran una dispersión de cuestiones y temas, no señalando una agenda de investigación convergente. Un consenso entre los investigadores es la necesidad de evaluar los aspectos culturales de las organizaciones antes de cualquier propuesta de investigación o intervención.

Occurrence and characterization of insect galls in the Floresta Nacional de Silvânia, Brazil

In the present paper we investigated the insect gall distribution along savanna and forest sites in the Floresta Nacional de Silvânia, Goiás, Brazil. The insect gall fauna was surveyed bi-monthly between December 2009 and June 2010. In total we found 186 insect gall morphotypes, distributed on 35 botanical families and 61 plant species. Ninety-nine insect gall morphotypes were recorded in the forest and 87 in the savanna. Gall-inducing insects belonged to Coleoptera, Diptera, Hemiptera, Lepidoptera and Thysanoptera, with highlight to Cecidomyiidae (Diptera) that induced 34.1% of the gall morphotypes. Parasitoids and/or inquilines were recorded in 38 morphotypes, mainly from the families Eulophidae, Eurytomidae and Torymidae (Hymenoptera). Fabaceae was the botanical family with the greatest richness of galls, followed by Asteraceae and Sapindaceae, being Protium (Burseraceae), Siparuna (Siparunaceae) and Serjania (Sapindaceae) the main host genera. This is the first systematic survey of insect galls realized in the Flona-Silvânia, which result in six plant species are recorded for the first time in Brazil as host of insect galls.

No presente estudo foi investigada a distribuição de galhas de insetos ao longo de áreas de cerrado e floresta na loresta Nacional de Silvânia, Goiás, Brasil. A fauna de insetos galhadores foi amostrada bimensalmente entre dezembro de 2009 e junho de 2010. No total foram encontrados 186 morfotipos de galhas de insetos, distribuídos em 35 famílias botânicas e 61 espécies de plantas. Foram registrados 99 morfotipos de galhas de insetos na floresta e 87 no cerrado. Os insetos galhadores pertenceram à Coleoptera, Diptera, Hemiptera, Lepidoptera e Thysanoptera, com destaque para Cecidomyiidae (Diptera) que induziu 34,1% dos morfotipos de galhas. Parasitoides e/ou inquilinos foram registrados em 38 morfotipos de galhas, principalmente das famílias Eulophidae, Eurytomidae e Torymidae (Hymenoptera). Fabaceae foi a família botânica com maior riqueza de galhas, seguida por Asteraceae e Sapindaceae, enquanto Protium (Burseraceae), Siparuna(Siparunaceae) e Serjania(Sapindaceae) foram os principais gêneros hospedeiros. Este é o primeiro levantamento sistemático de galhas de insetos na Flona-Silvânia, o que resultou em seis espécies de plantas sendo registradas pela primeira vez no Brasil como hospedeiras de galhas de insetos.