Serosurvey of anti- Leptospira sp. and anti- Toxoplasma gondii antibodies in capybaras and collared and white-lipped peccaries.

INTRODUCTION:: Brazilian native species are reemerging as increasingly free-ranging populations. METHODS:: Sera from 31 capybaras (Hydrochoerus hydrochaeris) and 28 peccaries (Pecari tajacu and Tayassu pecari) were tested for anti-Leptospira and anti-Toxoplasma gondii antibodies using microscopic seroagglutination test. RESULTS:: Nineteen percent of free-ranging and 10.0% of captive capybaras, along with 31.8% of collared peccaries, were seropositive for T. gondii. None was seropositive for Leptospira sp. CONCLUSIONS:: The present findings indicated low risk of disease, particularly among capybaras and white-lipped peccaries; however, active surveillance programs are important for monitoring wildlife health and public health once they are in public parks around cities.

Serosurvey of anti- Leptospira sp. and anti- Toxoplasma gondii antibodies in capybaras and collared and white-lipped peccaries

Abstract INTRODUCTION: Brazilian native species are reemerging as increasingly free-ranging populations. METHODS: Sera from 31 capybaras (Hydrochoerus hydrochaeris) and 28 peccaries (Pecari tajacu and Tayassu pecari) were tested for anti-Leptospira and anti-Toxoplasma gondii antibodies using microscopic seroagglutination test. RESULTS: Nineteen percent of free-ranging and 10.0% of captive capybaras, along with 31.8% of collared peccaries, were seropositive for T. gondii. None was seropositive for Leptospira sp. CONCLUSIONS: The present findings indicated low risk of disease, particularly among capybaras and white-lipped peccaries; however, active surveillance programs are important for monitoring wildlife health and public health once they are in public parks around cities.

PCR and qPCR for detection of Porcine Circovirus type 2 (PCV2) in captive white-lipped (Tayassu pecari) and collared (Tayassu tajacu) peccaries from Southern Brazil / PCR e qPCR para detecção de Circovírus Suíno tipo 2 (PCV2) em catetos (Pecari tajacu) e queixadas (Tayassu pecari) de cativeiro do Sul do Brasil

Porcine circovirus type 2 (PCV2) is an emergent virus found in commercial pig farms and may cause clinical or subclinical infection. The collared peccary (Pecari tajacu) and white-lipped peccary (Tayassu pecari) may also be infected by PCV2. Accordingly, the aim of this study was to identify PCV2 in whole blood samples of captive peccaries (16 collared and 6 white-lipped) by conventional and quantitative PCR (qPCR). Although the housekeeping gene (c-myc) DNA was successfully amplified, all 22 peccaries tested were negative for PCV2 by both molecular methods. In conclusion, although PCV2 may be endemic in free ranging wild pigs of Central and Northern Brazil, lack of serological and molecular PCV2 evidence (in whole blood) of both captive and free-range wild pigs may indicate low risk of disease in Southern Brazil.(AU)

O circovírus suíno tipo 2 (PCV2) é um vírus emergente encontrado em granjas comerciais de suínos que pode causar infecção clínica ou subclínica. Os catetos (Pecari tajacu) e as queixadas (Tayassu pecari) também podem se infectar com PCV2. Deste modo, o objetivo deste estudo foi identificar o PCV2 em amostras de sangue total de pecaris de cativeiro (16 catetos e seis queixadas) por PCR convencional e quantitativo (qPCR). Embora o gene constitutivo (c-myc) tenha sido amplificado com sucesso, todas as 22 amostras de pecaris avaliadas foram negativas para PCV2 em ambos os métodos moleculares. Em conclusão, embora o PCV2 seja endêmico em suínos selvagens de vida livre do Centro e Norte do Brasil, a ausência de evidência sorológica e molecular de PCV2 (em sangue total) de ambos pecaris de cativeiro e de vida livre no Sul do Brasil podem indicar baixo risco de doença no Sul do Brasil.(AU)

PCR and qPCR for detection of Porcine Circovirus type 2 (PCV2) in captive white-lipped (Tayassu pecari) and collared (Tayassu tajacu) peccaries from Southern Brazil / PCR e qPCR para detecção de Circovírus Suíno tipo 2 (PCV2) em catetos (Pecari tajacu) e queixadas (Tayassu pecari) de cativeiro do Sul do Brasil

Porcine circovirus type 2 (PCV2) is an emergent virus found in commercial pig farms and may cause clinical or subclinical infection. The collared peccary (Pecari tajacu) and white-lipped peccary (Tayassu pecari) may also be infected by PCV2. Accordingly, the aim of this study was to identify PCV2 in whole blood samples of captive peccaries (16 collared and 6 white-lipped) by conventional and quantitative PCR (qPCR). Although the housekeeping gene (c-myc) DNA was successfully amplified, all 22 peccaries tested were negative for PCV2 by both molecular methods. In conclusion, although PCV2 may be endemic in free ranging wild pigs of Central and Northern Brazil, lack of serological and molecular PCV2 evidence (in whole blood) of both captive and free-range wild pigs may indicate low risk of disease in Southern Brazil.

O circovírus suíno tipo 2 (PCV2) é um vírus emergente encontrado em granjas comerciais de suínos que pode causar infecção clínica ou subclínica. Os catetos (Pecari tajacu) e as queixadas (Tayassu pecari) também podem se infectar com PCV2. Deste modo, o objetivo deste estudo foi identificar o PCV2 em amostras de sangue total de pecaris de cativeiro (16 catetos e seis queixadas) por PCR convencional e quantitativo (qPCR). Embora o gene constitutivo (c-myc) tenha sido amplificado com sucesso, todas as 22 amostras de pecaris avaliadas foram negativas para PCV2 em ambos os métodos moleculares. Em conclusão, embora o PCV2 seja endêmico em suínos selvagens de vida livre do Centro e Norte do Brasil, a ausência de evidência sorológica e molecular de PCV2 (em sangue total) de ambos pecaris de cativeiro e de vida livre no Sul do Brasil podem indicar baixo risco de doença no Sul do Brasil.