RESUMEN
Liver and muscle samples from 7 species of aquatic and terrestrial predatory birds from Flanders (Belgium) were analysed for polychlorinated biphenyls (PCBs), polybrominated diphenylethers (PBDEs) and organochlorine pesticides (OCPs). PCBs were the predominant compounds in our samples, which showed highest concentrations in the liver of barn owl (Tyto alba) and sparrowhawk (Accipiter nisus) (up to 1000 microg/g lipid weight). PBDEs could be determined in most samples at lower concentrations than PCBs (up to 64 microg/g lipid in sparrowhawk liver). Sparrowhawks had the highest levels of hexachlorobenzene, DDTs and PBDEs. In contrast, kestrels (Falco tinnunculus) had relatively low levels of most measured organochlorines. BDE 47 was the most abundant congener in heron (Ardea cinerea) and grebe (Podiceps cristatus), while BDE 47, 99 and 153 were equally important in the terrestrial species. BDE 183 and BDE 209 were only measured in the terrestrial birds. These results indicate that terrestrial birds may be more exposed to higher brominated BDE congeners than aquatic species.
Asunto(s)
Aves , Retardadores de Llama/análisis , Hidrocarburos Clorados/análisis , Plaguicidas/análisis , Animales , Bélgica , Exposición a Riesgos Ambientales , Monitoreo del Ambiente/métodos , Peces , Retardadores de Llama/toxicidad , Cadena Alimentaria , Éteres Difenilos Halogenados , Hidrocarburos Clorados/toxicidad , Hígado/química , Músculo Esquelético/química , Plaguicidas/toxicidad , Éteres Fenílicos/análisis , Bifenilos Polibrominados/análisis , Bifenilos Polibrominados/toxicidad , Bifenilos Policlorados/análisis , Contaminantes del Suelo/análisis , Especificidad de la Especie , Distribución Tisular , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
The effects of polychlorinated biphenyls (PCB 77, PCB 153, and the mixture Aroclor 1242) on circulating and intracellular thyroid hormone (TH) levels were studied during chicken embryonic development. We observed no influences of PCB 153 on TH availability. Aroclor 1242 caused a transient increase in the T(3) level in the cerebellum at day 16. Clear effects were only seen with PCB 77 around the period of hatching: a severely reduced TH peak, which normally coincides with the stage of internal pipping, and a considerable delay in the moment of hatching.
Asunto(s)
Embrión de Pollo/metabolismo , Bifenilos Policlorados/metabolismo , Hormonas Tiroideas/metabolismo , Animales , Animales Recién Nacidos , Embrión de Pollo/efectos de los fármacos , Embrión de Pollo/embriología , Bifenilos Policlorados/farmacología , Factores de TiempoRESUMEN
A rapid and simple analytical method for the determination of organochlorines, such as polychlorinated biphenyls (PCBs) and selected organochlorinated pesticides (OCPs) and organobromines, such as polybrominated diphenyl ethers (PBDEs), in sediment samples was optimised using CRM 536 (PCBs in freshwater sediment). The method involved a hot Soxhlet extraction that reduced the extraction time to 2 h. Elemental sulphur, which is present in sediments and may interfere during the analysis, was removed by means of copper powder added to the sediment during extraction and into the clean-up cartridge. The analysis of PCBs and OCPs was accomplished by gas chromatography with electron capture or mass spectrometric detection. Similar quantitative results for PCB congeners in CRM 536 were obtained using a 50-m capillary column and a 10-m narrow bore column suited for fast analysis. The analysis of PBDEs was done by mass spectrometry in negative chemical ionisation mode. Concentrations of organic pollutants in two sediment cores (approximately 50 cm depth) from the Scheldt river (south of Antwerp, Belgium) showed a relative steady state for PCBs and DDTs, with a slight decrease in the top layers, suggesting a slight decline in their concentrations due to restrictions in their usage. On the contrary, PBDEs were showing an increase in their concentrations in the top layers (up to 270 and 8400 ng/g dry weight for sum of tri- to hexa-BDE congeners and for BDE 209, respectively). This suggests an increasing trend in the concentrations of PBDEs in the Belgian environment.
