Evaluation of proteome dynamics: Implications for statistical confidence in mass spectrometric determination.

Single-cell proteomics is currently far less productive than other approaches. Still, the proteomic community is having trouble adapting to the limitation of having to examine fewer cells than they would like. Studies on a small number of cells should be carefully planned to maximize the chances of success in this situation. This study aims to determine how sample size and measurement speed (slope)/variation affect the accuracy of a protein proteome mass spectrometric determination. The determination accuracy was shown to increase, and the false positive rate was shown to decrease as the sample size increased from 7 to 100 cells and the measurement slope/variation (S/V) ratio increased from 1 to 6. Furthermore, it was discovered that the number of cells in the sample increased the accuracy of this estimate. Thus, for 100 cells, the measurement S/V ratio was typically estimated to be very close to the real-world value, with a standard deviation of 0.35. For sample sizes from 7 to 100 cells, this accuracy was seen when calculating the measurement S/V ratio. The findings can help researchers plan experiments for mass spectroscopic protein proteome determination and other research purposes.

The use of antigens derived from Bacillus thuringiensis bacteria for further differentiation.

This study is devoted to studying Bacillus thuringiensis antigens and their insecticide activity as critical features in bacterial differentiation. Indeed, 190 samples were examined for flagellar antigenicity as well as the insecticidal activity exhibited. From a serological perspective, 122 isolates (64.2 %) were attributed to 8 H-serogroups, including 3 non-typeable and 65 unverified. The dominant serotype was H3abc (82 % frequency); H6 was less frequent (8.5 %). The other 6 serotypes accounted for a low frequency of occurrence (up to 1.5 %). Of the 190 isolates tested, 125 (65.8 %) formed bipyramidal, and 63 (33.2 %) represented spherical inclusions. All H3abc isolates contained bipyramidal inclusions. The same applied to H8ab and H7 isolates. Insecticide activity was noted in 70.1 % of the population. In general, 128 samples were toxic to both species (Bombyx mori, Aedes sp.). Another 3 samples were toxic only to B. mori, and 2 for Aedes sp. Among the samples exhibiting toxicity to both species, 97.6 % belonged to bipyramidal paraspore inclusions (H3abc). All H7 samples were toxic to two insect species. Monotoxic B. thuringiensis against Aedes sp. were found only among organisms producing spherical parasporal inclusions in the cell. Examples of such microorganisms include an isolate of the H4ab/43 serotype.

Effect of Domain Structure and Dielectric Interlayer on Switching Speed of Ferroelectric Hf0.5Zr0.5O2 Film.

The nanosecond speed of information writing and reading is recognized as one of the main advantages of next-generation non-volatile ferroelectric memory based on hafnium oxide thin films. However, the kinetics of polarization switching in this material have a complex nature, and despite the high speed of internal switching, the real speed can deteriorate significantly due to various external reasons. In this work, we reveal that the domain structure and the dielectric layer formed at the electrode interface contribute significantly to the polarization switching speed of 10 nm thick Hf0.5Zr0.5O2 (HZO) film. The mechanism of speed degradation is related to the generation of charged defects in the film which accompany the formation of the interfacial dielectric layer during oxidization of the electrode. Such defects are pinning centers that prevent domain propagation upon polarization switching. To clarify this issue, we fabricate two types of similar W/HZO/TiN capacitor structures, differing only in the thickness of the electrode interlayer, and compare their ferroelectric (including local ferroelectric), dielectric, structural (including microstructural), chemical, and morphological properties, which are comprehensively investigated using several advanced techniques, in particular, hard X-ray photoelectron spectroscopy, high-resolution transmission electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, and electron beam induced current technique.

Rigid class scheduling and its value for online learning in higher education.

The spread of coronavirus infection brings changes to all spheres of activity, including education, which is increasingly moving to a distance learning format. The ultimate goal of this study was to investigate the effectiveness of the recommendations for developing a rigid class scheduling method in the framework of online learning using advanced approaches updating traditional learning mechanisms. Overall, this research was conducted among 226 students and 54 teachers representing Moscow Aviation Institute and Kazan Federal University. Research methodology basis was represented by a sociological survey, which allowed identifying the problems of implementation of online learning from the students' standpoint. The collected outcomes showed that for 35% of students, one of the main problems in distance learning was the lack of self-discipline, which can be further adjusted by a clearly established schedule of classes (37% of respondents) and tests (29% of respondents). At the same time, the collected data showed that the learning process was most efficient in subgroups 1 (0.5), 2 (0.3), and 5 (0.45). Recommendations provided for further distance education improvement, first of all, related to schedule formation issues. More precisely, the paper emphasized the need for time constraints, even distribution of subjects, use of special applications, designation of dates for knowledge checking, and inclusion of additional learning materials. The findings and results of this work are believed to be of scientific value for the methodological departments of educational institutions responsible for curricula preparation as they are expected to significantly contribute to educational process advancement.

Viral rhodopsins 1 are an unique family of light-gated cation channels.

Phytoplankton is the base of the marine food chain as well as oxygen and carbon cycles and thus plays a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms and regulate the phytoplankton dynamics encompass genes of rhodopsins of two distinct families. Here, we present a functional and structural characterization of two proteins of viral rhodopsin group 1, OLPVR1 and VirChR1. Functional analysis of VirChR1 shows that it is a highly selective, Na+/K+-conducting channel and, in contrast to known cation channelrhodopsins, it is impermeable to Ca2+ ions. We show that, upon illumination, VirChR1 is able to drive neural firing. The 1.4 Å resolution structure of OLPVR1 reveals remarkable differences from the known channelrhodopsins and a unique ion-conducting pathway. Thus, viral rhodopsins 1 represent a unique, large group of light-gated channels (viral channelrhodopsins, VirChR1s). In nature, VirChR1s likely mediate phototaxis of algae enhancing the host anabolic processes to support virus reproduction, and therefore, might play a major role in global phytoplankton dynamics. Moreover, VirChR1s have unique potential for optogenetics as they lack possibly noxious Ca2+ permeability.