Inhibition of semicarbazide-sensitive amine oxidases decreases lymphocyte infiltration in the early phases of rat liver allograft rejection.

Vascular adhesion protein-1 (VAP-1) has been shown to mediate lymphocyte adhesion to endothelia at sites of inflammation in vitro and in vivo. VAP-1 is also an ectoenzyme with semicarbazide-sensitive amine oxidase (SSAO) activity. In this study we investigated whether inhibition of SSAO influences the inflammatory infiltration in acute rat liver allograft rejection. BN recipients of DA liver allografts were treated with 50 mg/kg/d semicarbazide, an inhibitor of SSAO, or similar volumes of saline. 10 rats/group were followed for graft survival, and 10 rats/group were sacrificed on day 7 post-transplantation for histology and T-lymphocyte isolation. The area percentage of portal inflammatory infiltrates in the grafts was assessed from digital photomicrographs. The proportion of CD4-, CD8- and IL2-receptor positive lymphocytes in the graft was quantified with flow cytometry. On day 7, semicarbazide treatment significantly decreased the inflammatory infiltrate area in the grafts. CD4-, CD8- and IL2-receptor positive cells were equally affected. However, animal survival was not affected. Blockade of the enzymatic activity of VAP-1 has a significant effect on lymphocyte infiltration early in acute liver rejection. Later, activation of other adhesion pathways can by-pass the blockade caused by VAP-inhibition.

[Not Available]. / Hygiene et Assainissement des Villes: zur wissenschaftlichen Begrundung der 'Stadthygiene' des 19. Jahrhunderts und deren Konsequenzen.

By studying the etiology and the causalities of the cholera breaking out in the German cities since the 1830s the young scientific discipline "Hygiene" tried to influence urban administrations to prevent the contamination of the soil with human excrements. Most of the Hygienists followed the theory developed by Max von Pettenkofer, the first German professor of Hygiene, that the soiling was the causing factor of most of the intestinal diseases. This theory based on the hypothesis of a prerequisite fermentation of a germ in the soil before being capable to produce disease in a susceptible individual. According to this - never irrefutably proved - theory the cities should be aware of the condition of soil (Bodenbeschaffenheit), whereas the pollution of the running water was not considered to be dangerous by reason of the supposed self-purification of running streams.

Flow cytometric DNA measurements in aspiration biopsies and surgical specimens of breast cancer.

OBJECTIVE: One of the prognostic factors in breast cancer is the proliferation activity of the tumor. This study sought knowledge of this activity before surgery to benefit the design and timing of therapy. STUDY DESIGN: Flow cytometric DNA analysis data from 52 diagnostic fine needle aspirates were compared with data from subsequent surgical specimens. RESULTS: The data showed that the coefficient of variation of the G1 peak was lower in the aspirates. Small, near-diploid peaks were detected more frequently in aspirate histograms than in surgical specimens. DNA analyses by flow cytometry from aspirates, which can be obtained prior to surgical treatment, were as reliable as those obtained from surgical specimens, provided that the cellular material was diagnostic of cancer. CONCLUSION: Our results suggest that flow cytometry DNA analysis from the first preoperative cytologic specimen from a breast tumor will permit faster planning and coordination of breast cancer care.

[Mortality of patients with differentiated thyroid carcinoma in Norway. The influence of sex, age, tumor stage, histologic type and residence at the time of diagnosis]. / Overlevelse ved differensiert thyreoideakarsinom i Norge. Betydningen av kjønn, alder, tumor-stadium, histologisk type og bosted ved diagnose.

A multivariate analysis of relative survival and prognostic factors in differentiated thyroid cancer, based on all 2,625 cases reported to the Cancer Registry of Norway 1970-85, showed that age and tumour stage had a strong and individual impact on survival, while sex and histology had only a minor influence. The incidence of thyroid cancer has previously been shown to differ between different geographical locations and types of community in Norway, being highest in Northern Norway and in fishing communities, compared with southern parts and agricultural and industrial communities. These residence-related features had no influence on survival, implying that prognosis may depend on other factors than place of residence.

Survival and prognostic factors in differentiated thyroid cancer--a multivariate analysis of 1,055 cases.

Survival (5- and 10-year) and prognostic factors of all differentiated thyroid cancer patients (n = 1,055) occurring in Norway in 1970-79 are presented. The multivariate analysis (GLIM) revealed that stage and age were the only significant prognostic factors. Sex and histological type could not be proved to be of major prognostic value. The decline in relative survival with age was different in the three stages, appearing as a continuous decrease in stage 3, while in stage 1 the decrease was present only in patients older than 75 years.