Asunto(s)
Contaminantes Ambientales/análisis , Plaguicidas/análisis , Bifenilos Polibrominados/análisis , Bifenilos Policlorados/análisis , Bélgica , Monitoreo del Ambiente , Éteres , Sedimentos Geológicos/química , RíosRESUMEN
During one to three consecutive periods of 2 weeks, broiler chickens (n = 108) received test dies to which different amount of PCBs (7 congeners) were added. The relationship between exposure time and accumulation of individual congeners in different chicken tissues, such as breast, thigh and abdominal fat tissue, was observed. In all tissues, the vast majority of the PCB accumulation occurred during the first 2 weeks of exposures. After that, PCB concentrations only increased in the abdominal fat tissue of the animals. The individual PCBs were distributed differently in the various tissues. While CBs 28, 118, 138, 153 and 180 accumulated in the chickens, CBs 52 and 101 were metabolized, but no methyl sulphone metabolites of these congeners could be detected. Our results provide information on the absorption, tissue distribution and biotransformation of the individual PCB congeners and confirm the structure-activity relationships for metabolism of PCBs in birds, which are different from those in fish or mammalian species.
Asunto(s)
Pollos/metabolismo , Bifenilos Policlorados/farmacocinética , Absorción , Análisis de Varianza , Alimentación Animal/análisis , Animales , Biotransformación/fisiología , Cromatografía de Gases , Contaminación de Alimentos/análisis , Bifenilos Policlorados/metabolismo , Factores de Tiempo , Distribución TisularRESUMEN
Concentrations of hexachlorobenzene (HCB), alpha-, beta- and gamma-hexachlorocyclohexane (HCH) isomers, 6 o,p'-and p,p'-isomers of DDT and 28 PCB congeners have been measured in eleven soil samples and one lichen collected on the Eastern coast of Antarctica from 5 Russian stations. For samples with low concentrations of PCBs (range 0.20-0.41 ng g(-1) dry weight) and pesticides (0.86-4.69 ng g(-1) and 0.11-1.22 ng g(-1) dry weight for HCHs and DDTs, respectively), atmospheric long-range transport from Africa, South America or Australia was suggested as the sole source of contamination. The profile of PCB congeners was dominated by the more volatile tri-, tetra- and penta-PCBs congeners, thus supporting long-range transport hypothesis. Four samples contained moderate levels of PCBs (range 1.98-6.94 ng g(-1) dry weight) and variable concentrations of pesticides (gamma-HCH, p,p'-DDT and o,p'-DDT being the main contaminants). For samples with high concentrations of PCBs (range 90.26-157.45 ng g(-1)) and high concentrations of pesticides, the presence of high molecular weight PCB congeners such as: 153, 180, 187, 170 etc, strongly suggest a local source (biotic) of PCBs rather than atmospheric transport. It is likely that on a local scale, biotic focussing of pollutants, due to bird activities (nesting and excrement) can cause high contamination levels and become more significant than contaminant input via abiotic pathways.
Asunto(s)
Contaminantes Ambientales/análisis , Insecticidas/análisis , Bifenilos Policlorados/análisis , Contaminantes del Suelo/análisis , Movimientos del Aire , Regiones Antárticas , Monitoreo del Ambiente , Líquenes/química , Federación de RusiaRESUMEN
The influence of dietary amounts of polychlorinated biphenyls (PCBs) was studied on performance, apparent PCB digestibility and PCB accumulation in broiler chickens that were maintained until 42 days of age. Dietary concentrations of supplemented PCBs, based on the sum of seven reference congeners, ranged from 0 to 12 ng/g, which was below the legal maximum of 200 ng PCBs/g fat in Belgian feeds. PCB ingestion did not significantly affect body weight and feed intake. Apparent PCB digestibility was not influenced by dietary levels of added fat varying between 4% and 8%, but was significantly higher in broilers fed diets containing added PCBs. Accumulation of PCBs in the fat fraction of abdominal adipose tissue and breast and thigh muscle greatly depended upon PCB intake. However, PCB contents in the various body fat fractions within the same animal differed, even within muscle tissues, indicating an unequal PCB distribution in body fats.
