Somatic embryogenesis in the commercial papaya hybrid UENF/Caliman 01 relying on plantlet production from sexed adult hermaphrodite donor plants.

Somatic embryogenesis from explants from hermaphrodite papaya mother plants is an alternative for the production of true-to-type plants without the need for sexing. This study aimed to analyze hormonal and osmotic inducers in different somatic embryogenesis stages in the commercial hermaphrodite hybrid papaya UENF/Caliman 01. Leaf disks from in vitro shoots originated from ex vitro hermaphrodite plants were cultured in induction medium supplemented with different concentrations of 2,4-D (6, 9, 12, 15, and 18 µM) and 4-CPA (19, 22, 25, 28, and 31 µM). After 90 days, the formation of somatic embryos was verified. The 2,4-D induced the formation of light brown calli with low frequency (20%) of somatic embryogenesis. However, 4-CPA (25 µM) induced 96% of embryogenic calli, which were transferred to maturation medium (MM) and cultured for 30 days. The MM contained ABA (0.5 µM) and AC (15 g L-1) and produced 36.6 somatic embryos callus-1, mainly on cotyledonary stage. Cotyledonary embryos were transferred to germination medium supplemented with gibberellic acid (GA3) (0.0, 1.44, 2.88, and 4.32 µM), and the conversion into plantlets was enhanced with GA3 at 2.88 µM.

Miniestaquia de mamoeiro hermafrodita ‘Golden com indutor auxínico, em sistema semi-hidropônico / Mini-cutting of hermaphrodite papaya 'Golden' with auxin inductor in semi-hydroponic system

O mamoeiro tradicionalmente é propagado por sementes. A propagação assexuada é uma alternativa para a produção de plantas hermafroditas com o padrão de frutos exigidos pelo mercado consumidor. Sendo assim, objetivou-se estudar a propagação vegetativa em mamoeiro hermafrodita ‘Golden, testando o ácido indol-3-butírico (AIB) no enraizamento de miniestacas, em sistema semi-hidropônico. O delineamento experimental utilizado foi o inteiramente casualizado. Os tratamentos consistiram de cinco concentrações de AIB (0, 3, 6, 9 e 12 mg L-1 ), com quatro repetições de seis miniestacas retiradas de plantas hermafroditas em cada parcela. Após 60 dias do cultivo, as miniestacas foram analisadas quanto à porcentagem de enraizamento, porcentagem de sobrevivência, comprimento da maior raiz, e número de folhas. A concentração em torno de 9 mg L-1 de AIB propiciou uma melhor indução ao enraizamento das miniestacas, bem como maior sobrevivência das mudas, formação de folhas e qualidade de suas raízes. Recomenda-se o uso de 9 mg L-1 de AIB no sistema semi-hidropônico de produção de mudas usando miniestacas de mamoeiro ‘Golden hermafrodita.(AU)

Papaya is traditionally propagated by seeds. Asexual propagation is an alternative for the production of hermaphrodite plants, with the fruit pattern required by the consumer market. The objective of this study was to study the vegetative propagation in 'Golden' hermaphrodite papaya, by testing the indole-3-butyric acid (IBA) in the rooting of mini-cuttings in a semi-hydroponic system. The experimental design was completely randomized. The treatments consisted of five concentrations of IBA (0, 3, 6, 9, and 12 mg L-1 ), with four replicates of six mini-cuttings taken from hermaphrodite plants in each plot. After 60 days of cultivation, the mini-cuttings were analyzed for rooting percentage, survival percentage, length of the largest root, and the number of leaves. The concentration around 9 mg L -1 of IBA provided a better induction to the rooting of mini-cuttings, as well as greater seedling survival, leaf formation, and root quality. The use of 9 mg L-1 of IBA in the semi-hydroponic system of seedling production is recommended using 'Golden' hermaphrodite papaya mini-cuttings.(AU)

Miniestaquia de mamoeiro hermafrodita ‘Golden’ com indutor auxínico, em sistema semi-hidropônico / Mini-cutting of hermaphrodite papaya 'Golden' with auxin inductor in semi-hydroponic system

O mamoeiro tradicionalmente é propagado por sementes. A propagação assexuada é uma alternativa para a produção de plantas hermafroditas com o padrão de frutos exigidos pelo mercado consumidor. Sendo assim, objetivou-se estudar a propagação vegetativa em mamoeiro hermafrodita ‘Golden’, testando o ácido indol-3-butírico (AIB) no enraizamento de miniestacas, em sistema semi-hidropônico. O delineamento experimental utilizado foi o inteiramente casualizado. Os tratamentos consistiram de cinco concentrações de AIB (0, 3, 6, 9 e 12 mg L-1 ), com quatro repetições de seis miniestacas retiradas de plantas hermafroditas em cada parcela. Após 60 dias do cultivo, as miniestacas foram analisadas quanto à porcentagem de enraizamento, porcentagem de sobrevivência, comprimento da maior raiz, e número de folhas. A concentração em torno de 9 mg L-1 de AIB propiciou uma melhor indução ao enraizamento das miniestacas, bem como maior sobrevivência das mudas, formação de folhas e qualidade de suas raízes. Recomenda-se o uso de 9 mg L-1 de AIB no sistema semi-hidropônico de produção de mudas usando miniestacas de mamoeiro ‘Golden’ hermafrodita.

Papaya is traditionally propagated by seeds. Asexual propagation is an alternative for the production of hermaphrodite plants, with the fruit pattern required by the consumer market. The objective of this study was to study the vegetative propagation in 'Golden' hermaphrodite papaya, by testing the indole-3-butyric acid (IBA) in the rooting of mini-cuttings in a semi-hydroponic system. The experimental design was completely randomized. The treatments consisted of five concentrations of IBA (0, 3, 6, 9, and 12 mg L-1 ), with four replicates of six mini-cuttings taken from hermaphrodite plants in each plot. After 60 days of cultivation, the mini-cuttings were analyzed for rooting percentage, survival percentage, length of the largest root, and the number of leaves. The concentration around 9 mg L -1 of IBA provided a better induction to the rooting of mini-cuttings, as well as greater seedling survival, leaf formation, and root quality. The use of 9 mg L-1 of IBA in the semi-hydroponic system of seedling production is recommended using 'Golden' hermaphrodite papaya mini-cuttings.

Morphophysiology, Phenotypic and Molecular Diversity of Auxin-induced Passiflora mucronata Lam. (Passifloraceae).

Genetic diversity allows identification of potential intraspecific genotypes in the genus Passiflora. The objective of this study was to examine the morphological and genetic diversity of auxin-induced Passiflora mucronata. The experiments were arranged in a complete randomized block design, with a 9 x 2 factorial arrangement (nine genotypes x presence and absence of auxin, indole-3-butyric acid (IBA)), with four replicates of 16 cuttings. The rooting and vegetative growth responses were variable. Genotype 5 was more responsive in the absence of IBA and genotypes 3, 8 and 9 were more responsive in the presence of IBA. Auxin increased rooting rate and percentage, reducing the average time of root protrusion in eight days. IBA also contributed to increase photosynthesis and dry root and shoot mass in 55.55 and 44.44% of the genotypes, respectively. The highest relative contribution to phenotypic diversity in the absence of auxin was rate (38.75%) and percentage (20.27%) of rooting, whereas in the presence of auxin was stomatal conductance (23.19%) and root dry mass (20.91%). Similarity was found for phenotypic and molecular divergence in the presence of IBA, in which genotypes 1 and 6; genotypes 5, 8 and 9; and genotype 3 were clustered in distinct groups.