Application on infrared spectroscopy for the analysis of total phenolic compounds in fruits.

Recent studies have demonstrated the metabolic benefits of phenolic compounds on human health. However, traditional analytical methods used for quantification of total phenolic compounds are time-consuming, laborious, require a high volume of reagents, mostly toxic substances, and involve several steps that can result in systematic and instrumental errors. Spectroscopic techniques have been used as alternatives to these methods for the determination of bioactive compounds directly in the food matrix by minimal sample preparation, without using toxic reagents. Therefore, this overview presents the advantages of nondestructive methods focusing on infrared spectroscopy (IR), for the quantification of total phenolic compounds in fruits. In addition, the main difficulties in applying these spectroscopic techniques are presented, as well as a comparison between the quantification of total phenolic compounds by traditional and IR methods. This review concludes by focusing on model building, highlighting that IR data are mainly processed using the partial least-squares (PLS) regression method to predict total phenolic content. The development of portable and inexpensive IR instruments, combined with multivariate data processing, could give to the consumers a straightforward technology to evaluate the total phenolic content of fruits prior to purchase.

Vicilin from Anadenanthera colubrina Seeds: An alternative tool to combat Callosobruchus maculatus.

Vicilins are seed proteins, and they constitute 70-80% of the total protein in leguminous seeds; with amolecular mass between 150 and 190 kDa, they are composed of subunits without disulfide bridges, with high affinity for chitin-binding. They are also associated with seed defense against insect pests. The chitin-binding vicilin from Anadenanthera colubrina seeds was purified by ammonium sulfate, followed by affinity chromatography on a chitin column, molecular exclusion on Superdex 75 Tricorn in FPLC system and Phenomenex C8 chromatography in HPLC system. The A. colubrina vicilin, named AcV, is a tetrameric glycoprotein composed of 1.55% carbohydrates and molecular weight determined by SDS-PAGE, consisting of 70, 73, 43 and 41 kDa. The AcV homogeneity was confirmed in native PAGE, where it was observed to be a unique band with slow mobility in this gel, with approximately 230 kDa. AcV added to the Callosobruchus maculatus diet in the bioassays resulted in a strong effect on adult emergence (ED50 of 0.096%), and in larvae caused a marked reduction in mass (WD50 of 0.32%) and lethality (LD50 of 0.33%) (w:w). The digestibility of AcV was evaluated in vitro with the digestive enzymes of larvae of C. maculatus of fourth instar, showing major fragments of 10 and 30 kDa. AcV showed reactivity against the anti-EvV antibody from Erythrina velutina vicilin. The deleterious effects of AcV are likely to be associated with the chitin-binding fragments generated by proteolysis in the bruchid gut, similarly to that found for vicilins from other leguminous plant species, Enterolobium contortisiliquum and Vigna unguiculata. AcV might be a candidate protein for a possible bioinsecticidal control of the bruchid weevil, C. maculatus.

Immunohistochemical diagnosis of tenacibaculosis in paraffin-embedded tissues of Senegalese sole Solea senegalensis Kaup, 1858.

A sensitive and specific immunohistochemical technique was developed to improve the diagnosis of tenacibaculosis and to better understand its pathogenesis. Senegalese sole Solea senegalensis Kaup, 1858 were inoculated subcutaneously with a bacterial suspension of Tenacibaculum maritimum, and samples were taken at different hours post-inoculation. Sections from different organs were used as positive controls. In addition, a total of 128 field samples from different organs collected from tenacibaculosis outbreaks were used. Tenacibaculum maritimum antigens were detected in several organs of experimentally infected Senegalese sole and in at least one of the tissues from fish suffering from natural tenacibaculosis previously confirmed by culture and PCR-based methods. In fish collected during outbreaks, a strong positive reaction was detected in ulcerative skin areas. Moreover, bacterial antigen was identified inside scale pockets and in sites of the skin with mild lesion. In kidney and spleen, evident immunostaining of bacterial antigen was detected in both naturally and experimentally infected fish. Besides, the presence of T. maritimum in the intestinal tract without associated histological changes suggests that this organ may act as a reservoir for T. maritimum. The results of this study confirm the usefulness of IHC for the diagnosis of tenacibaculosis in paraffin-embedded tissues.

Morphopathological features of a severe ulcerative disease outbreak associated with Tenacibaculum maritimum in cultivated sole, Solea senegalensis (L.).