Asunto(s)
Pollos/metabolismo , Contaminantes Ambientales/farmacocinética , Contaminación de Alimentos , Alimentos/normas , Bifenilos Policlorados/farmacocinética , Tejido Adiposo/metabolismo , Alimentación Animal , Animales , Cadena Alimentaria , Absorción Intestinal , Músculos/metabolismo , Distribución TisularRESUMEN
Validated analytical procedures for the determination of 21 organochlorine pesticides have been applied to 74 water samples, 76 soil samples, and 160 vegetable samples from nine Sene-Gambian farms. Mean pesticide residue levels found were compared to the results of other studies. The main contaminants were DDTs in water (231.9 ng/L), in soil (71.4 ng/g), and in vegetables (5.03 ng/g). The distribution of pesticide residues in water and neighboring soils and the soil-plant transfer of these pesticides is briefly discussed. Different bioconcentration factors for sum HCHs, sum DDTs, and sum endosulfans obtained in this study allow us to confirm the complex processes already reported in the literature concerning the uptake and translocation of chemicals from soil to plant.
Asunto(s)
DDT/análisis , Hexaclorociclohexano/análisis , Insecticidas/análisis , Residuos de Plaguicidas/análisis , Contaminantes del Suelo/análisis , Agricultura , Ciudades , Monitoreo del Ambiente , Contaminación de Alimentos , Gambia , Senegal , Verduras , Abastecimiento de AguaRESUMEN
In this study we investigated the accumulation of organochlorine compounds (HCB, 3 HCH-isomers, p,p'-DDT and its metabolites and 18 PCB congeners) in the muscle and fat tissue of nestling great tits (Parus major) from four study sites located in an area with extensive environmental contamination. The concentration of p,p'-DDT, p,p'-DDD, alpha-, beta-, gamma-HCH, and PCB congeners 128 and 149 were below the limit of detection in all muscle and fat samples. In muscle tissue the concentrations of HCB and congeners 28, 52, 101, 110, and 194 were in more than 50% of the cases below detection limit and these data were excluded from statistical analysis. Sigma PCB in muscle tissue ranged from 461 to 1060 ng/g lipid weight and in fat from 776 to 1779 ng/g lipid weight. p,p'-DDE had concentrations ranging from 106 to 205 ng/g lipid weight in muscle and from 201 to 348 ng/g lipid weight in fat. HCB concentrations were very low, ranging from ND to 7.0 ng/g lipid weight in fat. We found significant differences among study sites in the concentration of Sigma PCB and of almost all individual congeners in both muscle and fat tissue. Concentrations of p,p'-DDE in muscle and fat of great tit nestlings tended to differ among sites. Principal Component Analysis (PCA) showed that the study sites with the highest Sigma PCB level had a different PCB profile than the two other sites. Our study illustrates that insectivorous passerines with a limited home range, such as the great tit, are suitable biomonitors for terrestrial organochlorine contamination.
Asunto(s)
Exposición a Riesgos Ambientales , Contaminantes Ambientales/farmacocinética , Insecticidas/farmacocinética , Bifenilos Policlorados/farmacocinética , Pájaros Cantores , Tejido Adiposo/química , Animales , Animales Recién Nacidos , Monitoreo del Ambiente , Contaminantes Ambientales/análisis , Insecticidas/análisis , Músculo Esquelético/química , Bifenilos Policlorados/análisis , Distribución TisularAsunto(s)
Insecticidas/farmacocinética , Bifenilos Policlorados/farmacocinética , Marsopas , Animales , Bélgica , Dieta , Monitoreo del Ambiente , Femenino , Peces , Cadena Alimentaria , Insecticidas/análisis , Hígado/química , Masculino , Mar del Norte , Bifenilos Policlorados/análisis , Distribución TisularRESUMEN
Polychlorinated biphenyls (PCBs) and organochlorine pesticides, such as hexachlorobenzene (HCB) and 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethylene (p,p'-DDE) are compounds widespread in the environment, highly lipophilic, and accumulate in biological systems. The newborn are exposed to these organochlorine compounds across the placenta and through breastfeeding. This study reports the levels of selected PCB congeners, p,p'-DDE and HCB in maternal blood and cord blood samples collected at delivery between November and December 1999 from 44 women living in the urban area of Antwerp, Belgium. Results show that all newborns contained detectable levels of PCBs, p,p'-DDE and HCB. The median concentration of PCBs was 450 pg/ml and ranged between 120 and 1580 pg/ml, while the median concentrations of HCB and p,p'-DDE were 70 and 490 pg/ml, respectively. Concentrations of PCBs and p,p'-DDE in cord blood (ng/ml) were positively associated with concentrations in maternal blood (ng/ml) (coefficients=0.74 and 0.92, P<0.05). We conclude that all investigated organochlorine compounds have an efficient transplacental transfer.
Asunto(s)
Lactancia Materna , Contaminantes Ambientales/farmacocinética , Sangre Fetal/química , Insecticidas/farmacocinética , Intercambio Materno-Fetal , Bifenilos Policlorados/farmacocinética , Adulto , Bélgica , Contaminantes Ambientales/análisis , Contaminantes Ambientales/sangre , Femenino , Humanos , Recién Nacido , Insecticidas/análisis , Insecticidas/sangre , Placenta , Bifenilos Policlorados/análisis , Bifenilos Policlorados/sangre , Embarazo , Población UrbanaRESUMEN
The core paper of this debate shows that persistent organic pollutant residues of the 12 chemicals targeted for a phase out under the Stockholm Convention are present in almost all categories of food in the US food supply. For dioxins, the study does not use measured data, but is based upon potential dioxin residues in selected food items. Polychlorinated biphenyls are not included in the study. In this paper we discuss selected data of polychlorinated biphenyl and dioxin concentrations in Belgian food. Some of these exposures are chronic, others are attributable to incidents. Both result in high body burdens in Belgium. The paper also compares the current concentrations in food with the recent standards launched by the EU for dioxins in food, and discusses whether these values adequately protect European citizens.
Asunto(s)
Dioxinas/análisis , Contaminación de Alimentos/análisis , Residuos de Plaguicidas/análisis , Bifenilos Policlorados/análisis , Bélgica , Contaminantes Ambientales/análisis , HumanosRESUMEN
In 1999, a campaign of the Flemish Ministry of Health, Belgium was set up to assess pollutant concentrations and related health effect biomarkers in humans living in two regions of Flanders. The study was called the 'Flemish Environment and Health Study' (FLEHS). One of the goals was to measure present concentrations of persistent organochlorine pollutants in a Flemish population and to compare values obtained from pooled and individual serum samples. Concentrations of selected organochlorine pesticides, polychlorinated biphenyls (PCB) and polychlorinated dibenzo-p-dioxins (PCDD) and furans (PCDF) were measured by gas chromatography-mass spectrometry. TEQ values were also assessed by Chemical-Activated LUciferase gene eXpression (CALUX) bioassay. The study population consisted of 200 women between 50 and 65 years living in two areas of Flanders, Belgium. Because of the large volumes serum needed for all measurements, the concentrations of organochlorines were measured in 47 pooled serum samples originating from these women. The concentrations of the indicator PCBs (359.8 ng/g fat) and organochlorine pesticides (hexachlorobenzene, p,p'-dichlorodiphenyldichloroethylene, p,p'-dichlorodiphenyl-trichloroethane, lindane and pentachlorophenol), were comparable to those found in other European countries. The concentrations of PCDD/PCDFs showed another picture. With a median value of 48 pg WHO-TEQ/g fat, the women had 2-fold higher levels than a comparable age group from Germany examined in 1996. The mean total WHO-TEQ including PCDD/F, non-ortho and mono-ortho PCBs was 72.7 pg WHO-TEQ/g fat, whereas the CALUX-TEQ mean value was only 35.0 pg TEQ/g fat. In order to assess the pooling procedure, indicator PCBs and CALUX-TEQs were measured in all 200 individuals that were integrated in the pools. The measured values were comparable to the pool results: 390.0 ng/g fat and 41.6 pg TEQ/g fat respectively. It was concluded that pooling of serum samples offers the possibility to measure exposure in the whole study population on a more cost-effective way. However, because of statistical power loss and no possibility of confounder adjustment, pooling is not the most effective way to study regional differences.