This study describes morphopathologic changes in naturally infected farmed Senegalese sole affected by tenacibaculosis caused by Tenacibaculum maritimum. Macroscopic observation, in addition to light microscopy and scanning electron microscopy, was used to study the lesions. Main lesions were characterized by complete loss of epidermis and dermis, as well as extensive necrosis of muscle layers. Mild-to-moderate inflammatory response with the presence of macrophages was noted around hyaline degenerated muscle cells. Gram-negative filamentous bacteria could be detected only at the dermis. Under scanning electron microscopy, filamentous bacteria located over the scales without epithelium could be observed. These findings together with the isolation and PCR detection of the bacteria in kidney and skin tissues suggest that once the bacteria reach the dermis, probably through eroded epidermis, they are able to proliferate and produce enzymes that are responsible for the damage in the underlying tissues.

Assessment of work ability of health professionals in the mobile emergency unit.

Ergonomics is the study of a workplace and the worker. Its aim is to better adapt the workplace to man by preserving the body for short and long term work. This helps to adjust and improve functionality, thus preserving the body for short and long term work. It was through the observation of SAMU's (Mobile Emergency Unit) professional's helpers that the interest to evaluate these individuals arose. In addition, the aim of this research is to investigate the work ability of health professionals that work for SAMU/JP. The population was composed of 97 health professionals who currently work for SAMU/JP. A sociodemographic questionnaire was used as data collection instrument and it was validated by the index of the Work Ability (WAI). The research took place in 2010, in the headquarters of SAMU, in the city of João Pessoa, state of Paraíba - Brazil. The data analysis was carried out by simple descriptive statistics followed by comparison of the results with the pertinent literature. The quantity of daily sleeping hours, the levels of satisfaction in the job and the number of diagnosed diseases were among the most worrying factors. In spite of this, the health professionals obtained a work ability average considered to be "good".

Relationship between exposure to cement and the incidence of respiratory diseases in children: a preventive perspective.

Respiratory disease in childhood is a factor of concern to health professionals, resulting in a primary determinant of lung function in adulthood. Studies indicate that people placed in areas with established industries, are exposed to pollutants from many industrial processes, thus increasing the risk of diseases. The aim was to examine the connection between exposure to cement and the incidence of respiratory diseases in the Jardins Community. It was a field study, descriptive, conducted in a community in the city of Cabedelo- PB, and result in a qualitative-quantitative approach. The sample comprised 51 children aged between 1 to 12 years who have symptoms or diagnosis of respiratory disease. The survey data was obtained by applying a semistructured interview, and analysed using chi-square, MacNemar and Mann-Whitney tests for statistical analysis. A high number of complaints about environmental pollution, small houses and subdued, lack of paving and sanitation, a high frequency of respiratory symptoms and especially the occurrence of the onset of symptoms after arrival in the community were found. There is a correlation between environmental exposure and the incidence of respiratory diseases in children; therefore the community requires specific actions to reduce the exposure.

Fatty acid analysis as a chemotaxonomic tool for taxonomic and epidemiological characterization of four fish pathogenic Tenacibaculum species.

AIMS: In this work, fatty acid content and profiles were analysed in order to differentiate the species Tenacibaculum maritimum, Tenacibaculum gallaicum, Tenacibaculum discolor and Tenacibaculum ovolyticum that are pathogenic for cultured marine fish and to assess the potential of fatty acid profiles as a tool for epizootiological typing. METHODS AND RESULTS: The fatty acid methylesters (FAMEs) were extracted from cells grown on marine agar for 48 h at 25 degrees C and were prepared and analysed according to the standard protocol of the MIDI/Hewlett Packard Microbial Identification System. The cellular fatty acid profiles of Tenacibaculum strains tested were characterized by the presence of large amounts of branched (36.1-40.2%) and hydroxylated (29.6-31.7%) fatty acids. The FAME products from the four species significantly (P < 0.05) differed in the content of iso-C(15:0)3-OH, iso-C(16:0)3-OH, iso-C(15:1)G, summed feature 3 (a component that contains C(16:1)omega7c and/or iso-C(15:0) 2-OH), iso-C(16:0), C(17:1)omega6c, C(15:0)3-OH, iso-C(17:0)3-OH. CONCLUSIONS: Results of present study demonstrated the existence of differences in the fatty acids content between the T. maritimum isolates from different marine fish/geographical origin and between strains of T. maritimum, T. discolor, T. gallaicum and T. ovolyticum. SIGNIFICANCE AND IMPACT OF THE STUDY: Profiling of fatty acids may be a useful tool to distinguish T. maritimum from other Tenacibaculum species pathogenic for fish as well as for epizootiological differentiation of T. maritimum isolates.