Asunto(s)
Benzofuranos/sangre , Exposición a Riesgos Ambientales , Contaminantes Ambientales/sangre , Insecticidas/sangre , Bifenilos Policlorados/sangre , Anciano , Biomarcadores/análisis , Dibenzofuranos Policlorados , Femenino , Cromatografía de Gases y Espectrometría de Masas , Humanos , Persona de Mediana Edad , Países Bajos , Valores de ReferenciaRESUMEN
The concentrations of organochlorine pesticides (OCPs) in fish, shrimps, cattle fat and human serum samples from the Sene-Gambian region were measured using validated analytical methodologies. The results obtained were compared with those of other existing African studies and with data from other developing countries. In fish samples, p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and HCB were detected with a frequency of 100%, whereas p,p'-dichlorodiphenyltrichloroethane (p,p'-DDT) was detected in eight, heptachlorepoxide in six and endosulfansulfate in five of the nine fish samples. Relatively low concentrations of OCPs were found in cattle and shrimp fat samples. p,p'-DDE and alpha-hexachlorocyclohexane (alpha-HCH) were the most frequently identified. The sum of HCHs ranged from undetected to 13.3 ng g-1 fat, the sum of DDTs from 11.1 to 199.2 ng g-1 fat and the sum of endosulfans from not detected to 49.7 ng g-1 fat in fish and shrimps. In serum samples, alpha-HCH, p,p'-DDE, o,p'-DDT and p,p'-DDT were detected in all 16 pooled serum samples, whereas endosulfansulfate, methoxychlor, mirex, heptachlorepoxide and endrin were detected in 15 samples with most of the concentrations below 10 ng mL-1. The concentrations of OCPs in human serum were given on a serum lipid and whole serum volume basis. The implications for the human diet of these OCP concentrations in serum were investigated by means of biomagnification factors related to the log Kow values of the targeted compounds. The current use of HCH mixtures was suggested to explain the unusually high alpha-HCH concentration. The distribution pattern of these OCPs in humans was also discussed and compared with that in other studies.
Asunto(s)
Contaminación de Alimentos , Hidrocarburos Clorados , Insecticidas/farmacocinética , Carne , Penaeidae/química , Mariscos , Contaminantes Químicos del Agua/farmacocinética , Tejido Adiposo/química , Adolescente , Adulto , Animales , Bovinos , Monitoreo del Ambiente , Femenino , Peces , Gambia , Humanos , Insecticidas/sangre , Masculino , Persona de Mediana Edad , Senegal , Distribución Tisular , Contaminantes Químicos del Agua/sangreRESUMEN
BACKGROUND AND OBJECTIVES: Persistent organochlorine pollutants, such as polychlorinated biphenyls (PCBs) and organochlorine pesticides, are found in the general population and tend to accumulate in blood and tissues. Their distribution was examined in the starting plasma pools for fractionation, Cohn plasma fractions and therapeutic concentrates. MATERIALS AND METHODS: In each process fraction, total protein, cholesterol and triglycerides were measured as well as organochlorine pesticides and PCB congeners. RESULTS: Organochlorine compounds were undetectable in cryoprecipitate, Cohn fraction I, Factor VIII and immunoglobulin concentrates, and reduced in albumin preparations. CONCLUSION: Cohn plasma fractionation is very efficient for removing pollutants present in the starting material. Biological processing techniques should be analysed for their capacity to eliminate/reduce persistent organochlorine pollutants from the therapeutic proteins.
Asunto(s)
Factor VIII/normas , Inmunoglobulinas Intravenosas/normas , Insecticidas/sangre , Plasma/química , Bifenilos Policlorados/sangre , Albúmina Sérica/normas , Adolescente , Adulto , Anciano , Fraccionamiento Químico , Contaminantes Ambientales/sangre , Contaminantes Ambientales/aislamiento & purificación , Factor VIII/aislamiento & purificación , Factor VIII/uso terapéutico , Humanos , Inmunoglobulinas Intravenosas/aislamiento & purificación , Inmunoglobulinas Intravenosas/uso terapéutico , Insecticidas/aislamiento & purificación , Persona de Mediana Edad , Bifenilos Policlorados/aislamiento & purificación , Albúmina Sérica/aislamiento & purificación , Albúmina Sérica/uso terapéuticoRESUMEN
Concentrations of selected organochlorine pesticides and PCBs in seven fish species (cichlids) from the north end of the Lake Tanganyika, Burundi, Africa were determined. Results were compared to previous work on the Lake Tanganyika and other water bodies and to the European Community maximum residue levels (MRLs) in edible fat. The analytical method included a hot Soxhlet extraction with a mixture of acetone: hexane (1:3, v/v), gravimetrically lipid determination, and a single step clean-up. For PCBs and stable pesticides, the clean-up was done on activated silica gel impregnated with concentrated sulfuric acid, while for non acid-stable pesticides superposed layers of alumina, silica and florisil impregnated with 15% methanolic solution of KOH were successively used. Recoveries of organochlorine pesticides from certified reference material (CRM 430) were ranging from 86% for p,p'-DDT to 107% for endrin, while recoveries from blank fat spiked fortified at three different levels were between 65% for alachlor at the lowest fortification level and 107% for mirex at the highest fortification level. The limits of detection for each analyte were ranging from 0.1 ng/g to 0.5 ng/g fat. All chlorinated pesticides were found in the analyzed species but at low concentrations. Boulengerochromis microlepis contained the highest concentrations of HCHs (288.2 +/- 15.5 ng/g fat) and DDTs (909.1 +/- 42.5 ng/g fat), while the highest PCB levels (166.7 +/- 37.4 ng/g fat for the sum of 12 congeners) were found in Oreochromis niloticus. However, there is no evidence that Lake Tanganyika is more contaminated with pesticides than other African water bodies.
Asunto(s)
Peces/metabolismo , Hidrocarburos Clorados/metabolismo , Plaguicidas/metabolismo , Bifenilos Policlorados/metabolismo , Animales , Burundi , Técnicas de Química Analítica/métodos , Cromatografía de Gases , Monitoreo del Ambiente/métodos , Grasas/metabolismo , Hidrocarburos Clorados/química , Plaguicidas/química , Bifenilos Policlorados/química , Contaminantes Químicos del Agua/metabolismo , Contaminación Química del Agua/análisisRESUMEN
OBJECTIVE: Our objective was to study the pharmacokinetics of ibuprofen in premature infants with patent ductus arteriosus on day 3 and day 5 after birth. METHODS: Ibuprofen was administered on days 3, 4, and 5 by a 15-minute intravenous infusion of 10, 5, and 5 mg/kg, respectively, with the aim of closing the ductus arteriosus. Blood samples were drawn at time zero and at 0.5, 1, 2, 4, 12, and 24 hours after the first and third doses. Ibuprofen plasma concentrations were assayed by HPLC. RESULTS: A total of 27 premature infants were included (gestational age, 28.6 +/- 1.9 weeks; birth weight, 1250 +/- 460 g; values are mean +/- standard deviation). Ibuprofen pharmacokinetics followed a 2-compartment open model. Between the first and third doses (day 3 and day 5) there was a significant decrease of the volume of distribution of the central compartment (Vd(c)) (0.244 versus 0.171 L/kg; P =.03) and area under the plasma concentration-time curve (524 versus 447 mg. h/L; P =.01). The decrease in Vd(c) was most pronounced in patients with a closing ductus. Total body clearance and plasma half-life did not change significantly. No significant differences were observed in ibuprofen peak plasma concentrations after the first and third doses in relation to ductal status after treatment. CONCLUSION: Ibuprofen pharmacokinetics showed a large interindividual variation in premature infants during treatment for patent ductus arteriosus, and significant changes may occur between day 3 and day 5 after birth in those infants with a closing ductus. These findings may have implications for the treatment schedule with ibuprofen in patients with patent ductus arteriosus.