Hepatopatía tóxica por amoxicilina/clavulánico / No disponible

Aproximadamente unos 1.000 medicamentos se asocian con lesión citotóxica hepática y/o colestasis. Larelación de causalidad entre la administración de amoxicilina/clavulánico y un cierto tipo de hepatitiscolestásica parece bien establecida.En un corto periodo de tiempo hemos diagnosticado 2 casos de hepatitis colestásica por oxicilina /clavulánico. Su conocimiento es importante a que antecedentes de ictericia colestásica y/o disfunción hepática ociada a este medicamento constituyen una contraindicación de utilización

Approximately 1000 drugs are associated with cytotoxic liver lesion and/or cholestasis. The causal relationshipbetween the administration of amoxicillin-clavulanic acid and a certain type of cholestatic hepatitisappears to be well established.In a short period of time, we have diagnosed two cases of cholestatic hepatitis induced by amoxicillin-clavulanic acid. It is important to be a familiar with this relationship since a history of cholestatic jaundiceand/or liver dysfunction associated with this drug constitutes a contraindication for its use

Efficacy of furunculosis vaccines in turbot, Scophthalmus maximus (L.): evaluation of immersion, oral and injection delivery.

The commercial furunculosis vaccine Aquavac Furovac 5 and an autogenous vaccine, based on the challenge strain, induced immune protection in turbot, Scophthalmus maximus (L.), as shown in challenge tests 120 days post-immunization by injection (relative percentage of survival, RPS = 72-99%). This protective effect lasted for at least 6 months post-immunization at appreciable levels (RPS = 50-52%). Neither the autogenous vaccine nor the commercial vaccine was able to induce significant levels of protection against Aeromonas salmonicida in turbot when administered by immersion. Antibody levels were high or moderate in fish vaccinated by injection with the different vaccines and very low in fish vaccinated by immersion. The field results show that delivering an oral boost after the primary vaccination by injection did not enhance protection of turbot against furunculosis and that water-based (autogenous vaccine) and oil adjuvanted (Alpha Ject 1200) vaccines administered by injection conferred similar levels of protection (RPS > 80%) in turbot.

Evaluation of the AQUARAPID-Va, AQUAEIA-Va and dot-blot assays for the detection of Vibrio anguillarum in fish tissues.

The comparative accuracy of the serological assays AQUARAPID-Va, AQUAEIA-Va (BIONOR AS), and dot-blot for a rapid diagnosis of vibriosis in fish was evaluated. Twenty-one Vibrio anguillarum strains, representative of pathogenic and environmental serotypes, and 13 strains of other fish pathogenic bacteria were used to assess the sensitivity and specificity of the detection methods. The serological assays tested detected all the strains of V. anguillarum serotypes O1 and O2. The dot-blot assay was the most specific and sensitive method, detecting almost all isolates from serotypes O1, O2 and O3, with an average sensitivity of 1 x 10(6) bacteria g(-1) of fish tissue. The AQUARAPID-Va and the AQUAEIA-Va systems were able to detect 5 x 10(6) and 5 x 10(7) bacteria g(-1) of fish tissue, respectively. The simplicity, effectiveness and speed of the AQUARAPID-Va system confirmed this method as the most suitable serological test for the detection of V. anguillarum in field analysis and small-scale laboratory studies.

Detection of Flexibacter maritimus in fish tissue using nested PCR amplification.

A nested polymerase chain reaction (PCR) system was developed for the detection of Flexibacter maritimus from fish tissue. The total procedure for the diagnosis of marine flexibacteriosis, from the point of DNA extraction to the electrophoretic analysis, can be performed in < 4 h. This was achieved by the combination of a short thermal cycling programme with a rapid DNA extraction procedure. The assay was extremely sensitive, capable of detecting as few as 75 cfu mg(-1) fish tissue. The accuracy of the nested PCR was confirmed under field conditions using tissue samples recovered during 1993-2002 from fish suffering marine flexibacteriosis. The nested PCR method proved to be efficient for the rapid and sensitive detection of F. maritimus from fish tissues and can be used for routine diagnosis of the disease caused by this pathogen.

A proposed serotyping system for Flavobacterium psychrophilum.

AIMS: To develop a common serological system for rapid and routine identification of the fish pathogen Flavobacterium psychrophilum. METHODS: Thirty-four isolates of Fl. psychrophilum from different fish species and different geographical areas were typed using a slide agglutination test and an enzyme-linked immunosorbent assay (ELISA). RESULTS: Seven host-dependent serovars (1: salmon; 2: trout; 3: trout; 4: eel; 5: carp; 6: tench; 7: ayu) were found. Serovar 2 was divided into two antigenic subgroups (2a and 2b). The results achieved by both slide agglutination and ELISA methods were totally consistent with each other. Although both techniques proved to be simple to carry out and useful, only the ELISA allowed identification of Fl. psychrophilum serovars using unabsorbed antiserum and whole-cells as antigens. SIGNIFICANCE AND IMPACT OF THE STUDY: This paper proposes a harmonized scheme for serological identification of Fl. psychrophilum to be used for diagnostic and seroepidemiological studies of the diseases it causes.