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacocinética , Conducto Arterial/metabolismo , Ibuprofeno/farmacocinética , Recien Nacido Prematuro , Recién Nacido de muy Bajo Peso , Área Bajo la Curva , Conducto Arterial/patología , Conducto Arterial/cirugía , Edad Gestacional , Humanos , Ibuprofeno/sangre , Recién Nacido , Infusiones Intravenosas , Modelos Estadísticos , Respiración Artificial , Síndrome de Dificultad Respiratoria del Recién Nacido/metabolismoRESUMEN
BACKGROUND: A case-control study was designed to determine the possible association between chronic exposure to dioxins and polychlorinated biphenyls (PCBs), and the occurrence of endometriosis. The study group consisted of 42 infertile endometriosis cases and 27 mechanical infertile controls, both groups attending one of the collaborating Centres for Reproductive Medicine, enrolled between 1996-1998. METHODS: Exposure assessment to dioxin-like compounds was determined through CALUX (chemical-activated luciferase gene expression)-bioassay to measure dioxin-like total toxic equivalents (dioxins and co-planar PCBs), whereas non-co-planar PCBs were determined through chemical analysis. RESULTS: No association was found between median dioxin-like total toxic equivalents (TEQ) and the occurrence of endometriosis in infertile women [cases (n = 34): 29; controls (n = 27): 24; NS]. When patients were subdivided based on an arbitrary cut-off value of 100 pg TEQ/g serum lipids, no statistically significant association between very high exposure to dioxin-like compounds and endometriosis was found [crude odds ratio (OR) = 4.33; confidence interval (CI) 0.49-38.19; NS]. After adjusting for body mass index, and alcohol consumption, the risk increased slightly to OR = 4.6 (CI 0.48-43.62; NS). There was no confounding by age, ovulatory dysfunction, caffeine intake, smoking or exposure to non-co-planar PCBs. CONCLUSIONS: The study results showed no statistically significant association between exposure to dioxin-like compounds and the occurrence of endometriosis in infertile women.
Asunto(s)
Dioxinas/efectos adversos , Endometriosis/inducido químicamente , Endometriosis/complicaciones , Contaminantes Ambientales/efectos adversos , Infertilidad Femenina/etiología , Bifenilos Policlorados/efectos adversos , Adulto , Animales , Bioensayo , Estudios de Casos y Controles , Dioxinas/sangre , Contaminantes Ambientales/sangre , Femenino , Humanos , Infertilidad Femenina/sangre , Bifenilos Policlorados/sangre , Estudios Prospectivos , Ratas , Factores de Riesgo , Células Tumorales CultivadasRESUMEN
The combination of narrow-bore capillary gas chromatography with bench-top quadrupole mass spectrometric detection was evaluated for the determination of polychlorinated biphenyls (PCBs). The qualitative and quantitative performances of the system are illustrated by several analyses (PCB standards and human milk extracts). Capillary columns with different internal diameters (0.10, 0.18 and 0.22 mm, respectively) were compared for their ability to separate PCB congeners and the analysis time. Short run times (less than 7 min) were sufficient for complete separation of PCB congeners on a 0.10-mm internal diameter (I.D.) capillary column without any loss of resolution when compared with a 0.22 mm I.D. column. Good qualitative and quantitative data acquisition was possible with quadrupole mass spectrometer for run times of 8 min, but incomplete peak reading was observed when run times were reduced to 3-4 min. Selected ion monitoring and dwell times of 10 ms are necessary to obtain detection limits for individual PCB congeners as low as 0.4 pg microl(-1) for standard solutions and 0.2 ng g(-1) fat for milk extracts. By using cold splitless injection, relatively high volumes (1 microl) for narrow-bore capillaries could be injected without any peak distortion.